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The map label for this gene is rep [H]

Identifier: 94314406

GI number: 94314406

Start: 2228957

End: 2230732

Strand: Reverse

Name: rep [H]

Synonym: Rmet_5487

Alternate gene names: 94314406

Gene position: 2230732-2228957 (Counterclockwise)

Preceding gene: 94314407

Following gene: 94314405

Centisome position: 86.46

GC content: 57.66

Gene sequence:

>1776_bases
GTGTCTAATCGCGCCGCCTATCTTCAAGCTGCCGAGGAGCTTCGCGACAATCCTGGGCAGTGGGCAGCTTACGAGTCGCG
AGGACACTGTGTAGTCTTGGCTGGTCCTGGTAGCGGCAAGACGAAGACTCTCACTACCAAGATGGCACGGCTTCTCGCAG
AGGACGTAGCCGAGCCGCGGGGTGTCGCATGCATTACGTACAACAACGAGTGTGCTCGTGAGCTAGAGGCACGACTTTCT
TCGCTAGGCGTAGAGCCAGGCGGGCGTGTTTTCGTAGGAACTGTGCACTCATTCTCGCTTACGCAGGTGATCTTGCCCTA
CGCGAAGGTGGCGGGTTTGGGATTACCAGATGACTTCCGTGTCGCCACCAATGCGGAAGCGCTTGCGGCTCTGGACGATG
CGGCCCAAGTTGCCATGGGTGCGGTTCCCAATCTGCAAACCATACGCTCGCAAATGGGGAGCTATCGTCGCTCAATCCTT
AATCGGGACAGCGACCTATGGCGGGATACGAATCCTCGTTTGGCAAGGTTGGCTGAGGCCTATGAGGCCGAGCTCCGCGT
TCGTGGGCTCATTGACTTCGATGACATGCCTCTTCTCGCAGTTCGTGCGCTGAGAGAGAACCCTTGGCTTCAGACAGCAT
TGCTTGCGAAATACCCTGTCCTGGTCGTTGACGAGTATCAGGACCTCGGTGCGGCTCTGCACCGCATGGTTATGGGCCTC
TGCTTTAGTGCTGGTATGCGGCTTTTCGCAGTGGGAGATGTCGACCAGTCGATTTACGGCTTCATTGGAGCTCAGCCGCA
AATGTTGCAGCGTCTGGCCGAGAGGGAGGATGTGGAGATCATTCGCCTTCAGCTCAATTATCGGAGCGGACATCGAATCG
TGGCCGCGTCTGGCGCTGTTCTTGGGGTGGATCGCGGGTACGCGGCGGTGGACGGGGCGCCGCAGGGTGAAATTTTCTTC
CATTCCCTCGGGGGTAGCTACGAGGTGCAGGCTCGTCGTCTCATGACCGACCTCCTTCCCGCCGCTATGGCGCGTGAGCC
AGGTGTTCGCTACGACGAAATTGCCGTCTTGTATCCCGCCGCCTGGATTGGCAATGCTGTCGCAGAGGCGGCGCGCCACG
CCGGAGTTCCGTTTATTCGGACGGATGGTAACGCTCTATATCCCAGGTCGAGCCGGTTGATGCAATGGTTGGAACTGAGC
GCTCAATGGTGTTGCGGAGGGTGGCGGACTGGTAGCCCACGGTTTTCCCAGTTGGTATCGGAGGGGCGAAGGCTGTTCGG
GGACGCCATCCGAGCAGAATTGGACGCAGCGGCATTTCATCGAGCGCTAGTGGCTTGTCTATGGGAGATGCGAAGACCCG
ACATGGTGCTCTATGACTGGCTACAGCGATTCAAGCGCACCGTGTTGGACCAACATGCCCAACTATCGCCGAACCTGAGG
GATGAGCTCAACATCCTGGGTTCGTTTCTGGAGAGAGTCTCGCCGGATGCCGACTACGCGGAGATGACTGTTGCGGAGTT
TGCCGGCGAACATACATTCGACAGGCTGATGCTGTCCACGCTTCACAGCGCAAAGGGCCGAGAGTTTCGCTTCGTATTTC
TGTTTGGGATTGATCACGGGCGATTGCCACGGAATGGCGCCAGACCTGATGAGATCAATGAAAGTCGTCGGCTATTCTAT
GTGGGCATAAGCCGAGCAAGAACAGAGGTCCACATTGCCTACACACACGGCAACTCGTCAATCTTCGTGGATGAGCTTCA
AGCGCGGTTGAACTGA

Upstream 100 bases:

>100_bases
GGTGTCGGAAAGGGGCGTTTTGCCCAGAGGCTAGCGTCAAAGATTGTGGGTGCCATCCCTCCTGAATACATATCTAAAGC
GATCTCCTACGTAGTCAATC

Downstream 100 bases:

>100_bases
CCAGAGCAGCCCTTTATGCGCAGCCAGATTGCTCCGGTGCGCAACGGTTGCCCGAAGACCGAAACGATATCAGACGAAGA
TTCGGCACGCTGTACGAGCA

Product: putative ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSNRAAYLQAAEELRDNPGQWAAYESRGHCVVLAGPGSGKTKTLTTKMARLLAEDVAEPRGVACITYNNECARELEARLS
SLGVEPGGRVFVGTVHSFSLTQVILPYAKVAGLGLPDDFRVATNAEALAALDDAAQVAMGAVPNLQTIRSQMGSYRRSIL
NRDSDLWRDTNPRLARLAEAYEAELRVRGLIDFDDMPLLAVRALRENPWLQTALLAKYPVLVVDEYQDLGAALHRMVMGL
CFSAGMRLFAVGDVDQSIYGFIGAQPQMLQRLAEREDVEIIRLQLNYRSGHRIVAASGAVLGVDRGYAAVDGAPQGEIFF
HSLGGSYEVQARRLMTDLLPAAMAREPGVRYDEIAVLYPAAWIGNAVAEAARHAGVPFIRTDGNALYPRSSRLMQWLELS
AQWCCGGWRTGSPRFSQLVSEGRRLFGDAIRAELDAAAFHRALVACLWEMRRPDMVLYDWLQRFKRTVLDQHAQLSPNLR
DELNILGSFLERVSPDADYAEMTVAEFAGEHTFDRLMLSTLHSAKGREFRFVFLFGIDHGRLPRNGARPDEINESRRLFY
VGISRARTEVHIAYTHGNSSIFVDELQARLN

Sequences:

>Translated_591_residues
MSNRAAYLQAAEELRDNPGQWAAYESRGHCVVLAGPGSGKTKTLTTKMARLLAEDVAEPRGVACITYNNECARELEARLS
SLGVEPGGRVFVGTVHSFSLTQVILPYAKVAGLGLPDDFRVATNAEALAALDDAAQVAMGAVPNLQTIRSQMGSYRRSIL
NRDSDLWRDTNPRLARLAEAYEAELRVRGLIDFDDMPLLAVRALRENPWLQTALLAKYPVLVVDEYQDLGAALHRMVMGL
CFSAGMRLFAVGDVDQSIYGFIGAQPQMLQRLAEREDVEIIRLQLNYRSGHRIVAASGAVLGVDRGYAAVDGAPQGEIFF
HSLGGSYEVQARRLMTDLLPAAMAREPGVRYDEIAVLYPAAWIGNAVAEAARHAGVPFIRTDGNALYPRSSRLMQWLELS
AQWCCGGWRTGSPRFSQLVSEGRRLFGDAIRAELDAAAFHRALVACLWEMRRPDMVLYDWLQRFKRTVLDQHAQLSPNLR
DELNILGSFLERVSPDADYAEMTVAEFAGEHTFDRLMLSTLHSAKGREFRFVFLFGIDHGRLPRNGARPDEINESRRLFY
VGISRARTEVHIAYTHGNSSIFVDELQARLN
>Mature_590_residues
SNRAAYLQAAEELRDNPGQWAAYESRGHCVVLAGPGSGKTKTLTTKMARLLAEDVAEPRGVACITYNNECARELEARLSS
LGVEPGGRVFVGTVHSFSLTQVILPYAKVAGLGLPDDFRVATNAEALAALDDAAQVAMGAVPNLQTIRSQMGSYRRSILN
RDSDLWRDTNPRLARLAEAYEAELRVRGLIDFDDMPLLAVRALRENPWLQTALLAKYPVLVVDEYQDLGAALHRMVMGLC
FSAGMRLFAVGDVDQSIYGFIGAQPQMLQRLAEREDVEIIRLQLNYRSGHRIVAASGAVLGVDRGYAAVDGAPQGEIFFH
SLGGSYEVQARRLMTDLLPAAMAREPGVRYDEIAVLYPAAWIGNAVAEAARHAGVPFIRTDGNALYPRSSRLMQWLELSA
QWCCGGWRTGSPRFSQLVSEGRRLFGDAIRAELDAAAFHRALVACLWEMRRPDMVLYDWLQRFKRTVLDQHAQLSPNLRD
ELNILGSFLERVSPDADYAEMTVAEFAGEHTFDRLMLSTLHSAKGREFRFVFLFGIDHGRLPRNGARPDEINESRRLFYV
GISRARTEVHIAYTHGNSSIFVDELQARLN

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=628, Percent_Identity=26.1146496815287, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI2367296, Length=623, Percent_Identity=25.6821829855538, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322369, Length=342, Percent_Identity=26.0233918128655, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 65690; Mature: 65558

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRAAYLQAAEELRDNPGQWAAYESRGHCVVLAGPGSGKTKTLTTKMARLLAEDVAEPR
CCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
GVACITYNNECARELEARLSSLGVEPGGRVFVGTVHSFSLTQVILPYAKVAGLGLPDDFR
CEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCE
VATNAEALAALDDAAQVAMGAVPNLQTIRSQMGSYRRSILNRDSDLWRDTNPRLARLAEA
EECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHH
YEAELRVRGLIDFDDMPLLAVRALRENPWLQTALLAKYPVLVVDEYQDLGAALHRMVMGL
HHHHEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHHH
CFSAGMRLFAVGDVDQSIYGFIGAQPQMLQRLAEREDVEIIRLQLNYRSGHRIVAASGAV
HHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCE
LGVDRGYAAVDGAPQGEIFFHSLGGSYEVQARRLMTDLLPAAMAREPGVRYDEIAVLYPA
EEECCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECH
AWIGNAVAEAARHAGVPFIRTDGNALYPRSSRLMQWLELSAQWCCGGWRTGSPRFSQLVS
HHHHHHHHHHHHHCCCCEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EGRRLFGDAIRAELDAAAFHRALVACLWEMRRPDMVLYDWLQRFKRTVLDQHAQLSPNLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
DELNILGSFLERVSPDADYAEMTVAEFAGEHTFDRLMLSTLHSAKGREFRFVFLFGIDHG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
RLPRNGARPDEINESRRLFYVGISRARTEVHIAYTHGNSSIFVDELQARLN
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCEEHHHHHHHCC
>Mature Secondary Structure 
SNRAAYLQAAEELRDNPGQWAAYESRGHCVVLAGPGSGKTKTLTTKMARLLAEDVAEPR
CCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
GVACITYNNECARELEARLSSLGVEPGGRVFVGTVHSFSLTQVILPYAKVAGLGLPDDFR
CEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCE
VATNAEALAALDDAAQVAMGAVPNLQTIRSQMGSYRRSILNRDSDLWRDTNPRLARLAEA
EECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHH
YEAELRVRGLIDFDDMPLLAVRALRENPWLQTALLAKYPVLVVDEYQDLGAALHRMVMGL
HHHHEEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHCCEEEEECHHHHHHHHHHHHHHH
CFSAGMRLFAVGDVDQSIYGFIGAQPQMLQRLAEREDVEIIRLQLNYRSGHRIVAASGAV
HHHCCCEEEEECCCCHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEECCCE
LGVDRGYAAVDGAPQGEIFFHSLGGSYEVQARRLMTDLLPAAMAREPGVRYDEIAVLYPA
EEECCCCEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECH
AWIGNAVAEAARHAGVPFIRTDGNALYPRSSRLMQWLELSAQWCCGGWRTGSPRFSQLVS
HHHHHHHHHHHHHCCCCEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
EGRRLFGDAIRAELDAAAFHRALVACLWEMRRPDMVLYDWLQRFKRTVLDQHAQLSPNLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
DELNILGSFLERVSPDADYAEMTVAEFAGEHTFDRLMLSTLHSAKGREFRFVFLFGIDHG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
RLPRNGARPDEINESRRLFYVGISRARTEVHIAYTHGNSSIFVDELQARLN
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]