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The map label for this gene is ruvB [H]

Identifier: 94984512

GI number: 94984512

Start: 410942

End: 411937

Strand: Reverse

Name: ruvB [H]

Synonym: Dgeo_0404

Alternate gene names: 94984512

Gene position: 411937-410942 (Counterclockwise)

Preceding gene: 94984513

Following gene: 94984511

Centisome position: 16.7

GC content: 64.76

Gene sequence:

>996_bases
ATGACCGAACCGCTCGACGCCGCCCTGCGCCCCAAGAGCCTTGCGGAGTATGTGGGTCAGGCGCGGCTCAAGGAAAAACT
GGGGGTGTATCTCCAGGCTGCCAAGGGCCGCAAAGAGGCCTTGGATCACACCTTGCTCTTCGGCCCGCCCGGCCTGGGCA
AAACCACGCTGGCACACATCATCGCCGCCGAACTGGGCGTGAATATTCGGGTTACCTCCGGCCCGGCAATCGAGAAGCCA
GGGGACCTCGCCGCCATCCTCACCAACAGCCTGGAGGAAGGCGACGTGCTGTTCATCGATGAGATTCACCGACTCGGACG
GGTGGCAGAAGAACACCTCTACCCCGCGATGGAGGATTTCAAGCTGGACATTGTGCTGGGGCAAGGTCCAGCGGCCCGCA
CCATCGAGCTGCCGCTGCCGCGCTTTACGCTGGTGGGCGCCACCACTCGCCCCGGCCTGATCACCGCGCCGATGCGCTCG
CGCTTTGGGATCATCGAGCATCTGGAGTACTACACGCCGGAGGAGATCGGAACCAATCTGCTGCGTGACGCCCGTCTGCT
GGGATTTGGCCTGACCGAGGAGGCGGCCATCGAGATCGGCGCCCGTTCACGGGGCACCATGCGTATTGCCAAACGCCTGC
TGCGGCGCGTGCGCGACTATGCGGACGTGGCGGGCGAGAAGACCATCGAGCTGGAACGCGCGCAGGACGCCCTGGACAAA
CTGGGGCTAGACACGGCCGGACTAGACGAGCGCGACAAGAAGTACCTCGAAACCCTGATCCACCGCTTTGCAGGCGGTCC
GGTCGGCGTAGACACCCTCGCCACCGCGATCTCGGAGGACGCCCTGACGCTGGAAGACGTGTATGAGCCGTACCTGATTC
AGCTCGGTTTCATCAAGCGCACCCCGCGCGGGCGCGTTGCCACCGCGCATGCCTACGACCACCTGGGGCTGCCGGTGGGG
GCGGCAGAAAGCGACCTGGGCCTGTATACGAATTGA

Upstream 100 bases:

>100_bases
AGAACCGAGAAGAGGGGCAATGCGGTCTTGCCGCCGAGAGACAAGAGCCCTCTTGGCCTTACCCAGACTCTTGATTTCGG
AAATAGCGTAATATAAGAGT

Downstream 100 bases:

>100_bases
GGCGCCGAGGCTCTAGCTTCTTCCTCGCGCCTCCAACCTGACCCCTCACCCCTCGCTACACTTCCCTTATGCCCGCGCCG
CAAGTCCAGATGTTTGGCCT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTEPLDAALRPKSLAEYVGQARLKEKLGVYLQAAKGRKEALDHTLLFGPPGLGKTTLAHIIAAELGVNIRVTSGPAIEKP
GDLAAILTNSLEEGDVLFIDEIHRLGRVAEEHLYPAMEDFKLDIVLGQGPAARTIELPLPRFTLVGATTRPGLITAPMRS
RFGIIEHLEYYTPEEIGTNLLRDARLLGFGLTEEAAIEIGARSRGTMRIAKRLLRRVRDYADVAGEKTIELERAQDALDK
LGLDTAGLDERDKKYLETLIHRFAGGPVGVDTLATAISEDALTLEDVYEPYLIQLGFIKRTPRGRVATAHAYDHLGLPVG
AAESDLGLYTN

Sequences:

>Translated_331_residues
MTEPLDAALRPKSLAEYVGQARLKEKLGVYLQAAKGRKEALDHTLLFGPPGLGKTTLAHIIAAELGVNIRVTSGPAIEKP
GDLAAILTNSLEEGDVLFIDEIHRLGRVAEEHLYPAMEDFKLDIVLGQGPAARTIELPLPRFTLVGATTRPGLITAPMRS
RFGIIEHLEYYTPEEIGTNLLRDARLLGFGLTEEAAIEIGARSRGTMRIAKRLLRRVRDYADVAGEKTIELERAQDALDK
LGLDTAGLDERDKKYLETLIHRFAGGPVGVDTLATAISEDALTLEDVYEPYLIQLGFIKRTPRGRVATAHAYDHLGLPVG
AAESDLGLYTN
>Mature_330_residues
TEPLDAALRPKSLAEYVGQARLKEKLGVYLQAAKGRKEALDHTLLFGPPGLGKTTLAHIIAAELGVNIRVTSGPAIEKPG
DLAAILTNSLEEGDVLFIDEIHRLGRVAEEHLYPAMEDFKLDIVLGQGPAARTIELPLPRFTLVGATTRPGLITAPMRSR
FGIIEHLEYYTPEEIGTNLLRDARLLGFGLTEEAAIEIGARSRGTMRIAKRLLRRVRDYADVAGEKTIELERAQDALDKL
GLDTAGLDERDKKYLETLIHRFAGGPVGVDTLATAISEDALTLEDVYEPYLIQLGFIKRTPRGRVATAHAYDHLGLPVGA
AESDLGLYTN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=312, Percent_Identity=57.3717948717949, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1787119, Length=255, Percent_Identity=29.0196078431373, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36120; Mature: 35989

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLDAALRPKSLAEYVGQARLKEKLGVYLQAAKGRKEALDHTLLFGPPGLGKTTLAHI
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEECCCCCCHHHHHHH
IAAELGVNIRVTSGPAIEKPGDLAAILTNSLEEGDVLFIDEIHRLGRVAEEHLYPAMEDF
HHHHHCCEEEECCCCCCCCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHCC
KLDIVLGQGPAARTIELPLPRFTLVGATTRPGLITAPMRSRFGIIEHLEYYTPEEIGTNL
EEEEEECCCCCCCEEECCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHH
LRDARLLGFGLTEEAAIEIGARSRGTMRIAKRLLRRVRDYADVAGEKTIELERAQDALDK
HHHHHHHCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
LGLDTAGLDERDKKYLETLIHRFAGGPVGVDTLATAISEDALTLEDVYEPYLIQLGFIKR
HCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEHHHHCCCHHEEEHHEEE
TPRGRVATAHAYDHLGLPVGAAESDLGLYTN
CCCCCEEEEEHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEPLDAALRPKSLAEYVGQARLKEKLGVYLQAAKGRKEALDHTLLFGPPGLGKTTLAHI
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEECCCCCCHHHHHHH
IAAELGVNIRVTSGPAIEKPGDLAAILTNSLEEGDVLFIDEIHRLGRVAEEHLYPAMEDF
HHHHHCCEEEECCCCCCCCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHCC
KLDIVLGQGPAARTIELPLPRFTLVGATTRPGLITAPMRSRFGIIEHLEYYTPEEIGTNL
EEEEEECCCCCCCEEECCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHCCHHHHHHHH
LRDARLLGFGLTEEAAIEIGARSRGTMRIAKRLLRRVRDYADVAGEKTIELERAQDALDK
HHHHHHHCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
LGLDTAGLDERDKKYLETLIHRFAGGPVGVDTLATAISEDALTLEDVYEPYLIQLGFIKR
HCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEHHHHCCCHHEEEHHEEE
TPRGRVATAHAYDHLGLPVGAAESDLGLYTN
CCCCCEEEEEHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9447236; 10567266 [H]