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The map label for this gene is metK

Identifier: 94984395

GI number: 94984395

Start: 281378

End: 282604

Strand: Reverse

Name: metK

Synonym: Dgeo_0287

Alternate gene names: 94984395

Gene position: 282604-281378 (Counterclockwise)

Preceding gene: 94984396

Following gene: 94984391

Centisome position: 11.45

GC content: 66.01

Gene sequence:

>1227_bases
ATGCGGAAGTTTTACACGTCGGAGTCGGTGTCGGAAGGCCACCCGGACAAGCTGGCGGATTTTATCTCGGACTCGCTGCT
GGACGAGTTCCTGCGTCAGGAACCTGAAAGCCGCGTGGCGGTGGAAACGCTGGTCACGACCGGCATGGCGGTTGTGGCCG
GCGAGGTGCGCGCCCGGAGCGCCCACGTGGATGTGCAGAAAGTGGTGCGTGAAGCCGTGCAGAAGGTGGGCTACACCCGC
GCCAACTACGGCTTTGACGCCGAATACAGCGCGGTACTGGTGTCGATCCACGAGCAGAGCCCCGAAATCGGCGGCACGGT
GGACCGTTCCGAGGAGTGGCGCCAGATGACGCCCCAAGAACGCGCGCGTCCCGAGAACACCTTCTCGGAGGTCGGAGCGG
GCGATCAGGGCCTGATGTTCGGCTACGCCACCGACGAGACGCCCGAACTGATGCCGCTCCCCATCTCGCTGGCGCACCGA
CTCACCCGGCGCCTGGCCGAGCTGCGTAAGAGTGGCGCGCTCCCCTACCTGCGCCCCGATGCCAAGGCCCAGGTGACGGT
CGTGCGCAACGGCGAGCCGCATGAGGCCACCGAAACCCACGTGGACACCATCGTGATCAGCACCCAGCACAGCGAGGACG
TGAGCCAAGAGCAAATCGAGGCAGACATGCTCGAACACGTGGTCCGCGCCGTGATCCCCCACGAGCTGCTCACGCCTGAA
ACCAAATTCTTTATCAATCCCAGCGGGCGCTTCGTGATCGGCGGCCCACACGGTGACACCGGTCTGACCGGGCGCAAAAT
CATCGTGGACACCTACGGCGGGGCCGTGCCGCATGGCGGCGGCGCCTTTTCCGGCAAGGACCCCACGAAGGTGGACCGTT
CAGCGGCCTACTACGCGCGCTATATCGCCAAGAACATCGTGGCGGCGGGGCTGGCGCGGCGGGCGCTGGTGGAAGTGGCC
TACGCGATCGGACGCGCGCATCCGGTCAGCCTGCGGGTGGACACCTACGGCACAGGCACGCTGAGTGACGAGCGGCTGGC
CGACCTGGTCCGCGCGCACTTTGACGCCCGGCCTCAGGCCATCATTGCAGAACTGGACCTCCAGCGCCCAATCTACGCGC
AGACTGCGGCCTATGGGCACTTCGGCCGCCCCGAGTTCCCCTGGGAACAGACGAACAAGGCGGAGGCGCTGCGCAGAGCC
GCCGAGGAATTGAAGCAAGAAGCGTAA

Upstream 100 bases:

>100_bases
CGGTACCGCCCTCACGTGAAGGCGGGCCGTGCGGGGACCGCAGCGACATCCCTCTCCAGGTGGTTCTGTGAGACGCCCCC
CCGCGCGCAGAGGAGCGAAG

Downstream 100 bases:

>100_bases
GCACAGGGCCGGGGAGACAGTGCCCCGGCCCGCGGCTTTCATGTCTTGCTCTCTGCGGCCTGCTGTGCCCCGCGCTCTCC
TGCCGAGCTTAGTTCTGCCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MRKFYTSESVSEGHPDKLADFISDSLLDEFLRQEPESRVAVETLVTTGMAVVAGEVRARSAHVDVQKVVREAVQKVGYTR
ANYGFDAEYSAVLVSIHEQSPEIGGTVDRSEEWRQMTPQERARPENTFSEVGAGDQGLMFGYATDETPELMPLPISLAHR
LTRRLAELRKSGALPYLRPDAKAQVTVVRNGEPHEATETHVDTIVISTQHSEDVSQEQIEADMLEHVVRAVIPHELLTPE
TKFFINPSGRFVIGGPHGDTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSAAYYARYIAKNIVAAGLARRALVEVA
YAIGRAHPVSLRVDTYGTGTLSDERLADLVRAHFDARPQAIIAELDLQRPIYAQTAAYGHFGRPEFPWEQTNKAEALRRA
AEELKQEA

Sequences:

>Translated_408_residues
MRKFYTSESVSEGHPDKLADFISDSLLDEFLRQEPESRVAVETLVTTGMAVVAGEVRARSAHVDVQKVVREAVQKVGYTR
ANYGFDAEYSAVLVSIHEQSPEIGGTVDRSEEWRQMTPQERARPENTFSEVGAGDQGLMFGYATDETPELMPLPISLAHR
LTRRLAELRKSGALPYLRPDAKAQVTVVRNGEPHEATETHVDTIVISTQHSEDVSQEQIEADMLEHVVRAVIPHELLTPE
TKFFINPSGRFVIGGPHGDTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSAAYYARYIAKNIVAAGLARRALVEVA
YAIGRAHPVSLRVDTYGTGTLSDERLADLVRAHFDARPQAIIAELDLQRPIYAQTAAYGHFGRPEFPWEQTNKAEALRRA
AEELKQEA
>Mature_408_residues
MRKFYTSESVSEGHPDKLADFISDSLLDEFLRQEPESRVAVETLVTTGMAVVAGEVRARSAHVDVQKVVREAVQKVGYTR
ANYGFDAEYSAVLVSIHEQSPEIGGTVDRSEEWRQMTPQERARPENTFSEVGAGDQGLMFGYATDETPELMPLPISLAHR
LTRRLAELRKSGALPYLRPDAKAQVTVVRNGEPHEATETHVDTIVISTQHSEDVSQEQIEADMLEHVVRAVIPHELLTPE
TKFFINPSGRFVIGGPHGDTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSAAYYARYIAKNIVAAGLARRALVEVA
YAIGRAHPVSLRVDTYGTGTLSDERLADLVRAHFDARPQAIIAELDLQRPIYAQTAAYGHFGRPEFPWEQTNKAEALRRA
AEELKQEA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=392, Percent_Identity=54.3367346938776, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI4557737, Length=392, Percent_Identity=53.5714285714286, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1789311, Length=400, Percent_Identity=56.25, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=52.9562982005141, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=52.1850899742931, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25145633, Length=390, Percent_Identity=51.5384615384615, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551082, Length=387, Percent_Identity=51.421188630491, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=52.3255813953488, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=48.0719794344473, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=50.7246376811594, Blast_Score=337, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI32565903, Length=316, Percent_Identity=52.5316455696203, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI32565909, Length=114, Percent_Identity=38.5964912280702, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323209, Length=387, Percent_Identity=51.421188630491, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320710, Length=392, Percent_Identity=50.765306122449, Blast_Score=395, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45552159, Length=394, Percent_Identity=52.0304568527919, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580529, Length=394, Percent_Identity=52.0304568527919, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580537, Length=394, Percent_Identity=52.0304568527919, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580533, Length=394, Percent_Identity=52.0304568527919, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580535, Length=394, Percent_Identity=52.0304568527919, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580531, Length=394, Percent_Identity=52.0304568527919, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=51.5228426395939, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=51.5228426395939, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580541, Length=382, Percent_Identity=50.7853403141361, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24580539, Length=135, Percent_Identity=45.9259259259259, Blast_Score=116, Evalue=4e-26,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_DEIGD (Q1J1P4)

Other databases:

- EMBL:   CP000359
- RefSeq:   YP_603759.1
- ProteinModelPortal:   Q1J1P4
- SMR:   Q1J1P4
- STRING:   Q1J1P4
- GeneID:   4058011
- GenomeReviews:   CP000359_GR
- KEGG:   dge:Dgeo_0287
- NMPDR:   fig|68909.1.peg.2313
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q1J1P4
- ProtClustDB:   PRK05250
- BioCyc:   DGEO319795:DGEO_0287-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44939; Mature: 44939

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFYTSESVSEGHPDKLADFISDSLLDEFLRQEPESRVAVETLVTTGMAVVAGEVRARS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHH
AHVDVQKVVREAVQKVGYTRANYGFDAEYSAVLVSIHEQSPEIGGTVDRSEEWRQMTPQE
HCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCHH
RARPENTFSEVGAGDQGLMFGYATDETPELMPLPISLAHRLTRRLAELRKSGALPYLRPD
HCCCCHHHHHHCCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCC
AKAQVTVVRNGEPHEATETHVDTIVISTQHSEDVSQEQIEADMLEHVVRAVIPHELLTPE
CCEEEEEEECCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TKFFINPSGRFVIGGPHGDTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSAAYYAR
CEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YIAKNIVAAGLARRALVEVAYAIGRAHPVSLRVDTYGTGTLSDERLADLVRAHFDARPQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHH
IIAELDLQRPIYAQTAAYGHFGRPEFPWEQTNKAEALRRAAEELKQEA
HHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRKFYTSESVSEGHPDKLADFISDSLLDEFLRQEPESRVAVETLVTTGMAVVAGEVRARS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHH
AHVDVQKVVREAVQKVGYTRANYGFDAEYSAVLVSIHEQSPEIGGTVDRSEEWRQMTPQE
HCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHCCCHH
RARPENTFSEVGAGDQGLMFGYATDETPELMPLPISLAHRLTRRLAELRKSGALPYLRPD
HCCCCHHHHHHCCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCC
AKAQVTVVRNGEPHEATETHVDTIVISTQHSEDVSQEQIEADMLEHVVRAVIPHELLTPE
CCEEEEEEECCCCCCCHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TKFFINPSGRFVIGGPHGDTGLTGRKIIVDTYGGAVPHGGGAFSGKDPTKVDRSAAYYAR
CEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YIAKNIVAAGLARRALVEVAYAIGRAHPVSLRVDTYGTGTLSDERLADLVRAHFDARPQA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHH
IIAELDLQRPIYAQTAAYGHFGRPEFPWEQTNKAEALRRAAEELKQEA
HHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA