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The map label for this gene is echA8 [H]

Identifier: 94314545

GI number: 94314545

Start: 2382098

End: 2382895

Strand: Reverse

Name: echA8 [H]

Synonym: Rmet_5626

Alternate gene names: 94314545

Gene position: 2382895-2382098 (Counterclockwise)

Preceding gene: 94314546

Following gene: 94314544

Centisome position: 92.36

GC content: 66.79

Gene sequence:

>798_bases
ATGAGCGATGACATTCAGCAGGAAGGGACGTTGCTGGTGACGCGCGAGGATGCCGTCGCCACCATCGTCCTGAACCGGCC
CGCGAAGCTCAATGCCTTCACGCTCGATATGTGGCGGCAGCTTGGCGAGGCGTTCCGCGAGCTTTCCGCCGATGACACGG
TGCGCTGCGTCGTCGTGCGTGGCGCGGGGGACCGCGCGTTTTCGCCGGGCAACGATATCGGCGAATTCGCCACTACGCGT
TCCAATAAGCAACAGGCCACGGCCTATGGCGCGGTCATGCATGGCACCGCGCAGGCGATGCAGGACTGCCCGCACCCGGT
AGTGGCGCAGATCCACGGCATCTGCGTGGGTGGCGGGCTCGAGGTCGCAGCCATGGCCGATATACGCATCTGTGGCCAGA
GCAGCCGTTTCGGCGCGCCGATCAAGAATCTCGGGCTGGTGATGGCGCACGCGGAGATGGCGCCGCTGGTGCGGCTGATC
GGCACGTCGCGCACGCTCGAACTGCTGTTCGAAGGGCGCATCGTCGATGCGGCCGAGGCGTACGCGATGGGGCTGGTCTC
GCGCGTGGTGCCCGATGACCGCGTGGCCGACGAGGCGCGTGCCACGGCGCAGCGGATCGCTTCCGGCGCGCCGCTGGTGG
CCCGTTGGCACAAGCGCTTCGCCCGGCAACTGGCCGAAGGCAAGCCGCTATCGGCCGCCGATCTCGATGCTGCGTTCGAC
TGTTTCGATACCGAGGATTTCCGCACTGGCTATCGCGCGTTCCTGGCCAAGGAGACGCCGAAGTTCAGCGGTCGCTAA

Upstream 100 bases:

>100_bases
TGGCGCTGGCGGTGGTGATCCTGGTCTGGCCGATGTTTGGCAGCCTGCTGGAGAAGACCCGGCGCCGCAAGGTCGTGTCG
GCCCATCAGGGAGCGTCCGA

Downstream 100 bases:

>100_bases
CCTGGGAGCAGACCGATGTCCGATTCCCCTGCAATCGCGCCGGGTGCGTTGTCCGGCCTGCGTGTGCTGGAACTCGCGCA
GATCATGGCCGGGCCCACCT

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSDDIQQEGTLLVTREDAVATIVLNRPAKLNAFTLDMWRQLGEAFRELSADDTVRCVVVRGAGDRAFSPGNDIGEFATTR
SNKQQATAYGAVMHGTAQAMQDCPHPVVAQIHGICVGGGLEVAAMADIRICGQSSRFGAPIKNLGLVMAHAEMAPLVRLI
GTSRTLELLFEGRIVDAAEAYAMGLVSRVVPDDRVADEARATAQRIASGAPLVARWHKRFARQLAEGKPLSAADLDAAFD
CFDTEDFRTGYRAFLAKETPKFSGR

Sequences:

>Translated_265_residues
MSDDIQQEGTLLVTREDAVATIVLNRPAKLNAFTLDMWRQLGEAFRELSADDTVRCVVVRGAGDRAFSPGNDIGEFATTR
SNKQQATAYGAVMHGTAQAMQDCPHPVVAQIHGICVGGGLEVAAMADIRICGQSSRFGAPIKNLGLVMAHAEMAPLVRLI
GTSRTLELLFEGRIVDAAEAYAMGLVSRVVPDDRVADEARATAQRIASGAPLVARWHKRFARQLAEGKPLSAADLDAAFD
CFDTEDFRTGYRAFLAKETPKFSGR
>Mature_264_residues
SDDIQQEGTLLVTREDAVATIVLNRPAKLNAFTLDMWRQLGEAFRELSADDTVRCVVVRGAGDRAFSPGNDIGEFATTRS
NKQQATAYGAVMHGTAQAMQDCPHPVVAQIHGICVGGGLEVAAMADIRICGQSSRFGAPIKNLGLVMAHAEMAPLVRLIG
TSRTLELLFEGRIVDAAEAYAMGLVSRVVPDDRVADEARATAQRIASGAPLVARWHKRFARQLAEGKPLSAADLDAAFDC
FDTEDFRTGYRAFLAKETPKFSGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=29.6442687747036, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI70995211, Length=230, Percent_Identity=33.0434782608696, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=28.4615384615385, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI45643119, Length=216, Percent_Identity=27.3148148148148, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI260275230, Length=216, Percent_Identity=27.3148148148148, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI260274832, Length=216, Percent_Identity=27.3148148148148, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI68989263, Length=200, Percent_Identity=33.5, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI37594471, Length=197, Percent_Identity=27.9187817258883, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594469, Length=147, Percent_Identity=30.6122448979592, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI20127408, Length=203, Percent_Identity=27.0935960591133, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI157694516, Length=221, Percent_Identity=26.2443438914027, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI213417737, Length=221, Percent_Identity=26.2443438914027, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI25453481, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI51538573, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI4757966, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI51538581, Length=215, Percent_Identity=24.6511627906977, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=30.5019305019305, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=27.5590551181102, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=30.1960784313725, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=28.2442748091603, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=27.4193548387097, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=32.1705426356589, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=29.7619047619048, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=231, Percent_Identity=29.8701298701299, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=29.1338582677165, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=27.0531400966184, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=196, Percent_Identity=30.6122448979592, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=261, Percent_Identity=26.4367816091954, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=27.6264591439689, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=237, Percent_Identity=27.0042194092827, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=23.8317757009346, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=26.775956284153, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=25.9067357512953, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=30, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=30, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI19920382, Length=210, Percent_Identity=29.5238095238095, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=33.5164835164835, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=270, Percent_Identity=28.5185185185185, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=25.8823529411765, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24650670, Length=221, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24654903, Length=191, Percent_Identity=28.7958115183246, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28580; Mature: 28449

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDIQQEGTLLVTREDAVATIVLNRPAKLNAFTLDMWRQLGEAFRELSADDTVRCVVVR
CCCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEE
GAGDRAFSPGNDIGEFATTRSNKQQATAYGAVMHGTAQAMQDCPHPVVAQIHGICVGGGL
CCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEECCCC
EVAAMADIRICGQSSRFGAPIKNLGLVMAHAEMAPLVRLIGTSRTLELLFEGRIVDAAEA
EEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEHHCCCCEEHHHHH
YAMGLVSRVVPDDRVADEARATAQRIASGAPLVARWHKRFARQLAEGKPLSAADLDAAFD
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
CFDTEDFRTGYRAFLAKETPKFSGR
CCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDDIQQEGTLLVTREDAVATIVLNRPAKLNAFTLDMWRQLGEAFRELSADDTVRCVVVR
CCCCCCCCCEEEEECCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEEE
GAGDRAFSPGNDIGEFATTRSNKQQATAYGAVMHGTAQAMQDCPHPVVAQIHGICVGGGL
CCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCEEECCCC
EVAAMADIRICGQSSRFGAPIKNLGLVMAHAEMAPLVRLIGTSRTLELLFEGRIVDAAEA
EEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHEEHHCCCCEEHHHHH
YAMGLVSRVVPDDRVADEARATAQRIASGAPLVARWHKRFARQLAEGKPLSAADLDAAFD
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
CFDTEDFRTGYRAFLAKETPKFSGR
CCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]