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The map label for this gene is pyrB

Identifier: 94984610

GI number: 94984610

Start: 522532

End: 523470

Strand: Reverse

Name: pyrB

Synonym: Dgeo_0503

Alternate gene names: 94984610

Gene position: 523470-522532 (Counterclockwise)

Preceding gene: 94984611

Following gene: 94984609

Centisome position: 21.22

GC content: 66.56

Gene sequence:

>939_bases
ATGACGGTCCTCGCGCCTCCCCGCCCCCGCAGCCTGCTGGACTTCCAAGACTGGCTGCCCGAGCGCCTCAGCGCCCTGCT
GGACAATGCCGACACCATGCACCAGGTGCTCGAGCGTCCGGTGAGGAAGGTCCCAGCGCTGCAAGGACTGACGGTCTGCA
CGGCCTTTTTCGAAAACAGCACCCGCACCCGCATCTCCTTTGAACTGGCCGCCCGGCGCATGAGTGCGGACGTCATTTCT
TTTGCGGCGGGTGCGAGCAGCCTCAGCAAGGGTGAATCTCTGCGCGACACCGTGGAAGTGCTCAGCGCCTACAAGGTGGA
TGCCTTCGTGGTGCGGCACCCAGCCAGTGGCGCCGCCCACCTCATCGCGCGATACAGCGGCAAGCCAGTGATCAATGCGG
GGGACGGGCGGCGAGCACACCCCACCCAAGCGCTGCTGGATGCCTACACCATCCGCCAGGAATACGGCAGCCTGGCCGGC
AAGAAGGTGGCCATCATCGGTGACATCCGCCATTCGCGGGTGGCCCGCAGCAACGCCGAACTCCTGCCGAAGCTGGGCGC
CGAGGTTGTGCTGGCTGGCCCCGCCACGCTGCTCCCCGCTGACCTCGCGGCGCTGCCCGGCGTGACGGTCACCACCGACC
CGAAAGAAGCGGTGCACGGCGCCCACGCAGTGATGGCCCTGCGCCTGCAGCAGGAGCGGATGAACGCGGGCTACCTCGCC
AGTTTGCAGGAGTACGTGGAGCGCTATCAGGTGAACGAGGCACTGCTGCGAGAAGCCGAAAGCGGCGCAATTGTCCTGCA
CCCAGGCCCCCTCAACCGTGACCTTGAAATCAGCTCCGAGGTGGCGGACGGCCCGCAGAGCCGCATCCTCAAGCAGGTCG
AGAACGGGCAGGCCGTGCGGATGAGCGTGCTGTATCACCTGCTGGTGGGACGAGACTGA

Upstream 100 bases:

>100_bases
GCAAGAACCTGCCCACGTCACGCAGCGAGATGGTCAAGGTGCGGCTCGCGGAGACGGACGGCGTGGACCTGGTGGAACTG
CACGATCTGGAGGAGCGGAG

Downstream 100 bases:

>100_bases
GGAAACGGCACTGAGGAAGCTCGCAGCCCAGTCAGCCATCAGCGGCAACGTTTGCGCCTCTGCAGGAAGCTGAAGGCTGC
TGGCCGAGCCTACTCACAGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTVLAPPRPRSLLDFQDWLPERLSALLDNADTMHQVLERPVRKVPALQGLTVCTAFFENSTRTRISFELAARRMSADVIS
FAAGASSLSKGESLRDTVEVLSAYKVDAFVVRHPASGAAHLIARYSGKPVINAGDGRRAHPTQALLDAYTIRQEYGSLAG
KKVAIIGDIRHSRVARSNAELLPKLGAEVVLAGPATLLPADLAALPGVTVTTDPKEAVHGAHAVMALRLQQERMNAGYLA
SLQEYVERYQVNEALLREAESGAIVLHPGPLNRDLEISSEVADGPQSRILKQVENGQAVRMSVLYHLLVGRD

Sequences:

>Translated_312_residues
MTVLAPPRPRSLLDFQDWLPERLSALLDNADTMHQVLERPVRKVPALQGLTVCTAFFENSTRTRISFELAARRMSADVIS
FAAGASSLSKGESLRDTVEVLSAYKVDAFVVRHPASGAAHLIARYSGKPVINAGDGRRAHPTQALLDAYTIRQEYGSLAG
KKVAIIGDIRHSRVARSNAELLPKLGAEVVLAGPATLLPADLAALPGVTVTTDPKEAVHGAHAVMALRLQQERMNAGYLA
SLQEYVERYQVNEALLREAESGAIVLHPGPLNRDLEISSEVADGPQSRILKQVENGQAVRMSVLYHLLVGRD
>Mature_311_residues
TVLAPPRPRSLLDFQDWLPERLSALLDNADTMHQVLERPVRKVPALQGLTVCTAFFENSTRTRISFELAARRMSADVISF
AAGASSLSKGESLRDTVEVLSAYKVDAFVVRHPASGAAHLIARYSGKPVINAGDGRRAHPTQALLDAYTIRQEYGSLAGK
KVAIIGDIRHSRVARSNAELLPKLGAEVVLAGPATLLPADLAALPGVTVTTDPKEAVHGAHAVMALRLQQERMNAGYLAS
LQEYVERYQVNEALLREAESGAIVLHPGPLNRDLEISSEVADGPQSRILKQVENGQAVRMSVLYHLLVGRD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=34.7402597402597, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI38788445, Length=318, Percent_Identity=27.6729559748428, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI2367364, Length=276, Percent_Identity=35.1449275362319, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786469, Length=336, Percent_Identity=25.297619047619, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790703, Length=336, Percent_Identity=25.297619047619, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=317, Percent_Identity=35.9621451104101, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=268, Percent_Identity=35.4477611940299, Blast_Score=149, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24642586, Length=289, Percent_Identity=35.6401384083045, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_DEIGD (Q1J129)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603974.1
- ProteinModelPortal:   Q1J129
- STRING:   Q1J129
- GeneID:   4057934
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0503
- NMPDR:   fig|68909.1.peg.804
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q1J129
- ProtClustDB:   PRK00856
- BioCyc:   DGEO319795:DGEO_0503-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33803; Mature: 33672

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLAPPRPRSLLDFQDWLPERLSALLDNADTMHQVLERPVRKVPALQGLTVCTAFFENS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
TRTRISFELAARRMSADVISFAAGASSLSKGESLRDTVEVLSAYKVDAFVVRHPASGAAH
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCHHH
LIARYSGKPVINAGDGRRAHPTQALLDAYTIRQEYGSLAGKKVAIIGDIRHSRVARSNAE
HHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHCCHH
LLPKLGAEVVLAGPATLLPADLAALPGVTVTTDPKEAVHGAHAVMALRLQQERMNAGYLA
HHHHCCCCEEEECCCHHCCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHH
SLQEYVERYQVNEALLREAESGAIVLHPGPLNRDLEISSEVADGPQSRILKQVENGQAVR
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCHHH
MSVLYHLLVGRD
HHHHHHHHHCCC
>Mature Secondary Structure 
TVLAPPRPRSLLDFQDWLPERLSALLDNADTMHQVLERPVRKVPALQGLTVCTAFFENS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
TRTRISFELAARRMSADVISFAAGASSLSKGESLRDTVEVLSAYKVDAFVVRHPASGAAH
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHEEEEECCCCCHHH
LIARYSGKPVINAGDGRRAHPTQALLDAYTIRQEYGSLAGKKVAIIGDIRHSRVARSNAE
HHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHCCHH
LLPKLGAEVVLAGPATLLPADLAALPGVTVTTDPKEAVHGAHAVMALRLQQERMNAGYLA
HHHHCCCCEEEECCCHHCCHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHH
SLQEYVERYQVNEALLREAESGAIVLHPGPLNRDLEISSEVADGPQSRILKQVENGQAVR
HHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCHHH
MSVLYHLLVGRD
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA