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The map label for this gene is hipO [H]

Identifier: 94314141

GI number: 94314141

Start: 1916120

End: 1917316

Strand: Direct

Name: hipO [H]

Synonym: Rmet_5222

Alternate gene names: 94314141

Gene position: 1916120-1917316 (Clockwise)

Preceding gene: 94314140

Following gene: 94314142

Centisome position: 74.27

GC content: 63.66

Gene sequence:

>1197_bases
TTGACGATGGCCATCGATTCCAAGGCGAGTACAGCGCTTCCCGACCTGCTCCCCCTGCACGACGAGCTTGCCGCGCTGCG
ACAGCACATCCATCGGCATCCGGAGCTGGCCTTTGAGGAGCACGACACTGCGGATCTGGTTGCCGGGAAGTTGCAGGAAT
GGGGATACCACGTAACGCGCGGCATCGGAAAGACGGGGCTGGTTGGCCAGCTGCGCGTGGGAAACGGTGACAGGTGTCTT
GGCCTGCGCGCCGACATGGATGCCCTGCCTATCCAGGAAGACACCGGGCTTCCCTACGCCAGCATCCGGCCCGGCCTCAT
GCATGCCTGTGGACACGATGGCCACACCGCGATGCTGCTTGGTGCGGCAAAGCACCTTGCGGCCACACGGAACTTCTCCG
GTACGTTGAACCTGATCTTTCAGCCTGCAGAGGAGCGCGGGTTCGACAGCGGCGCCAAGGCCATGGTGGCGGATGGTTTG
TTCGACCGCTTTCCTTGCGACATGGTGTTCGCCATGCACAACCATCCCGGTGCACCCCAAGGCCGGTTCCTGATGCGTCC
GGGACCGTTTCTCGCCGCGGGCGATCGGGTGTTCATCAAGGTCATCGGCCTGGGCGGCCACGCCGCGCGCCCTCATCTTG
CGGTCGATCCACTTGTGGCAGCGGCTGCCATCGTGATGGCACTCCAGACCGTAGTCTCCCGCAACGTCCCCCCCAACGAG
TCGGCAGTACTCACGGTTGGCCGGCTACGTGCGGGAGACGCACTGAACGTTATTCCGGCCGAAGCCGAAATCGGCATCAG
CGTGCGCTCGTTCAGTCCGGAGGTGCGCGCACTATTGAGGGATCGCATCACCACGCTGGTCCACATGACTGCCGAATCGT
ATGGCGCGAAAGCTGAGATCCGCTATGTGGAAGGCTACCCGGTTGTCGACAACGCCGCCGACGCGGTTGCCCTGGCAGCG
GAAGTTGCCACGGCGCTTGTTGGCCCGGAGGACGTGGACCGCGACTATCCGAGGATGATGGGTAGCGAGGACTTCGCCTA
CATGCTGCAGGAATGCCCGGGGGCGCTCGTCCGCATTGGTAACGGCCCATCTGACGGCGGGCGCGGCTTGCACAATCCGC
GCTACGACTTCAATGACCGCAACCTGGCGTTGGGTGCAGCGTATTGGTGCCGACTGACAGAGACGTTCCTGGCCTGA

Upstream 100 bases:

>100_bases
TCGTGCCCGAGGACATTACGCCGGAAGCCTATACCAGGGAAACGGAGCAGCGCATCAAGTTCTACCAGCGCATCGCCAAA
GACAACCACATCACTTTGGA

Downstream 100 bases:

>100_bases
GCCCAGCCTGGGAGACGAAAGCAGTGCCTGCGGCGGCGAAGAGGGGCAATCGAACAACCCGCATGGGTCGGGCAGAGGCG
GCCTCGGCTTCTTAGCGGCC

Product: Hippurate hydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTMAIDSKASTALPDLLPLHDELAALRQHIHRHPELAFEEHDTADLVAGKLQEWGYHVTRGIGKTGLVGQLRVGNGDRCL
GLRADMDALPIQEDTGLPYASIRPGLMHACGHDGHTAMLLGAAKHLAATRNFSGTLNLIFQPAEERGFDSGAKAMVADGL
FDRFPCDMVFAMHNHPGAPQGRFLMRPGPFLAAGDRVFIKVIGLGGHAARPHLAVDPLVAAAAIVMALQTVVSRNVPPNE
SAVLTVGRLRAGDALNVIPAEAEIGISVRSFSPEVRALLRDRITTLVHMTAESYGAKAEIRYVEGYPVVDNAADAVALAA
EVATALVGPEDVDRDYPRMMGSEDFAYMLQECPGALVRIGNGPSDGGRGLHNPRYDFNDRNLALGAAYWCRLTETFLA

Sequences:

>Translated_398_residues
MTMAIDSKASTALPDLLPLHDELAALRQHIHRHPELAFEEHDTADLVAGKLQEWGYHVTRGIGKTGLVGQLRVGNGDRCL
GLRADMDALPIQEDTGLPYASIRPGLMHACGHDGHTAMLLGAAKHLAATRNFSGTLNLIFQPAEERGFDSGAKAMVADGL
FDRFPCDMVFAMHNHPGAPQGRFLMRPGPFLAAGDRVFIKVIGLGGHAARPHLAVDPLVAAAAIVMALQTVVSRNVPPNE
SAVLTVGRLRAGDALNVIPAEAEIGISVRSFSPEVRALLRDRITTLVHMTAESYGAKAEIRYVEGYPVVDNAADAVALAA
EVATALVGPEDVDRDYPRMMGSEDFAYMLQECPGALVRIGNGPSDGGRGLHNPRYDFNDRNLALGAAYWCRLTETFLA
>Mature_397_residues
TMAIDSKASTALPDLLPLHDELAALRQHIHRHPELAFEEHDTADLVAGKLQEWGYHVTRGIGKTGLVGQLRVGNGDRCLG
LRADMDALPIQEDTGLPYASIRPGLMHACGHDGHTAMLLGAAKHLAATRNFSGTLNLIFQPAEERGFDSGAKAMVADGLF
DRFPCDMVFAMHNHPGAPQGRFLMRPGPFLAAGDRVFIKVIGLGGHAARPHLAVDPLVAAAAIVMALQTVVSRNVPPNES
AVLTVGRLRAGDALNVIPAEAEIGISVRSFSPEVRALLRDRITTLVHMTAESYGAKAEIRYVEGYPVVDNAADAVALAAE
VATALVGPEDVDRDYPRMMGSEDFAYMLQECPGALVRIGNGPSDGGRGLHNPRYDFNDRNLALGAAYWCRLTETFLA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=427, Percent_Identity=28.8056206088993, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 42703; Mature: 42572

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMAIDSKASTALPDLLPLHDELAALRQHIHRHPELAFEEHDTADLVAGKLQEWGYHVTR
CCEEECCCCCCCCCCHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHH
GIGKTGLVGQLRVGNGDRCLGLRADMDALPIQEDTGLPYASIRPGLMHACGHDGHTAMLL
CCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHHCCCCCCEEHHH
GAAKHLAATRNFSGTLNLIFQPAEERGFDSGAKAMVADGLFDRFPCDMVFAMHNHPGAPQ
HHHHHHHHHCCCCCEEEEEEECCHHCCCCCCCHHHHHHCHHHHCCHHEEEEECCCCCCCC
GRFLMRPGPFLAAGDRVFIKVIGLGGHAARPHLAVDPLVAAAAIVMALQTVVSRNVPPNE
CCEEECCCCCEEECCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SAVLTVGRLRAGDALNVIPAEAEIGISVRSFSPEVRALLRDRITTLVHMTAESYGAKAEI
CEEEEEECCCCCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
RYVEGYPVVDNAADAVALAAEVATALVGPEDVDRDYPRMMGSEDFAYMLQECPGALVRIG
EEECCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCHHHHHHHHHCCCEEEEEC
NGPSDGGRGLHNPRYDFNDRNLALGAAYWCRLTETFLA
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMAIDSKASTALPDLLPLHDELAALRQHIHRHPELAFEEHDTADLVAGKLQEWGYHVTR
CEEECCCCCCCCCCHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHH
GIGKTGLVGQLRVGNGDRCLGLRADMDALPIQEDTGLPYASIRPGLMHACGHDGHTAMLL
CCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHHCCCCCCEEHHH
GAAKHLAATRNFSGTLNLIFQPAEERGFDSGAKAMVADGLFDRFPCDMVFAMHNHPGAPQ
HHHHHHHHHCCCCCEEEEEEECCHHCCCCCCCHHHHHHCHHHHCCHHEEEEECCCCCCCC
GRFLMRPGPFLAAGDRVFIKVIGLGGHAARPHLAVDPLVAAAAIVMALQTVVSRNVPPNE
CCEEECCCCCEEECCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
SAVLTVGRLRAGDALNVIPAEAEIGISVRSFSPEVRALLRDRITTLVHMTAESYGAKAEI
CEEEEEECCCCCCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
RYVEGYPVVDNAADAVALAAEVATALVGPEDVDRDYPRMMGSEDFAYMLQECPGALVRIG
EEECCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCHHHHHHHHHCCCEEEEEC
NGPSDGGRGLHNPRYDFNDRNLALGAAYWCRLTETFLA
CCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]