BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

Click here to switch to the map view.

The map label for this gene is fabG [H]

Identifier: 94314443

GI number: 94314443

Start: 2270648

End: 2271421

Strand: Reverse

Name: fabG [H]

Synonym: Rmet_5524

Alternate gene names: 94314443

Gene position: 2271421-2270648 (Counterclockwise)

Preceding gene: 94314445

Following gene: 94314442

Centisome position: 88.04

GC content: 59.43

Gene sequence:

>774_bases
ATGTCGCAAATCGTTTCGTTGGAAGGTCGCACCATCGTGGTTACGGGCGCTGCACAGGGTATCGGCAAGGCAGTTGTCGA
ACTCGGCATTGAGCTCGGCGCAAACGTTGTCGCCGTGGATCTGAACGGTGAAACGCTGAACGCTGCCCTCGCTCCGTTGC
CCGCGGAGCGCGTGATGGCCGTCGCAGGCAGCGTCGCAGATTCGGCGCTTGCGACCCGCGCAGTGGCTGACGCTGTGTCG
CGATTCGGCGCCGTTCACGGTCTCGTCAACAATGCCGGCATCATCCGTCCTGCAATGATCGATAAGATGACTGACCAGCA
ATGGACGGATGTCATCGATGTACATCTGAGCGGTTCGTTCTACTGGACCCGCGCTGTTGGTCAACACATGGTCTCGCGCG
CAAAGGACGGTGACAAGACCGGCGGCTCGATCGTCAACATTTCGTCTGACGCAGGGCGCAAGGGCTCGATTGGGCAAATC
AACTATGCAGCGGCAAAGGCAGGCATGCTCGGAATGACGATGACCGCCGCTCGCGAATGGGGCCGCTACAACATCCGTTC
CAACTCCGTCTGCTTTGGCGTGGTTGAAACGCCTATGACGGAAGTCGTTCGCGGCGAGAAGTTCCGCGACGGCATGCTCG
CGCAAATCCCCCTGGGCCGCTGGTCGACTCCCGAAGAGGTCGTGAAGACTGTTTGTTTCCTGCTTTCTGACGGTTCAAGC
TACGTTACGGGCCAACACCTCGGGGTTAACGGCGGATTCCACATCAGCCTCTAA

Upstream 100 bases:

>100_bases
GTGCGCCCTAGACGCTTTCGTTGCGCCCGTTCTATTTCCCATTGACGCCGATCCACTTCCAAAATATCATTGGTTCAACC
AAACTAAAAGGGGTACGGAG

Downstream 100 bases:

>100_bases
TCATGGACGCCGGTGCCGTCTTTCATGAGGACCCCAGCGTAGGCGAGCGGCTGGCCGCCTATATCCGCGGCCAAGTCGCG
CCGTTCGCCGGCGCGTTGGA

Product: 3-oxoacyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: 3-ketoacyl-acyl carrier protein reductase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSQIVSLEGRTIVVTGAAQGIGKAVVELGIELGANVVAVDLNGETLNAALAPLPAERVMAVAGSVADSALATRAVADAVS
RFGAVHGLVNNAGIIRPAMIDKMTDQQWTDVIDVHLSGSFYWTRAVGQHMVSRAKDGDKTGGSIVNISSDAGRKGSIGQI
NYAAAKAGMLGMTMTAAREWGRYNIRSNSVCFGVVETPMTEVVRGEKFRDGMLAQIPLGRWSTPEEVVKTVCFLLSDGSS
YVTGQHLGVNGGFHISL

Sequences:

>Translated_257_residues
MSQIVSLEGRTIVVTGAAQGIGKAVVELGIELGANVVAVDLNGETLNAALAPLPAERVMAVAGSVADSALATRAVADAVS
RFGAVHGLVNNAGIIRPAMIDKMTDQQWTDVIDVHLSGSFYWTRAVGQHMVSRAKDGDKTGGSIVNISSDAGRKGSIGQI
NYAAAKAGMLGMTMTAAREWGRYNIRSNSVCFGVVETPMTEVVRGEKFRDGMLAQIPLGRWSTPEEVVKTVCFLLSDGSS
YVTGQHLGVNGGFHISL
>Mature_256_residues
SQIVSLEGRTIVVTGAAQGIGKAVVELGIELGANVVAVDLNGETLNAALAPLPAERVMAVAGSVADSALATRAVADAVSR
FGAVHGLVNNAGIIRPAMIDKMTDQQWTDVIDVHLSGSFYWTRAVGQHMVSRAKDGDKTGGSIVNISSDAGRKGSIGQIN
YAAAKAGMLGMTMTAAREWGRYNIRSNSVCFGVVETPMTEVVRGEKFRDGMLAQIPLGRWSTPEEVVKTVCFLLSDGSSY
VTGQHLGVNGGFHISL

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=257, Percent_Identity=40.8560311284047, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=34.7490347490347, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=32.6612903225806, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI4504505, Length=235, Percent_Identity=32.3404255319149, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI7705925, Length=248, Percent_Identity=31.0483870967742, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32483357, Length=258, Percent_Identity=30.6201550387597, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI4758504, Length=257, Percent_Identity=29.1828793774319, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4503301, Length=254, Percent_Identity=26.7716535433071, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI66933014, Length=257, Percent_Identity=29.1828793774319, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI59889578, Length=188, Percent_Identity=30.8510638297872, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI31542939, Length=213, Percent_Identity=31.4553990610329, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI10190704, Length=255, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI83715985, Length=257, Percent_Identity=28.4046692607004, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=251, Percent_Identity=28.6852589641434, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI224922801, Length=181, Percent_Identity=32.0441988950276, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787335, Length=253, Percent_Identity=37.1541501976285, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789208, Length=254, Percent_Identity=31.496062992126, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=32.3076923076923, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=34.9019607843137, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786812, Length=262, Percent_Identity=30.1526717557252, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=30.46875, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788459, Length=202, Percent_Identity=31.6831683168317, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI2367175, Length=254, Percent_Identity=33.0708661417323, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=30.4347826086957, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789057, Length=248, Percent_Identity=28.6290322580645, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789378, Length=258, Percent_Identity=26.3565891472868, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI2367365, Length=252, Percent_Identity=27.3809523809524, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=250, Percent_Identity=39.6, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17555706, Length=247, Percent_Identity=37.6518218623482, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=32.3076923076923, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560676, Length=255, Percent_Identity=30.5882352941176, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=35.0806451612903, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568967, Length=239, Percent_Identity=29.7071129707113, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17560332, Length=268, Percent_Identity=31.7164179104478, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17536651, Length=266, Percent_Identity=28.1954887218045, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562990, Length=269, Percent_Identity=28.996282527881, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=260, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=28.1954887218045, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=252, Percent_Identity=28.968253968254, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17538182, Length=264, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=25.1908396946565, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=278, Percent_Identity=28.4172661870504, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=27.1698113207547, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=29.296875, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=268, Percent_Identity=27.6119402985075, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=273, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=268, Percent_Identity=25.3731343283582, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=264, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17561272, Length=196, Percent_Identity=26.530612244898, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=272, Percent_Identity=26.1029411764706, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=234, Percent_Identity=29.9145299145299, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17563726, Length=262, Percent_Identity=29.0076335877863, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=268, Percent_Identity=27.2388059701493, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=231, Percent_Identity=29.8701298701299, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=262, Percent_Identity=27.0992366412214, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=202, Percent_Identity=27.7227722772277, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=263, Percent_Identity=28.5171102661597, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=259, Percent_Identity=33.5907335907336, Blast_Score=115, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324126, Length=253, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322226, Length=198, Percent_Identity=26.7676767676768, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=36.144578313253, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=35.5731225296443, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI18859837, Length=250, Percent_Identity=34.8, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI23397609, Length=256, Percent_Identity=31.25, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355319, Length=252, Percent_Identity=28.1746031746032, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24644339, Length=260, Percent_Identity=29.6153846153846, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357041, Length=264, Percent_Identity=30.3030303030303, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571526, Length=265, Percent_Identity=29.811320754717, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17737361, Length=221, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24644337, Length=265, Percent_Identity=28.6792452830189, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011284
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.100 [H]

Molecular weight: Translated: 26932; Mature: 26801

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIVSLEGRTIVVTGAAQGIGKAVVELGIELGANVVAVDLNGETLNAALAPLPAERVMA
CCCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHCCCCHHHHHH
VAGSVADSALATRAVADAVSRFGAVHGLVNNAGIIRPAMIDKMTDQQWTDVIDVHLSGSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHCEEEEEEECCCE
YWTRAVGQHMVSRAKDGDKTGGSIVNISSDAGRKGSIGQINYAAAKAGMLGMTMTAAREW
EEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHC
GRYNIRSNSVCFGVVETPMTEVVRGEKFRDGMLAQIPLGRWSTPEEVVKTVCFLLSDGSS
CCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCC
YVTGQHLGVNGGFHISL
EEECCEEECCCCEEEEC
>Mature Secondary Structure 
SQIVSLEGRTIVVTGAAQGIGKAVVELGIELGANVVAVDLNGETLNAALAPLPAERVMA
CCEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHCCCCHHHHHH
VAGSVADSALATRAVADAVSRFGAVHGLVNNAGIIRPAMIDKMTDQQWTDVIDVHLSGSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHCEEEEEEECCCE
YWTRAVGQHMVSRAKDGDKTGGSIVNISSDAGRKGSIGQINYAAAKAGMLGMTMTAAREW
EEHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEHHHHHCCHHHHHHHHHHHC
GRYNIRSNSVCFGVVETPMTEVVRGEKFRDGMLAQIPLGRWSTPEEVVKTVCFLLSDGSS
CCCCCCCCCEEEEEECCCHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCC
YVTGQHLGVNGGFHISL
EEECCEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]