Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 94984658

GI number: 94984658

Start: 585564

End: 586697

Strand: Reverse

Name: cca [H]

Synonym: Dgeo_0551

Alternate gene names: 94984658

Gene position: 586697-585564 (Counterclockwise)

Preceding gene: 94984659

Following gene: 94984657

Centisome position: 23.78

GC content: 72.31

Gene sequence:

>1134_bases
GTGGACAGCACGGCACAGGACCCCACCGGGCAGGCGTGGCAACGTCTTTTGGTCGCCGATCGCCGCTGGCTGACTGACCT
GGCACAGCGGGCGGGACCCGGCACGCGCCTGGCGCTGGTGGGCGGCGCAGTGCGCGACGCGCTGCTGGGCAAAACCCCCC
TCGACCTCGACGTGGTGGTGGAGGGAACAGACGCGGGCAAGCTGGCTGCAGCAACCGGCCTCCCCTTCCTGGTCCACCCG
GCCTTTGGCAACGCGACTGTGACCTTGCCCGACGGGCGGCACGTCGACCTGGTGCGAGCCCGTCGGGAAACGTATCCGGT
ACCCGGCGGCAATCCGGTCCCGGCGCCCGGCACCCTGGCGGACGACCTGCAGCGGCGCGACTTCGGGCTGAACGCGCTGG
CATTGCCCATCGGTCCAGACGGCCCCGGTCCGCTCCTTGATGAGGTCAACGGCCTGGACGATCTGCGGAAGCGCGCGCTG
CGGCCTCTGCACCCCCGCTCGCTGTACGAAGATGCCAGCCGCCTGGTCCGCGGGGCACGGCTGGCCGGACGCCTGGACCT
GAGCGCACACCCTGAGCTGCTGCGGCAGGTCCCCGACGCGCTGGAGCAGGCAGACACCACACCCCGCCTGCGGGCCGAGC
TGCGCCTGCTGCTGCACGAGCCGCGGCCGGGACGTGCCGCTCGCATGCTGGAGGCGTGGGGGGCCGCAAGCCTGCTGCCC
CCAGGGACCGCAACACGGCTGGAGGCACTCGATGCCCAGCAGGACGCAGGACAACCGGTACCCGCTCAGGCCTATGCCGC
TGCCCTTCTCTCCGCCGCCCCCGACCCCACCGCGCTGGCCGCTCGCCTGGGGCTGGGGGAGAAACCGGGGGGACTGCTGG
CCCGTGCCCTCTCGGATACCCCCACCTCACCTGGCAGCGCAGAAGCTGTCCTGCGTTCGCTGCTGCGGCCAGACGCCTAT
CCGCCCCTCAGCGGGCGGGATGTGCTGGCGCTGGGGGTCCCACCCGGCCCAGCGGTGGGCCAGGCCCTCGCGCATCTGGC
CACGCTGCGACGTGCCGGACAGGTTCATTCCCCCGAGGAGGAAAGGGCAGCGCTGAGGGCCTATTTGCAAGATGCGGCCA
CCGCCCAGAGGTAA

Upstream 100 bases:

>100_bases
TGACAAGCAGCCGGGGGATAGCGTCACGCTGCGCGTTGTGCGGGGTAAGCAGACGCGGGACGTGAAGGTCACCCTGGACA
ACAGCTCCTTCGAGTAAGCC

Downstream 100 bases:

>100_bases
GGGGGCCTCTGGTCCCGGAGGCGGGCCGTAGAATGCACCGATATGCTGCTCAGTCTGCTCTCCAGGGATCCCGTCGCATT
CATGATCATTGCGGCGGCGC

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDSTAQDPTGQAWQRLLVADRRWLTDLAQRAGPGTRLALVGGAVRDALLGKTPLDLDVVVEGTDAGKLAAATGLPFLVHP
AFGNATVTLPDGRHVDLVRARRETYPVPGGNPVPAPGTLADDLQRRDFGLNALALPIGPDGPGPLLDEVNGLDDLRKRAL
RPLHPRSLYEDASRLVRGARLAGRLDLSAHPELLRQVPDALEQADTTPRLRAELRLLLHEPRPGRAARMLEAWGAASLLP
PGTATRLEALDAQQDAGQPVPAQAYAAALLSAAPDPTALAARLGLGEKPGGLLARALSDTPTSPGSAEAVLRSLLRPDAY
PPLSGRDVLALGVPPGPAVGQALAHLATLRRAGQVHSPEEERAALRAYLQDAATAQR

Sequences:

>Translated_377_residues
MDSTAQDPTGQAWQRLLVADRRWLTDLAQRAGPGTRLALVGGAVRDALLGKTPLDLDVVVEGTDAGKLAAATGLPFLVHP
AFGNATVTLPDGRHVDLVRARRETYPVPGGNPVPAPGTLADDLQRRDFGLNALALPIGPDGPGPLLDEVNGLDDLRKRAL
RPLHPRSLYEDASRLVRGARLAGRLDLSAHPELLRQVPDALEQADTTPRLRAELRLLLHEPRPGRAARMLEAWGAASLLP
PGTATRLEALDAQQDAGQPVPAQAYAAALLSAAPDPTALAARLGLGEKPGGLLARALSDTPTSPGSAEAVLRSLLRPDAY
PPLSGRDVLALGVPPGPAVGQALAHLATLRRAGQVHSPEEERAALRAYLQDAATAQR
>Mature_377_residues
MDSTAQDPTGQAWQRLLVADRRWLTDLAQRAGPGTRLALVGGAVRDALLGKTPLDLDVVVEGTDAGKLAAATGLPFLVHP
AFGNATVTLPDGRHVDLVRARRETYPVPGGNPVPAPGTLADDLQRRDFGLNALALPIGPDGPGPLLDEVNGLDDLRKRAL
RPLHPRSLYEDASRLVRGARLAGRLDLSAHPELLRQVPDALEQADTTPRLRAELRLLLHEPRPGRAARMLEAWGAASLLP
PGTATRLEALDAQQDAGQPVPAQAYAAALLSAAPDPTALAARLGLGEKPGGLLARALSDTPTSPGSAEAVLRSLLRPDAY
PPLSGRDVLALGVPPGPAVGQALAHLATLRRAGQVHSPEEERAALRAYLQDAATAQR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=316, Percent_Identity=31.9620253164557, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081691, Length=179, Percent_Identity=32.9608938547486, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 39599; Mature: 39599

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSTAQDPTGQAWQRLLVADRRWLTDLAQRAGPGTRLALVGGAVRDALLGKTPLDLDVVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCEEEEE
EGTDAGKLAAATGLPFLVHPAFGNATVTLPDGRHVDLVRARRETYPVPGGNPVPAPGTLA
ECCCCCCHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHH
DDLQRRDFGLNALALPIGPDGPGPLLDEVNGLDDLRKRALRPLHPRSLYEDASRLVRGAR
HHHHHHHCCCCEEEEECCCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LAGRLDLSAHPELLRQVPDALEQADTTPRLRAELRLLLHEPRPGRAARMLEAWGAASLLP
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
PGTATRLEALDAQQDAGQPVPAQAYAAALLSAAPDPTALAARLGLGEKPGGLLARALSDT
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
PTSPGSAEAVLRSLLRPDAYPPLSGRDVLALGVPPGPAVGQALAHLATLRRAGQVHSPEE
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHH
ERAALRAYLQDAATAQR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSTAQDPTGQAWQRLLVADRRWLTDLAQRAGPGTRLALVGGAVRDALLGKTPLDLDVVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCEEEEE
EGTDAGKLAAATGLPFLVHPAFGNATVTLPDGRHVDLVRARRETYPVPGGNPVPAPGTLA
ECCCCCCHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHH
DDLQRRDFGLNALALPIGPDGPGPLLDEVNGLDDLRKRALRPLHPRSLYEDASRLVRGAR
HHHHHHHCCCCEEEEECCCCCCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LAGRLDLSAHPELLRQVPDALEQADTTPRLRAELRLLLHEPRPGRAARMLEAWGAASLLP
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
PGTATRLEALDAQQDAGQPVPAQAYAAALLSAAPDPTALAARLGLGEKPGGLLARALSDT
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
PTSPGSAEAVLRSLLRPDAYPPLSGRDVLALGVPPGPAVGQALAHLATLRRAGQVHSPEE
CCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHH
ERAALRAYLQDAATAQR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA