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The map label for this gene is insQ [H]

Identifier: 94985035

GI number: 94985035

Start: 992283

End: 993635

Strand: Reverse

Name: insQ [H]

Synonym: Dgeo_0928

Alternate gene names: 94985035

Gene position: 993635-992283 (Counterclockwise)

Preceding gene: 94985037

Following gene: 94985034

Centisome position: 40.27

GC content: 56.17

Gene sequence:

>1353_bases
GTGAAGATTACGCTCACCGCCAAACTGAAGTTGCGGCATACTCCAGAGCAGAAGAAAGCTCTGGACGCCGTAACTTTGGC
GTACCGTGACGCGCTGAACTTCACCTCCCAGAAGGCGTTTGAAATGGGTAAAACCAGCAACGCCGCCAAGATCCAGAGAG
AGGTGTACGCAACTCTGCGGGAAAGGTTCGGGCTGGGCGCTCAAATGGCCTGCTCCGTGCCGCGTTACGTTGGAGCCGTC
TACAAAACTCTCTGGGCGAAGGCCAAACAGAGTAAGGCAGCGCGTGAGGTCAACCCCAAAGCCAAGCGGTATAAGGGACT
GGACAACGCGCCCAAGTTCGTTTCTCGCACCCTGAGCTATCAGTACCAGCGCGATTACTCGTTCAAGAAAGGCCAGCAGG
TGAGTATTTCCACCCTGAATGGCCGTGTGGTGTTGCCCTATGAAGGCTACGCCAAACACCTTGAATACATCGCGCAGGGG
GCTGAAATTGGGGCAGGCAAACTCTGGTACAGCAAAGCCAAAAAGCAGTATTTCCTTTTGGTTCCGCTGACCTTTGAATT
GCCCGACCCCGACCCCATGACCCATAAACAGGTGGTGGGGGTGGATGTGGGAATGCGCTACTTTGCCACTGCATCGAACA
CCTCTGGGAAAGCTCTTTTCAAGTCGGGTAAGGCCACGCTGCGTAAAGCAGAGCGGTTCCAAAAGGCCCGCAAATCGCTT
CAGCAAAAAGGCACTCGTTCCGCCGTGCGCCGTTTGGTGCAACTATCGGGCCGAGAAAGACGGTTTATTGCTGAGACCAA
CCACGCCATCGCCGCTGAAATCCTTTCCCGCTTCCCAAATGCTTTTATCGGCGTCGAGGAGCTGACCGGAGTCCGTGACC
GCACCGAACGGCGCAGCCGGAAGAACAGTTCCGAGAAAACCCGGAAGGCAAACCGTAGACGTGCAAGGTGGAGTTACGCC
GAGTTGCTGAGCTTCCTGGCGTACAAAGCACCGTTGCACGGTTCTATGGTGGTTAAAGTTGACGCCCATTACACCAGCCA
AACCTGCCCCAGGTGTGGACACTGCTCAAGAGAGAACAGGCCGCACAAGGGGCTGATGTTTATCTGTGAAAGCTGTGGGT
ATCAGTTGCACGCTGACCTTGTAGGGGCAAGGAACATAGGACTCAGGGCATTGCTTGTCCGGCAAGACTGGGCAAGCACG
GGGTGTTTGTCATGCACCCCTGGTCGCCCTCAAAGCGCAGACCCGCAAGCTGGGTCTGCTGGGCGAGATGTGTCGGACGC
TGAAGCCAAAGCTGAGCGCCTGAAAAGGTACTCAGAATTGCGGTGGAGTCCAGACGCAAGCCCCCGCCTTTAG

Upstream 100 bases:

>100_bases
GTAATCCCGGAAGCCCCTGCCTTTAGGCATGGGGAGGAAAGGGATTTGACGCCGTAGGCGGCACTTGCCAGTTCATATAT
AGTCATGATAGGCTCCCTAT

Downstream 100 bases:

>100_bases
GCGCGGGGTCATGACCCCCTCAGGAGAACCATGAACCTCATCAAATCCAAACAGCGTGTGGCCGACCACGGCGAGGTCTT
TACCCCGCCCTGGTTGGTGG

Product: transposase, IS605 OrfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MKITLTAKLKLRHTPEQKKALDAVTLAYRDALNFTSQKAFEMGKTSNAAKIQREVYATLRERFGLGAQMACSVPRYVGAV
YKTLWAKAKQSKAAREVNPKAKRYKGLDNAPKFVSRTLSYQYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQG
AEIGAGKLWYSKAKKQYFLLVPLTFELPDPDPMTHKQVVGVDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSL
QQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSRKNSSEKTRKANRRRARWSYA
ELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENRPHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWAST
GCLSCTPGRPQSADPQAGSAGRDVSDAEAKAERLKRYSELRWSPDASPRL

Sequences:

>Translated_450_residues
MKITLTAKLKLRHTPEQKKALDAVTLAYRDALNFTSQKAFEMGKTSNAAKIQREVYATLRERFGLGAQMACSVPRYVGAV
YKTLWAKAKQSKAAREVNPKAKRYKGLDNAPKFVSRTLSYQYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQG
AEIGAGKLWYSKAKKQYFLLVPLTFELPDPDPMTHKQVVGVDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSL
QQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSRKNSSEKTRKANRRRARWSYA
ELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENRPHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWAST
GCLSCTPGRPQSADPQAGSAGRDVSDAEAKAERLKRYSELRWSPDASPRL
>Mature_450_residues
MKITLTAKLKLRHTPEQKKALDAVTLAYRDALNFTSQKAFEMGKTSNAAKIQREVYATLRERFGLGAQMACSVPRYVGAV
YKTLWAKAKQSKAAREVNPKAKRYKGLDNAPKFVSRTLSYQYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQG
AEIGAGKLWYSKAKKQYFLLVPLTFELPDPDPMTHKQVVGVDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSL
QQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSRKNSSEKTRKANRRRARWSYA
ELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENRPHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWAST
GCLSCTPGRPQSADPQAGSAGRDVSDAEAKAERLKRYSELRWSPDASPRL

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=276, Percent_Identity=26.8115942028986, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 50598; Mature: 50598

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITLTAKLKLRHTPEQKKALDAVTLAYRDALNFTSQKAFEMGKTSNAAKIQREVYATLR
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ERFGLGAQMACSVPRYVGAVYKTLWAKAKQSKAAREVNPKAKRYKGLDNAPKFVSRTLSY
HHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
QYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQGAEIGAGKLWYSKAKKQYFLL
HHHCCCCCCCCCEEEEEEECCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
VPLTFELPDPDPMTHKQVVGVDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSL
EEEEEECCCCCCCCCCEEEEEECCHHEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
QQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSR
HHCCHHHHHHHHHHHCCCCCEEEHHCCHHHHHHHHHHCCCHHCCHHHHHCHHHHHHHHHH
KNSSEKTRKANRRRARWSYAELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
PHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWASTGCLSCTPGRPQSADPQAGSA
CCCHHHHHHHHCCCEEEHHHHCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCC
GRDVSDAEAKAERLKRYSELRWSPDASPRL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKITLTAKLKLRHTPEQKKALDAVTLAYRDALNFTSQKAFEMGKTSNAAKIQREVYATLR
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ERFGLGAQMACSVPRYVGAVYKTLWAKAKQSKAAREVNPKAKRYKGLDNAPKFVSRTLSY
HHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHH
QYQRDYSFKKGQQVSISTLNGRVVLPYEGYAKHLEYIAQGAEIGAGKLWYSKAKKQYFLL
HHHCCCCCCCCCEEEEEEECCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEE
VPLTFELPDPDPMTHKQVVGVDVGMRYFATASNTSGKALFKSGKATLRKAERFQKARKSL
EEEEEECCCCCCCCCCEEEEEECCHHEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHH
QQKGTRSAVRRLVQLSGRERRFIAETNHAIAAEILSRFPNAFIGVEELTGVRDRTERRSR
HHCCHHHHHHHHHHHCCCCCEEEHHCCHHHHHHHHHHCCCHHCCHHHHHCHHHHHHHHHH
KNSSEKTRKANRRRARWSYAELLSFLAYKAPLHGSMVVKVDAHYTSQTCPRCGHCSRENR
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
PHKGLMFICESCGYQLHADLVGARNIGLRALLVRQDWASTGCLSCTPGRPQSADPQAGSA
CCCHHHHHHHHCCCEEEHHHHCCCCCCEEEEEEECCCCCCCCEECCCCCCCCCCCCCCCC
GRDVSDAEAKAERLKRYSELRWSPDASPRL
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]