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The map label for this gene is cynR [H]

Identifier: 94314054

GI number: 94314054

Start: 1832913

End: 1833803

Strand: Direct

Name: cynR [H]

Synonym: Rmet_5135

Alternate gene names: 94314054

Gene position: 1832913-1833803 (Clockwise)

Preceding gene: 94314046

Following gene: 94314055

Centisome position: 71.04

GC content: 65.88

Gene sequence:

>891_bases
ATGGAGACTCGCGATCTGGAATACGTCCTGGCTGTGGAAACCCACGGTGGCATCGGGCGCGCCGCCGAGGCACTGGACAT
GACCCAGCCGGCACTTACCAAGGCCGTGCAGCGCGTGGAGGCGCAACTGGGCGTACCGCTGTTCGAACGCACGACGCTGG
GTATGCGCGTCACGCAGGCGGGAGCGGTCTTTCTGGAACGGGCACGCCGGATCCGGCTGGAATATGAAGATGCAATCAAG
GAAATGCGCGGCATCCAGACCGGCGAACAAGGGATGCTGCGCCTCGGCTATTCCCCTTCGATGCCCAATGCGCTGGTGCT
GGGCGCGTGTCGGCAACTGATTCGCGAGCGGCCAGTGGCACGACTGCGCCTGACGATGCGTGTGGCGCGCGAGTTGATGG
ACCTGCTGCAGGCCGGCGAACTCGACCTGGCCATCGCCCCGTTGCCGCGTCAGGCGAACCCGGCCCTGGTATCGCGTGAA
CTGTTCACCGACCGCCTGGCCGTGGTGGCCGACGAGAATCATCCGTTGCTACGACGACGCAACCTGACGCTGGCGGACCT
GACCGACCAGCAGTGGCTGCTGCCTAGCTCGCACGTGCTGATCCGCCAGCAGATCGACGCCGCCTTCACGGAACTGGGCT
TGCCCGCACCGATCCTGCGCGTGGAGACCGACTTCGGCAGCACGGCCCTCTTCGATCTGGTGCGCGGCACCGAAATGCTC
TGCATCGCGGGATCAACCAGCAATGGCGCACAGACGGGCCTGCGCCCCATCGCACTGCGACCTGATGCCCTGGACCTGGG
CCGACGGGTAGGCGTGATGTCGCGGGCGGGTGCCTATCTCTCGCCGCTGGCCCTGCGCATGATCGGTATTTTCGAGGCGC
GGATGCAATGA

Upstream 100 bases:

>100_bases
CCTTGCAACGTGTCTGGTATTTGTGCGCCCAGTCTGTAAGATGACGGGCATCACTACAATGCATTGACTGGAATAAAGTC
ATAAATACTAGGAATGTGCG

Downstream 100 bases:

>100_bases
GGGACGATCGGGACCCGCGCATTACACTGCAGCCTATGAGCCGCAGCCACCCGCAACCGCATCTTCAGGATTCCCTCACC
GCCTACTACTGGAGTGGCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
METRDLEYVLAVETHGGIGRAAEALDMTQPALTKAVQRVEAQLGVPLFERTTLGMRVTQAGAVFLERARRIRLEYEDAIK
EMRGIQTGEQGMLRLGYSPSMPNALVLGACRQLIRERPVARLRLTMRVARELMDLLQAGELDLAIAPLPRQANPALVSRE
LFTDRLAVVADENHPLLRRRNLTLADLTDQQWLLPSSHVLIRQQIDAAFTELGLPAPILRVETDFGSTALFDLVRGTEML
CIAGSTSNGAQTGLRPIALRPDALDLGRRVGVMSRAGAYLSPLALRMIGIFEARMQ

Sequences:

>Translated_296_residues
METRDLEYVLAVETHGGIGRAAEALDMTQPALTKAVQRVEAQLGVPLFERTTLGMRVTQAGAVFLERARRIRLEYEDAIK
EMRGIQTGEQGMLRLGYSPSMPNALVLGACRQLIRERPVARLRLTMRVARELMDLLQAGELDLAIAPLPRQANPALVSRE
LFTDRLAVVADENHPLLRRRNLTLADLTDQQWLLPSSHVLIRQQIDAAFTELGLPAPILRVETDFGSTALFDLVRGTEML
CIAGSTSNGAQTGLRPIALRPDALDLGRRVGVMSRAGAYLSPLALRMIGIFEARMQ
>Mature_296_residues
METRDLEYVLAVETHGGIGRAAEALDMTQPALTKAVQRVEAQLGVPLFERTTLGMRVTQAGAVFLERARRIRLEYEDAIK
EMRGIQTGEQGMLRLGYSPSMPNALVLGACRQLIRERPVARLRLTMRVARELMDLLQAGELDLAIAPLPRQANPALVSRE
LFTDRLAVVADENHPLLRRRNLTLADLTDQQWLLPSSHVLIRQQIDAAFTELGLPAPILRVETDFGSTALFDLVRGTEML
CIAGSTSNGAQTGLRPIALRPDALDLGRRVGVMSRAGAYLSPLALRMIGIFEARMQ

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=265, Percent_Identity=30.5660377358491, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790399, Length=212, Percent_Identity=31.1320754716981, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787879, Length=203, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081904, Length=224, Percent_Identity=28.125, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788887, Length=266, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787601, Length=196, Percent_Identity=26.0204081632653, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787530, Length=227, Percent_Identity=25.9911894273128, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789506, Length=228, Percent_Identity=24.1228070175439, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367136, Length=188, Percent_Identity=28.1914893617021, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI145693105, Length=173, Percent_Identity=24.8554913294798, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=215, Percent_Identity=26.046511627907, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32684; Mature: 32684

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRDLEYVLAVETHGGIGRAAEALDMTQPALTKAVQRVEAQLGVPLFERTTLGMRVTQA
CCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEHHHH
GAVFLERARRIRLEYEDAIKEMRGIQTGEQGMLRLGYSPSMPNALVLGACRQLIRERPVA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHH
RLRLTMRVARELMDLLQAGELDLAIAPLPRQANPALVSRELFTDRLAVVADENHPLLRRR
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCEEEEECCCCCCHHHC
NLTLADLTDQQWLLPSSHVLIRQQIDAAFTELGLPAPILRVETDFGSTALFDLVRGTEML
CCEEEECCCCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCEEE
CIAGSTSNGAQTGLRPIALRPDALDLGRRVGVMSRAGAYLSPLALRMIGIFEARMQ
EEECCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
METRDLEYVLAVETHGGIGRAAEALDMTQPALTKAVQRVEAQLGVPLFERTTLGMRVTQA
CCCCCEEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCEEHHHH
GAVFLERARRIRLEYEDAIKEMRGIQTGEQGMLRLGYSPSMPNALVLGACRQLIRERPVA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHH
RLRLTMRVARELMDLLQAGELDLAIAPLPRQANPALVSRELFTDRLAVVADENHPLLRRR
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCEEEEECCCCCCHHHC
NLTLADLTDQQWLLPSSHVLIRQQIDAAFTELGLPAPILRVETDFGSTALFDLVRGTEML
CCEEEECCCCCEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCEEE
CIAGSTSNGAQTGLRPIALRPDALDLGRRVGVMSRAGAYLSPLALRMIGIFEARMQ
EEECCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]