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The map label for this gene is glgX [H]

Identifier: 94984648

GI number: 94984648

Start: 570089

End: 572272

Strand: Reverse

Name: glgX [H]

Synonym: Dgeo_0541

Alternate gene names: 94984648

Gene position: 572272-570089 (Counterclockwise)

Preceding gene: 94984649

Following gene: 94984647

Centisome position: 23.2

GC content: 63.42

Gene sequence:

>2184_bases
ATGACGACCACCGAACCCGCGCTTCTTCCGCTTGCGGCCAACGCCGCGAGTTCCCGCGTTCGCCCGGGTCAGCCCTATCC
GCTGGGCGCGACCTGGGACGGCAAGGGAACCAACTTTGCCCTGTATTCCGAGAACGCGACTGCCGTCGAGCTCTGCCTGT
TCGACGAAAACGGCCAGGAAACACGCTATCCGCTCACCGAGCAGACCGCCTTTGTGTGGCACGGGTACCTGCCGGCCATC
GGGCCGGGCCAGCGCTACGGCTACCGCGTCTACGGCGAATACGCCCCCGAAAAAGGGCTGCGCTTCAACCCCAACGTGGT
GCTGCTCGATCCCTACGCCAAGGCGCTGGACGGCACCGAACGCTTTGACAAAGGCGTGTTCGGCTATGTGGTCGGCCAAG
AGGACACCGTGATGCAGCCGGAAGAGCAGCGCGGCGCTCCACTGGGCATCGTGATCGACCCGGGGTTTAACTGGGTGGGC
GATGTCAAGCCCAACGTGCCCTTTCACCAATCGGTGATCTACGAGACGCACGTCAAAGGCCTGACCATGACGCACCCTGA
GGTGCCCGAAGCGCTGCGCGGCACCTACGCGGGCGTGGCGACCGAACCGGTGCTCCGGTATCTCAGGGAGCTGGGCATCA
CGGCGGTTGAGTTTCTGCCGGTGCATCAGCACCTCGACGATCCCTTTTTGCTGGAAAAGGGCCTCACCAACTACTGGGGC
TACTCCACCCTCAGTTTCTTTGCGCCGGACGTGCGCTACTCGGCGGCGGCGCGGCGGGGTGACCCAGCCGGCGCTGTGCC
TGAGTTCAAGAACATGGTGCGCGCCCTGCACGACGCGGGCATCGAGGTGATTCTCGACGTGGTGTACAACCACACCGCAG
AAGGCAATCATCTGGGGCCGACCCTCTCTTTCAAGGGCATCGACAACCCCACCTACTACCGCCTGGTGGCCGACAACCCG
CGCTTCTACTTCGACTACACCGGCACCGGCAACAGCCTCAACGTTCGGCACCCGCAGACCCTCCAGCTCATCATGGACAG
CCTGCGTTACTGGGTGACCGACATGCATGTGGACGGTTTCCGTTTCGACCTGGCCTCAACGCTGGCGCGTGGGTTGCACG
AGGTGGATCAGCTTTCCTCCTTCTTCACGATCATTCATCAGGACCCGGTGCTGAGCCGGGTCAAGCTGATTGCTGAACCC
TGGGACGTGGGTGAGGGCGGCTATCAGGTGGGCAACTTCCCGGTGAAGTGGGCGGAGTGGAACGGTCTGTACCGCGACGC
CATGCGCGCTTTCTGGAAGGGCGAGGGCGGCCTGGCCAGTGAAATCGGCTACCGCCTGACGGGCTCCAGCGACCTCTATC
AGAACGACGGGCGCAAGCCCTATGCCTCGATCAACTTCGTGACTGCGCACGACGGCTTCACGCTGCGCGACGCGGTGACC
TACGAACAAAAGCACAACGAGGCCAATGGTGAGGGCAACCAGGATGGCCACAACCACAACCTCACCTGGAACTGCGGCGT
GGAGGGCGAGACGGACGATCCTGAGGTCAACCGCCTGCGACACCAGCAGCAGCGCAACCTGCTGGCCACACTGCTGCTGA
GCCAGGGCACGCCCATGCTGCTCGGCGGCGACGAAATCGGCCGGACCCAAAAGGGCAACAACAACGCCTACTGTCAGGAC
AATGAGATCAGCTGGTACGACTGGGCGAACATCGACACCGACCTGCTGGCCTTTACCCGCAAGCTGATCGCGCTGCGCAA
GACGCATCCCGCGTTGCATCGCCGCAAGTTCTTCAGCGGGCGCACCATTCGCGGGGAGGACGTACGCGACATCGTGTGGC
TGCGCTTCGACGGCCAGGAGTTGAGCGACGAGGATTGGAATAACCCCCAAACCCAGTCGCTGGGGATGTTCCTCGATGGT
GACGGCCTAGACGACGTGGACGCCGAGGGGAATCCCTTGCGGGACGACGACCTGCTGTTGCTGCTCAGCGCCTCACACGT
GGATCTGCCCTTCCGCTTGCCGGCCACCGGCGGCTGCCAAACCTGGGAACTGCTGCTCGACACAACCAACGACCAGGCCC
AAGAAACCGTGGAAGCCGGGCAAGAAACCACCCTCAAAGCCCGGAGCGTCAAACTCTACCGCTGCGTTCAGCATGCGGAA
GGGGCGGCGTCCGAAGACCTGTAG

Upstream 100 bases:

>100_bases
GTAACGTTTCCAACTGTGGGCGGCGACTTCACGGGTTGACCGGGAGCCGCTGTTTTTTGATCCTCACTTCAGCAACCGGG
CTGCTGCCCGTGGAGGCCCT

Downstream 100 bases:

>100_bases
GCGGCCCAAAGGGAGACCACGCCGGAGGGCAACCAGCCGAAGGGTTGCCCTTTTTGCCTTATGCTCAGGGTTCGCTCACT
TAAAAACGAGTCCTTCTTCA

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MTTTEPALLPLAANAASSRVRPGQPYPLGATWDGKGTNFALYSENATAVELCLFDENGQETRYPLTEQTAFVWHGYLPAI
GPGQRYGYRVYGEYAPEKGLRFNPNVVLLDPYAKALDGTERFDKGVFGYVVGQEDTVMQPEEQRGAPLGIVIDPGFNWVG
DVKPNVPFHQSVIYETHVKGLTMTHPEVPEALRGTYAGVATEPVLRYLRELGITAVEFLPVHQHLDDPFLLEKGLTNYWG
YSTLSFFAPDVRYSAAARRGDPAGAVPEFKNMVRALHDAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNPTYYRLVADNP
RFYFDYTGTGNSLNVRHPQTLQLIMDSLRYWVTDMHVDGFRFDLASTLARGLHEVDQLSSFFTIIHQDPVLSRVKLIAEP
WDVGEGGYQVGNFPVKWAEWNGLYRDAMRAFWKGEGGLASEIGYRLTGSSDLYQNDGRKPYASINFVTAHDGFTLRDAVT
YEQKHNEANGEGNQDGHNHNLTWNCGVEGETDDPEVNRLRHQQQRNLLATLLLSQGTPMLLGGDEIGRTQKGNNNAYCQD
NEISWYDWANIDTDLLAFTRKLIALRKTHPALHRRKFFSGRTIRGEDVRDIVWLRFDGQELSDEDWNNPQTQSLGMFLDG
DGLDDVDAEGNPLRDDDLLLLLSASHVDLPFRLPATGGCQTWELLLDTTNDQAQETVEAGQETTLKARSVKLYRCVQHAE
GAASEDL

Sequences:

>Translated_727_residues
MTTTEPALLPLAANAASSRVRPGQPYPLGATWDGKGTNFALYSENATAVELCLFDENGQETRYPLTEQTAFVWHGYLPAI
GPGQRYGYRVYGEYAPEKGLRFNPNVVLLDPYAKALDGTERFDKGVFGYVVGQEDTVMQPEEQRGAPLGIVIDPGFNWVG
DVKPNVPFHQSVIYETHVKGLTMTHPEVPEALRGTYAGVATEPVLRYLRELGITAVEFLPVHQHLDDPFLLEKGLTNYWG
YSTLSFFAPDVRYSAAARRGDPAGAVPEFKNMVRALHDAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNPTYYRLVADNP
RFYFDYTGTGNSLNVRHPQTLQLIMDSLRYWVTDMHVDGFRFDLASTLARGLHEVDQLSSFFTIIHQDPVLSRVKLIAEP
WDVGEGGYQVGNFPVKWAEWNGLYRDAMRAFWKGEGGLASEIGYRLTGSSDLYQNDGRKPYASINFVTAHDGFTLRDAVT
YEQKHNEANGEGNQDGHNHNLTWNCGVEGETDDPEVNRLRHQQQRNLLATLLLSQGTPMLLGGDEIGRTQKGNNNAYCQD
NEISWYDWANIDTDLLAFTRKLIALRKTHPALHRRKFFSGRTIRGEDVRDIVWLRFDGQELSDEDWNNPQTQSLGMFLDG
DGLDDVDAEGNPLRDDDLLLLLSASHVDLPFRLPATGGCQTWELLLDTTNDQAQETVEAGQETTLKARSVKLYRCVQHAE
GAASEDL
>Mature_726_residues
TTTEPALLPLAANAASSRVRPGQPYPLGATWDGKGTNFALYSENATAVELCLFDENGQETRYPLTEQTAFVWHGYLPAIG
PGQRYGYRVYGEYAPEKGLRFNPNVVLLDPYAKALDGTERFDKGVFGYVVGQEDTVMQPEEQRGAPLGIVIDPGFNWVGD
VKPNVPFHQSVIYETHVKGLTMTHPEVPEALRGTYAGVATEPVLRYLRELGITAVEFLPVHQHLDDPFLLEKGLTNYWGY
STLSFFAPDVRYSAAARRGDPAGAVPEFKNMVRALHDAGIEVILDVVYNHTAEGNHLGPTLSFKGIDNPTYYRLVADNPR
FYFDYTGTGNSLNVRHPQTLQLIMDSLRYWVTDMHVDGFRFDLASTLARGLHEVDQLSSFFTIIHQDPVLSRVKLIAEPW
DVGEGGYQVGNFPVKWAEWNGLYRDAMRAFWKGEGGLASEIGYRLTGSSDLYQNDGRKPYASINFVTAHDGFTLRDAVTY
EQKHNEANGEGNQDGHNHNLTWNCGVEGETDDPEVNRLRHQQQRNLLATLLLSQGTPMLLGGDEIGRTQKGNNNAYCQDN
EISWYDWANIDTDLLAFTRKLIALRKTHPALHRRKFFSGRTIRGEDVRDIVWLRFDGQELSDEDWNNPQTQSLGMFLDGD
GLDDVDAEGNPLRDDDLLLLLSASHVDLPFRLPATGGCQTWELLLDTTNDQAQETVEAGQETTLKARSVKLYRCVQHAEG
AASEDL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=610, Percent_Identity=47.5409836065574, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 81356; Mature: 81225

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTEPALLPLAANAASSRVRPGQPYPLGATWDGKGTNFALYSENATAVELCLFDENGQE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCC
TRYPLTEQTAFVWHGYLPAIGPGQRYGYRVYGEYAPEKGLRFNPNVVLLDPYAKALDGTE
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEECCCEEEECCHHHHCCCHH
RFDKGVFGYVVGQEDTVMQPEEQRGAPLGIVIDPGFNWVGDVKPNVPFHQSVIYETHVKG
HHCCCCEEEEECCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHEEEHHCCC
LTMTHPEVPEALRGTYAGVATEPVLRYLRELGITAVEFLPVHQHLDDPFLLEKGLTNYWG
EEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEHHHCCCCCHHHHHCCCHHCC
YSTLSFFAPDVRYSAAARRGDPAGAVPEFKNMVRALHDAGIEVILDVVYNHTAEGNHLGP
CCHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
TLSFKGIDNPTYYRLVADNPRFYFDYTGTGNSLNVRHPQTLQLIMDSLRYWVTDMHVDGF
EEEECCCCCCEEEEEEECCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHEECCCC
RFDLASTLARGLHEVDQLSSFFTIIHQDPVLSRVKLIAEPWDVGEGGYQVGNFPVKWAEW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEECCC
NGLYRDAMRAFWKGEGGLASEIGYRLTGSSDLYQNDGRKPYASINFVTAHDGFTLRDAVT
CCHHHHHHHHHHCCCCCCHHHCCEEECCCCHHHHCCCCCCEEEEEEEEECCCEEEHHHHH
YEQKHNEANGEGNQDGHNHNLTWNCGVEGETDDPEVNRLRHQQQRNLLATLLLSQGTPML
HHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
LGGDEIGRTQKGNNNAYCQDNEISWYDWANIDTDLLAFTRKLIALRKTHPALHRRKFFSG
ECCHHHCCCCCCCCCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
RTIRGEDVRDIVWLRFDGQELSDEDWNNPQTQSLGMFLDGDGLDDVDAEGNPLRDDDLLL
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEE
LLSASHVDLPFRLPATGGCQTWELLLDTTNDQAQETVEAGQETTLKARSVKLYRCVQHAE
EEECCCCCCCEECCCCCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GAASEDL
CCCCCCC
>Mature Secondary Structure 
TTTEPALLPLAANAASSRVRPGQPYPLGATWDGKGTNFALYSENATAVELCLFDENGQE
CCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCC
TRYPLTEQTAFVWHGYLPAIGPGQRYGYRVYGEYAPEKGLRFNPNVVLLDPYAKALDGTE
CCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEECCCEEEECCHHHHCCCHH
RFDKGVFGYVVGQEDTVMQPEEQRGAPLGIVIDPGFNWVGDVKPNVPFHQSVIYETHVKG
HHCCCCEEEEECCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHEEEHHCCC
LTMTHPEVPEALRGTYAGVATEPVLRYLRELGITAVEFLPVHQHLDDPFLLEKGLTNYWG
EEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEHHHCCCCCHHHHHCCCHHCC
YSTLSFFAPDVRYSAAARRGDPAGAVPEFKNMVRALHDAGIEVILDVVYNHTAEGNHLGP
CCHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCC
TLSFKGIDNPTYYRLVADNPRFYFDYTGTGNSLNVRHPQTLQLIMDSLRYWVTDMHVDGF
EEEECCCCCCEEEEEEECCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHEECCCC
RFDLASTLARGLHEVDQLSSFFTIIHQDPVLSRVKLIAEPWDVGEGGYQVGNFPVKWAEW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEECCC
NGLYRDAMRAFWKGEGGLASEIGYRLTGSSDLYQNDGRKPYASINFVTAHDGFTLRDAVT
CCHHHHHHHHHHCCCCCCHHHCCEEECCCCHHHHCCCCCCEEEEEEEEECCCEEEHHHHH
YEQKHNEANGEGNQDGHNHNLTWNCGVEGETDDPEVNRLRHQQQRNLLATLLLSQGTPML
HHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
LGGDEIGRTQKGNNNAYCQDNEISWYDWANIDTDLLAFTRKLIALRKTHPALHRRKFFSG
ECCHHHCCCCCCCCCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCC
RTIRGEDVRDIVWLRFDGQELSDEDWNNPQTQSLGMFLDGDGLDDVDAEGNPLRDDDLLL
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEE
LLSASHVDLPFRLPATGGCQTWELLLDTTNDQAQETVEAGQETTLKARSVKLYRCVQHAE
EEECCCCCCCEECCCCCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GAASEDL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]