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The map label for this gene is hisZ [H]

Identifier: 94984890

GI number: 94984890

Start: 841205

End: 842392

Strand: Reverse

Name: hisZ [H]

Synonym: Dgeo_0783

Alternate gene names: 94984890

Gene position: 842392-841205 (Counterclockwise)

Preceding gene: 94984894

Following gene: 94984889

Centisome position: 34.14

GC content: 70.03

Gene sequence:

>1188_bases
ATGCCACATCCCCCTTCTCCCAGTGCTTTAATGCCCCCCGTGAGTCTTCCTTCCCGCCCCATCCCCCTGTCCCGCGCCGT
TCCCCAGGGCACCCGCGACGTGCTGCCGCCCGAGTGGGCCTGGCGTGAGCATTTGCGGACGCGGCTGGCGGGCGTGTTCG
CCGCCTGGGGCTACCGGGGCGTGGAAGTCCCGGCGCTGGAGTTTGCCTCCGAAGCTCATCCGCAAAACGCTCGGGCTTTT
AAGCTGATCGATACGGACGGGGAGGTGCTGGCTCTGCGCAGCGAGTTCACCACCGCGATCGGGCGGCTGGTGCAGGCCCG
TTTCCCGGAGGGGCCGTTTCCGCTGCGTCTCCAGTACGGAGGCCGCCTGTGGCTGCGGACCCTCACGAGCGAGCTCGGAC
GGCTGCGCGAGTTCGGTCAGGTCGGCGTGGAGCTGATCGGCGTGGCGACCCCGCAAGCCGACGCGGAACTGCTGCAGCTG
GGCACGCAGGCACTCGACACGGTGGGCGTGCAGGCCGTGATGGAGGTGGGGTATCCCGGCTTTGTGGACGCGGTGTTGGA
GGACGCCGGGCTACCTGCTTCTGCCCGGAATGCCCTCCACGACGCGATTGACCGCAAAAGCGGCGCGGACGTGGACCTGC
TGGCCGCTCGGCATGGCCTGAATTCCGCGGTGACGGCCACCCTCCACACCCTCACCGACCTGTATGGTGGTCCCGAGGTC
CTGGCACAGGCAGCCGCGCTGGCCCGCGGCACACGGGCACGGGCGGCGGTGGCGCATCTGGAGGCGGTGGCCGCGCTGTA
TGCACCGGCCCCAGATGCGGCAGGGTTGCTGTTTGACCTGGGCGCCAGCCGCCGCTACGGGTACTACACCGGAATCACCT
TCCGGGCCTACGCGGAGGGCCTGAACCAGCCGGTGCTGGGGGGAGGGCGCTACGATCTGGGCGGGCTGCCCGGCGCGGGC
TTTGCCATCGGCCTGGAGCGGCTGACCGAGGTGACAGCACCACAGTTGCCCCCCGAGCCGGAGGTGGTGCTGGCCCTCGA
CCGAGCGGGAGCCGAACACGCCCGCTCGCTGGGGCTGAGCGCCGAACTCGCCTGGACCGACAACCAAGTAGAACTGCTCG
CCTACTGCCAGAAACGGGGCATCCGCCGCTGGGCGCAGGGCGACACGCTGGTGGAGGTGACCGCATGA

Upstream 100 bases:

>100_bases
GAGCAGGAGGAGGGCGAGAGGAGCACGGCGCATGGGGGGAGTATAGGGTGGTCAGCCCTGAGCGGTCAGCTCTCCTTCGC
CGAATGTTGATGCGCTCCAC

Downstream 100 bases:

>100_bases
CGCCCGCGCCAGCGCGTGACCCGGACCACCTCACCCTGGCGCTTCCGAAGGGCCGCATCCTGGAAGACGCAGTGACCCTG
CTCGCGCGCGCCGGACTGCC

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPHPPSPSALMPPVSLPSRPIPLSRAVPQGTRDVLPPEWAWREHLRTRLAGVFAAWGYRGVEVPALEFASEAHPQNARAF
KLIDTDGEVLALRSEFTTAIGRLVQARFPEGPFPLRLQYGGRLWLRTLTSELGRLREFGQVGVELIGVATPQADAELLQL
GTQALDTVGVQAVMEVGYPGFVDAVLEDAGLPASARNALHDAIDRKSGADVDLLAARHGLNSAVTATLHTLTDLYGGPEV
LAQAAALARGTRARAAVAHLEAVAALYAPAPDAAGLLFDLGASRRYGYYTGITFRAYAEGLNQPVLGGGRYDLGGLPGAG
FAIGLERLTEVTAPQLPPEPEVVLALDRAGAEHARSLGLSAELAWTDNQVELLAYCQKRGIRRWAQGDTLVEVTA

Sequences:

>Translated_395_residues
MPHPPSPSALMPPVSLPSRPIPLSRAVPQGTRDVLPPEWAWREHLRTRLAGVFAAWGYRGVEVPALEFASEAHPQNARAF
KLIDTDGEVLALRSEFTTAIGRLVQARFPEGPFPLRLQYGGRLWLRTLTSELGRLREFGQVGVELIGVATPQADAELLQL
GTQALDTVGVQAVMEVGYPGFVDAVLEDAGLPASARNALHDAIDRKSGADVDLLAARHGLNSAVTATLHTLTDLYGGPEV
LAQAAALARGTRARAAVAHLEAVAALYAPAPDAAGLLFDLGASRRYGYYTGITFRAYAEGLNQPVLGGGRYDLGGLPGAG
FAIGLERLTEVTAPQLPPEPEVVLALDRAGAEHARSLGLSAELAWTDNQVELLAYCQKRGIRRWAQGDTLVEVTA
>Mature_394_residues
PHPPSPSALMPPVSLPSRPIPLSRAVPQGTRDVLPPEWAWREHLRTRLAGVFAAWGYRGVEVPALEFASEAHPQNARAFK
LIDTDGEVLALRSEFTTAIGRLVQARFPEGPFPLRLQYGGRLWLRTLTSELGRLREFGQVGVELIGVATPQADAELLQLG
TQALDTVGVQAVMEVGYPGFVDAVLEDAGLPASARNALHDAIDRKSGADVDLLAARHGLNSAVTATLHTLTDLYGGPEVL
AQAAALARGTRARAAVAHLEAVAALYAPAPDAAGLLFDLGASRRYGYYTGITFRAYAEGLNQPVLGGGRYDLGGLPGAGF
AIGLERLTEVTAPQLPPEPEVVLALDRAGAEHARSLGLSAELAWTDNQVELLAYCQKRGIRRWAQGDTLVEVTA

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=405, Percent_Identity=25.4320987654321, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24643061, Length=353, Percent_Identity=25.7790368271955, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24643059, Length=372, Percent_Identity=24.7311827956989, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 42086; Mature: 41955

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPPSPSALMPPVSLPSRPIPLSRAVPQGTRDVLPPEWAWREHLRTRLAGVFAAWGYRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VEVPALEFASEAHPQNARAFKLIDTDGEVLALRSEFTTAIGRLVQARFPEGPFPLRLQYG
CCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECC
GRLWLRTLTSELGRLREFGQVGVELIGVATPQADAELLQLGTQALDTVGVQAVMEVGYPG
CHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
FVDAVLEDAGLPASARNALHDAIDRKSGADVDLLAARHGLNSAVTATLHTLTDLYGGPEV
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHCCCHHH
LAQAAALARGTRARAAVAHLEAVAALYAPAPDAAGLLFDLGASRRYGYYTGITFRAYAEG
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEECCHHHHHHHC
LNQPVLGGGRYDLGGLPGAGFAIGLERLTEVTAPQLPPEPEVVLALDRAGAEHARSLGLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCC
AELAWTDNQVELLAYCQKRGIRRWAQGDTLVEVTA
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
>Mature Secondary Structure 
PHPPSPSALMPPVSLPSRPIPLSRAVPQGTRDVLPPEWAWREHLRTRLAGVFAAWGYRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
VEVPALEFASEAHPQNARAFKLIDTDGEVLALRSEFTTAIGRLVQARFPEGPFPLRLQYG
CCCCHHHHHHHCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEEEECC
GRLWLRTLTSELGRLREFGQVGVELIGVATPQADAELLQLGTQALDTVGVQAVMEVGYPG
CHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
FVDAVLEDAGLPASARNALHDAIDRKSGADVDLLAARHGLNSAVTATLHTLTDLYGGPEV
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHHHCCCHHH
LAQAAALARGTRARAAVAHLEAVAALYAPAPDAAGLLFDLGASRRYGYYTGITFRAYAEG
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCEEECCHHHHHHHC
LNQPVLGGGRYDLGGLPGAGFAIGLERLTEVTAPQLPPEPEVVLALDRAGAEHARSLGLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHCCCC
AELAWTDNQVELLAYCQKRGIRRWAQGDTLVEVTA
EEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]