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The map label for this gene is paaF [C]

Identifier: 94314424

GI number: 94314424

Start: 2249197

End: 2250078

Strand: Direct

Name: paaF [C]

Synonym: Rmet_5505

Alternate gene names: 94314424

Gene position: 2249197-2250078 (Clockwise)

Preceding gene: 291481580

Following gene: 94314428

Centisome position: 87.18

GC content: 57.03

Gene sequence:

>882_bases
GTGCGCAAGTGGCTTAGAACAACCGAGAGCATCGAGTTCGCCGTCAAAGGCCGTGTTGCATACGTGACCCTAAACAGGCC
AGAATGCCGCAATGCACTGTCCCCCGAACTCCTCGCTGAGTACCGGTCAGCCCTGCTTGAAGCAGATGATCTCCGGGCTG
TAAGTGCTATCGTCGTTGCTGGAAACGGCAAGGATTTTTGTGCCGGCTATGACATGAAGGGAGCCTATGCCCGTTACGCA
TCCGAGCACGGCGATGAAGCGAGCCCTTATCGAACCGGAATCGGTTCTTTTGACGATGACTCCTGGAAGCTGGAACGCTC
CCAGGAAATGCTGCGGACCCCCTTTGACATTCATAAACCTGTCATTGCGAAGGTTCACGGGAACTGCGTCGCGGGTGGGA
CCGACTTAGCTCTATACTGCGACATGATCATCGCAGCCGACAACGCCCGCATTGGGTTTCCCGCGACACGTGCAATGGGC
TCCCCGCCCAACCACATGTGGATCTACAACGTCGGCCCGCAATGGGCGAAAAGGCTATTGCTAACTGGCGACGTACTTTT
AGGCAAGGATGCAGCAAAGATCGGCCTTGCTGTGAGTTCGATCCCCGAAAGCGACCTCGACCAAGAAGTGGAGCGACTTG
CCAACAGCATTGGTTCGCTGGACCCTGAGCTGCTTGCCTGCAACAAGCGAATCGTTAACCTGGCTCTCGAAGTTGGCGGC
GCCTCTGTGCTTCAGCGCCTGGCTGCCGAAATGGACGCGAGAGCCCATCTCACACGTGCCAAGGCCGAATTTGACGAAGA
TGTTCGTGCCCTTGGATTCAAGGGTGCAGCAGAGAAGCGCGACAAGCCCTTCGGTGACGGCGTCGCAAGGCTGAGTTGGT
AG

Upstream 100 bases:

>100_bases
AGAGTCAGTTGTTTCGGTACCTCTTTTGGCTCGTAGTCAGGCGTTCTGCCTGACGAACTCACATTGAGACTATTCAACTG
AATCGCGAGGAGACCTGATA

Downstream 100 bases:

>100_bases
TCGCTGCGTACGCACCAGCGAACAGGTAGCCCAAGATTCTAGAAGCTCCTGACGACGGCGCCAACCGCCCCGTCCCGCGG
TACGGACGCCATCGTCTGTA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MRKWLRTTESIEFAVKGRVAYVTLNRPECRNALSPELLAEYRSALLEADDLRAVSAIVVAGNGKDFCAGYDMKGAYARYA
SEHGDEASPYRTGIGSFDDDSWKLERSQEMLRTPFDIHKPVIAKVHGNCVAGGTDLALYCDMIIAADNARIGFPATRAMG
SPPNHMWIYNVGPQWAKRLLLTGDVLLGKDAAKIGLAVSSIPESDLDQEVERLANSIGSLDPELLACNKRIVNLALEVGG
ASVLQRLAAEMDARAHLTRAKAEFDEDVRALGFKGAAEKRDKPFGDGVARLSW

Sequences:

>Translated_293_residues
MRKWLRTTESIEFAVKGRVAYVTLNRPECRNALSPELLAEYRSALLEADDLRAVSAIVVAGNGKDFCAGYDMKGAYARYA
SEHGDEASPYRTGIGSFDDDSWKLERSQEMLRTPFDIHKPVIAKVHGNCVAGGTDLALYCDMIIAADNARIGFPATRAMG
SPPNHMWIYNVGPQWAKRLLLTGDVLLGKDAAKIGLAVSSIPESDLDQEVERLANSIGSLDPELLACNKRIVNLALEVGG
ASVLQRLAAEMDARAHLTRAKAEFDEDVRALGFKGAAEKRDKPFGDGVARLSW
>Mature_293_residues
MRKWLRTTESIEFAVKGRVAYVTLNRPECRNALSPELLAEYRSALLEADDLRAVSAIVVAGNGKDFCAGYDMKGAYARYA
SEHGDEASPYRTGIGSFDDDSWKLERSQEMLRTPFDIHKPVIAKVHGNCVAGGTDLALYCDMIIAADNARIGFPATRAMG
SPPNHMWIYNVGPQWAKRLLLTGDVLLGKDAAKIGLAVSSIPESDLDQEVERLANSIGSLDPELLACNKRIVNLALEVGG
ASVLQRLAAEMDARAHLTRAKAEFDEDVRALGFKGAAEKRDKPFGDGVARLSW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=228, Percent_Identity=30.2631578947368, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=29.3650793650794, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI221142681, Length=236, Percent_Identity=26.6949152542373, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=227, Percent_Identity=30.8370044052863, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=269, Percent_Identity=32.3420074349442, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=250, Percent_Identity=26.8, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=235, Percent_Identity=22.9787234042553, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20129971, Length=281, Percent_Identity=31.6725978647687, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653477, Length=281, Percent_Identity=31.6725978647687, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 31968; Mature: 31968

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKWLRTTESIEFAVKGRVAYVTLNRPECRNALSPELLAEYRSALLEADDLRAVSAIVVA
CCCHHHCCCCEEEEEECEEEEEEECCHHHHCCCCHHHHHHHHHHHHCHHHHHEEEEEEEE
GNGKDFCAGYDMKGAYARYASEHGDEASPYRTGIGSFDDDSWKLERSQEMLRTPFDIHKP
CCCCCEECCCCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCHHHCCC
VIAKVHGNCVAGGTDLALYCDMIIAADNARIGFPATRAMGSPPNHMWIYNVGPQWAKRLL
HHEEECCCEEECCCHHHHHHEEHEEECCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHH
LTGDVLLGKDAAKIGLAVSSIPESDLDQEVERLANSIGSLDPELLACNKRIVNLALEVGG
HCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
ASVLQRLAAEMDARAHLTRAKAEFDEDVRALGFKGAAEKRDKPFGDGVARLSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKWLRTTESIEFAVKGRVAYVTLNRPECRNALSPELLAEYRSALLEADDLRAVSAIVVA
CCCHHHCCCCEEEEEECEEEEEEECCHHHHCCCCHHHHHHHHHHHHCHHHHHEEEEEEEE
GNGKDFCAGYDMKGAYARYASEHGDEASPYRTGIGSFDDDSWKLERSQEMLRTPFDIHKP
CCCCCEECCCCCCHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHCCHHHCCC
VIAKVHGNCVAGGTDLALYCDMIIAADNARIGFPATRAMGSPPNHMWIYNVGPQWAKRLL
HHEEECCCEEECCCHHHHHHEEHEEECCCCCCCCHHHHCCCCCCEEEEEECCHHHHHHHH
LTGDVLLGKDAAKIGLAVSSIPESDLDQEVERLANSIGSLDPELLACNKRIVNLALEVGG
HCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
ASVLQRLAAEMDARAHLTRAKAEFDEDVRALGFKGAAEKRDKPFGDGVARLSW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA