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The map label for this gene is leuC [H]

Identifier: 94314787

GI number: 94314787

Start: 63152

End: 64474

Strand: Reverse

Name: leuC [H]

Synonym: Rmet_5868

Alternate gene names: 94314787

Gene position: 64474-63152 (Counterclockwise)

Preceding gene: 94314788

Following gene: 94314786

Centisome position: 2.5

GC content: 68.78

Gene sequence:

>1323_bases
ATGACCCTGCCGCACGGCAAGACCGCGGTCGACCCCAGCCAACTGCCCGCCACACTCGCCCAGAAACTGGTCGCTCGTGC
GGCAGGTGTGTCGCATGTCACGCCGGGATCGGTGGTGATGTGCAAGGTCGACCTGGCCATGTCGCACGACTCGAGCGGAC
CGCGCCGCGTGGCGCCGCTGCTGCGCGAACTCGGCGCAAAGGTCTGGGATCCGTCCCGCTTCGTGGTGGTCACCGACCAC
TACGTGCCTGCGGCCGACCCCGAAGCCGAATCGATTCTGCGCTTCACGCGCGACTGGGTGCGTGAAGCGGGCATTCAGAA
CTTCATCGACAACGAAGGCATCTGCCACGTGGTGCTGCCCGAGCGCGGCCATATCCTGCCCGGACGCTTCATCGTCGGCG
GCGACAGCCACAGCCCCACGGGCGGCGCTTTTGGCGCGTACATGTTCGGTATCGGCGCCACGGAAATGGCCGGTGTGCTG
GCCACGGGGGAGATCTGGCTGCGTGTGCCGAACACGATCCGGGTCGAGTGGCAGGGGACGCTGTCGGACGGCGTTTGCGC
CAAGGACATCATGCTGTTCCTGTGCGCCACGCTGGGTATGGCGGGTGGCCGCTACGAAGTGATCGAATACACCGGGAGCA
CGGTAAAGACGCTGTCGATGCAGGAGCGCATGACGCTCACGAACATGAGCACCGAACTCGGCGCGCAAACGGCGCTGATC
GCGCCGGATGCCACGACGATGGCATGGCTTGCCGATGCCGGCGTCGATGCCGCCACGCTCGCCGCGATCGAACCGCGCCA
GTGGCGCAGCGATGCCGACGCACCGGTACTGGCCACACATCGCTTCGATGCCGGCACGCTGGTACCGCAGGTAGCCGCGC
CGCATTCGCCGGCCAACAGCGGCCCGGTCGACCAGGCCGCCGGCGCGCCGATCGAGATCGCCTATCTGGGCGCCTGCACC
GGCGCCAAGCTCGAAGACCTGCGCATGGCCGCGCGCGTGCTGCGCGGGCGCAAGGTGGCACCGGGCGTGTCGTTGAAGGT
CGCGCCGGCCTCGCTGCGCGACCAGCAACAAGCGGCCACCGAAGGGACGCTCGGCGTGCTGATGGATGCCGGCGCGGAGC
TGCTGCCGAACGCGTGCAATGCGTGCGCCGGCTACGGCGCCACACGCTTTCCGGCCGGCAGCCGCGCCATCGCCTCGACC
GCGCGCAACTTCCCCGGCCGCATGGGCGATGCAGGTAGCGCCGTATGGCTGGCATCGCCCATGACCGTGGCTGCCAGCGC
CGCCACCGGCTGCATCACTGACCCACGCACGTTACTGGCATGA

Upstream 100 bases:

>100_bases
CGCAGCCCAGTGCCATGCCGCCGGAGGCGTTCGCGAATTTCGTGAAGCAGGACGTCAAGCGCTGGGCCACGGTGGTGAAG
TATTCAGGAGCCAAGCCCGA

Downstream 100 bases:

>100_bases
CCCCGGCTCCGGATTTCCACCCCCCGCCCTCTGGCCGCATCTGGCGCTTCGGCGAAAACGTCGATACCGACGCGATGGCG
CCGGGCCGATTCATGAAGGA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MTLPHGKTAVDPSQLPATLAQKLVARAAGVSHVTPGSVVMCKVDLAMSHDSSGPRRVAPLLRELGAKVWDPSRFVVVTDH
YVPAADPEAESILRFTRDWVREAGIQNFIDNEGICHVVLPERGHILPGRFIVGGDSHSPTGGAFGAYMFGIGATEMAGVL
ATGEIWLRVPNTIRVEWQGTLSDGVCAKDIMLFLCATLGMAGGRYEVIEYTGSTVKTLSMQERMTLTNMSTELGAQTALI
APDATTMAWLADAGVDAATLAAIEPRQWRSDADAPVLATHRFDAGTLVPQVAAPHSPANSGPVDQAAGAPIEIAYLGACT
GAKLEDLRMAARVLRGRKVAPGVSLKVAPASLRDQQQAATEGTLGVLMDAGAELLPNACNACAGYGATRFPAGSRAIAST
ARNFPGRMGDAGSAVWLASPMTVAASAATGCITDPRTLLA

Sequences:

>Translated_440_residues
MTLPHGKTAVDPSQLPATLAQKLVARAAGVSHVTPGSVVMCKVDLAMSHDSSGPRRVAPLLRELGAKVWDPSRFVVVTDH
YVPAADPEAESILRFTRDWVREAGIQNFIDNEGICHVVLPERGHILPGRFIVGGDSHSPTGGAFGAYMFGIGATEMAGVL
ATGEIWLRVPNTIRVEWQGTLSDGVCAKDIMLFLCATLGMAGGRYEVIEYTGSTVKTLSMQERMTLTNMSTELGAQTALI
APDATTMAWLADAGVDAATLAAIEPRQWRSDADAPVLATHRFDAGTLVPQVAAPHSPANSGPVDQAAGAPIEIAYLGACT
GAKLEDLRMAARVLRGRKVAPGVSLKVAPASLRDQQQAATEGTLGVLMDAGAELLPNACNACAGYGATRFPAGSRAIAST
ARNFPGRMGDAGSAVWLASPMTVAASAATGCITDPRTLLA
>Mature_439_residues
TLPHGKTAVDPSQLPATLAQKLVARAAGVSHVTPGSVVMCKVDLAMSHDSSGPRRVAPLLRELGAKVWDPSRFVVVTDHY
VPAADPEAESILRFTRDWVREAGIQNFIDNEGICHVVLPERGHILPGRFIVGGDSHSPTGGAFGAYMFGIGATEMAGVLA
TGEIWLRVPNTIRVEWQGTLSDGVCAKDIMLFLCATLGMAGGRYEVIEYTGSTVKTLSMQERMTLTNMSTELGAQTALIA
PDATTMAWLADAGVDAATLAAIEPRQWRSDADAPVLATHRFDAGTLVPQVAAPHSPANSGPVDQAAGAPIEIAYLGACTG
AKLEDLRMAARVLRGRKVAPGVSLKVAPASLRDQQQAATEGTLGVLMDAGAELLPNACNACAGYGATRFPAGSRAIASTA
RNFPGRMGDAGSAVWLASPMTVAASAATGCITDPRTLLA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=344, Percent_Identity=28.7790697674419, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI41352693, Length=394, Percent_Identity=23.6040609137056, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI8659555, Length=394, Percent_Identity=22.8426395939086, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=32.5531914893617, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI2367097, Length=428, Percent_Identity=23.8317757009346, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787531, Length=388, Percent_Identity=23.9690721649485, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=339, Percent_Identity=27.4336283185841, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI32564738, Length=339, Percent_Identity=27.4336283185841, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17568399, Length=382, Percent_Identity=24.0837696335079, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25149342, Length=285, Percent_Identity=26.3157894736842, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=430, Percent_Identity=33.4883720930233, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=480, Percent_Identity=28.9583333333333, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6323335, Length=337, Percent_Identity=29.080118694362, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=371, Percent_Identity=26.6846361185984, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571643, Length=335, Percent_Identity=28.0597014925373, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161076999, Length=336, Percent_Identity=27.0833333333333, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281365315, Length=336, Percent_Identity=27.0833333333333, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17864292, Length=336, Percent_Identity=27.0833333333333, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 45860; Mature: 45729

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPHGKTAVDPSQLPATLAQKLVARAAGVSHVTPGSVVMCKVDLAMSHDSSGPRRVAPL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHH
LRELGAKVWDPSRFVVVTDHYVPAADPEAESILRFTRDWVREAGIQNFIDNEGICHVVLP
HHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
ERGHILPGRFIVGGDSHSPTGGAFGAYMFGIGATEMAGVLATGEIWLRVPNTIRVEWQGT
CCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHCEEEECEEEEECCCEEEEEEECC
LSDGVCAKDIMLFLCATLGMAGGRYEVIEYTGSTVKTLSMQERMTLTNMSTELGAQTALI
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCHHHCCCEEEE
APDATTMAWLADAGVDAATLAAIEPRQWRSDADAPVLATHRFDAGTLVPQVAAPHSPANS
CCCCHHHHHHHHCCCCHHHEEECCCHHHCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCC
GPVDQAAGAPIEIAYLGACTGAKLEDLRMAARVLRGRKVAPGVSLKVAPASLRDQQQAAT
CCCCHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHH
EGTLGVLMDAGAELLPNACNACAGYGATRFPAGSRAIASTARNFPGRMGDAGSAVWLASP
CCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECC
MTVAASAATGCITDPRTLLA
HHHHHHCCCCCCCCCHHHCC
>Mature Secondary Structure 
TLPHGKTAVDPSQLPATLAQKLVARAAGVSHVTPGSVVMCKVDLAMSHDSSGPRRVAPL
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHH
LRELGAKVWDPSRFVVVTDHYVPAADPEAESILRFTRDWVREAGIQNFIDNEGICHVVLP
HHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
ERGHILPGRFIVGGDSHSPTGGAFGAYMFGIGATEMAGVLATGEIWLRVPNTIRVEWQGT
CCCCCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHCEEEECEEEEECCCEEEEEEECC
LSDGVCAKDIMLFLCATLGMAGGRYEVIEYTGSTVKTLSMQERMTLTNMSTELGAQTALI
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEHHHHHHHHHHHCCHHHCCCEEEE
APDATTMAWLADAGVDAATLAAIEPRQWRSDADAPVLATHRFDAGTLVPQVAAPHSPANS
CCCCHHHHHHHHCCCCHHHEEECCCHHHCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCC
GPVDQAAGAPIEIAYLGACTGAKLEDLRMAARVLRGRKVAPGVSLKVAPASLRDQQQAAT
CCCCHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHH
EGTLGVLMDAGAELLPNACNACAGYGATRFPAGSRAIASTARNFPGRMGDAGSAVWLASP
CCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECC
MTVAASAATGCITDPRTLLA
HHHHHHCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA