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The map label for this gene is kgtP [H]

Identifier: 94314825

GI number: 94314825

Start: 106122

End: 107435

Strand: Reverse

Name: kgtP [H]

Synonym: Rmet_5906

Alternate gene names: 94314825

Gene position: 107435-106122 (Counterclockwise)

Preceding gene: 94314829

Following gene: 94314824

Centisome position: 4.16

GC content: 63.77

Gene sequence:

>1314_bases
ATGACAGACACCGCCTCTTCCGTCACCACCGGTACGGCACCCTCCCCAGAAGAAGTCAAAAAACGCATCTTCGCGATTGT
TGCCGCGTCGTCCGGCAATCTGGTGGAATGGTTCGACTTTTATGTCTATGCGTTCAGCGCGATCTATTTCGCGTCATCGT
TCTTCCCCAAAGCCGACCCCACCGCGCAGCTCCTGAACACCGCCGGGGTGTTCGCGGCCGGCTTCCTGATGCGGCCGATC
GGCGGATGGCTATTCGGCCGCATTGCCGATCGCACCGGCCGCAAGCACTCGATGGTGATCTCGGTCACGATGATGTGCTT
CGGCTCGCTGATGATCGCCTGCCTGCCGACCTATGCCGCGATCGGCAACCTGGCGCCAGCGCTTCTGCTGCTGGCCCGGC
TGCTGCAGGGCCTCTCGGTCGGTGGCGAGTACGGCACTACCGCCACGTACATGAGCGAAGTGGCGCTCAAGGGCCGGCGC
GGCTTCTTCTCATCGTTCCAGTACGTGACCCTGATCGGCGGTCAGCTGCTGGCCGTACTGGTGATCGTGATCCTGGAGCA
GTTGCTGACCGAGGCCGAACTGAAGGCCTGGGGCTGGCGCATCCCGTTCGTGGTTGGCGCGGTCACCGCGGTGGTGGCCC
TGCTGCTGCGCCGCACGCTGCATGAAACGACCACCGCCCAGTCGCGCAACAGCAAAGGCGCCGGCACCGTGGGCGAGCTC
TTCCGCCACCACAAGGCCGCGTTCTTCACGGTGCTCGGGTACACGGCCGGCGGCTCGCTGATCTTCTACACGTTCACCAC
GTACATGCAGAAGTACCTGGTCAACACGGCCGGCATGCCGATCAAGACGGCCAGCTACGTGATGACAGGCTGCCTGTTCC
TGTACATGTGCATGCAGCCGCTGTTCGGCATGTTGTCGGACCGCATCGGTCGCCGCAACAACATGCTGATGTTCGGCGCG
CTGGGCGCAGTCGCCACAGTGCCGATCCTGACCGCGCTCAAGACCGTCAGCAGCCCCGAGATGGCGCTGTTGCTGATCTG
CGTGGCGCTGGCCATCGTCAGCTTCTACACGTCGATCAGCGGCATCGTGAAGGCCGAGATGTTCCCGCCGGAAGTGCGTG
CGCTGGGCGTCGGCCTGGCATACGCGGTCGCCAACGCCATTTTTGGCGGCTCGGCCGAATATGTGGCGCTGGGCCTGAAA
TCGATCGGGCACGAGAGCGCGTTCTACTGGTATGTCACCGCGATGATGGTGGTGGCGTTCCTGGTCAGCCTGCGCCTGCC
GCGCCAGGCCAAGTACCTGCATCACGAGCACTGA

Upstream 100 bases:

>100_bases
GTGTGCGACGTCGCTCCCGCCACGAACCGCCTGGCCTCGTCGCCCATTGCGCCCGCCCCCATCCGGCGGCAAGCCCCACC
GCCAAGAATCAGAAGGTCCT

Downstream 100 bases:

>100_bases
CCGGCCACCCGCCGCAGTCATGGCAAACTGAGGGGCCTTCGGGCCCCTTTTTATTTGCACGCGCCCTTCGCTCTCCTCCC
CGCCATGGATCTCAACGCCC

Product: alpha-ketoglutarate transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTDTASSVTTGTAPSPEEVKKRIFAIVAASSGNLVEWFDFYVYAFSAIYFASSFFPKADPTAQLLNTAGVFAAGFLMRPI
GGWLFGRIADRTGRKHSMVISVTMMCFGSLMIACLPTYAAIGNLAPALLLLARLLQGLSVGGEYGTTATYMSEVALKGRR
GFFSSFQYVTLIGGQLLAVLVIVILEQLLTEAELKAWGWRIPFVVGAVTAVVALLLRRTLHETTTAQSRNSKGAGTVGEL
FRHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMPIKTASYVMTGCLFLYMCMQPLFGMLSDRIGRRNNMLMFGA
LGAVATVPILTALKTVSSPEMALLLICVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVALGLK
SIGHESAFYWYVTAMMVVAFLVSLRLPRQAKYLHHEH

Sequences:

>Translated_437_residues
MTDTASSVTTGTAPSPEEVKKRIFAIVAASSGNLVEWFDFYVYAFSAIYFASSFFPKADPTAQLLNTAGVFAAGFLMRPI
GGWLFGRIADRTGRKHSMVISVTMMCFGSLMIACLPTYAAIGNLAPALLLLARLLQGLSVGGEYGTTATYMSEVALKGRR
GFFSSFQYVTLIGGQLLAVLVIVILEQLLTEAELKAWGWRIPFVVGAVTAVVALLLRRTLHETTTAQSRNSKGAGTVGEL
FRHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMPIKTASYVMTGCLFLYMCMQPLFGMLSDRIGRRNNMLMFGA
LGAVATVPILTALKTVSSPEMALLLICVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVALGLK
SIGHESAFYWYVTAMMVVAFLVSLRLPRQAKYLHHEH
>Mature_436_residues
TDTASSVTTGTAPSPEEVKKRIFAIVAASSGNLVEWFDFYVYAFSAIYFASSFFPKADPTAQLLNTAGVFAAGFLMRPIG
GWLFGRIADRTGRKHSMVISVTMMCFGSLMIACLPTYAAIGNLAPALLLLARLLQGLSVGGEYGTTATYMSEVALKGRRG
FFSSFQYVTLIGGQLLAVLVIVILEQLLTEAELKAWGWRIPFVVGAVTAVVALLLRRTLHETTTAQSRNSKGAGTVGELF
RHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMPIKTASYVMTGCLFLYMCMQPLFGMLSDRIGRRNNMLMFGAL
GAVATVPILTALKTVSSPEMALLLICVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVALGLKS
IGHESAFYWYVTAMMVVAFLVSLRLPRQAKYLHHEH

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=68.7793427230047, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=29.2161520190024, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1788292, Length=383, Percent_Identity=30.2872062663185, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789941, Length=447, Percent_Identity=31.0961968680089, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082231, Length=321, Percent_Identity=25.5451713395639, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082404, Length=376, Percent_Identity=23.936170212766, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47270; Mature: 47139

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCHHHHHHCCCC
>Mature Secondary Structure 
TDTASSVTTGTAPSPEEVKKRIFAIVAASSGNLVEWFDFYVYAFSAIYFASSFFPKADP
CCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC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HHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]