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The map label for this gene is eutB [H]

Identifier: 94314821

GI number: 94314821

Start: 102387

End: 103769

Strand: Direct

Name: eutB [H]

Synonym: Rmet_5902

Alternate gene names: 94314821

Gene position: 102387-103769 (Clockwise)

Preceding gene: 94314819

Following gene: 94314822

Centisome position: 3.97

GC content: 67.25

Gene sequence:

>1383_bases
ATGGCCTTCACGCATACCGTCGGCGTACGCCGACACGTCTTTGACGATCTGCGCACGCTGCTGGCCAAGGCCAGTCCGGC
CCGGTCGGGCGACGCCCTCGCGGGTATCGCCGCCGCCAGCGAAGAAGAGCGGATGGCGGCGCGTATGGCGCTGGCGGACA
TGCCGCTGGCGCATTGCCTTGCGGAGCCGCTGGTTCCTTACGAGGCCGACGAGGTCACCCGGCTGATCGTGGACAGCCAC
GACGCGCAGGCGTTCGCGGCGCTTGCCAGCCTGACCGTCGGGGAGTTCCGGGACTGGCTGCTGCGGCACGACACCGATAC
GGCCACGCTGGCGCGGGTGGCGCCAGGCGTCACCCCCGAGATGGCGGCGGCCGTGAGCAAGCTCATGCGCAACCAGGACC
TGGTGGCCGTGGCGCGCAAATGCGCGGTGGTCACCGCCTTCCGCAACACCATCGGGCTGCCCGGGCGCCTATCCGTGCGG
CTGCAGCCGAACCATCCGACCGACGACCCGCGCGGCATCGCCGCATCGATCATCGACGGGCTGCTGTTCGGCTGCGGCGA
TGCCACCATCGGCATCAACCCGGCCAGCGACAACCTTGGGGCGATCACCACGTTGCTGAAGCTTCTGGATGACATTCGCA
TCCGCTACGAGATACCCACCCAGTCTTGCGTGCTGACTCACGTCACCAACACCTTGCGCGTGATGGAGCTGGGCGCGCCG
GTCGATTTGGTGTTCCAGTCGGTGGCTGGCAGCCAGGCGGCCAATGCAGCGTTCGGCATCTCTCTGGCGCTGCTCGAAGA
GGCACGGCAGGCGGCGTTGTCGCTGCGGCGCGGCACGGTGGGGCACAACGTGATGTATTTCGAGACCGGCCAGGGCAGCG
CGCTTTCCGCCAACGCCCACCATGGTGTGGATCAGCAGACCATGGAGGCGCGCGCGTATGCGGTGGCGCGCCGGTTCGAG
CCGTTGCTGGTCAATACCGTGGTGGGGTTCATCGGTCCCGAGTATCTCTACGACGGCAAGCAGATCATCCGGGCCGGGCT
TGAGGACCACTTCTGCGGCAAGCTGCTGGGCGTGCCGATGGGCTGCGACGTCTGTTACACGAACCACGCCGAGGCCGATC
AGGACGACATGGACAACCTGCTGACGCTGTTTGGCGTGGCGGGCATCAACTTCATCATGGGCGTGCCCGGCGCCGACGAC
ATCATGCTGAACTACCAGAGCACGTCGTTTCATGACGCGCTGTACCTGCGCGAAGTCCTTGGCCTGCGGCCCGCGCCGGA
GTTCGAGGCGTGGCTGCGCAAGATGGGCATTGTCGACGCGGGCGGGCGGTTGCTGGAGCCTGGCACGCGCCAGGCGCTGC
TGACCATGGCGGACACGCTTTGA

Upstream 100 bases:

>100_bases
CTGATAACGGGACAAATCGTGCGGACGGTTGCCCGGGCGTGATATTTGCAGCACTGGCCTATATTGAAGTTCCGTGGCAG
CCCTTCAGGAGACCACTTGC

Downstream 100 bases:

>100_bases
ACGGGAGGAGGAACACCATGTCGACGCCAGCGAACCCCGAAGACCCGTGGGCGCGCTTGCGCCAGTTCACACGCGCCCGC
ATCGCCATCGGCCGCACCGG

Product: ethanolamine ammonia lyase large subunit

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAFTHTVGVRRHVFDDLRTLLAKASPARSGDALAGIAAASEEERMAARMALADMPLAHCLAEPLVPYEADEVTRLIVDSH
DAQAFAALASLTVGEFRDWLLRHDTDTATLARVAPGVTPEMAAAVSKLMRNQDLVAVARKCAVVTAFRNTIGLPGRLSVR
LQPNHPTDDPRGIAASIIDGLLFGCGDATIGINPASDNLGAITTLLKLLDDIRIRYEIPTQSCVLTHVTNTLRVMELGAP
VDLVFQSVAGSQAANAAFGISLALLEEARQAALSLRRGTVGHNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFE
PLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDNLLTLFGVAGINFIMGVPGADD
IMLNYQSTSFHDALYLREVLGLRPAPEFEAWLRKMGIVDAGGRLLEPGTRQALLTMADTL

Sequences:

>Translated_460_residues
MAFTHTVGVRRHVFDDLRTLLAKASPARSGDALAGIAAASEEERMAARMALADMPLAHCLAEPLVPYEADEVTRLIVDSH
DAQAFAALASLTVGEFRDWLLRHDTDTATLARVAPGVTPEMAAAVSKLMRNQDLVAVARKCAVVTAFRNTIGLPGRLSVR
LQPNHPTDDPRGIAASIIDGLLFGCGDATIGINPASDNLGAITTLLKLLDDIRIRYEIPTQSCVLTHVTNTLRVMELGAP
VDLVFQSVAGSQAANAAFGISLALLEEARQAALSLRRGTVGHNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFE
PLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDNLLTLFGVAGINFIMGVPGADD
IMLNYQSTSFHDALYLREVLGLRPAPEFEAWLRKMGIVDAGGRLLEPGTRQALLTMADTL
>Mature_459_residues
AFTHTVGVRRHVFDDLRTLLAKASPARSGDALAGIAAASEEERMAARMALADMPLAHCLAEPLVPYEADEVTRLIVDSHD
AQAFAALASLTVGEFRDWLLRHDTDTATLARVAPGVTPEMAAAVSKLMRNQDLVAVARKCAVVTAFRNTIGLPGRLSVRL
QPNHPTDDPRGIAASIIDGLLFGCGDATIGINPASDNLGAITTLLKLLDDIRIRYEIPTQSCVLTHVTNTLRVMELGAPV
DLVFQSVAGSQAANAAFGISLALLEEARQAALSLRRGTVGHNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFEP
LLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDNLLTLFGVAGINFIMGVPGADDI
MLNYQSTSFHDALYLREVLGLRPAPEFEAWLRKMGIVDAGGRLLEPGTRQALLTMADTL

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=433, Percent_Identity=50.8083140877598, Blast_Score=456, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 49413; Mature: 49282

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTHTVGVRRHVFDDLRTLLAKASPARSGDALAGIAAASEEERMAARMALADMPLAHCL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCHHHHHH
AEPLVPYEADEVTRLIVDSHDAQAFAALASLTVGEFRDWLLRHDTDTATLARVAPGVTPE
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHH
MAAAVSKLMRNQDLVAVARKCAVVTAFRNTIGLPGRLSVRLQPNHPTDDPRGIAASIIDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
LLFGCGDATIGINPASDNLGAITTLLKLLDDIRIRYEIPTQSCVLTHVTNTLRVMELGAP
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHEEECCCC
VDLVFQSVAGSQAANAAFGISLALLEEARQAALSLRRGTVGHNVMYFETGQGSALSANAH
HHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCC
HGVDQQTMEARAYAVARRFEPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPM
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCC
GCDVCYTNHAEADQDDMDNLLTLFGVAGINFIMGVPGADDIMLNYQSTSFHDALYLREVL
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
GLRPAPEFEAWLRKMGIVDAGGRLLEPGTRQALLTMADTL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AFTHTVGVRRHVFDDLRTLLAKASPARSGDALAGIAAASEEERMAARMALADMPLAHCL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCHHHHHH
AEPLVPYEADEVTRLIVDSHDAQAFAALASLTVGEFRDWLLRHDTDTATLARVAPGVTPE
HCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHH
MAAAVSKLMRNQDLVAVARKCAVVTAFRNTIGLPGRLSVRLQPNHPTDDPRGIAASIIDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHH
LLFGCGDATIGINPASDNLGAITTLLKLLDDIRIRYEIPTQSCVLTHVTNTLRVMELGAP
HHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHEEECCCC
VDLVFQSVAGSQAANAAFGISLALLEEARQAALSLRRGTVGHNVMYFETGQGSALSANAH
HHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEECCCC
HGVDQQTMEARAYAVARRFEPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPM
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHCCHHHHHHCCCHHHHHHHHCCCC
GCDVCYTNHAEADQDDMDNLLTLFGVAGINFIMGVPGADDIMLNYQSTSFHDALYLREVL
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
GLRPAPEFEAWLRKMGIVDAGGRLLEPGTRQALLTMADTL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]