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The map label for this gene is cobS [H]

Identifier: 94971836

GI number: 94971836

Start: 122647

End: 123387

Strand: Direct

Name: cobS [H]

Synonym: Dgeo_2367

Alternate gene names: 94971836

Gene position: 122647-123387 (Clockwise)

Preceding gene: 94971838

Following gene: 94971835

Centisome position: 21.36

GC content: 71.52

Gene sequence:

>741_bases
GTGACTGATCCGCGTTCCCGCTGGCAACGCCAACTTGGCGCCGCGCACCTCGCCCTGACTTTCCTGACCACGCTGCCCCT
CCCCCACGTCGCCCAGGTCCGGGACGGCGATTTTGCGCGGGCGAGCGCCTTTTATCCGCTGGCCGGGTACGTGGTGGGTG
GCCTCGTTGCGGCGCTGCTGGCGCTGCCCTTGCCCCTCCCGGATGGCGTGCATGCGGCGCTCGCGGTGGCTGCCTGGCTG
GCGCTCACCGGGATGCTGCACTTCGACGGCCTGGTGGATTGTGCCGACGCCCTGTTCGCTGTCAAAAGCCCCGCTCGGCG
GCTGGAGATCCTGCGTGACGTGCACGTGGGCGCCTTTGGTCTCGCCAGCGGTGTGCTTGCCCTGCTGTTGCTCTGGAGCC
TGCTGGCTGCGCCCCTCCCTCTTCATGCTCCGCTGGTCGCGGCAGTGGTCGCGCGGCTGCTGGTCCTGCTGCCCATGAAC
CTGTATCCGGCGGCGCGGCCGGAGTCGCTGGGTGCTCGCTCGCGGGAAGGCCGCTTGCTCCCCGCGCTGCTGCTCGCCCT
GCCGGCCCTGCTGCTCCCGGGGGCCTGGTTGGCCGCGCTGGTTGCGCTCGTTGGCGTGCTGCTGGTGGCCCGCTTCGCAG
CCGCGCGACTGGGTGGCGGCCTGACTGGGGACATCTACGGCCTGCTGATCGTCACGGCGGAGCTGCTCGCCCTGTGTGCC
TACGCCTGGGGCCGCGGCTGA

Upstream 100 bases:

>100_bases
GATTTGGCTCCCCGCCGATTCGGTATGAGAAGCCGAGTATGCCGGGTTTGGTCTCTGCCCGGCCTCCCCAGGGATCTCGC
CAGCCTGGCATGATCTGCCC

Downstream 100 bases:

>100_bases
TGCTGACCCTCTATCTCGTTCGGCACGCGCCCACCCGCCCCAACGCTGAGCGGCGCTACCCCCACTGGGACGAGGACGCC
CCGCTTTCTGGGGCCGGTCG

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTDPRSRWQRQLGAAHLALTFLTTLPLPHVAQVRDGDFARASAFYPLAGYVVGGLVAALLALPLPLPDGVHAALAVAAWL
ALTGMLHFDGLVDCADALFAVKSPARRLEILRDVHVGAFGLASGVLALLLLWSLLAAPLPLHAPLVAAVVARLLVLLPMN
LYPAARPESLGARSREGRLLPALLLALPALLLPGAWLAALVALVGVLLVARFAAARLGGGLTGDIYGLLIVTAELLALCA
YAWGRG

Sequences:

>Translated_246_residues
MTDPRSRWQRQLGAAHLALTFLTTLPLPHVAQVRDGDFARASAFYPLAGYVVGGLVAALLALPLPLPDGVHAALAVAAWL
ALTGMLHFDGLVDCADALFAVKSPARRLEILRDVHVGAFGLASGVLALLLLWSLLAAPLPLHAPLVAAVVARLLVLLPMN
LYPAARPESLGARSREGRLLPALLLALPALLLPGAWLAALVALVGVLLVARFAAARLGGGLTGDIYGLLIVTAELLALCA
YAWGRG
>Mature_245_residues
TDPRSRWQRQLGAAHLALTFLTTLPLPHVAQVRDGDFARASAFYPLAGYVVGGLVAALLALPLPLPDGVHAALAVAAWLA
LTGMLHFDGLVDCADALFAVKSPARRLEILRDVHVGAFGLASGVLALLLLWSLLAAPLPLHAPLVAAVVARLLVLLPMNL
YPAARPESLGARSREGRLLPALLLALPALLLPGAWLAALVALVGVLLVARFAAARLGGGLTGDIYGLLIVTAELLALCAY
AWGRG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=240, Percent_Identity=33.75, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25629; Mature: 25498

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPRSRWQRQLGAAHLALTFLTTLPLPHVAQVRDGDFARASAFYPLAGYVVGGLVAALL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPLPLPDGVHAALAVAAWLALTGMLHFDGLVDCADALFAVKSPARRLEILRDVHVGAFG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LASGVLALLLLWSLLAAPLPLHAPLVAAVVARLLVLLPMNLYPAARPESLGARSREGRLL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCHH
PALLLALPALLLPGAWLAALVALVGVLLVARFAAARLGGGLTGDIYGLLIVTAELLALCA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YAWGRG
HHHCCC
>Mature Secondary Structure 
TDPRSRWQRQLGAAHLALTFLTTLPLPHVAQVRDGDFARASAFYPLAGYVVGGLVAALL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPLPLPDGVHAALAVAAWLALTGMLHFDGLVDCADALFAVKSPARRLEILRDVHVGAFG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LASGVLALLLLWSLLAAPLPLHAPLVAAVVARLLVLLPMNLYPAARPESLGARSREGRLL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCHH
PALLLALPALLLPGAWLAALVALVGVLLVARFAAARLGGGLTGDIYGLLIVTAELLALCA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YAWGRG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]