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The map label for this gene is rlmI [H]

Identifier: 94985074

GI number: 94985074

Start: 1036548

End: 1037735

Strand: Reverse

Name: rlmI [H]

Synonym: Dgeo_0969

Alternate gene names: 94985074

Gene position: 1037735-1036548 (Counterclockwise)

Preceding gene: 94985082

Following gene: 94985072

Centisome position: 42.06

GC content: 69.28

Gene sequence:

>1188_bases
ATGACCAAGGCAAGCAAACTCGCCAGCGTGACCCTCAAGCCGGGCGCGCTGCGGCGCATCTCAGGCCGCTACCCCTTCGG
CCACACGGGCGACATCGGGACGGCAGACGCGGGCATCACGCCGGGCGAGGTGGTGGAGGTCCGCGCGCCCGACGGCAGCG
TGATCGGGCGCGGCTACTTCAACCCGGACGGCGCGACCCCCCTGCGAATGCTCACCTGGGACCGCCGGGACATCGACCTG
AACTTCTACCGCGCGCGGGTGCGGGCGGCCCTGGCGCGGCGGGCGGGCCGCATCACGGGGACCAACGCCCTGCGCGTGCT
CCACGCCGAGGCGGACAGACTGCCGGGTGTGGTCGCGGACAGGTTCGGAGACGTGCTGAGCGTGCAGTTGCGGAACGCGG
GCGCCGAGCGCCACCGTGACCTGATCCTACGGGTCTTGCGCGAGGAGACGGGCGCGGCCTCTGCCTTCGAGCGCAGCGAC
ACGAACGAGCGCCGCCGCGAGGGTCTGGACCTGCGGACCGGCGTGCTGTGGGGCGACGTGCCCGAGCGCGTGACCTTTTT
TGAAGATGACCTCACCCTGCACTTCTCCCCGATGGACGCGCAGAAGACCGGCTTTTTCCTCGACCAGCGCGACAACCGCC
GCCTGATGCGAGCGCTCCTCTCACCGGGCGAGGGCTTTCTGGACGTGTATTCCTATACGGGCGGCTTCAGCCTGCATGCG
GCGCGGGCAGGAGCGAAACCGGTCGCGATCGACAAGGATCAGATCGCGCTGGGCGTGTTGGAACGCGAGGCACGCGAGAA
CGCCCTGGACGTTGGCGTGCGCTGGGGCGACGCGCTGGAAACGCTGGCGGCGCTGGAACGCGAGAGGCGGGTGTTTGGCG
CAGCCGTTCTTGACCCACCCACCCTGGCCAAGCGCAAGGAGGACGTGCCACGCGCCAAACGCATCTTTACCGAGGGGGCC
AAGCGGGCGCTGCGCATGCTGAAGCCGGGGGGACACCTCCTGATCAGCACCTGCGCCCACTACATCCGGGTGGATGACCT
GCTCGACGCGTCACGCGTGGCCGCGGGCGAGGCGGGATGTGACGCGGAGGTGGTGACGGTCACCTACCAGCCCGCCGACC
ATCCGCACCTGCTGAGCGTGCCGGAAAGCCTCTACCTCAAGAGCATCCTGCTGCGGAAGGAGGAATAA

Upstream 100 bases:

>100_bases
GCTGGGGGGTGGGGCAAACCGTGCCAAGCAGCCCCTCAACCAGACTCCAACCCCATCCCCAGACCCCCGCCAAAGCCCTC
CCCCCTGCTACCCTGCCCCC

Downstream 100 bases:

>100_bases
GCACAGGGCACCGCACGCTAAAGCCGGTCCAGCAGGACCCCTGACCCAGCCGTGTTGGCAAGCCACCAGAGGGTCACCTC
GCAGGCTTCAATCGGTCTCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTKASKLASVTLKPGALRRISGRYPFGHTGDIGTADAGITPGEVVEVRAPDGSVIGRGYFNPDGATPLRMLTWDRRDIDL
NFYRARVRAALARRAGRITGTNALRVLHAEADRLPGVVADRFGDVLSVQLRNAGAERHRDLILRVLREETGAASAFERSD
TNERRREGLDLRTGVLWGDVPERVTFFEDDLTLHFSPMDAQKTGFFLDQRDNRRLMRALLSPGEGFLDVYSYTGGFSLHA
ARAGAKPVAIDKDQIALGVLEREARENALDVGVRWGDALETLAALERERRVFGAAVLDPPTLAKRKEDVPRAKRIFTEGA
KRALRMLKPGGHLLISTCAHYIRVDDLLDASRVAAGEAGCDAEVVTVTYQPADHPHLLSVPESLYLKSILLRKEE

Sequences:

>Translated_395_residues
MTKASKLASVTLKPGALRRISGRYPFGHTGDIGTADAGITPGEVVEVRAPDGSVIGRGYFNPDGATPLRMLTWDRRDIDL
NFYRARVRAALARRAGRITGTNALRVLHAEADRLPGVVADRFGDVLSVQLRNAGAERHRDLILRVLREETGAASAFERSD
TNERRREGLDLRTGVLWGDVPERVTFFEDDLTLHFSPMDAQKTGFFLDQRDNRRLMRALLSPGEGFLDVYSYTGGFSLHA
ARAGAKPVAIDKDQIALGVLEREARENALDVGVRWGDALETLAALERERRVFGAAVLDPPTLAKRKEDVPRAKRIFTEGA
KRALRMLKPGGHLLISTCAHYIRVDDLLDASRVAAGEAGCDAEVVTVTYQPADHPHLLSVPESLYLKSILLRKEE
>Mature_394_residues
TKASKLASVTLKPGALRRISGRYPFGHTGDIGTADAGITPGEVVEVRAPDGSVIGRGYFNPDGATPLRMLTWDRRDIDLN
FYRARVRAALARRAGRITGTNALRVLHAEADRLPGVVADRFGDVLSVQLRNAGAERHRDLILRVLREETGAASAFERSDT
NERRREGLDLRTGVLWGDVPERVTFFEDDLTLHFSPMDAQKTGFFLDQRDNRRLMRALLSPGEGFLDVYSYTGGFSLHAA
RAGAKPVAIDKDQIALGVLEREARENALDVGVRWGDALETLAALERERRVFGAAVLDPPTLAKRKEDVPRAKRIFTEGAK
RALRMLKPGGHLLISTCAHYIRVDDLLDASRVAAGEAGCDAEVVTVTYQPADHPHLLSVPESLYLKSILLRKEE

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=392, Percent_Identity=29.8469387755102, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1787181, Length=277, Percent_Identity=27.7978339350181, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43536; Mature: 43405

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKASKLASVTLKPGALRRISGRYPFGHTGDIGTADAGITPGEVVEVRAPDGSVIGRGYF
CCCHHHHEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCEE
NPDGATPLRMLTWDRRDIDLNFYRARVRAALARRAGRITGTNALRVLHAEADRLPGVVAD
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCHHHH
RFGDVLSVQLRNAGAERHRDLILRVLREETGAASAFERSDTNERRREGLDLRTGVLWGDV
HCCCEEEEHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCEECCEEECCC
PERVTFFEDDLTLHFSPMDAQKTGFFLDQRDNRRLMRALLSPGEGFLDVYSYTGGFSLHA
CCCEEEEECCEEEEECCCCCCCCCCEECCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEE
ARAGAKPVAIDKDQIALGVLEREARENALDVGVRWGDALETLAALERERRVFGAAVLDPP
HHCCCCCEEECCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCC
TLAKRKEDVPRAKRIFTEGAKRALRMLKPGGHLLISTCAHYIRVDDLLDASRVAAGEAGC
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAEVVTVTYQPADHPHLLSVPESLYLKSILLRKEE
CCEEEEEEECCCCCCCEECCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKASKLASVTLKPGALRRISGRYPFGHTGDIGTADAGITPGEVVEVRAPDGSVIGRGYF
CCHHHHEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCEE
NPDGATPLRMLTWDRRDIDLNFYRARVRAALARRAGRITGTNALRVLHAEADRLPGVVAD
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCHHHH
RFGDVLSVQLRNAGAERHRDLILRVLREETGAASAFERSDTNERRREGLDLRTGVLWGDV
HCCCEEEEHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCEECCEEECCC
PERVTFFEDDLTLHFSPMDAQKTGFFLDQRDNRRLMRALLSPGEGFLDVYSYTGGFSLHA
CCCEEEEECCEEEEECCCCCCCCCCEECCCCCHHHHHHHHCCCCCCEEEEECCCCCEEEE
ARAGAKPVAIDKDQIALGVLEREARENALDVGVRWGDALETLAALERERRVFGAAVLDPP
HHCCCCCEEECCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCC
TLAKRKEDVPRAKRIFTEGAKRALRMLKPGGHLLISTCAHYIRVDDLLDASRVAAGEAGC
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DAEVVTVTYQPADHPHLLSVPESLYLKSILLRKEE
CCEEEEEEECCCCCCCEECCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA