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The map label for this gene is leuA1 [H]

Identifier: 94314806

GI number: 94314806

Start: 83541

End: 85244

Strand: Reverse

Name: leuA1 [H]

Synonym: Rmet_5887

Alternate gene names: 94314806

Gene position: 85244-83541 (Counterclockwise)

Preceding gene: 291481474

Following gene: 94314805

Centisome position: 3.3

GC content: 63.79

Gene sequence:

>1704_bases
ATGCCCATGTTGCAGGATCCATCCACCAAATATCGCCCCGCCACCACCGTCGACTTGCATGACCGAACCTGGCCGTCGCG
CCAGATCACCCGCGCGCCGCGCTGGATGAGCACCGATCTGCGCGACGGCAACCAGGCGCTGATCGAACCGATGAACAGCG
CGCGCAAGCTGCGATTCTTCGAGCAACTCGTCAAGATCGGGCTCAAGGAAATCGAAGTGGCATTCCCCTCTGCATCGCAG
ACCGATTTCGATTTCGTGCGGATGCTGATCGAGGAAGATCGCATCCCCGAGGACGTCAACATCGTCGTGCTGACCCAGGC
ACGCGATGACCTGATCCGCCGCACGGTGGAGTCCGTGCGCGGCGCCAGGCGCGCGACGGTGCATCTCTACAACCCGATCG
CGCCCGCATGGCGCCGCATCGTGTTCAATGCATCGCGCGAGGAGATCAAGGAAGTGGCCGTCTCCGGCACGCGCCTGATC
CGTGAACTGACCGATGCCATGCCGGACACCGCATGGACCTACGAGTACTCGCCCGAAACCTTCAGCCTGGCCGAACTCGA
CTTCTCGCTCGAAGTATCGGACGCGGTGTCGGCCGTGTGGGAGCCCGATGCCAACCGACCGATGGTTCTGAATCTTCCGA
CCACAGTGGAATGCAGCACCACCAACGTGTTCGCGGACCAGGTCGAATGGATGCATCGCCGTCTGGCGCGGCGCGAGCAC
ATCGTGCTGTCGGTGCATCCGCACAACGATCGTGGCACTGCCGTGGCCGCGGCGGAACTGGCCGTGATGGCCGGTGCCGA
TCGCGTGGAAGGCTGTCTGTTCGGCAACGGCGAGCGCACCGGCAATGTGGATCTGGTCACGCTGGCGCTGAACCTGTACA
CACAGGGCGTGGACCCGCAACTGGACTTCTCCGATATCGACGAAGTGCGCCAGTGCGTCGAGCACTGCAATCAGTTGCCT
GTGCACCCGCGTCATCCGTACGTGGGCGATCTGGTCTTCACGGCATTCTCGGGTTCCCATCAGGACGCGATCCGCAAGGG
ATTCGCCCAGCAGAAGGACGACGCGATCTGGGAAGTGCCTTACCTGCCGATCGATCCGGCCGATCTCGGCCGCAGCTATG
ACGCGGTGATCCGTGTGAACAGCCAGTCCGGCAAGGGCGGCATGGCCTATCTGCTCGAACAGATGCACGGCATCTACATG
CCGCGCCGCCTGCAGATCGAGTTCAGTCGGGCGGTGCAAGCCATGACCGATGAAACCGGGCTCGAGGCCAGTGCCGCGGA
TCTCTACGCACTGTTCCGCCGCGAGTATCTCGACGTCGAGGCGCCACTGCGCTACGTATCGCATCGCATGGTATCGGCCA
GCGACGCCGCCGCGGAAGTGGAGATCGATATCGTGCGCGATGGTCGTCCGATGCGCATGCGTGGCACGGGCAATGGTCCG
ATCGATGCGGCAGTGGATGCGTTCACGCGCGGCCTGGGCGTGGCGGTGCGTGTGATGGACTATCACGAGCACGCCATGAC
CACCGGCGCCGATGCCAACGCGGCTTGCTATGTGGAAGTGCGCGTCGCGGGCTCGATCACGGGCTTCGGCGCGGGCGTCG
ATCCCAGTATCGTCACAGCGTCACTGAAGGCCGTGGTCTCCGGTATCAATCGCCATCTGCAAACCCGCGACATGTCGGAA
GCCGTGGAGGCCAGGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCCTGTGGCAGTTCGGCAGCCTTTCGCCACCGTTTTTCCGTCCGTTTTCCGTTTTTGACGCATTCGTCGCATCTTCCCTT
AGCGAGGAAGGAGATTTCCC

Downstream 100 bases:

>100_bases
TGACTGGCGGATGCCCTGCATCCGAGAAAACCGGTGTGCCCTCCCCCCAGAGGGCACACCGCGAAAGACGTCCTCTCGGA
CGCCCACGACATGCGCGGTG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MPMLQDPSTKYRPATTVDLHDRTWPSRQITRAPRWMSTDLRDGNQALIEPMNSARKLRFFEQLVKIGLKEIEVAFPSASQ
TDFDFVRMLIEEDRIPEDVNIVVLTQARDDLIRRTVESVRGARRATVHLYNPIAPAWRRIVFNASREEIKEVAVSGTRLI
RELTDAMPDTAWTYEYSPETFSLAELDFSLEVSDAVSAVWEPDANRPMVLNLPTTVECSTTNVFADQVEWMHRRLARREH
IVLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPQLDFSDIDEVRQCVEHCNQLP
VHPRHPYVGDLVFTAFSGSHQDAIRKGFAQQKDDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQMHGIYM
PRRLQIEFSRAVQAMTDETGLEASAADLYALFRREYLDVEAPLRYVSHRMVSASDAAAEVEIDIVRDGRPMRMRGTGNGP
IDAAVDAFTRGLGVAVRVMDYHEHAMTTGADANAACYVEVRVAGSITGFGAGVDPSIVTASLKAVVSGINRHLQTRDMSE
AVEARAA

Sequences:

>Translated_567_residues
MPMLQDPSTKYRPATTVDLHDRTWPSRQITRAPRWMSTDLRDGNQALIEPMNSARKLRFFEQLVKIGLKEIEVAFPSASQ
TDFDFVRMLIEEDRIPEDVNIVVLTQARDDLIRRTVESVRGARRATVHLYNPIAPAWRRIVFNASREEIKEVAVSGTRLI
RELTDAMPDTAWTYEYSPETFSLAELDFSLEVSDAVSAVWEPDANRPMVLNLPTTVECSTTNVFADQVEWMHRRLARREH
IVLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPQLDFSDIDEVRQCVEHCNQLP
VHPRHPYVGDLVFTAFSGSHQDAIRKGFAQQKDDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQMHGIYM
PRRLQIEFSRAVQAMTDETGLEASAADLYALFRREYLDVEAPLRYVSHRMVSASDAAAEVEIDIVRDGRPMRMRGTGNGP
IDAAVDAFTRGLGVAVRVMDYHEHAMTTGADANAACYVEVRVAGSITGFGAGVDPSIVTASLKAVVSGINRHLQTRDMSE
AVEARAA
>Mature_566_residues
PMLQDPSTKYRPATTVDLHDRTWPSRQITRAPRWMSTDLRDGNQALIEPMNSARKLRFFEQLVKIGLKEIEVAFPSASQT
DFDFVRMLIEEDRIPEDVNIVVLTQARDDLIRRTVESVRGARRATVHLYNPIAPAWRRIVFNASREEIKEVAVSGTRLIR
ELTDAMPDTAWTYEYSPETFSLAELDFSLEVSDAVSAVWEPDANRPMVLNLPTTVECSTTNVFADQVEWMHRRLARREHI
VLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPQLDFSDIDEVRQCVEHCNQLPV
HPRHPYVGDLVFTAFSGSHQDAIRKGFAQQKDDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQMHGIYMP
RRLQIEFSRAVQAMTDETGLEASAADLYALFRREYLDVEAPLRYVSHRMVSASDAAAEVEIDIVRDGRPMRMRGTGNGPI
DAAVDAFTRGLGVAVRVMDYHEHAMTTGADANAACYVEVRVAGSITGFGAGVDPSIVTASLKAVVSGINRHLQTRDMSEA
VEARAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=533, Percent_Identity=25.140712945591, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=573, Percent_Identity=48.3420593368237, Blast_Score=551, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6324682, Length=575, Percent_Identity=46.2608695652174, Blast_Score=540, Evalue=1e-154,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 63098; Mature: 62967

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMLQDPSTKYRPATTVDLHDRTWPSRQITRAPRWMSTDLRDGNQALIEPMNSARKLRFF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
EQLVKIGLKEIEVAFPSASQTDFDFVRMLIEEDRIPEDVNIVVLTQARDDLIRRTVESVR
HHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHC
GARRATVHLYNPIAPAWRRIVFNASREEIKEVAVSGTRLIRELTDAMPDTAWTYEYSPET
CCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCE
FSLAELDFSLEVSDAVSAVWEPDANRPMVLNLPTTVECSTTNVFADQVEWMHRRLARREH
EEEEEEEEEEEHHHHHHHHCCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCE
IVLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPQ
EEEEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDEVRQCVEHCNQLPVHPRHPYVGDLVFTAFSGSHQDAIRKGFAQQKDDAIWEVP
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHCCCCCCEEECC
YLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQMHGIYMPRRLQIEFSRAVQAMTDETG
CCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCC
LEASAADLYALFRREYLDVEAPLRYVSHRMVSASDAAAEVEIDIVRDGRPMRMRGTGNGP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEECCCCCC
IDAAVDAFTRGLGVAVRVMDYHEHAMTTGADANAACYVEVRVAGSITGFGAGVDPSIVTA
HHHHHHHHHHCCCEEEEEEEHHHHHHCCCCCCCEEEEEEEEEEECEEECCCCCCHHHHHH
SLKAVVSGINRHLQTRDMSEAVEARAA
HHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
PMLQDPSTKYRPATTVDLHDRTWPSRQITRAPRWMSTDLRDGNQALIEPMNSARKLRFF
CCCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
EQLVKIGLKEIEVAFPSASQTDFDFVRMLIEEDRIPEDVNIVVLTQARDDLIRRTVESVR
HHHHHCCHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHC
GARRATVHLYNPIAPAWRRIVFNASREEIKEVAVSGTRLIRELTDAMPDTAWTYEYSPET
CCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCE
FSLAELDFSLEVSDAVSAVWEPDANRPMVLNLPTTVECSTTNVFADQVEWMHRRLARREH
EEEEEEEEEEEHHHHHHHHCCCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCE
IVLSVHPHNDRGTAVAAAELAVMAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGVDPQ
EEEEEECCCCCCCCHHHHHHHHHCCCCHHCEEEECCCCCCCCEEEEEEEEEHHHCCCCCC
LDFSDIDEVRQCVEHCNQLPVHPRHPYVGDLVFTAFSGSHQDAIRKGFAQQKDDAIWEVP
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHCCCCCCEEECC
YLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLEQMHGIYMPRRLQIEFSRAVQAMTDETG
CCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCC
LEASAADLYALFRREYLDVEAPLRYVSHRMVSASDAAAEVEIDIVRDGRPMRMRGTGNGP
CCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEEECCCCCC
IDAAVDAFTRGLGVAVRVMDYHEHAMTTGADANAACYVEVRVAGSITGFGAGVDPSIVTA
HHHHHHHHHHCCCEEEEEEEHHHHHHCCCCCCCEEEEEEEEEEECEEECCCCCCHHHHHH
SLKAVVSGINRHLQTRDMSEAVEARAA
HHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA