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The map label for this gene is acnB [H]

Identifier: 94314215

GI number: 94314215

Start: 1997505

End: 2000096

Strand: Reverse

Name: acnB [H]

Synonym: Rmet_5296

Alternate gene names: 94314215

Gene position: 2000096-1997505 (Counterclockwise)

Preceding gene: 94314225

Following gene: 94314213

Centisome position: 77.52

GC content: 63.58

Gene sequence:

>2592_bases
ATGCTTGATACCTATCGCGCCCATGTCGCCGAACGCGCTGCGCTTGGCATTCCCCCTCTGCCTCTGACCGCCAAGCAGAC
CGCCGACCTGATCGAACTGATCAAGAACCCGCCGGCGGGCGAGGAGCAGGCGCTGCTGGACCTGATTACCTATCGCGTGC
CTGCAGGCGTGGATGACGCCGCCAAGGTCAAGGCCTCGTACCTGGCCGCCGTGGCCCTGGGTTCGGAAAAGACCCCGCTG
ATCTCGCGCGCCCGCGCTACCGAACTGCTCGGCACGATGCTGGGCGGCTATAACATCTCGCCGCTGATCGAGCTGCTGGA
CGATGCCGAAGTGGGCACCGTGGCTGCCGAGGCGCTGAAGAAGACGCTGCTGATGTTCGACGCCTTCCACGACGTGAAGG
AAAAGGCGGACAAGGGCAACGCCAATGCCAAGGCCGTGCTGCAAAGCTGGGCCGACGCCGAATGGTTCACGAGCCGTCCG
GAACTGCCGCAATCGCTGACGATCACCGTGTTCAAGGTGCCGGGCGAGACCAACACCGATGACCTGTCGCCGGCCCCTGA
CGCCACCACGCGCCCGGACATCCCAATGCACGCGCTGGCCATGCTGAAGAACAAGCGCGAAGGCGCCGCGTTCCAGCCGG
AAGAAGATGGCAAGCGTGGCCCGGTCAAGTTCATCGAGTCGCTGAAGGACAAGGGCCACCTGGTGGCCTACGTTGGCGAT
GTGGTCGGTACCGGCTCCAGCCGCAAGTCGGCCACCAACTCGGTGCTGTGGTTCACTGGCGAAGACATCCCGTTCGTGCC
GAACAAGCGTTTCGGTGGCGTGTGCCTGGGCAGCAAGATTGCTCCGATCTTCTACAACACGATGGAAGACGCCGGCGCGC
TGCCGATCGAACTCGACGTGTCGAAGATGGAAATGGGCGACGTGGTGGAACTGCGTCCCTACGAAGGCAAGGCCCTGAAG
AACGGCGAAGTCATCGCTGAATTCAAGGTGAAGTCGGACGTGCTGTTCGACGAAGTGCGCGCCGGTGGCCGCATTCCGCT
GATCATCGGCCGTGGCCTGACCGCCAAGGCCCGCGAGTCGCTGGGCCTGCCCCCGTCGACGCTGTTCCGCCTGCCGCAGA
ACCCGGTCGACACCGGCAAGGGTTTCACGCTGGCCCAGAAGATGGTCGGCCGCGCATGCGGCCTGGCCGAAGGCAAGGGC
ATCCGCCCGGGCACGTACTGCGAACCGAAGATGACCTCGGTGGGCTCGCAGGACACCACTGGCCCGATGACCCGTGACGA
GCTGAAGGATCTGGCTTGCCTGGGCTTCTCGGCCGACCTCGTGATGCAGTCGTTCTGCCACACCGCCGCTTATCCGAAGC
CGGTGGACGTGAAGACGCACCACACGCTGCCGCAGTTCATCAGCACCCGTGGCGGTATCTCGCTGCGTCCGGGCGACGGC
GTGATTCACTCGTGGCTGAACCGCATGCTGCTGCCCGACACCGTGGGCACCGGCGGCGACTCGCACACCCGCTTCCCGAT
CGGTATCAGCTTCCCGGCCGGTTCGGGACTGGTTGCCTTTGCCGCCGCCACCGGCGTAATGCCGCTGGATATGCCGGAAT
CGGTGCTGGTCCGCTTCAAGGGCAAGATGCAGCCTGGCGTGACGCTGCGTGACCTGGTCAATGCCATTCCGCTGTACGCG
ATCAAGCAGGGTCTGCTGACCGTGGCCAAGCAAGGCAAGCAGAACATCTTCTCGGGCCGTATCCTCGAAATCGAAGGTCT
GCCCGACCTGAAGGTGGAACAGGCCTTTGAACTGTCCGACGCCTCCGCAGAGCGTTCGGCCGCCGGTTGCTCGGTGCGCC
TGAACAAGGAACCGATCATCGAATACATCAACAGCAACATCACGCTGCTGAAGTGGATGATCGCCGAAGGCTACCAGGAC
CCGCGCAGCCTGCAGCGCCGTATCAAGGCCATGGAAGCATGGCTGGCCGATCCGAAGCTGCTGGAGCCGGACACCGACGC
CGAGTACGCCGCCGTGATCGAAATCGATCTGGCCGACGTGCACGAGCCGATCGTGGCCTGCCCGAACGACCCGGACGACG
TGAAGACGCTGTCCGACGTGGCCGGCGCCAAGATCGACGAAGTGTTCATCGGTTCGTGCATGACCAACATCGGCCACTTC
CGTGCCGCTTCGAAGCTGCTGGAAGGCAAGCGCGACATCCCCGTGAAGCTGTGGGTCGCCCCGCCGACCAAGATGGACCA
GAAGCAGCTGACCGAGGAAGGCCACTATGGCGTGTTCGGCACCGCTGGCGCGCGTACTGAAATGCCGGGCTGCTCGCTGT
GCATGGGTAACCAGGCACAGGTGCGCGAAGGCGCGACGGTGATGTCGACCTCGACGCGTAACTTCCCGAACCGTCTGGGC
AAGAACACGAACGTGTATCTCGGCTCCGCCGAACTGGCTGCCATCTGCTCGCGCCTGGGTCGTATCCCGAACAAGGAAGA
GTACATGGCCGACATGGGCGTGCTGAACGCCAACGGCGACAAGATCTACAAGTACATGAACTTCGACCAGATCGAAGACT
TCAAGGAAGTCGCGGACACGGTGTCGATCTAA

Upstream 100 bases:

>100_bases
GCGGGGACTTCGGTCTACAATCACGCGCAACTGGCCGCCTTGCGCACCAGTCCTCACAGAGACGGCAGTACCGTTACAAC
CCTATTACCAGCAGGTTCCT

Downstream 100 bases:

>100_bases
GTGTCGATGTTCCTCGGCGGCGACGCCGAGGAGCGAACTGAAGTCCGATGAACCCCGCGTGCGCGTGAAGCGCACGCGGG
GTTTTTTCATGGGTGATTGC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MLDTYRAHVAERAALGIPPLPLTAKQTADLIELIKNPPAGEEQALLDLITYRVPAGVDDAAKVKASYLAAVALGSEKTPL
ISRARATELLGTMLGGYNISPLIELLDDAEVGTVAAEALKKTLLMFDAFHDVKEKADKGNANAKAVLQSWADAEWFTSRP
ELPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKDKGHLVAYVGD
VVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFYNTMEDAGALPIELDVSKMEMGDVVELRPYEGKALK
NGEVIAEFKVKSDVLFDEVRAGGRIPLIIGRGLTAKARESLGLPPSTLFRLPQNPVDTGKGFTLAQKMVGRACGLAEGKG
IRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPQFISTRGGISLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYA
IKQGLLTVAKQGKQNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCSVRLNKEPIIEYINSNITLLKWMIAEGYQD
PRSLQRRIKAMEAWLADPKLLEPDTDAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHF
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNTNVYLGSAELAAICSRLGRIPNKEEYMADMGVLNANGDKIYKYMNFDQIEDFKEVADTVSI

Sequences:

>Translated_863_residues
MLDTYRAHVAERAALGIPPLPLTAKQTADLIELIKNPPAGEEQALLDLITYRVPAGVDDAAKVKASYLAAVALGSEKTPL
ISRARATELLGTMLGGYNISPLIELLDDAEVGTVAAEALKKTLLMFDAFHDVKEKADKGNANAKAVLQSWADAEWFTSRP
ELPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKDKGHLVAYVGD
VVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFYNTMEDAGALPIELDVSKMEMGDVVELRPYEGKALK
NGEVIAEFKVKSDVLFDEVRAGGRIPLIIGRGLTAKARESLGLPPSTLFRLPQNPVDTGKGFTLAQKMVGRACGLAEGKG
IRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPQFISTRGGISLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYA
IKQGLLTVAKQGKQNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCSVRLNKEPIIEYINSNITLLKWMIAEGYQD
PRSLQRRIKAMEAWLADPKLLEPDTDAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHF
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNTNVYLGSAELAAICSRLGRIPNKEEYMADMGVLNANGDKIYKYMNFDQIEDFKEVADTVSI
>Mature_863_residues
MLDTYRAHVAERAALGIPPLPLTAKQTADLIELIKNPPAGEEQALLDLITYRVPAGVDDAAKVKASYLAAVALGSEKTPL
ISRARATELLGTMLGGYNISPLIELLDDAEVGTVAAEALKKTLLMFDAFHDVKEKADKGNANAKAVLQSWADAEWFTSRP
ELPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKDKGHLVAYVGD
VVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFYNTMEDAGALPIELDVSKMEMGDVVELRPYEGKALK
NGEVIAEFKVKSDVLFDEVRAGGRIPLIIGRGLTAKARESLGLPPSTLFRLPQNPVDTGKGFTLAQKMVGRACGLAEGKG
IRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPQFISTRGGISLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYA
IKQGLLTVAKQGKQNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCSVRLNKEPIIEYINSNITLLKWMIAEGYQD
PRSLQRRIKAMEAWLADPKLLEPDTDAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHF
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNTNVYLGSAELAAICSRLGRIPNKEEYMADMGVLNANGDKIYKYMNFDQIEDFKEVADTVSI

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=864, Percent_Identity=73.1481481481482, Blast_Score=1270, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=375, Percent_Identity=24.8, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=358, Percent_Identity=25.4189944134078, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93214; Mature: 93214

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTYRAHVAERAALGIPPLPLTAKQTADLIELIKNPPAGEEQALLDLITYRVPAGVDDA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
AKVKASYLAAVALGSEKTPLISRARATELLGTMLGGYNISPLIELLDDAEVGTVAAEALK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDAFHDVKEKADKGNANAKAVLQSWADAEWFTSRPELPQSLTITVFKVPGETNTD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKDKGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECH
VVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFYNTMEDAGALPIELDV
HHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEEH
SKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIIGRGLTAKARES
HHHCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLPPSTLFRLPQNPVDTGKGFTLAQKMVGRACGLAEGKGIRPGTYCEPKMTSVGSQDTT
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPQFISTRGGISLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCH
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCC
GKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKQNIFSGRILEIEGLPDLKVEQAFELSD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAGCSVRLNKEPIIEYINSNITLLKWMIAEGYQDPRSLQRRIKAMEAWLADPKL
CCHHHCCCCCEEEECCHHHHHHHCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LEPDTDAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHH
VREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGRIPNKEEYMADMGVLNANGD
HHCCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHCEEECCCC
KIYKYMNFDQIEDFKEVADTVSI
CEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDTYRAHVAERAALGIPPLPLTAKQTADLIELIKNPPAGEEQALLDLITYRVPAGVDDA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHH
AKVKASYLAAVALGSEKTPLISRARATELLGTMLGGYNISPLIELLDDAEVGTVAAEALK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDAFHDVKEKADKGNANAKAVLQSWADAEWFTSRPELPQSLTITVFKVPGETNTD
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKDKGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECH
VVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSKIAPIFYNTMEDAGALPIELDV
HHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEEH
SKMEMGDVVELRPYEGKALKNGEVIAEFKVKSDVLFDEVRAGGRIPLIIGRGLTAKARES
HHHCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LGLPPSTLFRLPQNPVDTGKGFTLAQKMVGRACGLAEGKGIRPGTYCEPKMTSVGSQDTT
CCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPQFISTRGGISLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCH
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCC
GKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKQNIFSGRILEIEGLPDLKVEQAFELSD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAGCSVRLNKEPIIEYINSNITLLKWMIAEGYQDPRSLQRRIKAMEAWLADPKL
CCHHHCCCCCEEEECCHHHHHHHCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LEPDTDAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHH
VREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGRIPNKEEYMADMGVLNANGD
HHCCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHCEEECCCC
KIYKYMNFDQIEDFKEVADTVSI
CEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]