Click here to switch to the map view.

The map label for this gene is paaG [C]

Identifier: 94314420

GI number: 94314420

Start: 2244418

End: 2245212

Strand: Direct

Name: paaG [C]

Synonym: Rmet_5501

Alternate gene names: 94314420

Gene position: 2244418-2245212 (Clockwise)

Preceding gene: 94314419

Following gene: 94314421

Centisome position: 86.99

GC content: 57.86

Gene sequence:

>795_bases
ATGGATACTCTCGACTTCGAAGTAGTGAACAACGTTGGCTGGATTCGCATGAATCGGGCCGCGAAGCACAATCCGTTTGA
TGCGGAGCTTCGCGCCGACCTGATGACCGTCTTGGAGCGTGTTCGAGACGATGCTGACATCAGGGTCCTCGTGCTTACGA
GCCATCCTGGCTCATTCTGCGCGGGAGGGAATCTCCATGTTCTTCGAGACAACCTCGACTCTGGCCCCGCCTACTGGCAG
CAGCGCATCAAGACCGGCCTTCGATTCATCCACGATATGTTGAATCTCGGTCGCCCAGTTATTGCTGCCGTAGACGGCCC
GGCATTCGGAGCTGGATTCGCGTTGTCTCTGACAGCAGATATCGTGCTGGCAAGTCCTCGTGCACGCTTCTCTATGGCCT
ACCTCCGCCTTGGTCTGGTACCGGATCTCGGCGCGCTGTACCTTCTTCCCCGAGCAGTTGGACTGCAACGAGCCAAGGAG
TTGATGTTCTCAACCCGAGAACTGGACGCGGAAGAGGCTCATCGCCTGGGGCTTGTGATGGAAGTACATGAGAGCGAGGC
ACTTGAGCAGCGTGCTCGAGAAATTGCCGAGAGCCTGGTCCAAGCTGCCCCGACCGCCCTCGCACTGACGAAGGCGGCGC
TGAACGTTTCGTTGGATTCCGATGAGCAAACGATGTTTAGTCTCGAGGCGGCCAGCCAAGCTGCAGCTTTCTCGACGAAG
GAACCGCGCGCTGCAATCGAAGCACTCCTGTCCAAACAACCTCCTCCGTTTCGCGGATTTCCACGGCGTTCGTAA

Upstream 100 bases:

>100_bases
TGGATTCGCTGAAGATCGGACAGCGCGTGAAGGCTTGCTTCAAGCCTTCCGAAGGAGACAGTCCAATCCCGATGTTCACA
CCTGCTTAAAGCGAGGGAAC

Downstream 100 bases:

>100_bases
TCATGCGATCTCTCAAGAACAAGACGCTATTCGTCACCGGGGCGAGTCGCGGCATCGGCCTGGCAATCGCAAAGCGGGCG
GCTCAAGATGGTGCGAACAT

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDTLDFEVVNNVGWIRMNRAAKHNPFDAELRADLMTVLERVRDDADIRVLVLTSHPGSFCAGGNLHVLRDNLDSGPAYWQ
QRIKTGLRFIHDMLNLGRPVIAAVDGPAFGAGFALSLTADIVLASPRARFSMAYLRLGLVPDLGALYLLPRAVGLQRAKE
LMFSTRELDAEEAHRLGLVMEVHESEALEQRAREIAESLVQAAPTALALTKAALNVSLDSDEQTMFSLEAASQAAAFSTK
EPRAAIEALLSKQPPPFRGFPRRS

Sequences:

>Translated_264_residues
MDTLDFEVVNNVGWIRMNRAAKHNPFDAELRADLMTVLERVRDDADIRVLVLTSHPGSFCAGGNLHVLRDNLDSGPAYWQ
QRIKTGLRFIHDMLNLGRPVIAAVDGPAFGAGFALSLTADIVLASPRARFSMAYLRLGLVPDLGALYLLPRAVGLQRAKE
LMFSTRELDAEEAHRLGLVMEVHESEALEQRAREIAESLVQAAPTALALTKAALNVSLDSDEQTMFSLEAASQAAAFSTK
EPRAAIEALLSKQPPPFRGFPRRS
>Mature_264_residues
MDTLDFEVVNNVGWIRMNRAAKHNPFDAELRADLMTVLERVRDDADIRVLVLTSHPGSFCAGGNLHVLRDNLDSGPAYWQ
QRIKTGLRFIHDMLNLGRPVIAAVDGPAFGAGFALSLTADIVLASPRARFSMAYLRLGLVPDLGALYLLPRAVGLQRAKE
LMFSTRELDAEEAHRLGLVMEVHESEALEQRAREIAESLVQAAPTALALTKAALNVSLDSDEQTMFSLEAASQAAAFSTK
EPRAAIEALLSKQPPPFRGFPRRS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=32.258064516129, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=27.710843373494, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI150378541, Length=250, Percent_Identity=27.6, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=202, Percent_Identity=24.7524752475248, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221136753, Length=202, Percent_Identity=24.7524752475248, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI221307494, Length=202, Percent_Identity=27.2277227722772, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=204, Percent_Identity=28.921568627451, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260275230, Length=204, Percent_Identity=28.921568627451, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260274832, Length=204, Percent_Identity=28.921568627451, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI116235487, Length=190, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4757966, Length=205, Percent_Identity=27.3170731707317, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538581, Length=205, Percent_Identity=27.3170731707317, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI25453481, Length=205, Percent_Identity=27.3170731707317, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI51538573, Length=205, Percent_Identity=27.3170731707317, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI70995211, Length=244, Percent_Identity=27.0491803278689, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI4757968, Length=169, Percent_Identity=28.9940828402367, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=169, Percent_Identity=28.9940828402367, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=24.5901639344262, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI20127408, Length=271, Percent_Identity=23.6162361623616, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=29.9465240641711, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=36.1867704280156, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI221142681, Length=195, Percent_Identity=35.3846153846154, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=30.2325581395349, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=27.9693486590038, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=28.0632411067194, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=25.7731958762887, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=27.8409090909091, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=225, Percent_Identity=32.8888888888889, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=29.2237442922374, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=25.9109311740891, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=28.7449392712551, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=223, Percent_Identity=27.3542600896861, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=246, Percent_Identity=25.2032520325203, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=270, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=214, Percent_Identity=27.1028037383178, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=238, Percent_Identity=26.890756302521, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=208, Percent_Identity=25.9615384615385, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=30.5882352941176, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=29.6, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=29.6, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920382, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24654903, Length=174, Percent_Identity=28.1609195402299, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28943; Mature: 28943

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLDFEVVNNVGWIRMNRAAKHNPFDAELRADLMTVLERVRDDADIRVLVLTSHPGSFC
CCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
AGGNLHVLRDNLDSGPAYWQQRIKTGLRFIHDMLNLGRPVIAAVDGPAFGAGFALSLTAD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEE
IVLASPRARFSMAYLRLGLVPDLGALYLLPRAVGLQRAKELMFSTRELDAEEAHRLGLVM
EEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHE
EVHESEALEQRAREIAESLVQAAPTALALTKAALNVSLDSDEQTMFSLEAASQAAAFSTK
EHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCC
EPRAAIEALLSKQPPPFRGFPRRS
CHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDTLDFEVVNNVGWIRMNRAAKHNPFDAELRADLMTVLERVRDDADIRVLVLTSHPGSFC
CCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
AGGNLHVLRDNLDSGPAYWQQRIKTGLRFIHDMLNLGRPVIAAVDGPAFGAGFALSLTAD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEE
IVLASPRARFSMAYLRLGLVPDLGALYLLPRAVGLQRAKELMFSTRELDAEEAHRLGLVM
EEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHE
EVHESEALEQRAREIAESLVQAAPTALALTKAALNVSLDSDEQTMFSLEAASQAAAFSTK
EHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCC
EPRAAIEALLSKQPPPFRGFPRRS
CHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA