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The map label for this gene is dusB [H]

Identifier: 94984185

GI number: 94984185

Start: 72893

End: 73894

Strand: Reverse

Name: dusB [H]

Synonym: Dgeo_0077

Alternate gene names: 94984185

Gene position: 73894-72893 (Counterclockwise)

Preceding gene: 94984188

Following gene: 94984184

Centisome position: 3.0

GC content: 69.86

Gene sequence:

>1002_bases
ATGATCTGCGGTCCCGGCTTCTACGCTCGCCGCCTGGCCCGGCCGGGGGCCGTCCTTGCCCCGATGGCGGGCTACAGCGA
CGCACCCATGCGCCAACTCGCCGCCGAGCAGGGGGCGCTGTGGACCGTCAGCGAGATGATCAGCGCGCGCGGTCTGGTGC
TGGGTGGCGACTCGGAAAAGCTCACGCTGGGGCGGCCCTATCCCGGCGAGGTCGGGCGGGTCGTTCAGCTCTTTGGTGCT
GAGCCCGACGTGCTGGCACAGGCGGTGGCCCGCGCTGAAAGCTGGTTTGCACCCGCCGCCCTGGACCTGAACATGGGCTG
CCCGGTCCCCAAGGTCAAGGGCCGCGGCGGCGCCTGTCTTCTCCAGACACCAGAAGTCGCCTACACGCTGGTGCGGGCCA
TGCGCTCGGCCACGACACTCGACGTGAGTGCCAAGATTCGCCTGGGCTGGGACACCGACCGCAGCGTGGAGATTGCGCAG
GGACTGGCGGCCGCGGGGGCAGCGCTGATCACCGTCCATGGACGGACCAGCGTGCAGCGCTACAGCGGTGAGGCAGACTG
GGACGCCATCGCCCGAGTCGCGGCCAGCGTGAAGGTGCCGGTGGTCGGCAGCGGCGACGTCAAAAGCGCCGAACAGGCTC
GTGCCCGCCTGAACACTGGGGTGGCCGCCGTGATGATTGGGCGCGGTGCGGTGGGAAATCCCTGGCTGTTTCGCGCGCTC
GCCAGCGGCGACGACGTGGTTCCCAGCGCGCAGGAGCGGGCCCGCACGGCGCTGCGCCACGCCCAGTTGCATGTCACCTT
CTACGGCCCCGACCGGTTCGGGCTGCTCAGCGTGCGCCCGTTGCGCAAGGTGTTGCCGCACTACCTGCCTGACCATCCCG
AGCTGCGCGCGGCACTGGTGCAGGTGAACACGGTGGCCGATGTGGAGCAGGCACTGGCGCCCCTGCTGGTTGACGCGCTC
CCGCCGCAGACTCAGAACTTCGTGCGGATGAGTGCCGAATAA

Upstream 100 bases:

>100_bases
GCAGCATACCGCGCCTGCTGCGGACTGGGCGTGAACGCCAGGTCTGCCGAATTGAGGCAAAATTCACGCAGTGGGGGGCA
GGCTACGCTAGGCTAGCGGG

Downstream 100 bases:

>100_bases
ACCACAAGCCGCCGCGCCCGAGCGGAGTATGCTGTGAGGCGGTCATGAACGTCCGCGAATATTATGCTTATCTTTCAGGT
GCGCGCGAGCAGCTCTGGAA

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MICGPGFYARRLARPGAVLAPMAGYSDAPMRQLAAEQGALWTVSEMISARGLVLGGDSEKLTLGRPYPGEVGRVVQLFGA
EPDVLAQAVARAESWFAPAALDLNMGCPVPKVKGRGGACLLQTPEVAYTLVRAMRSATTLDVSAKIRLGWDTDRSVEIAQ
GLAAAGAALITVHGRTSVQRYSGEADWDAIARVAASVKVPVVGSGDVKSAEQARARLNTGVAAVMIGRGAVGNPWLFRAL
ASGDDVVPSAQERARTALRHAQLHVTFYGPDRFGLLSVRPLRKVLPHYLPDHPELRAALVQVNTVADVEQALAPLLVDAL
PPQTQNFVRMSAE

Sequences:

>Translated_333_residues
MICGPGFYARRLARPGAVLAPMAGYSDAPMRQLAAEQGALWTVSEMISARGLVLGGDSEKLTLGRPYPGEVGRVVQLFGA
EPDVLAQAVARAESWFAPAALDLNMGCPVPKVKGRGGACLLQTPEVAYTLVRAMRSATTLDVSAKIRLGWDTDRSVEIAQ
GLAAAGAALITVHGRTSVQRYSGEADWDAIARVAASVKVPVVGSGDVKSAEQARARLNTGVAAVMIGRGAVGNPWLFRAL
ASGDDVVPSAQERARTALRHAQLHVTFYGPDRFGLLSVRPLRKVLPHYLPDHPELRAALVQVNTVADVEQALAPLLVDAL
PPQTQNFVRMSAE
>Mature_333_residues
MICGPGFYARRLARPGAVLAPMAGYSDAPMRQLAAEQGALWTVSEMISARGLVLGGDSEKLTLGRPYPGEVGRVVQLFGA
EPDVLAQAVARAESWFAPAALDLNMGCPVPKVKGRGGACLLQTPEVAYTLVRAMRSATTLDVSAKIRLGWDTDRSVEIAQ
GLAAAGAALITVHGRTSVQRYSGEADWDAIARVAASVKVPVVGSGDVKSAEQARARLNTGVAAVMIGRGAVGNPWLFRAL
ASGDDVVPSAQERARTALRHAQLHVTFYGPDRFGLLSVRPLRKVLPHYLPDHPELRAALVQVNTVADVEQALAPLLVDAL
PPQTQNFVRMSAE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=282, Percent_Identity=31.5602836879433, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI239788462, Length=267, Percent_Identity=32.2097378277154, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI40807366, Length=317, Percent_Identity=31.2302839116719, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI31742496, Length=240, Percent_Identity=32.0833333333333, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI8923374, Length=288, Percent_Identity=26.7361111111111, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789660, Length=325, Percent_Identity=35.0769230769231, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788462, Length=246, Percent_Identity=37.3983739837398, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17543114, Length=271, Percent_Identity=30.9963099630996, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=315, Percent_Identity=28.8888888888889, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=223, Percent_Identity=32.2869955156951, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=248, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=295, Percent_Identity=28.135593220339, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=312, Percent_Identity=25.9615384615385, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=242, Percent_Identity=29.7520661157025, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585320, Length=289, Percent_Identity=31.1418685121107, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24580595, Length=265, Percent_Identity=29.811320754717, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920448, Length=265, Percent_Identity=29.811320754717, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19921524, Length=239, Percent_Identity=29.2887029288703, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45549423, Length=244, Percent_Identity=30.327868852459, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35283; Mature: 35283

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICGPGFYARRLARPGAVLAPMAGYSDAPMRQLAAEQGALWTVSEMISARGLVLGGDSEK
CCCCCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCEEEHHHHHHHCCEEEECCCCE
LTLGRPYPGEVGRVVQLFGAEPDVLAQAVARAESWFAPAALDLNMGCPVPKVKGRGGACL
EEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEE
LQTPEVAYTLVRAMRSATTLDVSAKIRLGWDTDRSVEIAQGLAAAGAALITVHGRTSVQR
EECHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHCCEEEEEEECCCHHHH
YSGEADWDAIARVAASVKVPVVGSGDVKSAEQARARLNTGVAAVMIGRGAVGNPWLFRAL
CCCCCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
ASGDDVVPSAQERARTALRHAQLHVTFYGPDRFGLLSVRPLRKVLPHYLPDHPELRAALV
HCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCEEECHHHHHHHHHHCCCCHHHHHHHH
QVNTVADVEQALAPLLVDALPPQTQNFVRMSAE
HHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCC
>Mature Secondary Structure
MICGPGFYARRLARPGAVLAPMAGYSDAPMRQLAAEQGALWTVSEMISARGLVLGGDSEK
CCCCCCHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCEEEHHHHHHHCCEEEECCCCE
LTLGRPYPGEVGRVVQLFGAEPDVLAQAVARAESWFAPAALDLNMGCPVPKVKGRGGACL
EEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEE
LQTPEVAYTLVRAMRSATTLDVSAKIRLGWDTDRSVEIAQGLAAAGAALITVHGRTSVQR
EECHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCHHHHHHHHHCCEEEEEEECCCHHHH
YSGEADWDAIARVAASVKVPVVGSGDVKSAEQARARLNTGVAAVMIGRGAVGNPWLFRAL
CCCCCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHH
ASGDDVVPSAQERARTALRHAQLHVTFYGPDRFGLLSVRPLRKVLPHYLPDHPELRAALV
HCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCCEEECHHHHHHHHHHCCCCHHHHHHHH
QVNTVADVEQALAPLLVDALPPQTQNFVRMSAE
HHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]