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The map label for this gene is zneC [H]

Identifier: 94314247

GI number: 94314247

Start: 2037435

End: 2038970

Strand: Reverse

Name: zneC [H]

Synonym: Rmet_5328

Alternate gene names: 94314247

Gene position: 2038970-2037435 (Counterclockwise)

Preceding gene: 94314248

Following gene: 94314243

Centisome position: 79.03

GC content: 69.66

Gene sequence:

>1536_bases
ATGGCTAAAATCACTTCGATCAGCGTGGCGACGCTGCTGGCGCTGGCCACCGCGGGTTGCGCGGTGGGCCCGGACTTCCA
GCGCCCGGCCGCGCCCGAGACCGATCGCTACACGGCGACGACATTGCCCGCGGAGACCGCATCGGCATCCACGCCGGATG
GGCAGGCGCAGCGCTTCGCGCCGGGGCAGGATGTGCCGGCGCAGTGGTGGACGATGTTTGGCTCCAGCGAACTCAACGCG
CTGGTGGACGCCGCATTGCGCGCGAACCCGGACCTGCAGGCGATGGAGGCCGCGCTGCGCGTGGCCCAGGAGAACGTAGC
GGCGCAGCGTGGCGCGTTCTTCCCGAGCGTTGACGCGTCGTACACGCCGTCGCGCCAGAAGATCGCCCAGATCATCGCGA
GCCCGCTGTCCGACAACAGCGATCTGTTCACGCTGCACACGGCGCAGTTGAGTGTCTCGTACGTGCCCGACGTCTTCGGC
GGCACGCGCCGGCAGGTGGAGTCAGGCGTGGCGCAGGCCGACGTTGCCCGGTTCCAGTGGCAGGCCGCGTACCTGACGCT
GACGTCGAACGTCGTCAATGCGGCAATCCAGGAAGCCTCGCTGCGCGCCCAGATCCGCGCGACGCAAGACGTCATCGCGC
TATCCACGCGCCAGCTCGAGGCCGTGCGCAAGCAGCAGCGCCTAGGGCAGGTCGGCGCCGCCGAGGTCGCCGCACAGGAA
GCCACGCTGGCGCAGGCGGAAGCGGCATTGCCGCCGCTGGACCGGCAACTCGCCCAGCAGCGCAACCTGCTCGCGGCATT
GACGGGCCGGTTGCCGAGCGACGCCGTGCCGCAGCAGTTCGAGTTCGAAGCGCTGACGCTGCCCGGGACATTGCCGCTGA
GCCTGCCGTCGAGGCTGGTTGGCCAGCGCCCCGATATTCGCGCGGCGGAAGCGCAGATGCACGCGGCCAGCGCCCAGATC
GGCGTGGCCACTGCGGCCCGCCTGCCGAATATCACGCTGACCGCCGCGCTCGGTAGCTCGTCGCAGGCGATTGGGGATCT
CTTCACGGCCGGCACCGGCTTCTGGGGAATCTCCGCGGGCCTGATGCAGCCGATCTTCAAGGGCGGGATGCTGCTGCACC
AGCAGCGCGCGGCCGAGGCGGCCTACAAGCAGGCATCTGCGCAGTACCGCAGCACGGTGCTGACCGCCTATCAGGGGGTG
GCCGACGCGCTGCATGCGATCGAGGCCGATGCGCGGGGACTGCGCACTGCATCGGATGCCGAGCGTGCCGCGTACCGGAG
CTTCAACATCGCCCAGGCGCAGTGGAAGGCCGGACAGATCGGTTACCCGGCCGTGATGCTGGCCGAGCAGACGTATCGGC
AGTCGGCGATCACGCTGGTACAGGCGCGCGCGAGCCGTTACTCCGATACCGTGGGCCTGATGCAGGCGCTGGGCGGAAGC
TGGGAGGATGAACCGGGCAAGACGCCGGAGAACCAGTCGGACAGCAAGCCGGCCGCAGCGCCGGAGGCCGTCGGCAAGGC
TCAGGGTAACGGCTGA

Upstream 100 bases:

>100_bases
TGTTGCCCGCGTTGTATTACCTGATCGAGACACGCGCGGCGAAGCAGGTGCGCGAGGAGCCGCCGGTACAGTTCGGCCCC
ACCAGTGAAGGGGATTTGTA

Downstream 100 bases:

>100_bases
TCCGTGGTGGCAGCCGCGTGCTGCCGACGGACGCCGCTCTCGTTGGTACCGTAGTTGCCACCACAGTTGTCACCACACGG
CACGCGCACCGTCACGCGCG

Product: heavy metal cation tricomponent efflux outer membrane porin ZneC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MAKITSISVATLLALATAGCAVGPDFQRPAAPETDRYTATTLPAETASASTPDGQAQRFAPGQDVPAQWWTMFGSSELNA
LVDAALRANPDLQAMEAALRVAQENVAAQRGAFFPSVDASYTPSRQKIAQIIASPLSDNSDLFTLHTAQLSVSYVPDVFG
GTRRQVESGVAQADVARFQWQAAYLTLTSNVVNAAIQEASLRAQIRATQDVIALSTRQLEAVRKQQRLGQVGAAEVAAQE
ATLAQAEAALPPLDRQLAQQRNLLAALTGRLPSDAVPQQFEFEALTLPGTLPLSLPSRLVGQRPDIRAAEAQMHAASAQI
GVATAARLPNITLTAALGSSSQAIGDLFTAGTGFWGISAGLMQPIFKGGMLLHQQRAAEAAYKQASAQYRSTVLTAYQGV
ADALHAIEADARGLRTASDAERAAYRSFNIAQAQWKAGQIGYPAVMLAEQTYRQSAITLVQARASRYSDTVGLMQALGGS
WEDEPGKTPENQSDSKPAAAPEAVGKAQGNG

Sequences:

>Translated_511_residues
MAKITSISVATLLALATAGCAVGPDFQRPAAPETDRYTATTLPAETASASTPDGQAQRFAPGQDVPAQWWTMFGSSELNA
LVDAALRANPDLQAMEAALRVAQENVAAQRGAFFPSVDASYTPSRQKIAQIIASPLSDNSDLFTLHTAQLSVSYVPDVFG
GTRRQVESGVAQADVARFQWQAAYLTLTSNVVNAAIQEASLRAQIRATQDVIALSTRQLEAVRKQQRLGQVGAAEVAAQE
ATLAQAEAALPPLDRQLAQQRNLLAALTGRLPSDAVPQQFEFEALTLPGTLPLSLPSRLVGQRPDIRAAEAQMHAASAQI
GVATAARLPNITLTAALGSSSQAIGDLFTAGTGFWGISAGLMQPIFKGGMLLHQQRAAEAAYKQASAQYRSTVLTAYQGV
ADALHAIEADARGLRTASDAERAAYRSFNIAQAQWKAGQIGYPAVMLAEQTYRQSAITLVQARASRYSDTVGLMQALGGS
WEDEPGKTPENQSDSKPAAAPEAVGKAQGNG
>Mature_510_residues
AKITSISVATLLALATAGCAVGPDFQRPAAPETDRYTATTLPAETASASTPDGQAQRFAPGQDVPAQWWTMFGSSELNAL
VDAALRANPDLQAMEAALRVAQENVAAQRGAFFPSVDASYTPSRQKIAQIIASPLSDNSDLFTLHTAQLSVSYVPDVFGG
TRRQVESGVAQADVARFQWQAAYLTLTSNVVNAAIQEASLRAQIRATQDVIALSTRQLEAVRKQQRLGQVGAAEVAAQEA
TLAQAEAALPPLDRQLAQQRNLLAALTGRLPSDAVPQQFEFEALTLPGTLPLSLPSRLVGQRPDIRAAEAQMHAASAQIG
VATAARLPNITLTAALGSSSQAIGDLFTAGTGFWGISAGLMQPIFKGGMLLHQQRAAEAAYKQASAQYRSTVLTAYQGVA
DALHAIEADARGLRTASDAERAAYRSFNIAQAQWKAGQIGYPAVMLAEQTYRQSAITLVQARASRYSDTVGLMQALGGSW
EDEPGKTPENQSDSKPAAAPEAVGKAQGNG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=24.6835443037975, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53839; Mature: 53708

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITSISVATLLALATAGCAVGPDFQRPAAPETDRYTATTLPAETASASTPDGQAQRFA
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
PGQDVPAQWWTMFGSSELNALVDAALRANPDLQAMEAALRVAQENVAAQRGAFFPSVDAS
CCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YTPSRQKIAQIIASPLSDNSDLFTLHTAQLSVSYVPDVFGGTRRQVESGVAQADVARFQW
CCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCHHCCCHHHHHHHCHHHHHHHHHHH
QAAYLTLTSNVVNAAIQEASLRAQIRATQDVIALSTRQLEAVRKQQRLGQVGAAEVAAQE
HHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ATLAQAEAALPPLDRQLAQQRNLLAALTGRLPSDAVPQQFEFEALTLPGTLPLSLPSRLV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHH
GQRPDIRAAEAQMHAASAQIGVATAARLPNITLTAALGSSSQAIGDLFTAGTGFWGISAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHH
LMQPIFKGGMLLHQQRAAEAAYKQASAQYRSTVLTAYQGVADALHAIEADARGLRTASDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ERAAYRSFNIAQAQWKAGQIGYPAVMLAEQTYRQSAITLVQARASRYSDTVGLMQALGGS
HHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
WEDEPGKTPENQSDSKPAAAPEAVGKAQGNG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
AKITSISVATLLALATAGCAVGPDFQRPAAPETDRYTATTLPAETASASTPDGQAQRFA
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
PGQDVPAQWWTMFGSSELNALVDAALRANPDLQAMEAALRVAQENVAAQRGAFFPSVDAS
CCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
YTPSRQKIAQIIASPLSDNSDLFTLHTAQLSVSYVPDVFGGTRRQVESGVAQADVARFQW
CCCCHHHHHHHHHHCCCCCCCEEEEEEEEEEEECCCHHCCCHHHHHHHCHHHHHHHHHHH
QAAYLTLTSNVVNAAIQEASLRAQIRATQDVIALSTRQLEAVRKQQRLGQVGAAEVAAQE
HHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ATLAQAEAALPPLDRQLAQQRNLLAALTGRLPSDAVPQQFEFEALTLPGTLPLSLPSRLV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHH
GQRPDIRAAEAQMHAASAQIGVATAARLPNITLTAALGSSSQAIGDLFTAGTGFWGISAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHH
LMQPIFKGGMLLHQQRAAEAAYKQASAQYRSTVLTAYQGVADALHAIEADARGLRTASDA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
ERAAYRSFNIAQAQWKAGQIGYPAVMLAEQTYRQSAITLVQARASRYSDTVGLMQALGGS
HHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
WEDEPGKTPENQSDSKPAAAPEAVGKAQGNG
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]