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The map label for this gene is thrA [C]

Identifier: 94985229

GI number: 94985229

Start: 1198797

End: 1200209

Strand: Reverse

Name: thrA [C]

Synonym: Dgeo_1127

Alternate gene names: 94985229

Gene position: 1200209-1198797 (Counterclockwise)

Preceding gene: 94985230

Following gene: 94985226

Centisome position: 48.65

GC content: 69.36

Gene sequence:

>1413_bases
ATGGCGTATTCGCTCCTCGTGATGAAGTTCGGCGGCACCAACATGCAGGATGCCCAGGCGATCCGCCACAGCGCCTCGCT
GGCGGCCCGCAGCATCCGCGAAGGCGTCAAGGTGGTGGTGGTGGTGTCCGCGATGGCAGGCGTGACCAATCAGCTGCTGG
GGCTGGCCGACGCGGCGCAGCGCGGCGATATCGCAACGGCCAACGATGAGATTGCGGCGCTGCGCACCCGCCACTTTGCA
GCGGCCCAGGAACTCGGCGCAGCGCCCGATAGTGAGGTGGTGCGTGAGATCCGCGAGCTGCACGAGACGCTGCGCCAGGC
CGTGTATGGCGTGTACCTGCTGCGCGAACTCACCCCCCGCAGCCGCGACCTGATTGTCGCCTTTGGTGAGCGGTTCAGCG
CCCCGCTGATGCGTCTGGCGCTGGAGGGGCAGGGCCTTTTGGCCCACCACCTCACCGGCGGGCAGGCGGGCATCACCACG
GACACGCACTTCGGCAACGCGCGTCCCCTGCCGGGTACCTACGAACGCATCCGCGACCGACTGGGCGGCCTGCTGGCAGC
GGGCGTCACACCAGTCGTGGCGGGCTTCATGGGCGAGACCGAGGAGGGGGCCATCACCACCCTGGGCCGCGGCGGCACCG
ACTTTTCTGCCACGATCATCGGCAAGGCGCTGGACGCCGACGAGGTGTGGGCCTGGAAGGACGTGGACGGCGTGATGAGC
GCCGATCCACGCGTCGTGCCAGACGCCCGCAACATCGAGGTCCTCAGCTACGGGGAGGTGATGGAGCTCGCCTACTTTGG
CGCGAAGGTGCTGCATCCCCTCGCGGTGACGCCCCTCCAGGAAAGCGGGATTCCCCTGCGCGTGAAGAGTGCCGCCGATC
CCGACTTTCCCGGCACGCTGGTGCAGGAGGGCGCCCACAACGAGGCGGGCCATCCGGTCAAGGCGGTGACCGCCATCCGC
CGCGTGAGCATCATCAACGTGACCGGCGCTGGGGTGTTGGGCGTGCCGGAGGTGGTGGCCAGCCTGTTCGAGGCCATCGC
CCGCGAGAACATCACCCTGCTGATGGTGTCTCAGAGCAGCTCCATGAGCAATGTCTCGCTGGCGGTGCAGAGCGCCGACG
CCGCGCGGACCCTCGACGCGCTGCGCCGCAGGGTGACCGGCGAGCTGAACATCGAGGAGCAGCCCGGCGTGGCGGTCCTC
GCGATCGTGGGCGCGGGGATGCGCGGTCAGAAGGGGGTGGCCGCGCGGCTGTTTGGCGCGCTCGCCGCTGCCGACGTCAA
CATCCTGATGATCAGCCAGGGGTCCAGCGAGCTGAACATCAGTGTGGCCCTCGAGGACGCCCAGGTTGAGCGGGCCACCC
GCGCCGTTCACGCGGCCTTTGGGCTGGGGCAGACGGCAGCGGCGGCGGACTGA

Upstream 100 bases:

>100_bases
GGGCACCACCCCCCCGCCGGCTCACCCTGTGTTGCCCTGAACGTCCCCCTGCCCAGACGCCCGCCCCCGGCTGCGGCACG
GGGGCGGCATACTGGGCCGC

Downstream 100 bases:

>100_bases
CGGACCGCAGACTAGGGTCTGTCTGGCTGAATTTAGGAAAATGGGGGTATGGGACGGACGGATTTAACCGAGCAACAGTG
GGTGAAATTGGCCCCCCTGC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAYSLLVMKFGGTNMQDAQAIRHSASLAARSIREGVKVVVVVSAMAGVTNQLLGLADAAQRGDIATANDEIAALRTRHFA
AAQELGAAPDSEVVREIRELHETLRQAVYGVYLLRELTPRSRDLIVAFGERFSAPLMRLALEGQGLLAHHLTGGQAGITT
DTHFGNARPLPGTYERIRDRLGGLLAAGVTPVVAGFMGETEEGAITTLGRGGTDFSATIIGKALDADEVWAWKDVDGVMS
ADPRVVPDARNIEVLSYGEVMELAYFGAKVLHPLAVTPLQESGIPLRVKSAADPDFPGTLVQEGAHNEAGHPVKAVTAIR
RVSIINVTGAGVLGVPEVVASLFEAIARENITLLMVSQSSSMSNVSLAVQSADAARTLDALRRRVTGELNIEEQPGVAVL
AIVGAGMRGQKGVAARLFGALAAADVNILMISQGSSELNISVALEDAQVERATRAVHAAFGLGQTAAAAD

Sequences:

>Translated_470_residues
MAYSLLVMKFGGTNMQDAQAIRHSASLAARSIREGVKVVVVVSAMAGVTNQLLGLADAAQRGDIATANDEIAALRTRHFA
AAQELGAAPDSEVVREIRELHETLRQAVYGVYLLRELTPRSRDLIVAFGERFSAPLMRLALEGQGLLAHHLTGGQAGITT
DTHFGNARPLPGTYERIRDRLGGLLAAGVTPVVAGFMGETEEGAITTLGRGGTDFSATIIGKALDADEVWAWKDVDGVMS
ADPRVVPDARNIEVLSYGEVMELAYFGAKVLHPLAVTPLQESGIPLRVKSAADPDFPGTLVQEGAHNEAGHPVKAVTAIR
RVSIINVTGAGVLGVPEVVASLFEAIARENITLLMVSQSSSMSNVSLAVQSADAARTLDALRRRVTGELNIEEQPGVAVL
AIVGAGMRGQKGVAARLFGALAAADVNILMISQGSSELNISVALEDAQVERATRAVHAAFGLGQTAAAAD
>Mature_469_residues
AYSLLVMKFGGTNMQDAQAIRHSASLAARSIREGVKVVVVVSAMAGVTNQLLGLADAAQRGDIATANDEIAALRTRHFAA
AQELGAAPDSEVVREIRELHETLRQAVYGVYLLRELTPRSRDLIVAFGERFSAPLMRLALEGQGLLAHHLTGGQAGITTD
THFGNARPLPGTYERIRDRLGGLLAAGVTPVVAGFMGETEEGAITTLGRGGTDFSATIIGKALDADEVWAWKDVDGVMSA
DPRVVPDARNIEVLSYGEVMELAYFGAKVLHPLAVTPLQESGIPLRVKSAADPDFPGTLVQEGAHNEAGHPVKAVTAIRR
VSIINVTGAGVLGVPEVVASLFEAIARENITLLMVSQSSSMSNVSLAVQSADAARTLDALRRRVTGELNIEEQPGVAVLA
IVGAGMRGQKGVAARLFGALAAADVNILMISQGSSELNISVALEDAQVERATRAVHAAFGLGQTAAAAD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=31.5334773218143, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1790455, Length=462, Percent_Identity=30.5194805194805, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1790376, Length=416, Percent_Identity=27.8846153846154, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320893, Length=496, Percent_Identity=26.008064516129, Blast_Score=176, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49239; Mature: 49108

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSLLVMKFGGTNMQDAQAIRHSASLAARSIREGVKVVVVVSAMAGVTNQLLGLADAAQ
CCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHH
RGDIATANDEIAALRTRHFAAAQELGAAPDSEVVREIRELHETLRQAVYGVYLLRELTPR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRDLIVAFGERFSAPLMRLALEGQGLLAHHLTGGQAGITTDTHFGNARPLPGTYERIRDR
CCCEEEEECCHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LGGLLAAGVTPVVAGFMGETEEGAITTLGRGGTDFSATIIGKALDADEVWAWKDVDGVMS
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCEEECCCCCHHC
ADPRVVPDARNIEVLSYGEVMELAYFGAKVLHPLAVTPLQESGIPLRVKSAADPDFPGTL
CCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHH
VQEGAHNEAGHPVKAVTAIRRVSIINVTGAGVLGVPEVVASLFEAIARENITLLMVSQSS
HHCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCC
SMSNVSLAVQSADAARTLDALRRRVTGELNIEEQPGVAVLAIVGAGMRGQKGVAARLFGA
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
LAAADVNILMISQGSSELNISVALEDAQVERATRAVHAAFGLGQTAAAAD
HHHCCEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
AYSLLVMKFGGTNMQDAQAIRHSASLAARSIREGVKVVVVVSAMAGVTNQLLGLADAAQ
CEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHH
RGDIATANDEIAALRTRHFAAAQELGAAPDSEVVREIRELHETLRQAVYGVYLLRELTPR
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SRDLIVAFGERFSAPLMRLALEGQGLLAHHLTGGQAGITTDTHFGNARPLPGTYERIRDR
CCCEEEEECCHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LGGLLAAGVTPVVAGFMGETEEGAITTLGRGGTDFSATIIGKALDADEVWAWKDVDGVMS
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCCCEEECCCCCHHC
ADPRVVPDARNIEVLSYGEVMELAYFGAKVLHPLAVTPLQESGIPLRVKSAADPDFPGTL
CCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCHH
VQEGAHNEAGHPVKAVTAIRRVSIINVTGAGVLGVPEVVASLFEAIARENITLLMVSQSS
HHCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCC
SMSNVSLAVQSADAARTLDALRRRVTGELNIEEQPGVAVLAIVGAGMRGQKGVAARLFGA
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
LAAADVNILMISQGSSELNISVALEDAQVERATRAVHAAFGLGQTAAAAD
HHHCCEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]