Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 94313810

GI number: 94313810

Start: 1549938

End: 1551380

Strand: Reverse

Name: lpdA [H]

Synonym: Rmet_4888

Alternate gene names: 94313810

Gene position: 1551380-1549938 (Counterclockwise)

Preceding gene: 94313811

Following gene: 94313809

Centisome position: 60.13

GC content: 65.9

Gene sequence:

>1443_bases
ATGAACACGAACCAGAATGCCCAGACCGCTCAAACTGTCCAGACCGTCCAGACTGATGTGGCCATCATCGGCGCGGGCAC
TGCCGGCCTGGCCGCCTACCGCGCGGCCATTGCCGCCGGCAAGCGCGCCGTGATCATCGAGGGCGGGGCGTATGGCACGA
CCTGCGCCCGCGTGGGCTGCATGCCGTCGAAGCTGCTGATTGCCGCGGCGGAAGCCGCGCACGAAGCGCGTCACACTGCT
GCATTTGGCGTGCACGTGGATGGCCCGGTCCGTATCGACGGCAGGGAAGTCATGCAGCGCGTGCGGAGCGAACGCGACCG
CTTTGTCGGCTTCGTGGTGCGCGGCGTGGAGAGCATTCCCCAGGACGACCGCCTGATCGGCCGGGCGCGGTTCATCGACA
ACACGATCCTCCAGGTTGACGACCACACCGTCGTGCGCGCAAGCCGCGTGGTAATCGCCACGGGCTCGCGCCCCGCCGTA
CCGCCGTCCTTTGGCGTGTTCGGCGATCGCCTGATCGTCAATGACGATGTGTTCTCGTGGGAAGACCTGCCCCAAAGCGT
GGCCGTGTTCGGCCCGGGCGTGATTGGTTTGGAACTGGGGCAAGCACTGTCGCGGCTTGGCGTGCGGGTGCGCGTGTTTG
GTGTCGGTGGCGGCGTCGGTCCGTTGTCGGACCCCGAGATTCGTGCTTACGCTGCCAAGTCCTTCAACCAGGAATTCTCG
CTGTTGGCGGATGCGCGCGTGCTTGAGATGCGCCGCGAGGGCGACGACGCCGTTATCACGTTCGAAGACGCCGACGGCCA
GACCGTGACGGAGCGCTTTGCCTACGTGCTGGCCGCCACGGGTCGCCGGCCCAATGTCGATACGCTTGCGCTGGGCAACA
CCGCGCTGGAACTCGATGGGCGGGGCGTACCGACGTTCGACCCGGTGACGCTCCAGTGCGGTGAATCGCCGATCTTCATC
GCTGGCGATGCCAATAACGTCCTGCCGCTGCTGCATGAGGCGGCCGACGAAGGCAAGATTGCTGGCGAAAACGCTGCGCT
CTGGCCGAAGGTCGGGCCGCTGACGCGGCGCGCGCCGATCGCGGTGGTGTTCTCCGATCCGCAGATCGCGATGGTCGGCA
AGCGTTTTGCCGAACTGACACCGGGCAGCTTCGTGACTGGCGAGGTCAGCTTCGAGGATCAGGGCCGCAGCCGTGTGATG
CTCAAGAACCGTGGGCTGATGCACGTCTACGCGGATCGCGCCACAGGCCGCTTTATTGGCGCTGAGTGGATTGGCCCGCG
CGCCGAGAATATCGCGCATCTGCTGGCGTGGTCGTACCAACAGGGGCTGACGATCGCCGCGATGCTGGCCATGCCTTTCT
ACCATCCCGTTGTGGAAGAGGGCTTGCGCACGGCGCTGCGTGATGCGCAAGCCAAGCTGGCGCAATTGCAGGAAGCCGCC
TGA

Upstream 100 bases:

>100_bases
GGGCACCCTCTCCCCCCTAGCAAGTCGTCGTATCCGATGGCGCCGCGTATGTGGCGGCGCCATCCCGTACGCCGATTGAC
GATCGATACAGGAAACGCAC

Downstream 100 bases:

>100_bases
TCGGACGGCAGCGTGAGCCTGGCTTGATCGGGAGGGCCGGAAGCGAAGTCTCGCTTCCGGCCCTTGTTCATTTGGCGGGA
TGCATATATAGAGATCGAGA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNTNQNAQTAQTVQTVQTDVAIIGAGTAGLAAYRAAIAAGKRAVIIEGGAYGTTCARVGCMPSKLLIAAAEAAHEARHTA
AFGVHVDGPVRIDGREVMQRVRSERDRFVGFVVRGVESIPQDDRLIGRARFIDNTILQVDDHTVVRASRVVIATGSRPAV
PPSFGVFGDRLIVNDDVFSWEDLPQSVAVFGPGVIGLELGQALSRLGVRVRVFGVGGGVGPLSDPEIRAYAAKSFNQEFS
LLADARVLEMRREGDDAVITFEDADGQTVTERFAYVLAATGRRPNVDTLALGNTALELDGRGVPTFDPVTLQCGESPIFI
AGDANNVLPLLHEAADEGKIAGENAALWPKVGPLTRRAPIAVVFSDPQIAMVGKRFAELTPGSFVTGEVSFEDQGRSRVM
LKNRGLMHVYADRATGRFIGAEWIGPRAENIAHLLAWSYQQGLTIAAMLAMPFYHPVVEEGLRTALRDAQAKLAQLQEAA

Sequences:

>Translated_480_residues
MNTNQNAQTAQTVQTVQTDVAIIGAGTAGLAAYRAAIAAGKRAVIIEGGAYGTTCARVGCMPSKLLIAAAEAAHEARHTA
AFGVHVDGPVRIDGREVMQRVRSERDRFVGFVVRGVESIPQDDRLIGRARFIDNTILQVDDHTVVRASRVVIATGSRPAV
PPSFGVFGDRLIVNDDVFSWEDLPQSVAVFGPGVIGLELGQALSRLGVRVRVFGVGGGVGPLSDPEIRAYAAKSFNQEFS
LLADARVLEMRREGDDAVITFEDADGQTVTERFAYVLAATGRRPNVDTLALGNTALELDGRGVPTFDPVTLQCGESPIFI
AGDANNVLPLLHEAADEGKIAGENAALWPKVGPLTRRAPIAVVFSDPQIAMVGKRFAELTPGSFVTGEVSFEDQGRSRVM
LKNRGLMHVYADRATGRFIGAEWIGPRAENIAHLLAWSYQQGLTIAAMLAMPFYHPVVEEGLRTALRDAQAKLAQLQEAA
>Mature_480_residues
MNTNQNAQTAQTVQTVQTDVAIIGAGTAGLAAYRAAIAAGKRAVIIEGGAYGTTCARVGCMPSKLLIAAAEAAHEARHTA
AFGVHVDGPVRIDGREVMQRVRSERDRFVGFVVRGVESIPQDDRLIGRARFIDNTILQVDDHTVVRASRVVIATGSRPAV
PPSFGVFGDRLIVNDDVFSWEDLPQSVAVFGPGVIGLELGQALSRLGVRVRVFGVGGGVGPLSDPEIRAYAAKSFNQEFS
LLADARVLEMRREGDDAVITFEDADGQTVTERFAYVLAATGRRPNVDTLALGNTALELDGRGVPTFDPVTLQCGESPIFI
AGDANNVLPLLHEAADEGKIAGENAALWPKVGPLTRRAPIAVVFSDPQIAMVGKRFAELTPGSFVTGEVSFEDQGRSRVM
LKNRGLMHVYADRATGRFIGAEWIGPRAENIAHLLAWSYQQGLTIAAMLAMPFYHPVVEEGLRTALRDAQAKLAQLQEAA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=27.1008403361345, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=23.1601731601732, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786307, Length=476, Percent_Identity=26.0504201680672, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082354, Length=474, Percent_Identity=25.9493670886076, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789915, Length=340, Percent_Identity=27.9411764705882, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081717, Length=453, Percent_Identity=23.6203090507726, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=29.2110874200426, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17557007, Length=489, Percent_Identity=22.6993865030675, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983419, Length=339, Percent_Identity=27.1386430678466, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983429, Length=339, Percent_Identity=27.1386430678466, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=490, Percent_Identity=26.734693877551, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325240, Length=486, Percent_Identity=24.4855967078189, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325166, Length=208, Percent_Identity=29.8076923076923, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=26.8498942917548, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=518, Percent_Identity=25.6756756756757, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24640553, Length=515, Percent_Identity=25.4368932038835, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24640549, Length=501, Percent_Identity=25.9481037924152, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17737741, Length=500, Percent_Identity=25.2, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51344; Mature: 51344

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNQNAQTAQTVQTVQTDVAIIGAGTAGLAAYRAAIAAGKRAVIIEGGAYGTTCARVGC
CCCCCCCHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCC
MPSKLLIAAAEAAHEARHTAAFGVHVDGPVRIDGREVMQRVRSERDRFVGFVVRGVESIP
CCHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDDRLIGRARFIDNTILQVDDHTVVRASRVVIATGSRPAVPPSFGVFGDRLIVNDDVFSW
CCHHHHHHHHHHCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCH
EDLPQSVAVFGPGVIGLELGQALSRLGVRVRVFGVGGGVGPLSDPEIRAYAAKSFNQEFS
HHCCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHH
LLADARVLEMRREGDDAVITFEDADGQTVTERFAYVLAATGRRPNVDTLALGNTALELDG
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCCCCEEEECCEEEEECC
RGVPTFDPVTLQCGESPIFIAGDANNVLPLLHEAADEGKIAGENAALWPKVGPLTRRAPI
CCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
AVVFSDPQIAMVGKRFAELTPGSFVTGEVSFEDQGRSRVMLKNRGLMHVYADRATGRFIG
EEEECCCCEEEHHHHHHHCCCCCEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCEEC
AEWIGPRAENIAHLLAWSYQQGLTIAAMLAMPFYHPVVEEGLRTALRDAQAKLAQLQEAA
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTNQNAQTAQTVQTVQTDVAIIGAGTAGLAAYRAAIAAGKRAVIIEGGAYGTTCARVGC
CCCCCCCHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCC
MPSKLLIAAAEAAHEARHTAAFGVHVDGPVRIDGREVMQRVRSERDRFVGFVVRGVESIP
CCHHHHHHHHHHHHHHHCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
QDDRLIGRARFIDNTILQVDDHTVVRASRVVIATGSRPAVPPSFGVFGDRLIVNDDVFSW
CCHHHHHHHHHHCCEEEEECCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCH
EDLPQSVAVFGPGVIGLELGQALSRLGVRVRVFGVGGGVGPLSDPEIRAYAAKSFNQEFS
HHCCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHH
LLADARVLEMRREGDDAVITFEDADGQTVTERFAYVLAATGRRPNVDTLALGNTALELDG
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHEECCCCCCCCEEEECCEEEEECC
RGVPTFDPVTLQCGESPIFIAGDANNVLPLLHEAADEGKIAGENAALWPKVGPLTRRAPI
CCCCCCCCEEEEECCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCE
AVVFSDPQIAMVGKRFAELTPGSFVTGEVSFEDQGRSRVMLKNRGLMHVYADRATGRFIG
EEEECCCCEEEHHHHHHHCCCCCEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCEEC
AEWIGPRAENIAHLLAWSYQQGLTIAAMLAMPFYHPVVEEGLRTALRDAQAKLAQLQEAA
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]