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The map label for this gene is gyrB [H]

Identifier: 94984653

GI number: 94984653

Start: 578107

End: 580242

Strand: Reverse

Name: gyrB [H]

Synonym: Dgeo_0546

Alternate gene names: 94984653

Gene position: 580242-578107 (Counterclockwise)

Preceding gene: 94984657

Following gene: 94984650

Centisome position: 23.52

GC content: 61.1

Gene sequence:

>2136_bases
GTGTCCACCTGTTATTACGCTCATAACGTAATCAAGTTGACCCATTTCAGTCTAAGTTGTATACTAAGAAAACTTCGGAG
GCGCACCCAGCGCCCTTTTTTCAGCAGCGCAGTCTCCGAAATCGTGCAAGATCACCCCCATTGGGGACGACGCAGGAGGA
CCATGACGCAAGCTCTCCCCCAAGACCACTACACGGCTGATTCCATCTCGATTCTCAAGGGGCTGGAAGCGGTGCGCAAA
CGCCCCGGCATGTACGTGCAGGGCGGCACCGGAATCGACGGCTACCACCAGCTCCTGACCGAGATTATTGACAACGCCAT
CGACGAGGGCCTGGCCGGGTTCGCCACCGAGGTGCACGTCATCATGCACGCCGACGGCTCGGCCACCGTCACCGACAACG
GACGCGGCATTCCCGTGGATATCATGAAGTCGGAAGGGCGCCCAGCCATTGAGGTTATCTTCACCGAGCTGCACGCGGGC
GGCAAGTTCGGCGGCGGTGCCTATAAGGTGTCGGGCGGCCTGCACGGGGTCGGCTCCAGCGTGGTGAACGCGCTGTCCAC
CTATCTCGACGTCACCGTCAACAAGGACGGCAACCTGCACCACATCCGCTTCGAGAGGGGGACCGTGACCACACCGCTCG
AAGTCCTGGGGCCAACGCCGCAGGACGTGCGGTGGGCCACCAAGGTCACCTTCCACCCCGACCCTACCGTCTTCAGCGAG
TTCGAGAACCTCTTCAACTACGACCGCATTCGCGGCCGCTTGCGCGAGCTGGCCTACCTGACTGGCCTCAAAATCGTGGT
CCGTGACGAGCGCGTCGGGTTGCACGGCGGCGAAATTCGCGAGGAGACCTTTCATGAGCAGGGCGGCATCGCCAACTTCG
CCCGCGCGCTCGTCACCGATGACTCCAAGCTGCTCTATGACCAGCCCATCGTGATGCGCGGCACCCACAGTGACGTGGAG
GTCGAGGTCGCCTTCATTCATGCCAACACCTACGCCAGCGACAACATCCTCACGTACGCGAACATGATTCGCACGCGCGA
CGGCGGCACGCCCCTCACCGGCTTCAAAACCGCCTATACCCGCATCCTGAATAAGTACGCCAAAGACAAAAATCTGATCA
AGTCGGGCAATCCGGTCCCCACCGGCGATGACCTGCTGGAAGGCATCTACTGCGTGGTGTCGGTCAAGCTGCCCGAGCCG
CAGTTCGAGTCGCAGGCCAAGGTCAAGCTGCTCAACAGCGAGGCACAGACCGCCGTGAACGCCATTGTGGGCGAGAAGTT
CACGGAGTTTCTGGAGGAAAATCCCAAGATCGGCCGGACCATTGTGGAAAAGGCAGCTGAGGCGGCCCGCGCCCGCGAGG
CGGCCCGCAAGGCCCGCGACATCGTGCGCCGCTCCAATCCGCTGGAGAACGACGACCTGCCCGGCAAGCTGGCCGACTGC
ACCAGCCAGGACCCAGCGGAATCCGAACTCTTCATCGTGGAAGGGGACTCGGCGGGCGGCAGCGCCAAGATGGGCCGGGA
GCGCCGCTATCAGGCCATTCTTCCGCTGCGCGGCAAGATTCTGAATGTCGAAAAGTCCGACCTGAACAAGATTCTGAAAA
ACGCCGAGATTCGCGCGATGATCGGGGCCATCGGGGCGGGCGTCGAGGGCACCGGGGACCGAATGCACTTCGACCTTTCC
AACCTGCGCTACCACAAGATCATCATCATGACGGACGCGGACATGGACGGCGGCCACATCACCACGCTGCTGCTGACGTT
CTTCTACCGCTATATGCGCCCCATCATCGAAGCCGGGTATCTCTATATCGCGCAGCCACCGCTCTACCGCATCATGGTGG
GCCGCGAGAAAAAAGGCACCTACCTCTACACCGAAGAGGAACTCAAGGAACACGTCGCGCGCGCCACAAAGGAAGGCAAG
AAGTACGAGATCCAGCGCTTCAAGGGGCTGGGCGAGATGAACGCCGAGCAGCTCTGGGACACCACCATGAACCCCGAGAC
ACGCGCGCTGAAGCGCGTGCAGGTAGAAGACCTGATCATCGCCAACGAGGTCTTCGAAGACCTGATGGGGAATGAAGTGG
GGCCGCGCAAGCGGTTTATTCAGGAAAATGCGCGTTTTGCGGAAATCAGCATCTGA

Upstream 100 bases:

>100_bases
CCATTCTGCCCGCAGAGCGCAGGCGGCGTGAAGGGCATCACCGGTTCAGGCACAAGGAGGGCGCGCAGACCGCCTCTTCA
GGGCGCACCGGGGAGGACAA

Downstream 100 bases:

>100_bases
GTGAAGACACCAAGAAGCCGCCTGCCCGTGGGGTGGGCGGTTTTTCTTTCACAAGGCGGCTTCATCCCGGGAGAGCGCTG
CACTGGCTGTCTGCAGGCAG

Product: DNA topoisomerase (ATP-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MSTCYYAHNVIKLTHFSLSCILRKLRRRTQRPFFSSAVSEIVQDHPHWGRRRRTMTQALPQDHYTADSISILKGLEAVRK
RPGMYVQGGTGIDGYHQLLTEIIDNAIDEGLAGFATEVHVIMHADGSATVTDNGRGIPVDIMKSEGRPAIEVIFTELHAG
GKFGGGAYKVSGGLHGVGSSVVNALSTYLDVTVNKDGNLHHIRFERGTVTTPLEVLGPTPQDVRWATKVTFHPDPTVFSE
FENLFNYDRIRGRLRELAYLTGLKIVVRDERVGLHGGEIREETFHEQGGIANFARALVTDDSKLLYDQPIVMRGTHSDVE
VEVAFIHANTYASDNILTYANMIRTRDGGTPLTGFKTAYTRILNKYAKDKNLIKSGNPVPTGDDLLEGIYCVVSVKLPEP
QFESQAKVKLLNSEAQTAVNAIVGEKFTEFLEENPKIGRTIVEKAAEAARAREAARKARDIVRRSNPLENDDLPGKLADC
TSQDPAESELFIVEGDSAGGSAKMGRERRYQAILPLRGKILNVEKSDLNKILKNAEIRAMIGAIGAGVEGTGDRMHFDLS
NLRYHKIIIMTDADMDGGHITTLLLTFFYRYMRPIIEAGYLYIAQPPLYRIMVGREKKGTYLYTEEELKEHVARATKEGK
KYEIQRFKGLGEMNAEQLWDTTMNPETRALKRVQVEDLIIANEVFEDLMGNEVGPRKRFIQENARFAEISI

Sequences:

>Translated_711_residues
MSTCYYAHNVIKLTHFSLSCILRKLRRRTQRPFFSSAVSEIVQDHPHWGRRRRTMTQALPQDHYTADSISILKGLEAVRK
RPGMYVQGGTGIDGYHQLLTEIIDNAIDEGLAGFATEVHVIMHADGSATVTDNGRGIPVDIMKSEGRPAIEVIFTELHAG
GKFGGGAYKVSGGLHGVGSSVVNALSTYLDVTVNKDGNLHHIRFERGTVTTPLEVLGPTPQDVRWATKVTFHPDPTVFSE
FENLFNYDRIRGRLRELAYLTGLKIVVRDERVGLHGGEIREETFHEQGGIANFARALVTDDSKLLYDQPIVMRGTHSDVE
VEVAFIHANTYASDNILTYANMIRTRDGGTPLTGFKTAYTRILNKYAKDKNLIKSGNPVPTGDDLLEGIYCVVSVKLPEP
QFESQAKVKLLNSEAQTAVNAIVGEKFTEFLEENPKIGRTIVEKAAEAARAREAARKARDIVRRSNPLENDDLPGKLADC
TSQDPAESELFIVEGDSAGGSAKMGRERRYQAILPLRGKILNVEKSDLNKILKNAEIRAMIGAIGAGVEGTGDRMHFDLS
NLRYHKIIIMTDADMDGGHITTLLLTFFYRYMRPIIEAGYLYIAQPPLYRIMVGREKKGTYLYTEEELKEHVARATKEGK
KYEIQRFKGLGEMNAEQLWDTTMNPETRALKRVQVEDLIIANEVFEDLMGNEVGPRKRFIQENARFAEISI
>Mature_710_residues
STCYYAHNVIKLTHFSLSCILRKLRRRTQRPFFSSAVSEIVQDHPHWGRRRRTMTQALPQDHYTADSISILKGLEAVRKR
PGMYVQGGTGIDGYHQLLTEIIDNAIDEGLAGFATEVHVIMHADGSATVTDNGRGIPVDIMKSEGRPAIEVIFTELHAGG
KFGGGAYKVSGGLHGVGSSVVNALSTYLDVTVNKDGNLHHIRFERGTVTTPLEVLGPTPQDVRWATKVTFHPDPTVFSEF
ENLFNYDRIRGRLRELAYLTGLKIVVRDERVGLHGGEIREETFHEQGGIANFARALVTDDSKLLYDQPIVMRGTHSDVEV
EVAFIHANTYASDNILTYANMIRTRDGGTPLTGFKTAYTRILNKYAKDKNLIKSGNPVPTGDDLLEGIYCVVSVKLPEPQ
FESQAKVKLLNSEAQTAVNAIVGEKFTEFLEENPKIGRTIVEKAAEAARAREAARKARDIVRRSNPLENDDLPGKLADCT
SQDPAESELFIVEGDSAGGSAKMGRERRYQAILPLRGKILNVEKSDLNKILKNAEIRAMIGAIGAGVEGTGDRMHFDLSN
LRYHKIIIMTDADMDGGHITTLLLTFFYRYMRPIIEAGYLYIAQPPLYRIMVGREKKGTYLYTEEELKEHVARATKEGKK
YEIQRFKGLGEMNAEQLWDTTMNPETRALKRVQVEDLIIANEVFEDLMGNEVGPRKRFIQENARFAEISI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=594, Percent_Identity=24.5791245791246, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI19913406, Length=586, Percent_Identity=23.7201365187713, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI48994957, Length=572, Percent_Identity=47.9020979020979, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1789408, Length=664, Percent_Identity=39.0060240963855, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI212645845, Length=541, Percent_Identity=26.2476894639556, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17535065, Length=580, Percent_Identity=24.1379310344828, Blast_Score=116, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=187, Percent_Identity=27.807486631016, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=651, Percent_Identity=26.2672811059908, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136538, Length=592, Percent_Identity=24.4932432432432, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 79458; Mature: 79326

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTCYYAHNVIKLTHFSLSCILRKLRRRTQRPFFSSAVSEIVQDHPHWGRRRRTMTQALP
CCCEEECCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCC
QDHYTADSISILKGLEAVRKRPGMYVQGGTGIDGYHQLLTEIIDNAIDEGLAGFATEVHV
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEE
IMHADGSATVTDNGRGIPVDIMKSEGRPAIEVIFTELHAGGKFGGGAYKVSGGLHGVGSS
EEECCCCEEECCCCCCCEEEEECCCCCCCHHHEEEHHHCCCCCCCCEEEECCCCCCCHHH
VVNALSTYLDVTVNKDGNLHHIRFERGTVTTPLEVLGPTPQDVRWATKVTFHPDPTVFSE
HHHHHHHHEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCCCCEEEEEEEECCCCHHHHH
FENLFNYDRIRGRLRELAYLTGLKIVVRDERVGLHGGEIREETFHEQGGIANFARALVTD
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCC
DSKLLYDQPIVMRGTHSDVEVEVAFIHANTYASDNILTYANMIRTRDGGTPLTGFKTAYT
CCCEEECCCEEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
RILNKYAKDKNLIKSGNPVPTGDDLLEGIYCVVSVKLPEPQFESQAKVKLLNSEAQTAVN
HHHHHHHHCCHHHCCCCCCCCHHHHHHCEEEEEEEECCCCCCCCCCHHEEECHHHHHHHH
AIVGEKFTEFLEENPKIGRTIVEKAAEAARAREAARKARDIVRRSNPLENDDLPGKLADC
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
TSQDPAESELFIVEGDSAGGSAKMGRERRYQAILPLRGKILNVEKSDLNKILKNAEIRAM
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEECCHHHHHHHHHCCHHHHH
IGAIGAGVEGTGDRMHFDLSNLRYHKIIIMTDADMDGGHITTLLLTFFYRYMRPIIEAGY
HHHHCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCE
LYIAQPPLYRIMVGREKKGTYLYTEEELKEHVARATKEGKKYEIQRFKGLGEMNAEQLWD
EEEECCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHC
TTMNPETRALKRVQVEDLIIANEVFEDLMGNEVGPRKRFIQENARFAEISI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
STCYYAHNVIKLTHFSLSCILRKLRRRTQRPFFSSAVSEIVQDHPHWGRRRRTMTQALP
CCEEECCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCC
QDHYTADSISILKGLEAVRKRPGMYVQGGTGIDGYHQLLTEIIDNAIDEGLAGFATEVHV
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCEEEEE
IMHADGSATVTDNGRGIPVDIMKSEGRPAIEVIFTELHAGGKFGGGAYKVSGGLHGVGSS
EEECCCCEEECCCCCCCEEEEECCCCCCCHHHEEEHHHCCCCCCCCEEEECCCCCCCHHH
VVNALSTYLDVTVNKDGNLHHIRFERGTVTTPLEVLGPTPQDVRWATKVTFHPDPTVFSE
HHHHHHHHEEEEECCCCCEEEEEEECCCCCCCHHHCCCCCCCCEEEEEEEECCCCHHHHH
FENLFNYDRIRGRLRELAYLTGLKIVVRDERVGLHGGEIREETFHEQGGIANFARALVTD
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCC
DSKLLYDQPIVMRGTHSDVEVEVAFIHANTYASDNILTYANMIRTRDGGTPLTGFKTAYT
CCCEEECCCEEEECCCCCEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
RILNKYAKDKNLIKSGNPVPTGDDLLEGIYCVVSVKLPEPQFESQAKVKLLNSEAQTAVN
HHHHHHHHCCHHHCCCCCCCCHHHHHHCEEEEEEEECCCCCCCCCCHHEEECHHHHHHHH
AIVGEKFTEFLEENPKIGRTIVEKAAEAARAREAARKARDIVRRSNPLENDDLPGKLADC
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCC
TSQDPAESELFIVEGDSAGGSAKMGRERRYQAILPLRGKILNVEKSDLNKILKNAEIRAM
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHEECCCCCEEEECCHHHHHHHHHCCHHHHH
IGAIGAGVEGTGDRMHFDLSNLRYHKIIIMTDADMDGGHITTLLLTFFYRYMRPIIEAGY
HHHHCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCE
LYIAQPPLYRIMVGREKKGTYLYTEEELKEHVARATKEGKKYEIQRFKGLGEMNAEQLWD
EEEECCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHC
TTMNPETRALKRVQVEDLIIANEVFEDLMGNEVGPRKRFIQENARFAEISI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]