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The map label for this gene is degA [H]

Identifier: 94972164

GI number: 94972164

Start: 331100

End: 332119

Strand: Direct

Name: degA [H]

Synonym: Dgeo_2697

Alternate gene names: 94972164

Gene position: 331100-332119 (Clockwise)

Preceding gene: 94972165

Following gene: 94972163

Centisome position: 57.67

GC content: 65.39

Gene sequence:

>1020_bases
ATGCGTTCCAATCCTGTGACCCTTGCTGACATCGCCAGTCAGGCAGGCGTATCAAAAATGACGGTCTCCAAAGTTGTGAA
CAAGCAGCCCGGCATCTCTGAGGCGACCCGGCAGCGGGTATTGCGGATCATCGAGGAACTAGGATATACGGCCCACCCCT
CAGCCCGGGCATTGGCCGGAGGGCGCACGAACACCCTCGGAATTGTCGTGCCCTCCATCGGCCCCCAGTACATTAGCGAG
GTGGTGCGCGGCGCCGACCTCGTTGCTTATGAGGCTGGTCTGGACCTTTTGATCTCCACCACCCAGGAGGATACTTCCCA
CGAACGGCAGAACGTTGGACGCCTCTCTCGTGGTCTGGTAGACGGCCTGCTGATGGTTCTGCCCCGGTCGCTCGACCGCT
ATGCCGACGCGCTCAAGCAGGCCGAGGTGCCCGTCGTCGTTGTCGCGTCGGCCAACACCACCATGCCGTTCCCCCTAGTT
GACGCCGATCACTATCACGGCGCTCGGCTCGCCGTCGCCCATCTGCTCGGCCTGGGGCACCGGCGAGTCGGCTTCATCGC
GGGCCGTCGGGACACCACCGCGAGCCTTGAACGCCTGCGCGGCTACCGCGAGGGACTCCTCACCGCCGGGCTTACCCCCG
ACCCCGTCCTCATCCGGCCTGGCGAGTACACCCAGCCAGGCGGCTTCGCTGCTGCGCGGTCCCTGCTCGACTTGCCCGAC
CCACCCACGGCGATCTTCGCAGCCAACGACCTCTCCGCCTTTGGAGCGATGGAGGCGATCAAGGACCGGGGACTGCGTGT
TCCGGATGACGTCTCCGTGGTCGGCTTCGACGATATTCCCCAAGCCAACCAGGTCCACCCGGCCCTGACCACCGTGCGCC
AACCCCTCGTTGAAATGGGCACCGCCGGGACTCGGCAACTCCTGAACCTTCTGCACGGCGTCGGACCGGTCACCGACCGT
CTCGTGCTGCCCACCGAATTGATCGTCCGCGCCTCCACCGGTCCAGCTCCCCGAACCTGA

Upstream 100 bases:

>100_bases
CAGGACAGGTGGAGGACAACGCCGCCGAAACCCTGTTGACGTTCCACAAGGATAAGCGTAAAGTTACCGGTAACCAAAAA
CCTGGAAGAAGGAGGTGGGG

Downstream 100 bases:

>100_bases
CGCTCACCGCTGCCCCGAAGAAAGGCGGGGCTCCTCTAGGAAGCCACCCTTCATTCCACCGCCACTACCCGGCGACGAAC
GTGCTCCCCACGTTCGCGGG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRSNPVTLADIASQAGVSKMTVSKVVNKQPGISEATRQRVLRIIEELGYTAHPSARALAGGRTNTLGIVVPSIGPQYISE
VVRGADLVAYEAGLDLLISTTQEDTSHERQNVGRLSRGLVDGLLMVLPRSLDRYADALKQAEVPVVVVASANTTMPFPLV
DADHYHGARLAVAHLLGLGHRRVGFIAGRRDTTASLERLRGYREGLLTAGLTPDPVLIRPGEYTQPGGFAAARSLLDLPD
PPTAIFAANDLSAFGAMEAIKDRGLRVPDDVSVVGFDDIPQANQVHPALTTVRQPLVEMGTAGTRQLLNLLHGVGPVTDR
LVLPTELIVRASTGPAPRT

Sequences:

>Translated_339_residues
MRSNPVTLADIASQAGVSKMTVSKVVNKQPGISEATRQRVLRIIEELGYTAHPSARALAGGRTNTLGIVVPSIGPQYISE
VVRGADLVAYEAGLDLLISTTQEDTSHERQNVGRLSRGLVDGLLMVLPRSLDRYADALKQAEVPVVVVASANTTMPFPLV
DADHYHGARLAVAHLLGLGHRRVGFIAGRRDTTASLERLRGYREGLLTAGLTPDPVLIRPGEYTQPGGFAAARSLLDLPD
PPTAIFAANDLSAFGAMEAIKDRGLRVPDDVSVVGFDDIPQANQVHPALTTVRQPLVEMGTAGTRQLLNLLHGVGPVTDR
LVLPTELIVRASTGPAPRT
>Mature_339_residues
MRSNPVTLADIASQAGVSKMTVSKVVNKQPGISEATRQRVLRIIEELGYTAHPSARALAGGRTNTLGIVVPSIGPQYISE
VVRGADLVAYEAGLDLLISTTQEDTSHERQNVGRLSRGLVDGLLMVLPRSLDRYADALKQAEVPVVVVASANTTMPFPLV
DADHYHGARLAVAHLLGLGHRRVGFIAGRRDTTASLERLRGYREGLLTAGLTPDPVLIRPGEYTQPGGFAAARSLLDLPD
PPTAIFAANDLSAFGAMEAIKDRGLRVPDDVSVVGFDDIPQANQVHPALTTVRQPLVEMGTAGTRQLLNLLHGVGPVTDR
LVLPTELIVRASTGPAPRT

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=332, Percent_Identity=34.0361445783133, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789068, Length=311, Percent_Identity=35.048231511254, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1790194, Length=296, Percent_Identity=35.472972972973, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789202, Length=301, Percent_Identity=35.2159468438538, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1788474, Length=324, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1787948, Length=305, Percent_Identity=29.8360655737705, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=33.0383480825959, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=26.6666666666667, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994940, Length=313, Percent_Identity=25.8785942492013, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790715, Length=309, Percent_Identity=27.1844660194175, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789456, Length=309, Percent_Identity=28.8025889967638, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787906, Length=344, Percent_Identity=25, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790689, Length=311, Percent_Identity=25.08038585209, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790192, Length=253, Percent_Identity=26.4822134387352, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786268, Length=284, Percent_Identity=22.887323943662, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36077; Mature: 36077

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSNPVTLADIASQAGVSKMTVSKVVNKQPGISEATRQRVLRIIEELGYTAHPSARALAG
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCC
GRTNTLGIVVPSIGPQYISEVVRGADLVAYEAGLDLLISTTQEDTSHERQNVGRLSRGLV
CCCCEEEEEECCCCHHHHHHHHCCCHHEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHH
DGLLMVLPRSLDRYADALKQAEVPVVVVASANTTMPFPLVDADHYHGARLAVAHLLGLGH
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
RRVGFIAGRRDTTASLERLRGYREGLLTAGLTPDPVLIRPGEYTQPGGFAAARSLLDLPD
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCC
PPTAIFAANDLSAFGAMEAIKDRGLRVPDDVSVVGFDDIPQANQVHPALTTVRQPLVEMG
CCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHC
TAGTRQLLNLLHGVGPVTDRLVLPTELIVRASTGPAPRT
CCCHHHHHHHHHCCCCHHCCEECCHHHEEECCCCCCCCC
>Mature Secondary Structure
MRSNPVTLADIASQAGVSKMTVSKVVNKQPGISEATRQRVLRIIEELGYTAHPSARALAG
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCC
GRTNTLGIVVPSIGPQYISEVVRGADLVAYEAGLDLLISTTQEDTSHERQNVGRLSRGLV
CCCCEEEEEECCCCHHHHHHHHCCCHHEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHH
DGLLMVLPRSLDRYADALKQAEVPVVVVASANTTMPFPLVDADHYHGARLAVAHLLGLGH
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
RRVGFIAGRRDTTASLERLRGYREGLLTAGLTPDPVLIRPGEYTQPGGFAAARSLLDLPD
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCC
PPTAIFAANDLSAFGAMEAIKDRGLRVPDDVSVVGFDDIPQANQVHPALTTVRQPLVEMG
CCCEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHC
TAGTRQLLNLLHGVGPVTDRLVLPTELIVRASTGPAPRT
CCCHHHHHHHHHCCCCHHCCEECCHHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]