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The map label for this gene is sucB [H]

Identifier: 94984247

GI number: 94984247

Start: 126791

End: 128068

Strand: Reverse

Name: sucB [H]

Synonym: Dgeo_0139

Alternate gene names: 94984247

Gene position: 128068-126791 (Counterclockwise)

Preceding gene: 94984248

Following gene: 94984245

Centisome position: 5.19

GC content: 64.16

Gene sequence:

>1278_bases
ATGGCCGAGATCAAGGTACCAGTCTTTTCCGAGTCGGTGAGCGAGGGCACGCTGCTGACCTGGCACAAGCAGCCCGGCGA
CGCCGTCAAGCGCGGCGAGGTTCTCGCAGAGATCGAAACCGACAAGGTGGTGCTCGAGGTCACTGCGCAGCAGGACGGCG
TCCTGACCTCCGTCACCAAGCATGAGGGGGATACGGTCCTCAGCGAGGAAGTGCTGGGCACCATCGGGGAAGCGGGAAGC
GCTCCCGCGGCCAGCACGCCGGCCCCTGCCCCGGACCAGGTCAGCGGCCCCGTTGCGGGCGAGGCCAGCGCGGGCGGCAC
GGCCGTTCAGGCTGACAGCACCGGTGTGCAGTCCGCTGCGAGCGTGGCCACTCGCCGAGACGACCTCTCTCCTGCTGTCC
GTAAGATTGTGGCGGAGCAGGGCCTTAACCCGGCGCAGATTCCTGCGACCGGACCGAAGGGGAACATCACCAAGGCAGAC
GCGCTTCAGGCAGCGACCAGCAGCCAGCCTGCGCCGGCTGCAGCTCCGGTGCAGGCTCCGCCCCCTCAAGCCGCCGCCGT
GCAGGTGCCGCAGGGAAACCGGCCCGAGCAGCGCGTCCCAATGACCCGAATCCGCCAACGAATCGCGGAGCGGCTGAAGG
AGGTCCAGAACACTGCCGCGATCCTGACCACCTTCAACGAGATCAACATGAAGCCCGCGATGGACCTGCGCAAGAAGTAT
CAGGACCAGTTCGTCGCCAAGCATGGCGTGAAGCTGGGCTTCATGAGCCTCTTCGTGCGCGCGGCCACCGAAGCCCTCAA
GCAGTTTCCAATCATCAATGCGAGTGTTGAGGGCAAGGACATCATCTACCACGGCTACTACGACATCGGCATCGCGGTGG
CCTCCGACCGCGGCCTGGTTGTGCCCATCTTGCGTGACACCGACCAGATGAGCCTCGCCGACATCGAGAAGCAGATCGCC
CAATTTGCCCAGAAGGCCAAGACCGGCAAGCTGACGCTGGAAGACATGAGCGGCGGCACCTTCTCCATCACCAACGGCGG
CACCTTTGGCTCGATGATGAGCACCCCGATCATCAACGCGCCGCAAAGCGCCATTCTGGGCATGCACAACATCATCGAGC
GCCCAGTGGCCGAGCAGGGCCAGTTTGTGATTCGCCCGATGATGTACGTCGCCCTCAGCTACGATCACCGCATTATTGAC
GGCCGCGAAGCCGTGCTGTTCCTGGTGGCGATCAAGAACGCTCTTGAAGACCCGGCGCGGATGCTGCTGGAGATTTAA

Upstream 100 bases:

>100_bases
GCTGAGGGGGCAGCCAGGGTGAAGGAGCCGGGAAAAACACCCGGTGCCTTCACCGTCACCGTGAGAGTCACGCCTCCGGA
GGGGTATAAAGGAACACGTT

Downstream 100 bases:

>100_bases
GAGCTTTCCACGTGCCCGCGGTCAGCTTTCCAGAGCGGAAAGCTGACCGCGGGCGCGTTTAGATCTTTTGCAATGGCCGC
GCCGCCAGCCATGCCGGTAC

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAEIKVPVFSESVSEGTLLTWHKQPGDAVKRGEVLAEIETDKVVLEVTAQQDGVLTSVTKHEGDTVLSEEVLGTIGEAGS
APAASTPAPAPDQVSGPVAGEASAGGTAVQADSTGVQSAASVATRRDDLSPAVRKIVAEQGLNPAQIPATGPKGNITKAD
ALQAATSSQPAPAAAPVQAPPPQAAAVQVPQGNRPEQRVPMTRIRQRIAERLKEVQNTAAILTTFNEINMKPAMDLRKKY
QDQFVAKHGVKLGFMSLFVRAATEALKQFPIINASVEGKDIIYHGYYDIGIAVASDRGLVVPILRDTDQMSLADIEKQIA
QFAQKAKTGKLTLEDMSGGTFSITNGGTFGSMMSTPIINAPQSAILGMHNIIERPVAEQGQFVIRPMMYVALSYDHRIID
GREAVLFLVAIKNALEDPARMLLEI

Sequences:

>Translated_425_residues
MAEIKVPVFSESVSEGTLLTWHKQPGDAVKRGEVLAEIETDKVVLEVTAQQDGVLTSVTKHEGDTVLSEEVLGTIGEAGS
APAASTPAPAPDQVSGPVAGEASAGGTAVQADSTGVQSAASVATRRDDLSPAVRKIVAEQGLNPAQIPATGPKGNITKAD
ALQAATSSQPAPAAAPVQAPPPQAAAVQVPQGNRPEQRVPMTRIRQRIAERLKEVQNTAAILTTFNEINMKPAMDLRKKY
QDQFVAKHGVKLGFMSLFVRAATEALKQFPIINASVEGKDIIYHGYYDIGIAVASDRGLVVPILRDTDQMSLADIEKQIA
QFAQKAKTGKLTLEDMSGGTFSITNGGTFGSMMSTPIINAPQSAILGMHNIIERPVAEQGQFVIRPMMYVALSYDHRIID
GREAVLFLVAIKNALEDPARMLLEI
>Mature_424_residues
AEIKVPVFSESVSEGTLLTWHKQPGDAVKRGEVLAEIETDKVVLEVTAQQDGVLTSVTKHEGDTVLSEEVLGTIGEAGSA
PAASTPAPAPDQVSGPVAGEASAGGTAVQADSTGVQSAASVATRRDDLSPAVRKIVAEQGLNPAQIPATGPKGNITKADA
LQAATSSQPAPAAAPVQAPPPQAAAVQVPQGNRPEQRVPMTRIRQRIAERLKEVQNTAAILTTFNEINMKPAMDLRKKYQ
DQFVAKHGVKLGFMSLFVRAATEALKQFPIINASVEGKDIIYHGYYDIGIAVASDRGLVVPILRDTDQMSLADIEKQIAQ
FAQKAKTGKLTLEDMSGGTFSITNGGTFGSMMSTPIINAPQSAILGMHNIIERPVAEQGQFVIRPMMYVALSYDHRIIDG
REAVLFLVAIKNALEDPARMLLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=30.6345733041575, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI203098816, Length=464, Percent_Identity=30.8189655172414, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI31711992, Length=444, Percent_Identity=28.6036036036036, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI110671329, Length=448, Percent_Identity=25.6696428571429, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.0248447204969, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=52.0094562647754, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1786305, Length=305, Percent_Identity=30.8196721311475, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=433, Percent_Identity=44.5727482678984, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=29.2410714285714, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=445, Percent_Identity=25.1685393258427, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538894, Length=336, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=420, Percent_Identity=44.2857142857143, Blast_Score=342, Evalue=9e-95,
Organism=Saccharomyces cerevisiae, GI6324258, Length=453, Percent_Identity=29.3598233995585, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=239, Percent_Identity=58.5774058577406, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=29.3859649122807, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=29.1304347826087, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45114; Mature: 44983

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKVPVFSESVSEGTLLTWHKQPGDAVKRGEVLAEIETDKVVLEVTAQQDGVLTSVTK
CCEEECCHHCCCCCCCCEEEEECCCCCHHHHCCEEEEECCCEEEEEEECCCCCCEEEEHH
HEGDTVLSEEVLGTIGEAGSAPAASTPAPAPDQVSGPVAGEASAGGTAVQADSTGVQSAA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHH
SVATRRDDLSPAVRKIVAEQGLNPAQIPATGPKGNITKADALQAATSSQPAPAAAPVQAP
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
PPQAAAVQVPQGNRPEQRVPMTRIRQRIAERLKEVQNTAAILTTFNEINMKPAMDLRKKY
CCCCCEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCHHHHHHHHH
QDQFVAKHGVKLGFMSLFVRAATEALKQFPIINASVEGKDIIYHGYYDIGIAVASDRGLV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEEECCCCEE
VPILRDTDQMSLADIEKQIAQFAQKAKTGKLTLEDMSGGTFSITNGGTFGSMMSTPIINA
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCCCCCCC
PQSAILGMHNIIERPVAEQGQFVIRPMMYVALSYDHRIIDGREAVLFLVAIKNALEDPAR
CHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHH
MLLEI
HHHCC
>Mature Secondary Structure 
AEIKVPVFSESVSEGTLLTWHKQPGDAVKRGEVLAEIETDKVVLEVTAQQDGVLTSVTK
CEEECCHHCCCCCCCCEEEEECCCCCHHHHCCEEEEECCCEEEEEEECCCCCCEEEEHH
HEGDTVLSEEVLGTIGEAGSAPAASTPAPAPDQVSGPVAGEASAGGTAVQADSTGVQSAA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHH
SVATRRDDLSPAVRKIVAEQGLNPAQIPATGPKGNITKADALQAATSSQPAPAAAPVQAP
HHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCC
PPQAAAVQVPQGNRPEQRVPMTRIRQRIAERLKEVQNTAAILTTFNEINMKPAMDLRKKY
CCCCCEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCHHHHHHHHH
QDQFVAKHGVKLGFMSLFVRAATEALKQFPIINASVEGKDIIYHGYYDIGIAVASDRGLV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEEECCCCEE
VPILRDTDQMSLADIEKQIAQFAQKAKTGKLTLEDMSGGTFSITNGGTFGSMMSTPIINA
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCHHHHCCCCCCC
PQSAILGMHNIIERPVAEQGQFVIRPMMYVALSYDHRIIDGREAVLFLVAIKNALEDPAR
CHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCHHH
MLLEI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]