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The map label for this gene is gcvA [H]

Identifier: 94314139

GI number: 94314139

Start: 1914112

End: 1915038

Strand: Reverse

Name: gcvA [H]

Synonym: Rmet_5220

Alternate gene names: 94314139

Gene position: 1915038-1914112 (Counterclockwise)

Preceding gene: 291481561

Following gene: 94314136

Centisome position: 74.22

GC content: 62.03

Gene sequence:

>927_bases
ATGAAAATTCGCTCTCCTTCACTGGTGGAACTGCATGCCTTTCTGGCGGTGAGTCGCCTGCGCAGCTTCCGCCTGGCGGC
CGAGGAACTTTGCGTCACCCAGGCGGCTGTCAGCAGAGCCGTGCAACGTCTGGAGCAGCACCTGGGTGATTGCCAGTTGT
TCGAGCGATCATCGCAGGGTGTGGCGCTGACCGAAGAAGGCCGCCAGTTACGCAAGTTGACGGAGCGCCATGTGCTGGCC
CTCGAGGCTGCGGCGACCAGACTTCAGGGGCCACGCGCTGACGGGAAACTGCGGCTGAGCGTGATTCCCACGCTCGGTAC
TCAATGGCTGCTTCCCCGCCTCGCGCGCTTCAGGGCGGCGCACCCGGATGTGGACGTCGAAATGCGGCAATTTCGCCATG
ACGAAGACTTCAGCCGCGATGACATCGACATCTGGATTGAGGTGAAGCGTCCCAAGCGACGTTGGCCGGCCAGAATGCAG
GTGAAATACCTTCTCGGACGCGAACTCGTTCCGGTCTGCGCGCCATCGTTGCTTTCGCGCCTGAAGCGTCCCAGAGACTT
GCTTGAAGAGGCGCTGCTCCACCATACGAACTTCCCGGAAAACTGGGCGCTCTGGGCCAAGGTGGCCGGACTCGGGGTCG
CACCCAAGCTGGGCCCAGGCTACGACCTGAGCATCAATCTGATCTTTGCGGCGAAGGCCGGACTGGGTATCGCGCTAACG
CAACCCTGCCTGATCGAACGCGAGCTGGAAGCCGGTGAACTGGTGATGCCTTTTGATTTGCGAGTCGACACCGGGCGCGG
CTATTTCACCTGTGTCGACGCACAGCGGCCACGGAGCGCCGCGCAGAATGCATTTCTGGACTGGATTCTGGCGGAGGCTC
GCCAAAGCGAACCGGCTGGCGCTTTCGTATCGGGGCAACAGGGTTGA

Upstream 100 bases:

>100_bases
ATTGCCGGCGTGCGAGTTAGCGTTGTCCGAAGGTTAAGCAGACGCAGATCCGCAAGCAAACGACAAAACCATTGAGCACG
ATAACAAAATGTAATGACCC

Downstream 100 bases:

>100_bases
CGGGGGAGGGCAGTACGAGCTGGCGAATAGGCTGAACGAAGACGGGTTGATCGATCTTCCTGGACGATCTGAGCGGGCGC
CAATCGCCAGGGTCATGGTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKIRSPSLVELHAFLAVSRLRSFRLAAEELCVTQAAVSRAVQRLEQHLGDCQLFERSSQGVALTEEGRQLRKLTERHVLA
LEAAATRLQGPRADGKLRLSVIPTLGTQWLLPRLARFRAAHPDVDVEMRQFRHDEDFSRDDIDIWIEVKRPKRRWPARMQ
VKYLLGRELVPVCAPSLLSRLKRPRDLLEEALLHHTNFPENWALWAKVAGLGVAPKLGPGYDLSINLIFAAKAGLGIALT
QPCLIERELEAGELVMPFDLRVDTGRGYFTCVDAQRPRSAAQNAFLDWILAEARQSEPAGAFVSGQQG

Sequences:

>Translated_308_residues
MKIRSPSLVELHAFLAVSRLRSFRLAAEELCVTQAAVSRAVQRLEQHLGDCQLFERSSQGVALTEEGRQLRKLTERHVLA
LEAAATRLQGPRADGKLRLSVIPTLGTQWLLPRLARFRAAHPDVDVEMRQFRHDEDFSRDDIDIWIEVKRPKRRWPARMQ
VKYLLGRELVPVCAPSLLSRLKRPRDLLEEALLHHTNFPENWALWAKVAGLGVAPKLGPGYDLSINLIFAAKAGLGIALT
QPCLIERELEAGELVMPFDLRVDTGRGYFTCVDAQRPRSAAQNAFLDWILAEARQSEPAGAFVSGQQG
>Mature_308_residues
MKIRSPSLVELHAFLAVSRLRSFRLAAEELCVTQAAVSRAVQRLEQHLGDCQLFERSSQGVALTEEGRQLRKLTERHVLA
LEAAATRLQGPRADGKLRLSVIPTLGTQWLLPRLARFRAAHPDVDVEMRQFRHDEDFSRDDIDIWIEVKRPKRRWPARMQ
VKYLLGRELVPVCAPSLLSRLKRPRDLLEEALLHHTNFPENWALWAKVAGLGVAPKLGPGYDLSINLIFAAKAGLGIALT
QPCLIERELEAGELVMPFDLRVDTGRGYFTCVDAQRPRSAAQNAFLDWILAEARQSEPAGAFVSGQQG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=37.5838926174497, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=31.7073170731707, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1788706, Length=301, Percent_Identity=27.906976744186, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI145693193, Length=265, Percent_Identity=29.0566037735849, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=27, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=34.2657342657343, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=123, Percent_Identity=34.9593495934959, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=30.0813008130081, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34605; Mature: 34605

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSPSLVELHAFLAVSRLRSFRLAAEELCVTQAAVSRAVQRLEQHLGDCQLFERSSQG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
VALTEEGRQLRKLTERHVLALEAAATRLQGPRADGKLRLSVIPTLGTQWLLPRLARFRAA
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
HPDVDVEMRQFRHDEDFSRDDIDIWIEVKRPKRRWPARMQVKYLLGRELVPVCAPSLLSR
CCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
LKRPRDLLEEALLHHTNFPENWALWAKVAGLGVAPKLGPGYDLSINLIFAAKAGLGIALT
HHCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEC
QPCLIERELEAGELVMPFDLRVDTGRGYFTCVDAQRPRSAAQNAFLDWILAEARQSEPAG
CCHHHHCCCCCCCEEEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
AFVSGQQG
CCCCCCCC
>Mature Secondary Structure
MKIRSPSLVELHAFLAVSRLRSFRLAAEELCVTQAAVSRAVQRLEQHLGDCQLFERSSQG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
VALTEEGRQLRKLTERHVLALEAAATRLQGPRADGKLRLSVIPTLGTQWLLPRLARFRAA
EEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
HPDVDVEMRQFRHDEDFSRDDIDIWIEVKRPKRRWPARMQVKYLLGRELVPVCAPSLLSR
CCCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
LKRPRDLLEEALLHHTNFPENWALWAKVAGLGVAPKLGPGYDLSINLIFAAKAGLGIALT
HHCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCEEEC
QPCLIERELEAGELVMPFDLRVDTGRGYFTCVDAQRPRSAAQNAFLDWILAEARQSEPAG
CCHHHHCCCCCCCEEEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCC
AFVSGQQG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]