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The map label for this gene is malS [C]

Identifier: 94984767

GI number: 94984767

Start: 716120

End: 717574

Strand: Reverse

Name: malS [C]

Synonym: Dgeo_0660

Alternate gene names: 94984767

Gene position: 717574-716120 (Counterclockwise)

Preceding gene: 94984768

Following gene: 94984766

Centisome position: 29.08

GC content: 67.77

Gene sequence:

>1455_bases
ATGCGCCGCCTTCCCCTCCTCGCCGCGCTGCTCACCTCACTGGCGAGCGCGCAGACCTCCCCCACCCTCCCCTCCTTCGA
GGGGCAGGTGATCTATCAGGTGATGCCTGACCGCTTTGCGGACGGCGACCGGACGAACAACGCGAATGTGAACCGCGCCG
ACCCGCGCGCCTGGCACGGCGGCGATCTGGCAGGGCTAACGGCCAAGCTGCCCTACCTCCGGAAGCTGGGGGCGTCGGCC
GTGTGGCTCACACCCATTTACCGGCAGCAGCTGGCCAACTCCTTTGGAATCGCCCCCTACCACGGCTACTGGCCCGCCGA
CTTTCGCGACGTGGACCCGCATTTCGGAACGCTCGCGGACTTCAAGGAGTTGGTGAAGGCGGCGCATGGGGCAAAGATGC
GCGTGGTCCTCGATCAGGTGATCAATCACTACGGCTACGAGGCGGCGGCGGTGAAGGCGCACCCGGCCTGGTTCAATGGG
AAGGCCGAGTGTGACGCTTCCGGCAACAAGGACGTGGATTGCCCGCTGGCGGGCCTGCCGGACCTCAAACAGTCGAATCC
AGAGGTGCGCGCGCTGCTCTTGGGCAACGCCGACTTCTGGCGGGGGCAGGGGGTGGACGGTTTCCGCTACGACGCGATCA
AGAACGTGGAGGCGCCGTTTTTGAAAGCGCTGCTGGTGCGCGACCGCGCCGCCGGGACATGGACGCTGGGCGAGTGGTAC
GGGGCGGATACCGGGACGGTGGCCGACGGGCAGCGGGCCGGGTTTGACAGCCTCTTCCTCTTCAGCCTGCAACAGGCGCT
GGGGCAAAGCCTCATGGGCGGGCAGGGGCTAAGCCGAGTGGCAAGCGTGCTGAGCCGCCAAGACGAGCTGCCGCGGCCCG
GCGAGGTGGCCCTCTTTCTGGACAACCACGACGTGCCGCGCTTTGCCCAGGGCAGCCTCTTTGAGGACCAGGCTCAGGCC
CGCACCCGCTACGGCCTGCGCGCACTGATGACCCTCAAGGGTGTGCCGGTGATCTGGCAGGGCACCGAGATCGCCCTGCG
CGGCGGCCCCGACCCCGACAACCGCCGCGACATGCGCTTTGAGCAGGAATGGACCCCTGCCGAGCGTCAAGTCTTCGAGA
CGGCGCGTGACGCCATCGCCGTCCGCCGGGCCAACCCGGCCCTTAGCGTCGGGACACAGACGCTGCTGCCCACGCCCGCC
ACCCTGGAAGATGAGCTGCTGCTCTTCATCCGTGAGGCGCAGGGCAAACGCGTACTGGTCGCCTGGCACAACGGCCGGAC
GCGGAAGACGTACAGCCTGCGCCTGAACACCCTGGGCCTCAAGGCGGAGCCGCAGACCATCAGCCGCAGCCTCTTTGCCG
GGCAGGACGCCAAACTCAGCGTGAGCGGGGAATGGCTGCACCTCAGCCTGCCGGGAGAGGACGCGGCGGTGTTTGGGTTG
AGGGAAAAGCACTAG

Upstream 100 bases:

>100_bases
GCGCGGTCTTGCCCCTCCTCGGCCCGCAGGCTGCCCAGTTTGAACAGCCCAGATTCCGGGTCGTCGCCGAACTTGCTGCG
CCTGCCCTTAGCATGTCCCC

Downstream 100 bases:

>100_bases
GCAGGGAGAAGTGCTCGATCTTTTCCTCAGTTCCAACGCCTCATCCCTCGCGCCGCCCTGCTAGCATGCTCCCCGATGCG
TTCCCCGCTGCCCCGCGCTG

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: Beta-amylase; Alpha-amylase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MRRLPLLAALLTSLASAQTSPTLPSFEGQVIYQVMPDRFADGDRTNNANVNRADPRAWHGGDLAGLTAKLPYLRKLGASA
VWLTPIYRQQLANSFGIAPYHGYWPADFRDVDPHFGTLADFKELVKAAHGAKMRVVLDQVINHYGYEAAAVKAHPAWFNG
KAECDASGNKDVDCPLAGLPDLKQSNPEVRALLLGNADFWRGQGVDGFRYDAIKNVEAPFLKALLVRDRAAGTWTLGEWY
GADTGTVADGQRAGFDSLFLFSLQQALGQSLMGGQGLSRVASVLSRQDELPRPGEVALFLDNHDVPRFAQGSLFEDQAQA
RTRYGLRALMTLKGVPVIWQGTEIALRGGPDPDNRRDMRFEQEWTPAERQVFETARDAIAVRRANPALSVGTQTLLPTPA
TLEDELLLFIREAQGKRVLVAWHNGRTRKTYSLRLNTLGLKAEPQTISRSLFAGQDAKLSVSGEWLHLSLPGEDAAVFGL
REKH

Sequences:

>Translated_484_residues
MRRLPLLAALLTSLASAQTSPTLPSFEGQVIYQVMPDRFADGDRTNNANVNRADPRAWHGGDLAGLTAKLPYLRKLGASA
VWLTPIYRQQLANSFGIAPYHGYWPADFRDVDPHFGTLADFKELVKAAHGAKMRVVLDQVINHYGYEAAAVKAHPAWFNG
KAECDASGNKDVDCPLAGLPDLKQSNPEVRALLLGNADFWRGQGVDGFRYDAIKNVEAPFLKALLVRDRAAGTWTLGEWY
GADTGTVADGQRAGFDSLFLFSLQQALGQSLMGGQGLSRVASVLSRQDELPRPGEVALFLDNHDVPRFAQGSLFEDQAQA
RTRYGLRALMTLKGVPVIWQGTEIALRGGPDPDNRRDMRFEQEWTPAERQVFETARDAIAVRRANPALSVGTQTLLPTPA
TLEDELLLFIREAQGKRVLVAWHNGRTRKTYSLRLNTLGLKAEPQTISRSLFAGQDAKLSVSGEWLHLSLPGEDAAVFGL
REKH
>Mature_484_residues
MRRLPLLAALLTSLASAQTSPTLPSFEGQVIYQVMPDRFADGDRTNNANVNRADPRAWHGGDLAGLTAKLPYLRKLGASA
VWLTPIYRQQLANSFGIAPYHGYWPADFRDVDPHFGTLADFKELVKAAHGAKMRVVLDQVINHYGYEAAAVKAHPAWFNG
KAECDASGNKDVDCPLAGLPDLKQSNPEVRALLLGNADFWRGQGVDGFRYDAIKNVEAPFLKALLVRDRAAGTWTLGEWY
GADTGTVADGQRAGFDSLFLFSLQQALGQSLMGGQGLSRVASVLSRQDELPRPGEVALFLDNHDVPRFAQGSLFEDQAQA
RTRYGLRALMTLKGVPVIWQGTEIALRGGPDPDNRRDMRFEQEWTPAERQVFETARDAIAVRRANPALSVGTQTLLPTPA
TLEDELLLFIREAQGKRVLVAWHNGRTRKTYSLRLNTLGLKAEPQTISRSLFAGQDAKLSVSGEWLHLSLPGEDAAVFGL
REKH

Specific function: The precursor protein is proteolytically cleaved to produce multiform beta-amylases and a 48 kDa alpha-amylase after secretion [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=223, Percent_Identity=27.3542600896861, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1789995, Length=520, Percent_Identity=25.3846153846154, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786604, Length=469, Percent_Identity=26.865671641791, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790687, Length=217, Percent_Identity=30.4147465437788, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565753, Length=211, Percent_Identity=27.0142180094787, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=219, Percent_Identity=28.310502283105, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586587, Length=217, Percent_Identity=29.4930875576037, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24586593, Length=220, Percent_Identity=30, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24586599, Length=236, Percent_Identity=27.5423728813559, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330053, Length=407, Percent_Identity=22.3587223587224, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24586591, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583747, Length=256, Percent_Identity=26.5625, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24586597, Length=221, Percent_Identity=27.1493212669683, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24586589, Length=217, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583749, Length=238, Percent_Identity=26.0504201680672, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45549022, Length=221, Percent_Identity=26.6968325791855, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583745, Length=244, Percent_Identity=28.2786885245902, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR005085
- InterPro:   IPR008985
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR001554
- InterPro:   IPR018238
- InterPro:   IPR000125
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF03423 CBM_25; PF01373 Glyco_hydro_14 [H]

EC number: =3.2.1.2; =3.2.1.1 [H]

Molecular weight: Translated: 53263; Mature: 53263

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPLLAALLTSLASAQTSPTLPSFEGQVIYQVMPDRFADGDRTNNANVNRADPRAWHG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GDLAGLTAKLPYLRKLGASAVWLTPIYRQQLANSFGIAPYHGYWPADFRDVDPHFGTLAD
CCCCCHHHCCHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
FKELVKAAHGAKMRVVLDQVINHYGYEAAAVKAHPAWFNGKAECDASGNKDVDCPLAGLP
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCC
DLKQSNPEVRALLLGNADFWRGQGVDGFRYDAIKNVEAPFLKALLVRDRAAGTWTLGEWY
CCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEECCCEE
GADTGTVADGQRAGFDSLFLFSLQQALGQSLMGGQGLSRVASVLSRQDELPRPGEVALFL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
DNHDVPRFAQGSLFEDQAQARTRYGLRALMTLKGVPVIWQGTEIALRGGPDPDNRRDMRF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCCC
EQEWTPAERQVFETARDAIAVRRANPALSVGTQTLLPTPATLEDELLLFIREAQGKRVLV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHEEEEEECCCCEEEE
AWHNGRTRKTYSLRLNTLGLKAEPQTISRSLFAGQDAKLSVSGEWLHLSLPGEDAAVFGL
EEECCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCEEECC
REKH
CCCC
>Mature Secondary Structure
MRRLPLLAALLTSLASAQTSPTLPSFEGQVIYQVMPDRFADGDRTNNANVNRADPRAWHG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
GDLAGLTAKLPYLRKLGASAVWLTPIYRQQLANSFGIAPYHGYWPADFRDVDPHFGTLAD
CCCCCHHHCCHHHHHCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
FKELVKAAHGAKMRVVLDQVINHYGYEAAAVKAHPAWFNGKAECDASGNKDVDCPLAGLP
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCC
DLKQSNPEVRALLLGNADFWRGQGVDGFRYDAIKNVEAPFLKALLVRDRAAGTWTLGEWY
CCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCEEECCCEE
GADTGTVADGQRAGFDSLFLFSLQQALGQSLMGGQGLSRVASVLSRQDELPRPGEVALFL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
DNHDVPRFAQGSLFEDQAQARTRYGLRALMTLKGVPVIWQGTEIALRGGPDPDNRRDMRF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCCC
EQEWTPAERQVFETARDAIAVRRANPALSVGTQTLLPTPATLEDELLLFIREAQGKRVLV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCHHHEEEEEECCCCEEEE
AWHNGRTRKTYSLRLNTLGLKAEPQTISRSLFAGQDAKLSVSGEWLHLSLPGEDAAVFGL
EEECCCCEEEEEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCCCCEEECC
REKH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2435707; 2464578; 2438660; 1827035 [H]