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The map label for this gene is epsB1 [H]

Identifier: 94314763

GI number: 94314763

Start: 34430

End: 36772

Strand: Direct

Name: epsB1 [H]

Synonym: Rmet_5844

Alternate gene names: 94314763

Gene position: 34430-36772 (Clockwise)

Preceding gene: 94314762

Following gene: 94314764

Centisome position: 1.33

GC content: 67.73

Gene sequence:

>2343_bases
GTGAGCACCATGTCATCTGCCGGCGCGATGCCGGCGCCAGGGAGCAGCGCCACGCGCAAGGGGGGGCGCCATTTGCGCGC
ATGGATGGACCATGCTGGCTGGATTGTCGCGGCTGGCGTCATTGGTGCCACGATTGCCGCGATGATCGGCATTTCCCGTC
CCAATCTCTACGATGCCCACACGCTCGTGCAGATCTATCAGAAGGGTGACGGCTCGGGCACCACGCAGCGCACCGAACCG
TTCGACGTGGGGATGCTGCGCAGCCGCGCCGTGGTCGGCCCGGTGGTGGAGCGCCTGCGCCTGTACATCTCCGTCGAGCC
GCTGCGCGCGCCATTGCTGGGCGGCGTGGCCGCGCACTTCGCGCAGGCGGGCAAGCTCCGGGGACCGTGGCCGGAGCGGC
TCGGTTACGCGTGGGGCGGCGAGCGGGTCGACATCCGCACCCTGGACGTGCCGGAGCGCCTGCTCAACGTGCCAATGACG
CTCGAAGTGTTGCCTGGGGATGCCTATCGCCTGACGCTCGACGGCGAGCTCGTGACCGATGGCAAGATCGGCAGCCGTGT
CGAAGAGAATGGCGTGTCCATCCTGGTGTCGCGCATCGACGCGCGCGCGGGCACGCGATTCACTGTCACACGGCACGACA
CGGCGCAGACGATCGATACCGTGGCGCGTGAACTGCGCATCGGCAGCGATGGCAGCGATACCAGCACGGTACGCATCGCG
TGGCAGAGCAGTGACCGCACCGCGGTAGCCGCGCTGATCAACGGCATCGCCGATTCCTACATTACCGGACAGGCCGAGAC
GCGCCGCGACGAGGCAGCCTCCACGCTGGCCTTCCTGAGCGGCGAACTGCCGCGTGTCAAGGACGAGCTCGAACGCGCCG
AGGCGGCGCTCACGCGCTACCGCTCGCGCACGGGATCGATGCAGCCGAGCCAGGACGCCCAGTCGTACCTGAACAGCAGC
ATCGATTACCAGAAGCAGATCGCCCAGCTCAAGCTCGACCGCACCAAGCTGCTGCAGCGCTTTACGGAAGAGTCGAACGA
AGTACGAACGATGGACAGCCAGATCCAGCAGCTCACGCGGGACCGCAAGGAGCTGGATACCCGGATGCAGACGCTGTCGG
TCTCCGAGCGCGAGTCCGTGGCCCTGACGCGTGACGTCAAGGTGGCCGAGGACATGTACATGTCCTTGCGCAACCGGCTC
GAACAGCTCTCGCTGGCCAAGCTCGACAGCACCCGGCAGACCCGCGTGGTCGACATCGCGCTGACACCGGTCTCGCCAAT
CGGCGTGGGCACCGCGCCGCTCACGATGGGCGGCGGGTTGCTGGGCATCTGCCTGGCGATGTGCGTGGTCTCGGTGCGCC
AGCGCATGAAGCCGGCCGTGGCCACCGCCAATGATGCCGAGGAGACGCTTGGCCTGGCCATGCTCGGGGATGTCGCGTAC
AGCCGCGAACAGGTGGAACTGGAGCGGCTGCTGACGGCGAAGGACCAGGATATCGGGACAATGCTGGCGCTGCAGGCGCC
GGAGCCCGGGCGCCAGGTCGCGCAGGCGTTGCCGCATGGGGCCGGCGATGCCGAGCCGGGTCATTGGGGTTTGCATGATC
AATACCTGCTGGCCCGCAACGCGCCGCATTCGATGGCCGTGGAGGGCCTGCGCAGCGTGCGCGCGGCACTGCATTTCCTG
CGTCGCGAGGGCAGCAACAAGGTGGTGGCCGTGACCAGCCCCACCGCAGGGGCGGGCAAGACGTTTGTCTCGGTCAACCT
GGCTGTGCTGTTCGCCGAAGCGGGCCAGCGCGTGTTGCTCGTCGATGCCGACCTGCGCCGAGGGCGGGTGTCGAGCTGGT
TCGGCCAGCCGGCGGGTCCGGGTATGGCCGAAGTGCTGGCCGGCCGCGCCACGCTGGTCGACGCGGCACGGCCAACCGTC
GTCAATGGGCTGTCGATCCTGCCTGCCGGCGCGCCGCCTACGAACCCGTCGGAACTGCTTATGCGTCCTGCCTTTGCGGA
TTGCCTGCGTGCTGGTGCGGAGCGCTTCGACCTCGTCCTGATCGATACCCCGCCCGTTCTGGCGGTGGCGGATGCCATGC
TCGTCGCCAATGTCGTGGGCGCGACGTTGCTCGTGCTGCGGGCCGATTCGACGCTGCCGTCGCAGGTCGAGGAAACCATC
AAGCGCCTGACCCGCGCCGGGGCCAGTCTCTCGGGCGGCATTCTCAACGGCGTGATGCCGAAGCGCAGCAACCGCGTCGG
CTTCGGCACCATGAATCCCTATCTCGGCATGCCCACGGTCATGCCAGCGGTCCGGCAGATTGCCCACGTCCCTGCCGTGG
AAGACAAGACAACAACGAGCTGA

Upstream 100 bases:

>100_bases
GACATCGTCAGAGTCGAGGACAACTACGGACGCAAGTAGTCCCGCAGCCTCCGGTGCGCCATGGCCACCGGGAGCCAGCC
AGGCGTCAGGGGAGTATTAC

Downstream 100 bases:

>100_bases
CGGGCCTGGCCCGGCGGGGTGCGTACCCGTTTCTGCGGACGCCGGTCTGGGCGACATCGCGGAGGCGGGCTGGCGTGGGC
ACCCCGTCCATTGCAACGGG

Product: protein-tyrosine kinase

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: EPS I polysaccharide export protein epsB [H]

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MSTMSSAGAMPAPGSSATRKGGRHLRAWMDHAGWIVAAGVIGATIAAMIGISRPNLYDAHTLVQIYQKGDGSGTTQRTEP
FDVGMLRSRAVVGPVVERLRLYISVEPLRAPLLGGVAAHFAQAGKLRGPWPERLGYAWGGERVDIRTLDVPERLLNVPMT
LEVLPGDAYRLTLDGELVTDGKIGSRVEENGVSILVSRIDARAGTRFTVTRHDTAQTIDTVARELRIGSDGSDTSTVRIA
WQSSDRTAVAALINGIADSYITGQAETRRDEAASTLAFLSGELPRVKDELERAEAALTRYRSRTGSMQPSQDAQSYLNSS
IDYQKQIAQLKLDRTKLLQRFTEESNEVRTMDSQIQQLTRDRKELDTRMQTLSVSERESVALTRDVKVAEDMYMSLRNRL
EQLSLAKLDSTRQTRVVDIALTPVSPIGVGTAPLTMGGGLLGICLAMCVVSVRQRMKPAVATANDAEETLGLAMLGDVAY
SREQVELERLLTAKDQDIGTMLALQAPEPGRQVAQALPHGAGDAEPGHWGLHDQYLLARNAPHSMAVEGLRSVRAALHFL
RREGSNKVVAVTSPTAGAGKTFVSVNLAVLFAEAGQRVLLVDADLRRGRVSSWFGQPAGPGMAEVLAGRATLVDAARPTV
VNGLSILPAGAPPTNPSELLMRPAFADCLRAGAERFDLVLIDTPPVLAVADAMLVANVVGATLLVLRADSTLPSQVEETI
KRLTRAGASLSGGILNGVMPKRSNRVGFGTMNPYLGMPTVMPAVRQIAHVPAVEDKTTTS

Sequences:

>Translated_780_residues
MSTMSSAGAMPAPGSSATRKGGRHLRAWMDHAGWIVAAGVIGATIAAMIGISRPNLYDAHTLVQIYQKGDGSGTTQRTEP
FDVGMLRSRAVVGPVVERLRLYISVEPLRAPLLGGVAAHFAQAGKLRGPWPERLGYAWGGERVDIRTLDVPERLLNVPMT
LEVLPGDAYRLTLDGELVTDGKIGSRVEENGVSILVSRIDARAGTRFTVTRHDTAQTIDTVARELRIGSDGSDTSTVRIA
WQSSDRTAVAALINGIADSYITGQAETRRDEAASTLAFLSGELPRVKDELERAEAALTRYRSRTGSMQPSQDAQSYLNSS
IDYQKQIAQLKLDRTKLLQRFTEESNEVRTMDSQIQQLTRDRKELDTRMQTLSVSERESVALTRDVKVAEDMYMSLRNRL
EQLSLAKLDSTRQTRVVDIALTPVSPIGVGTAPLTMGGGLLGICLAMCVVSVRQRMKPAVATANDAEETLGLAMLGDVAY
SREQVELERLLTAKDQDIGTMLALQAPEPGRQVAQALPHGAGDAEPGHWGLHDQYLLARNAPHSMAVEGLRSVRAALHFL
RREGSNKVVAVTSPTAGAGKTFVSVNLAVLFAEAGQRVLLVDADLRRGRVSSWFGQPAGPGMAEVLAGRATLVDAARPTV
VNGLSILPAGAPPTNPSELLMRPAFADCLRAGAERFDLVLIDTPPVLAVADAMLVANVVGATLLVLRADSTLPSQVEETI
KRLTRAGASLSGGILNGVMPKRSNRVGFGTMNPYLGMPTVMPAVRQIAHVPAVEDKTTTS
>Mature_779_residues
STMSSAGAMPAPGSSATRKGGRHLRAWMDHAGWIVAAGVIGATIAAMIGISRPNLYDAHTLVQIYQKGDGSGTTQRTEPF
DVGMLRSRAVVGPVVERLRLYISVEPLRAPLLGGVAAHFAQAGKLRGPWPERLGYAWGGERVDIRTLDVPERLLNVPMTL
EVLPGDAYRLTLDGELVTDGKIGSRVEENGVSILVSRIDARAGTRFTVTRHDTAQTIDTVARELRIGSDGSDTSTVRIAW
QSSDRTAVAALINGIADSYITGQAETRRDEAASTLAFLSGELPRVKDELERAEAALTRYRSRTGSMQPSQDAQSYLNSSI
DYQKQIAQLKLDRTKLLQRFTEESNEVRTMDSQIQQLTRDRKELDTRMQTLSVSERESVALTRDVKVAEDMYMSLRNRLE
QLSLAKLDSTRQTRVVDIALTPVSPIGVGTAPLTMGGGLLGICLAMCVVSVRQRMKPAVATANDAEETLGLAMLGDVAYS
REQVELERLLTAKDQDIGTMLALQAPEPGRQVAQALPHGAGDAEPGHWGLHDQYLLARNAPHSMAVEGLRSVRAALHFLR
REGSNKVVAVTSPTAGAGKTFVSVNLAVLFAEAGQRVLLVDADLRRGRVSSWFGQPAGPGMAEVLAGRATLVDAARPTVV
NGLSILPAGAPPTNPSELLMRPAFADCLRAGAERFDLVLIDTPPVLAVADAMLVANVVGATLLVLRADSTLPSQVEETIK
RLTRAGASLSGGILNGVMPKRSNRVGFGTMNPYLGMPTVMPAVRQIAHVPAVEDKTTTS

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family [H]

Homologues:

Organism=Escherichia coli, GI87082032, Length=758, Percent_Identity=28.1002638522427, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI1787216, Length=727, Percent_Identity=27.5103163686382, Blast_Score=285, Evalue=7e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR005700
- InterPro:   IPR003856 [H]

Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 83725; Mature: 83594

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00027 HOMEOBOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMSSAGAMPAPGSSATRKGGRHLRAWMDHAGWIVAAGVIGATIAAMIGISRPNLYDAH
CCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
TLVQIYQKGDGSGTTQRTEPFDVGMLRSRAVVGPVVERLRLYISVEPLRAPLLGGVAAHF
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHH
AQAGKLRGPWPERLGYAWGGERVDIRTLDVPERLLNVPMTLEVLPGDAYRLTLDGELVTD
HHCCCCCCCCHHHCCCCCCCCEEEEEECCCCHHHHCCCEEEEEECCCEEEEEECCEEEEC
GKIGSRVEENGVSILVSRIDARAGTRFTVTRHDTAQTIDTVARELRIGSDGSDTSTVRIA
CCCCCCHHHCCCEEEEEHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
WQSSDRTAVAALINGIADSYITGQAETRRDEAASTLAFLSGELPRVKDELERAEAALTRY
EECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RSRTGSMQPSQDAQSYLNSSIDYQKQIAQLKLDRTKLLQRFTEESNEVRTMDSQIQQLTR
HHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DRKELDTRMQTLSVSERESVALTRDVKVAEDMYMSLRNRLEQLSLAKLDSTRQTRVVDIA
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
LTPVSPIGVGTAPLTMGGGLLGICLAMCVVSVRQRMKPAVATANDAEETLGLAMLGDVAY
ECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHH
SREQVELERLLTAKDQDIGTMLALQAPEPGRQVAQALPHGAGDAEPGHWGLHDQYLLARN
HHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECC
APHSMAVEGLRSVRAALHFLRREGSNKVVAVTSPTAGAGKTFVSVNLAVLFAEAGQRVLL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHEEEEECCCCEEEE
VDADLRRGRVSSWFGQPAGPGMAEVLAGRATLVDAARPTVVNGLSILPAGAPPTNPSELL
EECCCCCCCHHHHCCCCCCCCHHHHHCCCHHHHHCCCCHHHCCCEEEECCCCCCCHHHHH
MRPAFADCLRAGAERFDLVLIDTPPVLAVADAMLVANVVGATLLVLRADSTLPSQVEETI
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
KRLTRAGASLSGGILNGVMPKRSNRVGFGTMNPYLGMPTVMPAVRQIAHVPAVEDKTTTS
HHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
STMSSAGAMPAPGSSATRKGGRHLRAWMDHAGWIVAAGVIGATIAAMIGISRPNLYDAH
CCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHH
TLVQIYQKGDGSGTTQRTEPFDVGMLRSRAVVGPVVERLRLYISVEPLRAPLLGGVAAHF
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHH
AQAGKLRGPWPERLGYAWGGERVDIRTLDVPERLLNVPMTLEVLPGDAYRLTLDGELVTD
HHCCCCCCCCHHHCCCCCCCCEEEEEECCCCHHHHCCCEEEEEECCCEEEEEECCEEEEC
GKIGSRVEENGVSILVSRIDARAGTRFTVTRHDTAQTIDTVARELRIGSDGSDTSTVRIA
CCCCCCHHHCCCEEEEEHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
WQSSDRTAVAALINGIADSYITGQAETRRDEAASTLAFLSGELPRVKDELERAEAALTRY
EECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RSRTGSMQPSQDAQSYLNSSIDYQKQIAQLKLDRTKLLQRFTEESNEVRTMDSQIQQLTR
HHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DRKELDTRMQTLSVSERESVALTRDVKVAEDMYMSLRNRLEQLSLAKLDSTRQTRVVDIA
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
LTPVSPIGVGTAPLTMGGGLLGICLAMCVVSVRQRMKPAVATANDAEETLGLAMLGDVAY
ECCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHH
SREQVELERLLTAKDQDIGTMLALQAPEPGRQVAQALPHGAGDAEPGHWGLHDQYLLARN
HHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECC
APHSMAVEGLRSVRAALHFLRREGSNKVVAVTSPTAGAGKTFVSVNLAVLFAEAGQRVLL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEHEEEEECCCCEEEE
VDADLRRGRVSSWFGQPAGPGMAEVLAGRATLVDAARPTVVNGLSILPAGAPPTNPSELL
EECCCCCCCHHHHCCCCCCCCHHHHHCCCHHHHHCCCCHHHCCCEEEECCCCCCCHHHHH
MRPAFADCLRAGAERFDLVLIDTPPVLAVADAMLVANVVGATLLVLRADSTLPSQVEETI
HHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
KRLTRAGASLSGGILNGVMPKRSNRVGFGTMNPYLGMPTVMPAVRQIAHVPAVEDKTTTS
HHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]