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The map label for this gene is ytcI [H]

Identifier: 94314662

GI number: 94314662

Start: 2504148

End: 2505851

Strand: Reverse

Name: ytcI [H]

Synonym: Rmet_5743

Alternate gene names: 94314662

Gene position: 2505851-2504148 (Counterclockwise)

Preceding gene: 94314667

Following gene: 94314661

Centisome position: 97.12

GC content: 62.03

Gene sequence:

>1704_bases
ATGAAAGACGGGGCATCAGGAGCCGCAGCATTCGTCGCCGCACGGGACTTTCTGCTGCGACATCGCGCGGACTACGATCG
CGCATATGGGGAATTTGCCTGGCCACAGCTCGGCGAGTTCAACTGGGCGCTCGATTACTTCGACGTCATGGCGCAGGGAA
ACGACAATCCCGCACTCTGGATTGTGGACAGCCCCAATGATGCAGGCGCGCCATTGTCTTTCAGGGAGATGTCGGAGCGC
TCTTCTCGCATGGCCAACTACTTACGCGGCTTGGGTGTCTCGCGCGGCGACCGGCTCCTCCTGATGCTGCCCAATCGGGT
CGAGTTGTGGGATGTCATGCTGGCGGCGATGAAGCTCGGTGCCGTTGTGCTGCCTGCCACGACCCAGTTATCTCCCGACG
ATGTTCGCGATCGCGTGCAACTCGGCGAGGCGCGGTTCGTCGTGGTCGACGGTGCGGAGCTGGGAAAATTCGAAGATGTT
GACGCGAGCGTCACCCGTATCGCGGTGGGCGAGCGCGTCGCAGGGTGGCATCACATTGGTGACGCGTATCAGGCGTCGCC
AACCTTCGTCCCGGACGGGGTAACCCGCGCGACGGATCTGCTGCTCCTGTATTTCACGTCGGGCACGACATCGAAACCAA
AGCTGGTCGAACACACGCATCAGAGCTATCCCGTTGGCCACTTGTCGACGATGTACTGGATTGGGCTCCAGCCCGGGGAC
ATTCACTGGAACATCAGTTCCCCGGGATGGGCCAAGCACGCATGGAGTTGCTTCTACGCGCCGTGGAATGCCCAGGCGTG
CGTCTTCATCTACAACTTCGCCCGCTTCGCTCCGAGGGATGCGCTGGACGTGCTCGTGCAACGCAACGTCACTACGCTCT
GCGCCCCGCCAACGGTATGGCGAATGCTCGTGCAGGAGCCGTTGGCTTCGTATGCGGTAAAGCTGCGCGAGATCGTCGGG
GCAGGAGAACCGCTCAACCCGGAAATCATCGAGCGGGTGCAGAGCGCGTGGGGCATCACCATTCGCGACGGATTCGGGCA
GACCGAGACAACCTGCCAAATCGGCAATCCGCCGGGTCAGCCCGTCGTGCCAGGCTCGATGGGCAGGCCGCTTCCCGGGT
ACCGTGTCGATCTGGTGGATCTGGATGATCATCCCGCAAACGAGGGCGAGATTGTCCTCTCGCTGGCCAGCCGCCCGCTT
GGATTGATGGCGGGCTACTCGAACAACGAGAAGGCGACTGCGGAAGCCATGCGCAATGGCTTCTATCACACGTCCGATAT
CGCGATGCGCCGGGACGACGGGTACCTTGTCTACGTGGGTCGCGCGGACGATGTCTTCAAGGCATCGGACTACCGGCTGA
GTCCATTCGAGCTGGAGAGCGTCCTGATCGAACACGAGGCCATTGGCGAGGCCGCCGTTGTGCCGAGCCCGGACCCGGTA
AAGCTGTATGTGCCGAAGGCCTATGTCACGGTTCGCCAGGGCTACGAGGCTGGCCCCGAACTGGCCAGGGCGGTATTCCG
CTTTTCGAGAGAGAAGCTCGCTCCGTACAAGCGGATTCGCAGGCTGCAGTTCAGCGAGCTACCCAAGACGATCTCCGGAA
AGATCCGGCGCGTTGACCTCCGCCGCCGCGAGATGGAGCGCGAGGATCAGTCGTCGAGGATGCCCGGTGAATACTGGGAG
GAAGATTTCCCCGACTTGCGTTGA

Upstream 100 bases:

>100_bases
GGTACGGTTGGGTGTTGGCTCGAATGCCGCGCACCGTTCAGGGTATTGGTAATTCGCTGTTCATTCGAGAGCGCTCTACC
GCGCCAACCAGGAGACAAGG

Downstream 100 bases:

>100_bases
CTGATGTTGACTGATGGCGTGGCAAGACCCGCGGAGGGCGGATCACGTGCTGCAAGTGCTCGAAGGCATTGGGAAGCTGT
CGCTCGATAATGGGTGGGAT

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MKDGASGAAAFVAARDFLLRHRADYDRAYGEFAWPQLGEFNWALDYFDVMAQGNDNPALWIVDSPNDAGAPLSFREMSER
SSRMANYLRGLGVSRGDRLLLMLPNRVELWDVMLAAMKLGAVVLPATTQLSPDDVRDRVQLGEARFVVVDGAELGKFEDV
DASVTRIAVGERVAGWHHIGDAYQASPTFVPDGVTRATDLLLLYFTSGTTSKPKLVEHTHQSYPVGHLSTMYWIGLQPGD
IHWNISSPGWAKHAWSCFYAPWNAQACVFIYNFARFAPRDALDVLVQRNVTTLCAPPTVWRMLVQEPLASYAVKLREIVG
AGEPLNPEIIERVQSAWGITIRDGFGQTETTCQIGNPPGQPVVPGSMGRPLPGYRVDLVDLDDHPANEGEIVLSLASRPL
GLMAGYSNNEKATAEAMRNGFYHTSDIAMRRDDGYLVYVGRADDVFKASDYRLSPFELESVLIEHEAIGEAAVVPSPDPV
KLYVPKAYVTVRQGYEAGPELARAVFRFSREKLAPYKRIRRLQFSELPKTISGKIRRVDLRRREMEREDQSSRMPGEYWE
EDFPDLR

Sequences:

>Translated_567_residues
MKDGASGAAAFVAARDFLLRHRADYDRAYGEFAWPQLGEFNWALDYFDVMAQGNDNPALWIVDSPNDAGAPLSFREMSER
SSRMANYLRGLGVSRGDRLLLMLPNRVELWDVMLAAMKLGAVVLPATTQLSPDDVRDRVQLGEARFVVVDGAELGKFEDV
DASVTRIAVGERVAGWHHIGDAYQASPTFVPDGVTRATDLLLLYFTSGTTSKPKLVEHTHQSYPVGHLSTMYWIGLQPGD
IHWNISSPGWAKHAWSCFYAPWNAQACVFIYNFARFAPRDALDVLVQRNVTTLCAPPTVWRMLVQEPLASYAVKLREIVG
AGEPLNPEIIERVQSAWGITIRDGFGQTETTCQIGNPPGQPVVPGSMGRPLPGYRVDLVDLDDHPANEGEIVLSLASRPL
GLMAGYSNNEKATAEAMRNGFYHTSDIAMRRDDGYLVYVGRADDVFKASDYRLSPFELESVLIEHEAIGEAAVVPSPDPV
KLYVPKAYVTVRQGYEAGPELARAVFRFSREKLAPYKRIRRLQFSELPKTISGKIRRVDLRRREMEREDQSSRMPGEYWE
EDFPDLR
>Mature_567_residues
MKDGASGAAAFVAARDFLLRHRADYDRAYGEFAWPQLGEFNWALDYFDVMAQGNDNPALWIVDSPNDAGAPLSFREMSER
SSRMANYLRGLGVSRGDRLLLMLPNRVELWDVMLAAMKLGAVVLPATTQLSPDDVRDRVQLGEARFVVVDGAELGKFEDV
DASVTRIAVGERVAGWHHIGDAYQASPTFVPDGVTRATDLLLLYFTSGTTSKPKLVEHTHQSYPVGHLSTMYWIGLQPGD
IHWNISSPGWAKHAWSCFYAPWNAQACVFIYNFARFAPRDALDVLVQRNVTTLCAPPTVWRMLVQEPLASYAVKLREIVG
AGEPLNPEIIERVQSAWGITIRDGFGQTETTCQIGNPPGQPVVPGSMGRPLPGYRVDLVDLDDHPANEGEIVLSLASRPL
GLMAGYSNNEKATAEAMRNGFYHTSDIAMRRDDGYLVYVGRADDVFKASDYRLSPFELESVLIEHEAIGEAAVVPSPDPV
KLYVPKAYVTVRQGYEAGPELARAVFRFSREKLAPYKRIRRLQFSELPKTISGKIRRVDLRRREMEREDQSSRMPGEYWE
EDFPDLR

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=528, Percent_Identity=36.7424242424242, Blast_Score=354, Evalue=2e-97,
Organism=Homo sapiens, GI38505220, Length=528, Percent_Identity=36.9318181818182, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI122937307, Length=526, Percent_Identity=38.212927756654, Blast_Score=327, Evalue=3e-89,
Organism=Homo sapiens, GI58082049, Length=558, Percent_Identity=34.0501792114695, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI157311624, Length=558, Percent_Identity=33.6917562724014, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI157311622, Length=558, Percent_Identity=33.6917562724014, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI115511026, Length=549, Percent_Identity=30.4189435336976, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI42544134, Length=356, Percent_Identity=34.2696629213483, Blast_Score=225, Evalue=7e-59,
Organism=Homo sapiens, GI195947378, Length=434, Percent_Identity=29.7235023041475, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI8923896, Length=602, Percent_Identity=26.2458471760797, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI13375727, Length=559, Percent_Identity=27.1914132379249, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI253970489, Length=370, Percent_Identity=32.1621621621622, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI28416953, Length=562, Percent_Identity=25.8007117437722, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI31982927, Length=533, Percent_Identity=21.2007504690432, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI187761345, Length=385, Percent_Identity=23.6363636363636, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI187761343, Length=385, Percent_Identity=23.6363636363636, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=23.8721804511278, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1790505, Length=562, Percent_Identity=30.4270462633452, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1788107, Length=545, Percent_Identity=25.5045871559633, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI221142682, Length=502, Percent_Identity=24.7011952191235, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1786529, Length=533, Percent_Identity=25.515947467167, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI145693145, Length=508, Percent_Identity=24.8031496062992, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786810, Length=523, Percent_Identity=21.9885277246654, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788595, Length=409, Percent_Identity=24.2053789731051, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=545, Percent_Identity=28.8073394495413, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71983001, Length=545, Percent_Identity=28.8073394495413, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=531, Percent_Identity=24.2937853107345, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=545, Percent_Identity=24.7706422018349, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=496, Percent_Identity=23.5887096774194, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=368, Percent_Identity=26.6304347826087, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=368, Percent_Identity=26.6304347826087, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994703, Length=368, Percent_Identity=26.6304347826087, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=27.6536312849162, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=23.3208955223881, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=22.289156626506, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=534, Percent_Identity=22.0973782771536, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32563687, Length=496, Percent_Identity=22.5806451612903, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=23.7827715355805, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115534440, Length=470, Percent_Identity=21.063829787234, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=364, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=576, Percent_Identity=27.4305555555556, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319264, Length=578, Percent_Identity=25.6055363321799, Blast_Score=133, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=26.4788732394366, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24667955, Length=588, Percent_Identity=28.4013605442177, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=29.7196261682243, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24667959, Length=495, Percent_Identity=29.4949494949495, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355181, Length=513, Percent_Identity=26.3157894736842, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21355225, Length=574, Percent_Identity=26.4808362369338, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=24.0480961923848, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24581924, Length=364, Percent_Identity=26.0989010989011, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648257, Length=499, Percent_Identity=24.8496993987976, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 63418; Mature: 63418

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDGASGAAAFVAARDFLLRHRADYDRAYGEFAWPQLGEFNWALDYFDVMAQGNDNPALW
CCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEE
IVDSPNDAGAPLSFREMSERSSRMANYLRGLGVSRGDRLLLMLPNRVELWDVMLAAMKLG
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHC
AVVLPATTQLSPDDVRDRVQLGEARFVVVDGAELGKFEDVDASVTRIAVGERVAGWHHIG
EEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHEEECCCHHCCHHHCC
DAYQASPTFVPDGVTRATDLLLLYFTSGTTSKPKLVEHTHQSYPVGHLSTMYWIGLQPGD
CCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCC
IHWNISSPGWAKHAWSCFYAPWNAQACVFIYNFARFAPRDALDVLVQRNVTTLCAPPTVW
EEEECCCCCCCCCCCEEEECCCCCCEEEEEEHHHHHCCHHHHHHHHHCCCCEEECCHHHH
RMLVQEPLASYAVKLREIVGAGEPLNPEIIERVQSAWGITIRDGFGQTETTCQIGNPPGQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCEEECCCCCCC
PVVPGSMGRPLPGYRVDLVDLDDHPANEGEIVLSLASRPLGLMAGYSNNEKATAEAMRNG
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHCC
FYHTSDIAMRRDDGYLVYVGRADDVFKASDYRLSPFELESVLIEHEAIGEAAVVPSPDPV
CEECCCEEEEECCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCE
KLYVPKAYVTVRQGYEAGPELARAVFRFSREKLAPYKRIRRLQFSELPKTISGKIRRVDL
EEEECCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCEEEEHH
RRREMEREDQSSRMPGEYWEEDFPDLR
HHHHHHHHHHHCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKDGASGAAAFVAARDFLLRHRADYDRAYGEFAWPQLGEFNWALDYFDVMAQGNDNPALW
CCCCCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEE
IVDSPNDAGAPLSFREMSERSSRMANYLRGLGVSRGDRLLLMLPNRVELWDVMLAAMKLG
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHC
AVVLPATTQLSPDDVRDRVQLGEARFVVVDGAELGKFEDVDASVTRIAVGERVAGWHHIG
EEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHEEECCCHHCCHHHCC
DAYQASPTFVPDGVTRATDLLLLYFTSGTTSKPKLVEHTHQSYPVGHLSTMYWIGLQPGD
CCCCCCCCCCCCCHHHHHEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEEECCCCC
IHWNISSPGWAKHAWSCFYAPWNAQACVFIYNFARFAPRDALDVLVQRNVTTLCAPPTVW
EEEECCCCCCCCCCCEEEECCCCCCEEEEEEHHHHHCCHHHHHHHHHCCCCEEECCHHHH
RMLVQEPLASYAVKLREIVGAGEPLNPEIIERVQSAWGITIRDGFGQTETTCQIGNPPGQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCEEECCCCCCC
PVVPGSMGRPLPGYRVDLVDLDDHPANEGEIVLSLASRPLGLMAGYSNNEKATAEAMRNG
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHCC
FYHTSDIAMRRDDGYLVYVGRADDVFKASDYRLSPFELESVLIEHEAIGEAAVVPSPDPV
CEECCCEEEEECCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCE
KLYVPKAYVTVRQGYEAGPELARAVFRFSREKLAPYKRIRRLQFSELPKTISGKIRRVDL
EEEECCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCEEEEHH
RRREMEREDQSSRMPGEYWEEDFPDLR
HHHHHHHHHHHCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: Acid-thiol ligation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]