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The map label for this gene is zneB [H]

Identifier: 94314249

GI number: 94314249

Start: 2042082

End: 2043239

Strand: Reverse

Name: zneB [H]

Synonym: Rmet_5330

Alternate gene names: 94314249

Gene position: 2043239-2042082 (Counterclockwise)

Preceding gene: 94314252

Following gene: 94314248

Centisome position: 79.19

GC content: 65.72

Gene sequence:

>1158_bases
ATGAAAAACAAGCCAGCCTTTCCCGGGCGCGTTGTCTACTGGCTTGCCGCGGCGGTCATACTGGGTCTCGGCGGTGCTGG
TGTCTGGACGATGCGCGCCAAGGCCGAACAGAAGCGCGCGGACCCGCCCGTCGCCCTGCGTCATGAAGGCGAGCGCCTGG
TAGTCCCGGCCGAGTCGCCATTGCGCCGCACCTTGGCCGTCGCGCCCGCCACGCGCGAAACCGTGGCTGCACCGTTCAAT
CTGCCGGCCATGATCGAAGCCGATCCGGCCAAGCTCGTGAAAGTGCTGCCGCCGCTGGCCGGCCGTATCGTCAGCCTGAA
CAAGCAGCTTGGCGACGAGGTCAAGGCTGGCGACGTGCTGTTCACGATCGATTCGGCCGATCTCGCGCAGGCCAACAGCG
ACGCCGCCAAGGCCCGCGCGGCCATGACAATGGCACGCCGCAACCTGGATCGTCAGCGGGAACTGGACAAGTCAGAGATC
GCCGCAAAGCGCGACTTCGAGCAGGCCCAGAGCGACTATGACCAGGCCGCCAGCGAATCCCAGCGCGCCGATGCGCGTCT
GGCGCAACTTGGCGCCAAGGGTGGCGGCACGTTGCAGGCGGGCGGCGGCCACATCCTGGCCGTGCGATCGCCGATCAACG
GGCGCGTGGTCGATCTCAATGCCGCGACCGGCGCGTATTGGAACGACACCACGGCCTCCCTGATGACCGTTGCCGATCTC
TCGCACGTGTTTGTCACGGCCAACGCGCAGGAAAAGGATCTGGGCCACGTGTATGTCGGCCAGTCGGCCACCGTCAAGTT
CGACGCCTATGACGATCCGCAACCGGGCAAGGTGCGCTATGTCGGGCAGATCCTTGATGCGGACACGCGCACGACCAAGG
TGCGCATGGTGTTCGACAACCCCGACGGACGGCTCCGTCCCGGCATGTTCGCGCAGGCTACCTTCCTGTCCCAGCCGCAT
GAAGGCATCGTCGTGCCGATGTCGGCCATCGTGCAGAGCGGCTTCTACACCCGCGCATTCGTCGAAGTGGCGCCGTGGCA
GTTCGAGCCCCGTGTGATCAAGCTCGGCGCGCAAATCGGCGACCGTATGGAAGTGAAGTCGGGCCTGTCCGCGGGAGACC
GCGTGGTGGTGAAGGAAGGGGTGCTGCTGAATGATTGA

Upstream 100 bases:

>100_bases
AGAATTCAGAGTGCACGGGGGCTTGCGTGCGTACCATTGGCGGCAAATTCCCGCATGTGGCGATTCCATATCTTTTGAAA
CATCAGCATCGGAGTGAATG

Downstream 100 bases:

>100_bases
GCGCCTTGTTACCCTGTGCTTCAACCGGCGCGGCATCGTCGCGCTGGTGTTCGCGATGGTCGCGCTGTACGGCTGGTACG
CGTGGAAGCAGCTGCCCCTG

Product: membrane fusion protein (MFP-RND) heavy metal cation tricomponent efflux HmxB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKNKPAFPGRVVYWLAAAVILGLGGAGVWTMRAKAEQKRADPPVALRHEGERLVVPAESPLRRTLAVAPATRETVAAPFN
LPAMIEADPAKLVKVLPPLAGRIVSLNKQLGDEVKAGDVLFTIDSADLAQANSDAAKARAAMTMARRNLDRQRELDKSEI
AAKRDFEQAQSDYDQAASESQRADARLAQLGAKGGGTLQAGGGHILAVRSPINGRVVDLNAATGAYWNDTTASLMTVADL
SHVFVTANAQEKDLGHVYVGQSATVKFDAYDDPQPGKVRYVGQILDADTRTTKVRMVFDNPDGRLRPGMFAQATFLSQPH
EGIVVPMSAIVQSGFYTRAFVEVAPWQFEPRVIKLGAQIGDRMEVKSGLSAGDRVVVKEGVLLND

Sequences:

>Translated_385_residues
MKNKPAFPGRVVYWLAAAVILGLGGAGVWTMRAKAEQKRADPPVALRHEGERLVVPAESPLRRTLAVAPATRETVAAPFN
LPAMIEADPAKLVKVLPPLAGRIVSLNKQLGDEVKAGDVLFTIDSADLAQANSDAAKARAAMTMARRNLDRQRELDKSEI
AAKRDFEQAQSDYDQAASESQRADARLAQLGAKGGGTLQAGGGHILAVRSPINGRVVDLNAATGAYWNDTTASLMTVADL
SHVFVTANAQEKDLGHVYVGQSATVKFDAYDDPQPGKVRYVGQILDADTRTTKVRMVFDNPDGRLRPGMFAQATFLSQPH
EGIVVPMSAIVQSGFYTRAFVEVAPWQFEPRVIKLGAQIGDRMEVKSGLSAGDRVVVKEGVLLND
>Mature_385_residues
MKNKPAFPGRVVYWLAAAVILGLGGAGVWTMRAKAEQKRADPPVALRHEGERLVVPAESPLRRTLAVAPATRETVAAPFN
LPAMIEADPAKLVKVLPPLAGRIVSLNKQLGDEVKAGDVLFTIDSADLAQANSDAAKARAAMTMARRNLDRQRELDKSEI
AAKRDFEQAQSDYDQAASESQRADARLAQLGAKGGGTLQAGGGHILAVRSPINGRVVDLNAATGAYWNDTTASLMTVADL
SHVFVTANAQEKDLGHVYVGQSATVKFDAYDDPQPGKVRYVGQILDADTRTTKVRMVFDNPDGRLRPGMFAQATFLSQPH
EGIVVPMSAIVQSGFYTRAFVEVAPWQFEPRVIKLGAQIGDRMEVKSGLSAGDRVVVKEGVLLND

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Attached To The Inner Membrane By A Lipid Anchor [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=309, Percent_Identity=27.1844660194175, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082036, Length=327, Percent_Identity=26.605504587156, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786668, Length=313, Percent_Identity=28.7539936102236, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081796, Length=356, Percent_Identity=26.4044943820225, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789665, Length=311, Percent_Identity=27.9742765273312, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081951, Length=204, Percent_Identity=27.4509803921569, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41376; Mature: 41376

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKPAFPGRVVYWLAAAVILGLGGAGVWTMRAKAEQKRADPPVALRHEGERLVVPAESP
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCCEEEEECCCEEEECCCCC
LRRTLAVAPATRETVAAPFNLPAMIEADPAKLVKVLPPLAGRIVSLNKQLGDEVKAGDVL
HHHHEEECCCCHHHEECCCCCCEEEECCHHHHHHHCCCHHHHEEHHHHHHCCCCCCCCEE
FTIDSADLAQANSDAAKARAAMTMARRNLDRQRELDKSEIAAKRDFEQAQSDYDQAASES
EEECCHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QRADARLAQLGAKGGGTLQAGGGHILAVRSPINGRVVDLNAATGAYWNDTTASLMTVADL
HHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHCCC
SHVFVTANAQEKDLGHVYVGQSATVKFDAYDDPQPGKVRYVGQILDADTRTTKVRMVFDN
CEEEEECCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEEEEC
PDGRLRPGMFAQATFLSQPHEGIVVPMSAIVQSGFYTRAFVEVAPWQFEPRVIKLGAQIG
CCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHCCCCEEEEEEECCCCCCCEEEEECHHCC
DRMEVKSGLSAGDRVVVKEGVLLND
CHHHHHCCCCCCCEEEEECCEEECC
>Mature Secondary Structure
MKNKPAFPGRVVYWLAAAVILGLGGAGVWTMRAKAEQKRADPPVALRHEGERLVVPAESP
CCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCCCCEEEEECCCEEEECCCCC
LRRTLAVAPATRETVAAPFNLPAMIEADPAKLVKVLPPLAGRIVSLNKQLGDEVKAGDVL
HHHHEEECCCCHHHEECCCCCCEEEECCHHHHHHHCCCHHHHEEHHHHHHCCCCCCCCEE
FTIDSADLAQANSDAAKARAAMTMARRNLDRQRELDKSEIAAKRDFEQAQSDYDQAASES
EEECCHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QRADARLAQLGAKGGGTLQAGGGHILAVRSPINGRVVDLNAATGAYWNDTTASLMTVADL
HHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHCCC
SHVFVTANAQEKDLGHVYVGQSATVKFDAYDDPQPGKVRYVGQILDADTRTTKVRMVFDN
CEEEEECCCCCCCCCEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEEEEC
PDGRLRPGMFAQATFLSQPHEGIVVPMSAIVQSGFYTRAFVEVAPWQFEPRVIKLGAQIG
CCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHCCCCEEEEEEECCCCCCCEEEEECHHCC
DRMEVKSGLSAGDRVVVKEGVLLND
CHHHHHCCCCCCCEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]