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The map label for this gene is prfB [H]

Identifier: 94984277

GI number: 94984277

Start: 156077

End: 157219

Strand: Reverse

Name: prfB [H]

Synonym: Dgeo_0169

Alternate gene names: 94984277

Gene position: 157219-156077 (Counterclockwise)

Preceding gene: 94984278

Following gene: 94984276

Centisome position: 6.37

GC content: 62.55

Gene sequence:

>1143_bases
ATGCCCGCGCCCCGCTCAACGGGAGAAACGCGGAAGGGAGACACGTCATGCAGGAACTGCTGGAAAAACTGGCGTCGCTC
CGGGAGTACCTTTGACATTCCCGGCAAGACGCGAAGGTTGAACGAACTCGACCGCGAACTCAGTGACCCGGAACTGTGGA
ACAACCCCAGCCGTGCTCGGCAAGTCACACAGGAGGCCGGGAGCCTGCGCCGCATCGTGGACGGCTACAGGACGCTGCAG
TCAGATGCAAACAGTCTCAACGAGATGCTGGAGATGGCGAGCGACGAGGAACGCGCGCTGCTGGCCGAGGAGCAGGACAC
TATCCAGGCGCGGGTGGATGAGCTGTACCGCGAGACCCTCTTCACCATGAAACACGCTGACGCCCCCGCTATCGTGCGGG
TTAAGGGGGGCGCGGGCGGCACGGAGGCGCAAGACTGGGCCGGGATGCTCGCGCGCATGTACATGCGTTGGGCCGAGCGG
CGAGGCTATAAGGTGGACGTGCTCGACGAGCAGCCCGGTGAGCAGGCCGGCTACCAGAGCATCGAATTCATCATCCGTGG
GGAAAAGGCATTCGGGATGATGGCGCCTGAACACGGCGTCCATCGCCTGGTGCGCGTCTCGCCCTTCGATGCCAACAACC
GCCGCCAGACCTCCTTCGCGTCCGTGGACGTAGTGCCTGAGGTGCCGGAAGAGCAGATCGACATCGTGATTCCCGATTCT
GACGTACGGGTGGACGTGTACCGCTCACAGGGTGCCGGCGGGCAAGGGGTGAACACGACGGACTCGGCAGTGCGCGTCAC
CCACCTCCCCACTGGGATCATCGTGGCCATTCAGGTCACACGCTCGCAGATCAAGAACCGCGAGATGGCCTTCCAGATCC
TCAAACAGCGCCTCTACGACTTGGAGATGCGCAAGCGCGAAGAGGAAGAAGCCAAGGCACGCGGCGAACAAAAGAAGATC
GAGTGGGGCTCGCAGATCCGCTCCTACGTGCTCGATAAGCAGTATGTCAAGGACCACCGCACCGGACTCATGAAGCACAA
CCCCGATGACGTGCTCGACGGTGATCTCGACGACCTGATGTGGGCGGGCCTAGAATGGATGGCTGGGAAGCGGGCCGCCG
AGGACGGTGCTGACGAAGAATAA

Upstream 100 bases:

>100_bases
TCCACTGCCTTTTTGTGCAGCGCTCCCAGCGATTCCCAGCCGGTTTCTTGCCCCAAGGCTGCCGGAATGCAGCGCCTCAC
CCTATACTCCCCTACAGCAG

Downstream 100 bases:

>100_bases
GCCGAATCAGCCCAAACGTTGCCGGAGGCGCCAACACTGGCCCTCCGCTTTTTTTTGTTTTTCTTACTCGCCGCCAGGAT
TGATGAGAAATGGGTGAAGG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MPAPRSTGETRKGDTSCRNCWKNWRRSGSTFDIPGKTRRLNELDRELSDPELWNNPSRARQVTQEAGSLRRIVDGYRTLQ
SDANSLNEMLEMASDEERALLAEEQDTIQARVDELYRETLFTMKHADAPAIVRVKGGAGGTEAQDWAGMLARMYMRWAER
RGYKVDVLDEQPGEQAGYQSIEFIIRGEKAFGMMAPEHGVHRLVRVSPFDANNRRQTSFASVDVVPEVPEEQIDIVIPDS
DVRVDVYRSQGAGGQGVNTTDSAVRVTHLPTGIIVAIQVTRSQIKNREMAFQILKQRLYDLEMRKREEEEAKARGEQKKI
EWGSQIRSYVLDKQYVKDHRTGLMKHNPDDVLDGDLDDLMWAGLEWMAGKRAAEDGADEE

Sequences:

>Translated_380_residues
MPAPRSTGETRKGDTSCRNCWKNWRRSGSTFDIPGKTRRLNELDRELSDPELWNNPSRARQVTQEAGSLRRIVDGYRTLQ
SDANSLNEMLEMASDEERALLAEEQDTIQARVDELYRETLFTMKHADAPAIVRVKGGAGGTEAQDWAGMLARMYMRWAER
RGYKVDVLDEQPGEQAGYQSIEFIIRGEKAFGMMAPEHGVHRLVRVSPFDANNRRQTSFASVDVVPEVPEEQIDIVIPDS
DVRVDVYRSQGAGGQGVNTTDSAVRVTHLPTGIIVAIQVTRSQIKNREMAFQILKQRLYDLEMRKREEEEAKARGEQKKI
EWGSQIRSYVLDKQYVKDHRTGLMKHNPDDVLDGDLDDLMWAGLEWMAGKRAAEDGADEE
>Mature_379_residues
PAPRSTGETRKGDTSCRNCWKNWRRSGSTFDIPGKTRRLNELDRELSDPELWNNPSRARQVTQEAGSLRRIVDGYRTLQS
DANSLNEMLEMASDEERALLAEEQDTIQARVDELYRETLFTMKHADAPAIVRVKGGAGGTEAQDWAGMLARMYMRWAERR
GYKVDVLDEQPGEQAGYQSIEFIIRGEKAFGMMAPEHGVHRLVRVSPFDANNRRQTSFASVDVVPEVPEEQIDIVIPDSD
VRVDVYRSQGAGGQGVNTTDSAVRVTHLPTGIIVAIQVTRSQIKNREMAFQILKQRLYDLEMRKREEEEAKARGEQKKIE
WGSQIRSYVLDKQYVKDHRTGLMKHNPDDVLDGDLDDLMWAGLEWMAGKRAAEDGADEE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=304, Percent_Identity=33.5526315789474, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI34577120, Length=294, Percent_Identity=32.9931972789116, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI166795305, Length=202, Percent_Identity=33.6633663366337, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=44.5427728613569, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1787462, Length=236, Percent_Identity=41.5254237288136, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=217, Percent_Identity=35.0230414746544, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=33.6507936507937, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19921226, Length=285, Percent_Identity=32.9824561403509, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 43186; Mature: 43055

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPRSTGETRKGDTSCRNCWKNWRRSGSTFDIPGKTRRLNELDRELSDPELWNNPSRAR
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCHHCCCHHHHH
QVTQEAGSLRRIVDGYRTLQSDANSLNEMLEMASDEERALLAEEQDTIQARVDELYRETL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTMKHADAPAIVRVKGGAGGTEAQDWAGMLARMYMRWAERRGYKVDVLDEQPGEQAGYQS
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHCHHH
IEFIIRGEKAFGMMAPEHGVHRLVRVSPFDANNRRQTSFASVDVVPEVPEEQIDIVIPDS
HHHEEECCHHHCCCCCCHHHHHEEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCC
DVRVDVYRSQGAGGQGVNTTDSAVRVTHLPTGIIVAIQVTRSQIKNREMAFQILKQRLYD
CCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
LEMRKREEEEAKARGEQKKIEWGSQIRSYVLDKQYVKDHRTGLMKHNPDDVLDGDLDDLM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHH
WAGLEWMAGKRAAEDGADEE
HHHHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
PAPRSTGETRKGDTSCRNCWKNWRRSGSTFDIPGKTRRLNELDRELSDPELWNNPSRAR
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHCCCCHHCCCHHHHH
QVTQEAGSLRRIVDGYRTLQSDANSLNEMLEMASDEERALLAEEQDTIQARVDELYRETL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTMKHADAPAIVRVKGGAGGTEAQDWAGMLARMYMRWAERRGYKVDVLDEQPGEQAGYQS
HHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHCHHH
IEFIIRGEKAFGMMAPEHGVHRLVRVSPFDANNRRQTSFASVDVVPEVPEEQIDIVIPDS
HHHEEECCHHHCCCCCCHHHHHEEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCC
DVRVDVYRSQGAGGQGVNTTDSAVRVTHLPTGIIVAIQVTRSQIKNREMAFQILKQRLYD
CCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHH
LEMRKREEEEAKARGEQKKIEWGSQIRSYVLDKQYVKDHRTGLMKHNPDDVLDGDLDDLM
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHH
WAGLEWMAGKRAAEDGADEE
HHHHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA