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The map label for this gene is ilvB [H]

Identifier: 94984709

GI number: 94984709

Start: 642652

End: 644373

Strand: Reverse

Name: ilvB [H]

Synonym: Dgeo_0602

Alternate gene names: 94984709

Gene position: 644373-642652 (Counterclockwise)

Preceding gene: 94984710

Following gene: 94984708

Centisome position: 26.12

GC content: 66.78

Gene sequence:

>1722_bases
ATGACGCAGGGGGAGATGACGGGCGCCAAGGCGCTGTGGGCGACGCTGGTCCGGCACGGCATCAACACCGTGTTCGGCTA
TCCGGGTGGGGCGATTATGCCCGTGTATGACGCGCTGACTTTCTACCCAGAGGTCCGGCACATCCTCACGCGCCACGAGC
AGGGGGCCATCCACGCGGCAGAAGGCTGGGCCAAGGCGACCGGCGAAATCGGCGTGTGTCTTGCCACGTCGGGGCCGGGG
GCCACCAACCTGGTGACCGGCCTGGCGGACGCGATGATGGACTCGGTGCCGATCCTCGCCATCACCGGGAACGTGGCACG
GCACCTGATGGGCACCGATGCCTTTCAGGAGGCAGATATCACCGGGATCACGCTGCCCATCACCAAGCACAACTACGTGG
TGCGGGAGGTGGAGGAGTTGCCCCGCATTCTCGCGGAGGCGATCCGCATCGCGCGCAGCGGGCGGCCCGGCCCAGTGCTG
GTCGACATTCCCAAGGACATCCAGCTCGCGCCCTATCACGGAGAGATTGCCGCACCGCATGCGCGGCCAGAAGCCCCCGC
CCCCGCGCCCGAAGCCATTCAGCGTGCCCGCGAGCTGCTGCGAGGAGCAAAAAAGCCCGTTCTCCTCGTGGGCGGCGGCT
CGTTGGATGCAGCGGCAGAGATCACCGCGCTGGCCCGCGCCTGGCATCTTCCGGTGATCACCACCCTGATGGGCTTAGGG
GCCTTTCCAGCGAGCGATCCGCTGTGGCTGGGCATGCCGGGCATGCACGGCTCGGTCGCGGCCAACCGTGCGATCAGCGA
GGCAGACGTGCTGCTGGGTATCGGGCTCCGCTTTGACGACCGGGTGACGGGCCGAGTGAACGGCTTTGCGCCGAATGCCG
CCATCATCCACGTCGAACTCGATGCCGCTGAGATCGGCAAGATCGTGCGAACACACGTGCCGGTGCGCGGGGACGCCGCG
ATCGCCGCCCGGCTGCTGACAGAGGGCGCACAGCGGCTCGACTTGCCGGAATGGCAGGCACAGCTTGAGGCGTGGAAGGC
GCGCACGCAAAAACCCGACCACTGGGGCGCAGCCTCTGCGGTGCAGGCCGTCGTGGAACGCCTGCGCCCCGACGACATCC
TCGCTTCCGACGTGGGACAGCACCAGATGCTGGCCGCACAGCTCGCCCGTTTCGAACGGCCCCGGCGCTGGCTGAACTCG
GGCGGCCTGGGCACCATGGGCTTCGGCTTCCCAGCGGCGATCGGGGCGGCCTTGGCTGAGCCAGGCGTGCGCTCCGTGGT
AATTGCCGGGGACGGCGGTTTTCAGATGACCGCCCAGGAACTCGCCACCCTCAAGATGTACGACATCCGCAACGTCAAGA
TCTGCATCATCAACAATTCCTATCTGGGAATGGTGCGCCAGTGGCAAGAACTCTTCCACGAGCGCCGCTACTCCGAGGTG
TGGTTGGGTGACTCCAATCCCGACTTCCTAAAGCTGGCCGATGCCTACGGCGTTCCTGGCTACCGCGCCGATTGCGCCGA
GGAACTCCCAGCGGCCATCAACGCCTGGCTGGCCGACCCCAAAAGTGCCCTGCTGGAGATTGTGGTGCCCCACGAGCACG
GCGTCTTTCCGATGGTGCCCGCCGGAGCGGCCCTCAGCGAGATGATCGAACAAGAGCCGCCCCGCGTTCCCACGCCGGGG
ATTGAGAAAAATGCCGCCGCCAAGGAGGCACAGGACGCATGA

Upstream 100 bases:

>100_bases
ACTGGAAAACGGCCCGTCCGGAGACAACGCCACGGGCCACCTGCCGCGCCGCGCCGCCTCTTGGTGAGCGTGGCGCGCGC
GCTTTGGCGGGAGGTCAGGC

Downstream 100 bases:

>100_bases
CTGACCCCACCCCACGTGACCACCTCGTCTCCGCCCTGGTGCGCGACGAGCCGCGTGTCCTGACCCGCATCACCTCCCTC
TTCGGGCGGCGCGGGTACAA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTQGEMTGAKALWATLVRHGINTVFGYPGGAIMPVYDALTFYPEVRHILTRHEQGAIHAAEGWAKATGEIGVCLATSGPG
ATNLVTGLADAMMDSVPILAITGNVARHLMGTDAFQEADITGITLPITKHNYVVREVEELPRILAEAIRIARSGRPGPVL
VDIPKDIQLAPYHGEIAAPHARPEAPAPAPEAIQRARELLRGAKKPVLLVGGGSLDAAAEITALARAWHLPVITTLMGLG
AFPASDPLWLGMPGMHGSVAANRAISEADVLLGIGLRFDDRVTGRVNGFAPNAAIIHVELDAAEIGKIVRTHVPVRGDAA
IAARLLTEGAQRLDLPEWQAQLEAWKARTQKPDHWGAASAVQAVVERLRPDDILASDVGQHQMLAAQLARFERPRRWLNS
GGLGTMGFGFPAAIGAALAEPGVRSVVIAGDGGFQMTAQELATLKMYDIRNVKICIINNSYLGMVRQWQELFHERRYSEV
WLGDSNPDFLKLADAYGVPGYRADCAEELPAAINAWLADPKSALLEIVVPHEHGVFPMVPAGAALSEMIEQEPPRVPTPG
IEKNAAAKEAQDA

Sequences:

>Translated_573_residues
MTQGEMTGAKALWATLVRHGINTVFGYPGGAIMPVYDALTFYPEVRHILTRHEQGAIHAAEGWAKATGEIGVCLATSGPG
ATNLVTGLADAMMDSVPILAITGNVARHLMGTDAFQEADITGITLPITKHNYVVREVEELPRILAEAIRIARSGRPGPVL
VDIPKDIQLAPYHGEIAAPHARPEAPAPAPEAIQRARELLRGAKKPVLLVGGGSLDAAAEITALARAWHLPVITTLMGLG
AFPASDPLWLGMPGMHGSVAANRAISEADVLLGIGLRFDDRVTGRVNGFAPNAAIIHVELDAAEIGKIVRTHVPVRGDAA
IAARLLTEGAQRLDLPEWQAQLEAWKARTQKPDHWGAASAVQAVVERLRPDDILASDVGQHQMLAAQLARFERPRRWLNS
GGLGTMGFGFPAAIGAALAEPGVRSVVIAGDGGFQMTAQELATLKMYDIRNVKICIINNSYLGMVRQWQELFHERRYSEV
WLGDSNPDFLKLADAYGVPGYRADCAEELPAAINAWLADPKSALLEIVVPHEHGVFPMVPAGAALSEMIEQEPPRVPTPG
IEKNAAAKEAQDA
>Mature_572_residues
TQGEMTGAKALWATLVRHGINTVFGYPGGAIMPVYDALTFYPEVRHILTRHEQGAIHAAEGWAKATGEIGVCLATSGPGA
TNLVTGLADAMMDSVPILAITGNVARHLMGTDAFQEADITGITLPITKHNYVVREVEELPRILAEAIRIARSGRPGPVLV
DIPKDIQLAPYHGEIAAPHARPEAPAPAPEAIQRARELLRGAKKPVLLVGGGSLDAAAEITALARAWHLPVITTLMGLGA
FPASDPLWLGMPGMHGSVAANRAISEADVLLGIGLRFDDRVTGRVNGFAPNAAIIHVELDAAEIGKIVRTHVPVRGDAAI
AARLLTEGAQRLDLPEWQAQLEAWKARTQKPDHWGAASAVQAVVERLRPDDILASDVGQHQMLAAQLARFERPRRWLNSG
GLGTMGFGFPAAIGAALAEPGVRSVVIAGDGGFQMTAQELATLKMYDIRNVKICIINNSYLGMVRQWQELFHERRYSEVW
LGDSNPDFLKLADAYGVPGYRADCAEELPAAINAWLADPKSALLEIVVPHEHGVFPMVPAGAALSEMIEQEPPRVPTPGI
EKNAAAKEAQDA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=28.1914893617021, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI21361361, Length=495, Percent_Identity=28.0808080808081, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=44.8028673835125, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=42.7065026362039, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786717, Length=534, Percent_Identity=35.5805243445693, Blast_Score=302, Evalue=4e-83,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=27.3224043715847, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI1788716, Length=566, Percent_Identity=27.3851590106007, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17531301, Length=493, Percent_Identity=29.8174442190669, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=493, Percent_Identity=29.8174442190669, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=528, Percent_Identity=25.3787878787879, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=42.3407917383821, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=26.3917525773196, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=531, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=555, Percent_Identity=23.7837837837838, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922626, Length=547, Percent_Identity=27.6051188299817, Blast_Score=157, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61277; Mature: 61146

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGEMTGAKALWATLVRHGINTVFGYPGGAIMPVYDALTFYPEVRHILTRHEQGAIHAA
CCCCCCCHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECC
EGWAKATGEIGVCLATSGPGATNLVTGLADAMMDSVPILAITGNVARHLMGTDAFQEADI
CCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCHHCCCC
TGITLPITKHNYVVREVEELPRILAEAIRIARSGRPGPVLVDIPKDIQLAPYHGEIAAPH
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCCCC
ARPEAPAPAPEAIQRARELLRGAKKPVLLVGGGSLDAAAEITALARAWHLPVITTLMGLG
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AFPASDPLWLGMPGMHGSVAANRAISEADVLLGIGLRFDDRVTGRVNGFAPNAAIIHVEL
CCCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCCEEEEEEEE
DAAEIGKIVRTHVPVRGDAAIAARLLTEGAQRLDLPEWQAQLEAWKARTQKPDHWGAASA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHH
VQAVVERLRPDDILASDVGQHQMLAAQLARFERPRRWLNSGGLGTMGFGFPAAIGAALAE
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PGVRSVVIAGDGGFQMTAQELATLKMYDIRNVKICIINNSYLGMVRQWQELFHERRYSEV
CCCCEEEEECCCCCCHHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
WLGDSNPDFLKLADAYGVPGYRADCAEELPAAINAWLADPKSALLEIVVPHEHGVFPMVP
EECCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCCCCC
AGAALSEMIEQEPPRVPTPGIEKNAAAKEAQDA
CCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TQGEMTGAKALWATLVRHGINTVFGYPGGAIMPVYDALTFYPEVRHILTRHEQGAIHAA
CCCCCCHHHHHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECC
EGWAKATGEIGVCLATSGPGATNLVTGLADAMMDSVPILAITGNVARHLMGTDAFQEADI
CCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHCCCCHHCCCC
TGITLPITKHNYVVREVEELPRILAEAIRIARSGRPGPVLVDIPKDIQLAPYHGEIAAPH
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCCCC
ARPEAPAPAPEAIQRARELLRGAKKPVLLVGGGSLDAAAEITALARAWHLPVITTLMGLG
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AFPASDPLWLGMPGMHGSVAANRAISEADVLLGIGLRFDDRVTGRVNGFAPNAAIIHVEL
CCCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEECCCCCCEEEEEEEE
DAAEIGKIVRTHVPVRGDAAIAARLLTEGAQRLDLPEWQAQLEAWKARTQKPDHWGAASA
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHH
VQAVVERLRPDDILASDVGQHQMLAAQLARFERPRRWLNSGGLGTMGFGFPAAIGAALAE
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCC
PGVRSVVIAGDGGFQMTAQELATLKMYDIRNVKICIINNSYLGMVRQWQELFHERRYSEV
CCCCEEEEECCCCCCHHHHHHHHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCEE
WLGDSNPDFLKLADAYGVPGYRADCAEELPAAINAWLADPKSALLEIVVPHEHGVFPMVP
EECCCCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCCCCCCC
AGAALSEMIEQEPPRVPTPGIEKNAAAKEAQDA
CCHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]