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The map label for this gene is pnp

Identifier: 94984509

GI number: 94984509

Start: 406071

End: 408236

Strand: Reverse

Name: pnp

Synonym: Dgeo_0401

Alternate gene names: 94984509

Gene position: 408236-406071 (Counterclockwise)

Preceding gene: 94984511

Following gene: 94984508

Centisome position: 16.55

GC content: 67.36

Gene sequence:

>2166_bases
ATGATTGCAAAGACCTACACCACGATGCTCGGCGGGCGCGAACTGAGCATCGAGACGGGCAAACTGGCAAAGCTCGCCAG
CGGCAGCGTCACCCTGCGCTACGGCGATACGCTGTTGCTCGTCACCGCCCAGGCACGCGAGGAACGCAGCACCCTGGACT
TTCTGCCCCTGACGGTCGAGTTCGAGGAACGGCATTACGCGGTCGGGAGGATTCCCGGCTCCTTCCACCGCCGCGAGGGT
CGGCCCGGCGAACACGCCATTCTCAGCGCGCGCATCACCGACCGCCAGATTCGGCCCCTTTTCCCCAAAGGGTACCGCCA
AGAGACCCAGGTCATCATCACGGTGCTTTCGGCCGACCAGCAAAACGCGCCGGACGTGCTCGGGGCCATCGGCGCCTCGG
CGGCCCTCAGCCTCAGCGACATTCCCTGGAACGGGCCGACCGCCTGCGTGCGCGTCGGGATGATCGATGGCGAGTATGTG
ATCAACCCCACCAGCGATCAGCTCACGCGCTCCAGCCTCGACCTGGTGGTGGCGGGCACCCGTGACGCCGTCAACATGGT
GGAAGCGGGCGCGCAGGGCGTCAGCGAGGAGACGTTGGTGGGCGCCATCGAGTTCGCGCACCGCGAGCTGCAGGGCGTGC
TGGATCTGATCGAGACGATGCGCGCCGAGGTGGGCCGCGAGAAGTTCAACTTTCTGGTCGACAGTGACCTCAGCACCGAC
CTGGTGCCCGAGCTGAGCGAGGCGGCGCGGGCGGCGGGGCTGCGGGACGCGCTGCTGACCACCAAAAAGCAGGAGCGCAG
CGCAAACCTCAAGGCGCTGCGGGACCGCTTGATCGCGGAGCGCGTGCCGGATCCGGAAGCTGCGGGAGCGGCGGAGCAGA
TTGAAGCGCTCAAGGCGGCCTTCGCCAAGGTGGAGAAACAGGAGCTGCGCCGCCTGATTCTGCAAGAAGACCTGCGGGCG
GATGGGCGCAACTCGAAGACGGTGCGTCCGATCTGGATTGAAGCGCGGCCCCTGCCCCGCGCACACGGCAGCGCGATCTT
CACGCGCGGAGAGACGCAAGTGCTGGGCGTCACCACCCTGGGCACCGAGCGCGACGAGCTGCTGGTGGACAATCTGACCG
GCGAGACGAATGACCGCTTCCTGCTGCACTACAACTTCCCGCCCTACTCGACCGGCGAGGTCAAGCGGGTGGGCGGGCAG
TCGCGCCGCGAGGTGGGGCACGGCAACCTCGCCAAGCGGGCCATCCGCGCGGTGCTGCCCGCCTTCGACGACTTCCCCTA
CGTGATCCGCGTGGTGGGCGAGGTGCTGGAGTCCAACGGCTCGAGCAGCATGGCGACCGTCTGCGCCGGCACCCTCAGTC
TGATGGACGCGGGCGTGCCCATTCAGGCACCGGTGGCAGGTGTGGCGATGGGCCTGGTGATGGAGGGCGAGCAATACCGC
ATCCTGACCGACATCCTGGGCCTGGAAGACGCGCTGGGCGACATGGACTTTAAGGTCTGTGGCACGGCGGAGGGCGTCAC
GGCGTTGCAGATGGACATCAAGGTGAGCGGCGTAACTCCGGCGATCATGCGAGAAGCGCTCGCGCAGGCCCGCGAGGCGC
GGCTGCACATCCTAGGCAAGATGGCCGAGGTGCTGCCCGCTCCGCGTCCCGAACTCTCCCCCACCGCCCCGCGCATCCTG
ACCCTCAAGATCAACCCCGAGCTGATCGGCAAGGTGATCGGCCCCGGCGGCAAGCAGGTGCGCGAGCTGGAGGCCATGGG
CGCGCAGGTGACCATCGAGGAGGACGGCAACATCCGGATTTTCAGTGCCGATGGAGCGGCCGCTGAGGCCGTGCGCCAGA
AGATCGAGGGCCTGACCCGCGAGGCCAAGGTCGGCGAGGAGTACGAGGGCACCGTGGTCAAGACGGCCCCCTTCGGCGCC
TTTGTTAACCTGTTTCCTGGCCAGGACGGAATGCTGCACATCAGCCAGATGAGCGAGAACCGCGTCAACGCCGTTGAGGA
CGTGCTGAACGTGGGCGATCGGCTGCGGGTCAAGATCGCCAATATCGATGACCGAGGCAAAATCGATCTGATTCGCCCGG
AGCTGGAGGGCAAGATTGCCCCCCGTGAACCACGCGCCGCCCGCGCAGGGGGCGACCGGGGCGGACGTCCGCCGCGGCGG
GAGTAG

Upstream 100 bases:

>100_bases
CACTCCCCACGCCCAGCACCAGGTCACACGGGACGCGCCCCGGCCACCAACCTCCACCGGGCATCCATTTGTCTGCTTGG
ACGCACGTTAGAATTGTTTG

Downstream 100 bases:

>100_bases
GCCACCGTTGAGGAGAGTCACTTCCGTGTGGGGCCGCCCTCCGGCTATTCGTTGGCGGTGAATACTGCCAGGGCGGCGAC
CACCCATGCCCCCACAGCAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MIAKTYTTMLGGRELSIETGKLAKLASGSVTLRYGDTLLLVTAQAREERSTLDFLPLTVEFEERHYAVGRIPGSFHRREG
RPGEHAILSARITDRQIRPLFPKGYRQETQVIITVLSADQQNAPDVLGAIGASAALSLSDIPWNGPTACVRVGMIDGEYV
INPTSDQLTRSSLDLVVAGTRDAVNMVEAGAQGVSEETLVGAIEFAHRELQGVLDLIETMRAEVGREKFNFLVDSDLSTD
LVPELSEAARAAGLRDALLTTKKQERSANLKALRDRLIAERVPDPEAAGAAEQIEALKAAFAKVEKQELRRLILQEDLRA
DGRNSKTVRPIWIEARPLPRAHGSAIFTRGETQVLGVTTLGTERDELLVDNLTGETNDRFLLHYNFPPYSTGEVKRVGGQ
SRREVGHGNLAKRAIRAVLPAFDDFPYVIRVVGEVLESNGSSSMATVCAGTLSLMDAGVPIQAPVAGVAMGLVMEGEQYR
ILTDILGLEDALGDMDFKVCGTAEGVTALQMDIKVSGVTPAIMREALAQAREARLHILGKMAEVLPAPRPELSPTAPRIL
TLKINPELIGKVIGPGGKQVRELEAMGAQVTIEEDGNIRIFSADGAAAEAVRQKIEGLTREAKVGEEYEGTVVKTAPFGA
FVNLFPGQDGMLHISQMSENRVNAVEDVLNVGDRLRVKIANIDDRGKIDLIRPELEGKIAPREPRAARAGGDRGGRPPRR
E

Sequences:

>Translated_721_residues
MIAKTYTTMLGGRELSIETGKLAKLASGSVTLRYGDTLLLVTAQAREERSTLDFLPLTVEFEERHYAVGRIPGSFHRREG
RPGEHAILSARITDRQIRPLFPKGYRQETQVIITVLSADQQNAPDVLGAIGASAALSLSDIPWNGPTACVRVGMIDGEYV
INPTSDQLTRSSLDLVVAGTRDAVNMVEAGAQGVSEETLVGAIEFAHRELQGVLDLIETMRAEVGREKFNFLVDSDLSTD
LVPELSEAARAAGLRDALLTTKKQERSANLKALRDRLIAERVPDPEAAGAAEQIEALKAAFAKVEKQELRRLILQEDLRA
DGRNSKTVRPIWIEARPLPRAHGSAIFTRGETQVLGVTTLGTERDELLVDNLTGETNDRFLLHYNFPPYSTGEVKRVGGQ
SRREVGHGNLAKRAIRAVLPAFDDFPYVIRVVGEVLESNGSSSMATVCAGTLSLMDAGVPIQAPVAGVAMGLVMEGEQYR
ILTDILGLEDALGDMDFKVCGTAEGVTALQMDIKVSGVTPAIMREALAQAREARLHILGKMAEVLPAPRPELSPTAPRIL
TLKINPELIGKVIGPGGKQVRELEAMGAQVTIEEDGNIRIFSADGAAAEAVRQKIEGLTREAKVGEEYEGTVVKTAPFGA
FVNLFPGQDGMLHISQMSENRVNAVEDVLNVGDRLRVKIANIDDRGKIDLIRPELEGKIAPREPRAARAGGDRGGRPPRR
E
>Mature_721_residues
MIAKTYTTMLGGRELSIETGKLAKLASGSVTLRYGDTLLLVTAQAREERSTLDFLPLTVEFEERHYAVGRIPGSFHRREG
RPGEHAILSARITDRQIRPLFPKGYRQETQVIITVLSADQQNAPDVLGAIGASAALSLSDIPWNGPTACVRVGMIDGEYV
INPTSDQLTRSSLDLVVAGTRDAVNMVEAGAQGVSEETLVGAIEFAHRELQGVLDLIETMRAEVGREKFNFLVDSDLSTD
LVPELSEAARAAGLRDALLTTKKQERSANLKALRDRLIAERVPDPEAAGAAEQIEALKAAFAKVEKQELRRLILQEDLRA
DGRNSKTVRPIWIEARPLPRAHGSAIFTRGETQVLGVTTLGTERDELLVDNLTGETNDRFLLHYNFPPYSTGEVKRVGGQ
SRREVGHGNLAKRAIRAVLPAFDDFPYVIRVVGEVLESNGSSSMATVCAGTLSLMDAGVPIQAPVAGVAMGLVMEGEQYR
ILTDILGLEDALGDMDFKVCGTAEGVTALQMDIKVSGVTPAIMREALAQAREARLHILGKMAEVLPAPRPELSPTAPRIL
TLKINPELIGKVIGPGGKQVRELEAMGAQVTIEEDGNIRIFSADGAAAEAVRQKIEGLTREAKVGEEYEGTVVKTAPFGA
FVNLFPGQDGMLHISQMSENRVNAVEDVLNVGDRLRVKIANIDDRGKIDLIRPELEGKIAPREPRAARAGGDRGGRPPRR
E

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=698, Percent_Identity=38.3954154727794, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI145693187, Length=685, Percent_Identity=49.1970802919708, Blast_Score=627, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=740, Percent_Identity=33.6486486486486, Blast_Score=344, Evalue=9e-95,
Organism=Drosophila melanogaster, GI281362905, Length=720, Percent_Identity=38.75, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651641, Length=720, Percent_Identity=38.75, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24651643, Length=720, Percent_Identity=38.75, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI161079377, Length=664, Percent_Identity=39.1566265060241, Blast_Score=424, Evalue=1e-118,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_DEIGD (Q1J1D0)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603873.1
- ProteinModelPortal:   Q1J1D0
- SMR:   Q1J1D0
- STRING:   Q1J1D0
- GeneID:   4057137
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0401
- NMPDR:   fig|68909.1.peg.312
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q1J1D0
- ProtClustDB:   PRK11824
- BioCyc:   DGEO319795:DGEO_0401-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78097; Mature: 78097

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAKTYTTMLGGRELSIETGKLAKLASGSVTLRYGDTLLLVTAQAREERSTLDFLPLTVE
CCCCHHHHHHCCEEEEEECCCHHEECCCCEEEEECCEEEEEEECCCCCCCCEEEEEEEEE
FEERHYAVGRIPGSFHRREGRPGEHAILSARITDRQIRPLFPKGYRQETQVIITVLSADQ
ECCCEEEEECCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCC
QNAPDVLGAIGASAALSLSDIPWNGPTACVRVGMIDGEYVINPTSDQLTRSSLDLVVAGT
CCCCHHHHHHCCHHEEEECCCCCCCCCCEEEEEEECCCEEECCCHHHHHHCCCCEEEECC
RDAVNMVEAGAQGVSEETLVGAIEFAHRELQGVLDLIETMRAEVGREKFNFLVDSDLSTD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEECCCCCCC
LVPELSEAARAAGLRDALLTTKKQERSANLKALRDRLIAERVPDPEAAGAAEQIEALKAA
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FAKVEKQELRRLILQEDLRADGRNSKTVRPIWIEARPLPRAHGSAIFTRGETQVLGVTTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEEEEEEEC
GTERDELLVDNLTGETNDRFLLHYNFPPYSTGEVKRVGGQSRREVGHGNLAKRAIRAVLP
CCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHCCCCHHHHCCCCHHHHHHHHHHHC
AFDDFPYVIRVVGEVLESNGSSSMATVCAGTLSLMDAGVPIQAPVAGVAMGLVMEGEQYR
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEE
ILTDILGLEDALGDMDFKVCGTAEGVTALQMDIKVSGVTPAIMREALAQAREARLHILGK
HHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MAEVLPAPRPELSPTAPRILTLKINPELIGKVIGPGGKQVRELEAMGAQVTIEEDGNIRI
HHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCEEE
FSADGAAAEAVRQKIEGLTREAKVGEEYEGTVVKTAPFGAFVNLFPGQDGMLHISQMSEN
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCEEEEEHHCCC
RVNAVEDVLNVGDRLRVKIANIDDRGKIDLIRPELEGKIAPREPRAARAGGDRGGRPPRR
HHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCC
E
C
>Mature Secondary Structure
MIAKTYTTMLGGRELSIETGKLAKLASGSVTLRYGDTLLLVTAQAREERSTLDFLPLTVE
CCCCHHHHHHCCEEEEEECCCHHEECCCCEEEEECCEEEEEEECCCCCCCCEEEEEEEEE
FEERHYAVGRIPGSFHRREGRPGEHAILSARITDRQIRPLFPKGYRQETQVIITVLSADQ
ECCCEEEEECCCCHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCC
QNAPDVLGAIGASAALSLSDIPWNGPTACVRVGMIDGEYVINPTSDQLTRSSLDLVVAGT
CCCCHHHHHHCCHHEEEECCCCCCCCCCEEEEEEECCCEEECCCHHHHHHCCCCEEEECC
RDAVNMVEAGAQGVSEETLVGAIEFAHRELQGVLDLIETMRAEVGREKFNFLVDSDLSTD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCEEECCCCCCC
LVPELSEAARAAGLRDALLTTKKQERSANLKALRDRLIAERVPDPEAAGAAEQIEALKAA
HHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
FAKVEKQELRRLILQEDLRADGRNSKTVRPIWIEARPLPRAHGSAIFTRGETQVLGVTTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEEEEEEEC
GTERDELLVDNLTGETNDRFLLHYNFPPYSTGEVKRVGGQSRREVGHGNLAKRAIRAVLP
CCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHCCCCHHHHCCCCHHHHHHHHHHHC
AFDDFPYVIRVVGEVLESNGSSSMATVCAGTLSLMDAGVPIQAPVAGVAMGLVMEGEQYR
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEE
ILTDILGLEDALGDMDFKVCGTAEGVTALQMDIKVSGVTPAIMREALAQAREARLHILGK
HHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
MAEVLPAPRPELSPTAPRILTLKINPELIGKVIGPGGKQVRELEAMGAQVTIEEDGNIRI
HHHHCCCCCCCCCCCCCEEEEEEECHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCEEE
FSADGAAAEAVRQKIEGLTREAKVGEEYEGTVVKTAPFGAFVNLFPGQDGMLHISQMSEN
EECCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCEEEEEHHCCC
RVNAVEDVLNVGDRLRVKIANIDDRGKIDLIRPELEGKIAPREPRAARAGGDRGGRPPRR
HHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA