BacMap

Definition	Cupriavidus metallidurans CH34 megaplasmid, complete sequence.
Accession	NC_007974
Length	2,580,084

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The map label for this gene is linX [H]

Identifier: 94313874

GI number: 94313874

Start: 1617383

End: 1618192

Strand: Reverse

Name: linX [H]

Synonym: Rmet_4952

Alternate gene names: 94313874

Gene position: 1618192-1617383 (Counterclockwise)

Preceding gene: 94313875

Following gene: 94313873

Centisome position: 62.72

GC content: 69.01

Gene sequence:

>810_bases
ATGGATTTATCCAGGCATGGCGGCACGCTGGCCGGCAAGCGCATTCTCGTCGTCGGAGGCGGGCAGGGGGGTGACGAGCA
GATGGACGAGCCGGCCGGCAACGGGCGGGCCGCCTGTTGCCTGCTCGCCCGCGAAGGCGCGCATGTTGCCTGCGCGGACC
GTTCTGGGGCGGCGGCGCAGCGCACGGTCGCACGCATCGCCGCGGATGGTGGACGCGCCGCTGCCATCGAGGCCGATGTC
TCGCGCACGGCAGACATCCACGCGATGATCGACCAGGCCGTCGAGGCGCTGGGCGGTCTGGATGCTCTGGTGCTCAACGT
CGGCATCAGCCATCGGCTGCCGCTCGCCCGGCTGACCGAGGAGAGCTGGGATGCTGTCCTCGGCATCAACCTCAAGGCAC
ACATGCTGTGCGTGCAGGTGGCGTTGCCCCGCATGACGGAGGGTGGTGCCATCGTCTTTGTTTCGTCGGTGGCGGCACGT
CGGCCGAATGGGCGGAACCCTGCCTATGAAGTCTCGAAGGCAGGCCTTGGCGCGTTATGCCGCGCCGCGGCGCTCGAAGG
TCAGCCAAGCGGCATCCGCGCCAACGTGGTCATGCTCGGCCTTATCGACACCCCAATGGGGCGGGCGGCATCGGCTGCGC
GGCCGGACCGCACCAGCGCGCCGCTGCCGTTTGGCCGGCAAGGCACCGCGTGGGAGGTGGCGGCAGCGATTCGCTTCCTG
GTGTCGGACGAATCGCGCTATGTGAATGCGATCGAACTCCCAGTGGATGGCGGGTTGTCGGCCGGCATCCAGCTTGCACC
GCGCGCCTGA

Upstream 100 bases:

>100_bases
CGTTTCCTGCTGACCGCGCCGCCGTTGCGCCTGCCGGGTGCGGTGGGTTCGCCAGTGACGCCCGTGGCAACGATCTGATT
CCCGTTCGACAAGGAACACC

Downstream 100 bases:

>100_bases
ACGACTGATCTCGAGGAACAACCGGAGATGACCTTGGTTTCGACCCTGTCGCGGCTTGGCCGCATGATGGCGATGGTTGC
ACTGTTCACGGCCGCCGCGC

Product: putative short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 2,5-DDOL dehydrogenase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MDLSRHGGTLAGKRILVVGGGQGGDEQMDEPAGNGRAACCLLAREGAHVACADRSGAAAQRTVARIAADGGRAAAIEADV
SRTADIHAMIDQAVEALGGLDALVLNVGISHRLPLARLTEESWDAVLGINLKAHMLCVQVALPRMTEGGAIVFVSSVAAR
RPNGRNPAYEVSKAGLGALCRAAALEGQPSGIRANVVMLGLIDTPMGRAASAARPDRTSAPLPFGRQGTAWEVAAAIRFL
VSDESRYVNAIELPVDGGLSAGIQLAPRA

Sequences:

>Translated_269_residues
MDLSRHGGTLAGKRILVVGGGQGGDEQMDEPAGNGRAACCLLAREGAHVACADRSGAAAQRTVARIAADGGRAAAIEADV
SRTADIHAMIDQAVEALGGLDALVLNVGISHRLPLARLTEESWDAVLGINLKAHMLCVQVALPRMTEGGAIVFVSSVAAR
RPNGRNPAYEVSKAGLGALCRAAALEGQPSGIRANVVMLGLIDTPMGRAASAARPDRTSAPLPFGRQGTAWEVAAAIRFL
VSDESRYVNAIELPVDGGLSAGIQLAPRA
>Mature_269_residues
MDLSRHGGTLAGKRILVVGGGQGGDEQMDEPAGNGRAACCLLAREGAHVACADRSGAAAQRTVARIAADGGRAAAIEADV
SRTADIHAMIDQAVEALGGLDALVLNVGISHRLPLARLTEESWDAVLGINLKAHMLCVQVALPRMTEGGAIVFVSSVAAR
RPNGRNPAYEVSKAGLGALCRAAALEGQPSGIRANVVMLGLIDTPMGRAASAARPDRTSAPLPFGRQGTAWEVAAAIRFL
VSDESRYVNAIELPVDGGLSAGIQLAPRA

Specific function: Catalyzes the degradation of 2,5-dichloro-2,5- cyclohexadiene-1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ). LinX appears not to be involved in gamma-HCH degradation pathway [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI15277342, Length=239, Percent_Identity=33.4728033472803, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI5031737, Length=223, Percent_Identity=32.7354260089686, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI32483357, Length=222, Percent_Identity=31.5315315315315, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI40254992, Length=230, Percent_Identity=33.0434782608696, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI33667109, Length=172, Percent_Identity=36.046511627907, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI126723750, Length=232, Percent_Identity=30.1724137931034, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI126723191, Length=163, Percent_Identity=34.3558282208589, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI66933014, Length=230, Percent_Identity=30, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI7705925, Length=258, Percent_Identity=31.7829457364341, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI59889578, Length=200, Percent_Identity=30.5, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI302344755, Length=113, Percent_Identity=39.8230088495575, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4504505, Length=199, Percent_Identity=28.1407035175879, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31542939, Length=204, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI2367175, Length=238, Percent_Identity=38.2352941176471, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1787335, Length=229, Percent_Identity=32.7510917030568, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788459, Length=243, Percent_Identity=29.2181069958848, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789208, Length=206, Percent_Identity=30.0970873786408, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=30.6451612903226, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790717, Length=259, Percent_Identity=28.1853281853282, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=31.0606060606061, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786812, Length=265, Percent_Identity=28.6792452830189, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI2367365, Length=255, Percent_Identity=30.9803921568627, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=28.6821705426357, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=30.7086614173228, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25147288, Length=256, Percent_Identity=32.8125, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17560676, Length=236, Percent_Identity=29.6610169491525, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=248, Percent_Identity=31.0483870967742, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71994604, Length=237, Percent_Identity=29.957805907173, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994600, Length=242, Percent_Identity=29.3388429752066, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=29.9610894941634, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=251, Percent_Identity=30.2788844621514, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538480, Length=249, Percent_Identity=29.718875502008, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=238, Percent_Identity=26.4705882352941, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551412, Length=251, Percent_Identity=28.2868525896414, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17565030, Length=248, Percent_Identity=27.8225806451613, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=212, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=259, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=276, Percent_Identity=25.7246376811594, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=261, Percent_Identity=28.3524904214559, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508651, Length=242, Percent_Identity=26.8595041322314, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=220, Percent_Identity=25.9090909090909, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24639444, Length=261, Percent_Identity=36.7816091954023, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI23397609, Length=267, Percent_Identity=33.7078651685393, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643142, Length=254, Percent_Identity=33.4645669291339, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21357041, Length=245, Percent_Identity=32.6530612244898, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24644339, Length=267, Percent_Identity=30.7116104868914, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21355319, Length=234, Percent_Identity=29.9145299145299, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571526, Length=245, Percent_Identity=31.4285714285714, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18859837, Length=193, Percent_Identity=32.1243523316062, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644337, Length=226, Percent_Identity=30.0884955752212, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24649181, Length=181, Percent_Identity=29.2817679558011, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27593; Mature: 27593

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSRHGGTLAGKRILVVGGGQGGDEQMDEPAGNGRAACCLLAREGAHVACADRSGAAAQ
CCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCHHH
RTVARIAADGGRAAAIEADVSRTADIHAMIDQAVEALGGLDALVLNVGISHRLPLARLTE
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHH
ESWDAVLGINLKAHMLCVQVALPRMTEGGAIVFVSSVAARRPNGRNPAYEVSKAGLGALC
CCCCEEEECCHHHHHHEEEHHCCCCCCCCEEEEEEEHHHCCCCCCCCCHHHHHCCHHHHH
RAAALEGQPSGIRANVVMLGLIDTPMGRAASAARPDRTSAPLPFGRQGTAWEVAAAIRFL
HHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VSDESRYVNAIELPVDGGLSAGIQLAPRA
HCCCCCEEEEEEECCCCCCCCCEEECCCC
>Mature Secondary Structure
MDLSRHGGTLAGKRILVVGGGQGGDEQMDEPAGNGRAACCLLAREGAHVACADRSGAAAQ
CCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCCCCCEEEEEEECCCCEEEEECCCCCHHH
RTVARIAADGGRAAAIEADVSRTADIHAMIDQAVEALGGLDALVLNVGISHRLPLARLTE
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCHHHHHH
ESWDAVLGINLKAHMLCVQVALPRMTEGGAIVFVSSVAARRPNGRNPAYEVSKAGLGALC
CCCCEEEECCHHHHHHEEEHHCCCCCCCCEEEEEEEHHHCCCCCCCCCHHHHHCCHHHHH
RAAALEGQPSGIRANVVMLGLIDTPMGRAASAARPDRTSAPLPFGRQGTAWEVAAAIRFL
HHHHCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VSDESRYVNAIELPVDGGLSAGIQLAPRA
HCCCCCEEEEEEECCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7515041; 12450824 [H]