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The map label for this gene is yngJ [H]

Identifier: 94314052

GI number: 94314052

Start: 1830065

End: 1831240

Strand: Reverse

Name: yngJ [H]

Synonym: Rmet_5133

Alternate gene names: 94314052

Gene position: 1831240-1830065 (Counterclockwise)

Preceding gene: 94314053

Following gene: 94314051

Centisome position: 70.98

GC content: 64.37

Gene sequence:

>1176_bases
ATGTTCGAACATCTGACCAACCCCGTGCTGCTGGATACGCGCGTAGCGATCCAGCGCTTCATCGACGAGGAACTGCGGCC
GCTGGAGCGCGAGCTGGATCTGGGCGCGGAAGACCCGTGGCCGCGCGACGTGCTGCGCAACGTCTGGCGCCGTTCCAGCG
AACTCGGGCTCTACGCGGCATGCCTGCCCGTGGAACTTGGCGGCAAGGGTCTCAATATCGCGGAGCAGTGCGCGCTCAAG
GCCGATCTGGCCGCCAGCGGGGCGGTGCTGGCGCCGCACGTGCTGGGCGATCTCGGCGGGCCGCCCCGCGTGGGCAACAT
GCTCAAATACGCGACGCCTGACCAGATCGAACGCTACTTCCAACCGGTAATTCGCGGAGAGAAGTCCACGTGCTTCGCGC
TGACGGAGACGCATTCTGGCTCGGATGTGCAGAGCATTCGGACCAGTGCCGTCGTAGATGGCGATGATCTCGTCATCAAC
GGAGAGAAGCACTACATCAGCGGTGCGCCGTTCGCGGACTTTGCCATCGTGATGTGCGTGACCGATGCCGCCACCGCGCC
ACCGTCGATCACGGCGGTGCTCGTCGACCTTGATTTGCCGGGCGTGACCGTCAGTACCGAGTACGTACCGATGTCTGGCC
AGCATATTGATGGCGATATCCGCTTCGAGAACGTGCGTGTGCCGCGTGCGAACATCTTCGGTGGCGAAGGACGTGGCTTC
AAACTCGGCATGTCGCGCATCAACGTCAACCGGCTGCTGCATTGTCCGTCGATGCTGGGGCTGGCCATGAGCGCGTACAA
GGCGTCGGTACAGTACGCGGGCGCGCGCCGCCAGTTTGGCGGCCCGATCGCGCGTTTCCAGGCCATCCAGCACATGCTGG
CCGATATGGCGGCCGCGCTGTGGGCGTGCGAGAGCATGATCGCGCAGACAGCGATGCTTGCCGATGCCGGCGCTGACCTG
CGCATGAAGGCGGCGGCGTGCAAGCTGTTCGTGTCGGAGCGCTGCTTCGAGGTGGCCGACAAGGCAGTGCAGATTCACGG
CAATGTGGGCGTGACGCGCGGCCATCCGGTGGAGCAGACCTGGCGCAAGCTGCGCATGTTCCGCATCTTTACGGGCACCA
GCGAGATCCAGCGGAATACGATCGCCAAGGCGATTCTCGATCCCACTGGGGCATGA

Upstream 100 bases:

>100_bases
GCCGAGCTGCCCAAGAACGCAAGCGGAAAGCTGGCGCGCAAAGTCGTGCGGGAACAGTACTGGGTGGGCGCCAGCCGCCG
CGTCAATTAAGGGGCACATC

Downstream 100 bases:

>100_bases
CCATCTGGCCTGATCGGGTCTGACCTGACCGACGGGCCTCAACGCATTTGCGTGGCGACATTGCGATACAAGACAATGCG
CCGCATGACAGGAGACAACC

Product: acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MFEHLTNPVLLDTRVAIQRFIDEELRPLERELDLGAEDPWPRDVLRNVWRRSSELGLYAACLPVELGGKGLNIAEQCALK
ADLAASGAVLAPHVLGDLGGPPRVGNMLKYATPDQIERYFQPVIRGEKSTCFALTETHSGSDVQSIRTSAVVDGDDLVIN
GEKHYISGAPFADFAIVMCVTDAATAPPSITAVLVDLDLPGVTVSTEYVPMSGQHIDGDIRFENVRVPRANIFGGEGRGF
KLGMSRINVNRLLHCPSMLGLAMSAYKASVQYAGARRQFGGPIARFQAIQHMLADMAAALWACESMIAQTAMLADAGADL
RMKAAACKLFVSERCFEVADKAVQIHGNVGVTRGHPVEQTWRKLRMFRIFTGTSEIQRNTIAKAILDPTGA

Sequences:

>Translated_391_residues
MFEHLTNPVLLDTRVAIQRFIDEELRPLERELDLGAEDPWPRDVLRNVWRRSSELGLYAACLPVELGGKGLNIAEQCALK
ADLAASGAVLAPHVLGDLGGPPRVGNMLKYATPDQIERYFQPVIRGEKSTCFALTETHSGSDVQSIRTSAVVDGDDLVIN
GEKHYISGAPFADFAIVMCVTDAATAPPSITAVLVDLDLPGVTVSTEYVPMSGQHIDGDIRFENVRVPRANIFGGEGRGF
KLGMSRINVNRLLHCPSMLGLAMSAYKASVQYAGARRQFGGPIARFQAIQHMLADMAAALWACESMIAQTAMLADAGADL
RMKAAACKLFVSERCFEVADKAVQIHGNVGVTRGHPVEQTWRKLRMFRIFTGTSEIQRNTIAKAILDPTGA
>Mature_391_residues
MFEHLTNPVLLDTRVAIQRFIDEELRPLERELDLGAEDPWPRDVLRNVWRRSSELGLYAACLPVELGGKGLNIAEQCALK
ADLAASGAVLAPHVLGDLGGPPRVGNMLKYATPDQIERYFQPVIRGEKSTCFALTETHSGSDVQSIRTSAVVDGDDLVIN
GEKHYISGAPFADFAIVMCVTDAATAPPSITAVLVDLDLPGVTVSTEYVPMSGQHIDGDIRFENVRVPRANIFGGEGRGF
KLGMSRINVNRLLHCPSMLGLAMSAYKASVQYAGARRQFGGPIARFQAIQHMLADMAAALWACESMIAQTAMLADAGADL
RMKAAACKLFVSERCFEVADKAVQIHGNVGVTRGHPVEQTWRKLRMFRIFTGTSEIQRNTIAKAILDPTGA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=383, Percent_Identity=32.8981723237598, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI226958412, Length=378, Percent_Identity=30.6878306878307, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI76496475, Length=382, Percent_Identity=34.2931937172775, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4557235, Length=382, Percent_Identity=34.2931937172775, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4501859, Length=376, Percent_Identity=30.5851063829787, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI187960098, Length=376, Percent_Identity=30.8510638297872, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4557231, Length=376, Percent_Identity=30.8510638297872, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI226958414, Length=346, Percent_Identity=30.635838150289, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI7656849, Length=375, Percent_Identity=29.6, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI21361497, Length=389, Percent_Identity=31.6195372750643, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI4501857, Length=383, Percent_Identity=26.8929503916449, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI48976061, Length=393, Percent_Identity=28.498727735369, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI211971008, Length=393, Percent_Identity=28.498727735369, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI38505218, Length=396, Percent_Identity=26.2626262626263, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503943, Length=280, Percent_Identity=26.7857142857143, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI7669494, Length=191, Percent_Identity=31.413612565445, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786223, Length=336, Percent_Identity=31.25, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081958, Length=353, Percent_Identity=28.6118980169972, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87081702, Length=340, Percent_Identity=26.4705882352941, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17538396, Length=376, Percent_Identity=31.1170212765957, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17570075, Length=378, Percent_Identity=29.8941798941799, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17508101, Length=370, Percent_Identity=31.0810810810811, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17534899, Length=391, Percent_Identity=30.9462915601023, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17569725, Length=390, Percent_Identity=30.2564102564103, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71985184, Length=378, Percent_Identity=31.7460317460317, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17506239, Length=370, Percent_Identity=28.6486486486486, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17551932, Length=341, Percent_Identity=30.791788856305, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17533517, Length=389, Percent_Identity=29.5629820051414, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17505929, Length=342, Percent_Identity=29.5321637426901, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71985192, Length=325, Percent_Identity=30.1538461538462, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71990804, Length=290, Percent_Identity=28.9655172413793, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17562446, Length=361, Percent_Identity=26.5927977839335, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71982178, Length=274, Percent_Identity=27.007299270073, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531909, Length=242, Percent_Identity=27.6859504132231, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32563615, Length=229, Percent_Identity=27.0742358078603, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534353, Length=290, Percent_Identity=25.5172413793103, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24660351, Length=376, Percent_Identity=31.9148936170213, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24646207, Length=386, Percent_Identity=32.1243523316062, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24666513, Length=374, Percent_Identity=31.283422459893, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI281363737, Length=343, Percent_Identity=33.8192419825073, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI21356377, Length=371, Percent_Identity=30.9973045822102, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI21355753, Length=372, Percent_Identity=30.3763440860215, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19920834, Length=293, Percent_Identity=25.938566552901, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 42475; Mature: 42475

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00072 ACYL_COA_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEHLTNPVLLDTRVAIQRFIDEELRPLERELDLGAEDPWPRDVLRNVWRRSSELGLYAA
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEE
CLPVELGGKGLNIAEQCALKADLAASGAVLAPHVLGDLGGPPRVGNMLKYATPDQIERYF
EEEEEECCCCCCHHHHHHHHHHHCCCCCEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QPVIRGEKSTCFALTETHSGSDVQSIRTSAVVDGDDLVINGEKHYISGAPFADFAIVMCV
HHHHCCCCCEEEEEEECCCCCHHHHHHHHHEECCCCEEECCCCCEECCCCHHHHEEEEEE
TDAATAPPSITAVLVDLDLPGVTVSTEYVPMSGQHIDGDIRFENVRVPRANIFGGEGRGF
ECCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCE
KLGMSRINVNRLLHCPSMLGLAMSAYKASVQYAGARRQFGGPIARFQAIQHMLADMAAAL
EEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHH
WACESMIAQTAMLADAGADLRMKAAACKLFVSERCFEVADKAVQIHGNVGVTRGHPVEQT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHH
WRKLRMFRIFTGTSEIQRNTIAKAILDPTGA
HHHHHHHHEECCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFEHLTNPVLLDTRVAIQRFIDEELRPLERELDLGAEDPWPRDVLRNVWRRSSELGLYAA
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEE
CLPVELGGKGLNIAEQCALKADLAASGAVLAPHVLGDLGGPPRVGNMLKYATPDQIERYF
EEEEEECCCCCCHHHHHHHHHHHCCCCCEECHHHHHCCCCCCCCCCCCCCCCHHHHHHHH
QPVIRGEKSTCFALTETHSGSDVQSIRTSAVVDGDDLVINGEKHYISGAPFADFAIVMCV
HHHHCCCCCEEEEEEECCCCCHHHHHHHHHEECCCCEEECCCCCEECCCCHHHHEEEEEE
TDAATAPPSITAVLVDLDLPGVTVSTEYVPMSGQHIDGDIRFENVRVPRANIFGGEGRGF
ECCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCE
KLGMSRINVNRLLHCPSMLGLAMSAYKASVQYAGARRQFGGPIARFQAIQHMLADMAAAL
EEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHH
WACESMIAQTAMLADAGADLRMKAAACKLFVSERCFEVADKAVQIHGNVGVTRGHPVEQT
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHH
WRKLRMFRIFTGTSEIQRNTIAKAILDPTGA
HHHHHHHHEECCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]