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The map label for this gene is lcfB [H]

Identifier: 94314077

GI number: 94314077

Start: 1856768

End: 1858351

Strand: Direct

Name: lcfB [H]

Synonym: Rmet_5158

Alternate gene names: 94314077

Gene position: 1856768-1858351 (Clockwise)

Preceding gene: 94314076

Following gene: 94314078

Centisome position: 71.97

GC content: 66.04

Gene sequence:

>1584_bases
ATGACAACACTGCACGACTGGGCAACCGTGGCGCCGCAAAAGCCAGCATTGATCCAGGCTGAATCGGGCCACGTCATCAC
GTATGGAGAGTTGGACGCGCGCGCGATCCGCGCCGCGCAATGGCTGGTGTCGCGTGGTCTGGAAGCCGGCGACTGCATCG
CCGTGCTGGTGGAGAATCACCCGGGCCTGATCGAGATCGCATCCGCGGCGCGGCGCGTCGGGCTGTACTACACCGCAGTC
AACACGCACCTGAGCCCGGCGGAAGCGGCCTACGTGCTGAAGGATTGCGGAGCGAAACTGCTGATCGCGTCGCCCGCCAT
GTTGCCGCTGGTCGAAGCAGTACGCGCGGCCGATGCCCACGCGCTCGATCTGCCTGTCTTTGTGCTGGCTGGCGCTTCGA
ACCTGCCGCCAGGCTTTGAAGCGTACGAAGCCGCAATCTCCGCGTTCGCCCCTGACGCCACCCTGCCCCCGCGTCCGGTC
GGCCGCGACATGCTCTATTCGTCCGGCACCACCGGCCACCCCAAGGGTATCCGCCGGCCGCTCACGCCCTACGAAGACCG
CGACAAACCTGAGCTTGAAATCATCGCCTGGCGCCGTGCATTCGGTTTCGACGAGAACACGGTCTACCTGTCGACCGCGC
CGTTCTATCACGCCGCGCCGCTGCGCTACATCATGCGTACGCTTGACGTCGGCGGCACATGCGTCGCCATGTCGAAATAC
GACGCCGAGGGCGCGCTGGCCGCAGTCGAGCGCTACCGCGTCACACATAGCCAGTGGGTGCCGACGATGTTTGTGCGCCT
GCTGGGCCTGCCCGACGAGGCACGCCGCCGTTATGACCTCTCGTCGATGAAGGTCGCCATCCACGCCGCCGCGCCATGCC
CGGTCCATGTCAAACAGGCCATGCTCGACTGGTGGGGCGACATCCTTTACGAGTACTACGCCGGCTCCGAAGGCGCCGGC
ACGACCTCCATCGGGCCACTGGAATGGCGCAAGTATCCGGGCTCGGTCGGACGCGCGTCGGCCGGCGTCATCCACATCGT
GGATAACGCCGGCAACGAGTTGCCGCCCAACGAAGTCGGCATGATCTACTTCTCGGGCGTGGCAACGTTCTCGTACCACA
ACGCCCCGGAGAAGACGCGATCCGCGTACAACGACAAGGGCTGGGCCACCTATGGAGACCTCGGCTACGTCAACGAGGAA
GGGTATCTGTTCCTGAGCGACCGGCGCGCGGACCTGATCCTGTCGGGCGGCGTGAACGTCTATCCGCAGGAAATCGAAAG
CGTGCTGATGCAGCACCCGTCGGTGGCGGACGTCGCCGTGATCGGCGTGCCCGATCCGGAGTTGGGCGAGGTACCGAAGG
CCGTGATCGAACTGCGTGACGGCAAGGCCGCCACGCCGGATCTGGCGCAGGACATCGTCGACTACTGCCAGGGCAGGCTC
GGTCGCCTGAAGCTGCCGCGCACGGTGGTCTTCGACGAACGCCTGCCACGCACCCCCACCGGCAAGCTGTTGCGACGGGA
ACTGAAGGACCGCCATCGCGAGCAACCGCACTCCGGGCATGCCGTGCGGACCGGCCGCGCCTGA

Upstream 100 bases:

>100_bases
CTCGGCCCGCGCCTGCTGACGCTCATGCGCAATGTGGAGGCATCGCTGGGCGCATAGCGTCGCAATAGAATACAAAACCA
ACAGGAGGAGACGACCTGTT

Downstream 100 bases:

>100_bases
CCGTACCGACTCAAGGCGGCCCGGCCGCCTGATGGTTGGCGGGTTGGGGGTGGTGGGAGCCCCAGCACATGGAAATGCGG
CTATGCTGGCGGCAAAACGT

Product: acyl-CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MTTLHDWATVAPQKPALIQAESGHVITYGELDARAIRAAQWLVSRGLEAGDCIAVLVENHPGLIEIASAARRVGLYYTAV
NTHLSPAEAAYVLKDCGAKLLIASPAMLPLVEAVRAADAHALDLPVFVLAGASNLPPGFEAYEAAISAFAPDATLPPRPV
GRDMLYSSGTTGHPKGIRRPLTPYEDRDKPELEIIAWRRAFGFDENTVYLSTAPFYHAAPLRYIMRTLDVGGTCVAMSKY
DAEGALAAVERYRVTHSQWVPTMFVRLLGLPDEARRRYDLSSMKVAIHAAAPCPVHVKQAMLDWWGDILYEYYAGSEGAG
TTSIGPLEWRKYPGSVGRASAGVIHIVDNAGNELPPNEVGMIYFSGVATFSYHNAPEKTRSAYNDKGWATYGDLGYVNEE
GYLFLSDRRADLILSGGVNVYPQEIESVLMQHPSVADVAVIGVPDPELGEVPKAVIELRDGKAATPDLAQDIVDYCQGRL
GRLKLPRTVVFDERLPRTPTGKLLRRELKDRHREQPHSGHAVRTGRA

Sequences:

>Translated_527_residues
MTTLHDWATVAPQKPALIQAESGHVITYGELDARAIRAAQWLVSRGLEAGDCIAVLVENHPGLIEIASAARRVGLYYTAV
NTHLSPAEAAYVLKDCGAKLLIASPAMLPLVEAVRAADAHALDLPVFVLAGASNLPPGFEAYEAAISAFAPDATLPPRPV
GRDMLYSSGTTGHPKGIRRPLTPYEDRDKPELEIIAWRRAFGFDENTVYLSTAPFYHAAPLRYIMRTLDVGGTCVAMSKY
DAEGALAAVERYRVTHSQWVPTMFVRLLGLPDEARRRYDLSSMKVAIHAAAPCPVHVKQAMLDWWGDILYEYYAGSEGAG
TTSIGPLEWRKYPGSVGRASAGVIHIVDNAGNELPPNEVGMIYFSGVATFSYHNAPEKTRSAYNDKGWATYGDLGYVNEE
GYLFLSDRRADLILSGGVNVYPQEIESVLMQHPSVADVAVIGVPDPELGEVPKAVIELRDGKAATPDLAQDIVDYCQGRL
GRLKLPRTVVFDERLPRTPTGKLLRRELKDRHREQPHSGHAVRTGRA
>Mature_526_residues
TTLHDWATVAPQKPALIQAESGHVITYGELDARAIRAAQWLVSRGLEAGDCIAVLVENHPGLIEIASAARRVGLYYTAVN
THLSPAEAAYVLKDCGAKLLIASPAMLPLVEAVRAADAHALDLPVFVLAGASNLPPGFEAYEAAISAFAPDATLPPRPVG
RDMLYSSGTTGHPKGIRRPLTPYEDRDKPELEIIAWRRAFGFDENTVYLSTAPFYHAAPLRYIMRTLDVGGTCVAMSKYD
AEGALAAVERYRVTHSQWVPTMFVRLLGLPDEARRRYDLSSMKVAIHAAAPCPVHVKQAMLDWWGDILYEYYAGSEGAGT
TSIGPLEWRKYPGSVGRASAGVIHIVDNAGNELPPNEVGMIYFSGVATFSYHNAPEKTRSAYNDKGWATYGDLGYVNEEG
YLFLSDRRADLILSGGVNVYPQEIESVLMQHPSVADVAVIGVPDPELGEVPKAVIELRDGKAATPDLAQDIVDYCQGRLG
RLKLPRTVVFDERLPRTPTGKLLRRELKDRHREQPHSGHAVRTGRA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=26.9927536231884, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI38505220, Length=505, Percent_Identity=27.7227722772277, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=25.9328358208955, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=25.9328358208955, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI42544132, Length=481, Percent_Identity=27.4428274428274, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI115511026, Length=494, Percent_Identity=26.1133603238866, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI122937307, Length=509, Percent_Identity=27.7013752455796, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI227499619, Length=524, Percent_Identity=24.618320610687, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI157311624, Length=518, Percent_Identity=25.0965250965251, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI157311622, Length=518, Percent_Identity=25.0965250965251, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI58082049, Length=518, Percent_Identity=25.4826254826255, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13375727, Length=532, Percent_Identity=24.6240601503759, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI62865631, Length=544, Percent_Identity=23.5294117647059, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI13325055, Length=544, Percent_Identity=23.5294117647059, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI8923896, Length=545, Percent_Identity=22.9357798165138, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI28416953, Length=530, Percent_Identity=24.1509433962264, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI253970489, Length=368, Percent_Identity=25, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI13325057, Length=535, Percent_Identity=22.9906542056075, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI221142682, Length=525, Percent_Identity=27.8095238095238, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=27.6119402985075, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=27.6315789473684, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1790505, Length=531, Percent_Identity=23.5404896421846, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786810, Length=378, Percent_Identity=26.4550264550265, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786529, Length=562, Percent_Identity=24.9110320284698, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=512, Percent_Identity=25.9765625, Blast_Score=168, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17559526, Length=508, Percent_Identity=30.3149606299213, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32563687, Length=493, Percent_Identity=26.9776876267748, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17558820, Length=501, Percent_Identity=26.7465069860279, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71994703, Length=538, Percent_Identity=25.4646840148699, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=541, Percent_Identity=25.3234750462107, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71994690, Length=541, Percent_Identity=25.3234750462107, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560140, Length=518, Percent_Identity=24.7104247104247, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=555, Percent_Identity=27.3873873873874, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=358, Percent_Identity=30.7262569832402, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=360, Percent_Identity=29.4444444444444, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17551278, Length=555, Percent_Identity=25.045045045045, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115534440, Length=548, Percent_Identity=22.2627737226277, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=211, Percent_Identity=30.3317535545024, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=347, Percent_Identity=26.8011527377522, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=570, Percent_Identity=24.3859649122807, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=559, Percent_Identity=23.613595706619, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355181, Length=519, Percent_Identity=27.9383429672447, Blast_Score=154, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18859661, Length=507, Percent_Identity=26.0355029585799, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21356441, Length=510, Percent_Identity=25.0980392156863, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24648253, Length=515, Percent_Identity=25.4368932038835, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24648255, Length=515, Percent_Identity=25.631067961165, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356947, Length=514, Percent_Identity=23.7354085603113, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648260, Length=509, Percent_Identity=26.1296660117878, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281365686, Length=482, Percent_Identity=26.5560165975104, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21358303, Length=484, Percent_Identity=24.3801652892562, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653035, Length=534, Percent_Identity=23.4082397003745, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161076582, Length=537, Percent_Identity=23.8361266294227, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=24.3564356435644, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581924, Length=554, Percent_Identity=23.6462093862816, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI20130357, Length=531, Percent_Identity=25.0470809792844, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24656500, Length=513, Percent_Identity=24.1715399610136, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI62472339, Length=534, Percent_Identity=23.9700374531835, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667955, Length=534, Percent_Identity=23.9700374531835, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355225, Length=537, Percent_Identity=23.6499068901304, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648257, Length=512, Percent_Identity=24.4140625, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667959, Length=362, Percent_Identity=24.8618784530387, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57558; Mature: 57427

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLHDWATVAPQKPALIQAESGHVITYGELDARAIRAAQWLVSRGLEAGDCIAVLVENH
CCCCCCHHHCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
PGLIEIASAARRVGLYYTAVNTHLSPAEAAYVLKDCGAKLLIASPAMLPLVEAVRAADAH
CCEEHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCC
ALDLPVFVLAGASNLPPGFEAYEAAISAFAPDATLPPRPVGRDMLYSSGTTGHPKGIRRP
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
LTPYEDRDKPELEIIAWRRAFGFDENTVYLSTAPFYHAAPLRYIMRTLDVGGTCVAMSKY
CCCCCCCCCCCEEEEEEHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECC
DAEGALAAVERYRVTHSQWVPTMFVRLLGLPDEARRRYDLSSMKVAIHAAAPCPVHVKQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHH
MLDWWGDILYEYYAGSEGAGTTSIGPLEWRKYPGSVGRASAGVIHIVDNAGNELPPNEVG
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEE
MIYFSGVATFSYHNAPEKTRSAYNDKGWATYGDLGYVNEEGYLFLSDRRADLILSGGVNV
EEEEECEEEEEECCCCHHHHHHCCCCCCEEECCCEEECCCCEEEEECCCCEEEEECCCCC
YPQEIESVLMQHPSVADVAVIGVPDPELGEVPKAVIELRDGKAATPDLAQDIVDYCQGRL
CHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCC
GRLKLPRTVVFDERLPRTPTGKLLRRELKDRHREQPHSGHAVRTGRA
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCC
>Mature Secondary Structure 
TTLHDWATVAPQKPALIQAESGHVITYGELDARAIRAAQWLVSRGLEAGDCIAVLVENH
CCCCCHHHCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEEECC
PGLIEIASAARRVGLYYTAVNTHLSPAEAAYVLKDCGAKLLIASPAMLPLVEAVRAADAH
CCEEHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCC
ALDLPVFVLAGASNLPPGFEAYEAAISAFAPDATLPPRPVGRDMLYSSGTTGHPKGIRRP
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCC
LTPYEDRDKPELEIIAWRRAFGFDENTVYLSTAPFYHAAPLRYIMRTLDVGGTCVAMSKY
CCCCCCCCCCCEEEEEEHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECC
DAEGALAAVERYRVTHSQWVPTMFVRLLGLPDEARRRYDLSSMKVAIHAAAPCPVHVKQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHH
MLDWWGDILYEYYAGSEGAGTTSIGPLEWRKYPGSVGRASAGVIHIVDNAGNELPPNEVG
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCEE
MIYFSGVATFSYHNAPEKTRSAYNDKGWATYGDLGYVNEEGYLFLSDRRADLILSGGVNV
EEEEECEEEEEECCCCHHHHHHCCCCCCEEECCCEEECCCCEEEEECCCCEEEEECCCCC
YPQEIESVLMQHPSVADVAVIGVPDPELGEVPKAVIELRDGKAATPDLAQDIVDYCQGRL
CHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCC
GRLKLPRTVVFDERLPRTPTGKLLRRELKDRHREQPHSGHAVRTGRA
CCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]