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The map label for this gene is benA [H]

Identifier: 94313804

GI number: 94313804

Start: 1544108

End: 1545502

Strand: Direct

Name: benA [H]

Synonym: Rmet_4882

Alternate gene names: 94313804

Gene position: 1544108-1545502 (Clockwise)

Preceding gene: 94313803

Following gene: 94313805

Centisome position: 59.85

GC content: 62.94

Gene sequence:

>1395_bases
ATGATCCCGATCCATCTGGATACCGGGGCGGGCCCCGCTCGTCCCCAGCATCAGCCCAACCGCCTGGAGAACCTTGACCA
GTTCCTGGTCGAAGACAAGACCACCGGCGACTATCGGCTGCACCGCAGCGCGTTCACGGACGAGGCGCTGTTCGAACTCG
AGATGCAGCACATCTTCGAGGGCAACTGGATCTACCTGGCGCACGAAAGCCAGATCCCGAACAACAACGATTACTACACG
ACGCATATCGGCCGCCAGCCGATCGTGATCGCGCGCAATCGCCAGGGCGAACTGAACGCGTTCATCAACGCGTGCAGCCA
TCGCGGCGCAATGTTGTGCCGGCACAAGCGCGGCAACAAGGCCACATACACCTGCCCGTTCCACGGCTGGACATTCAACA
ACAGCGGCAAGCTACTCAAGGTCAAGGACCCCGAACAGGCCGGCTATCCGGAATGCTTCAACAAGGAGGGGTCCCACGAC
CTGAAGAAGCTGGCGCGCTTTTCCAACTATCGCGGCTTCCTGTTCGGCAGCCTCAACGCCGATGTGCCGCCGATCGAGCA
GTTCCTGGGCGATGCCGCGCGCATCATCGACATGATTGTCGACCAGTCGCCCGATGGTCTCGAGGTGCTGCGTGGCTCGT
CGACATACACATTCGCCGGCAACTGGAAACTGCAGGCCGAGAATGGCGCCGATGGCTACCATGTGTCCGCCGTGCACTGG
AACTACGCCGCCACCACAAGCCACCGCAAGCAGCAGAACGAACGCGAGGACAAGATCCGCGCGATGGATGCGGGCAAGTG
GGGCCAGCAAGGCGGCGGCTTCTACGCGTTCGATCATGGCCATATGCTGCTGTGGTCGCGCTGGGCTAACCCGGAAGATC
GTCCGAACTTCGCCCGCCGCGACGAGTTCGCGCAACGCTGTGGCGAGGAGCGCGCCGACTGGATGATCCAGAACTCGCGC
AACCTGTGCCTGTACCCCAATGTCTACCTGATGGACCAGTTCGGCTCGCAGATCCGCCTGCTGCGCCCGCTGTCGGTGGA
CCGCACCGAAGTCACGATCTACTGCATCGCCCCCAAGGGGGAATCGGACGAGGCCCGCGCACGCCGCATCCGCCAATACG
AAGACTTTTTCAACGTCAGCGGCATGGCCACGCCCGACGACCTCGAGGAGTTCCGCGCCTGCCAGCAGGGCTACGCGGGC
CAGGCACTGGCCTGGAACGATATGTGCCGGGGCGCCACGCACTGGATCGACGGGCCCGACGACGCAGCGAAGCACATCGG
TCTGAATCCGGTGATGAGCGGGCTGCGTACCGAGGATGAAGGCCTCTATACCGTACAGCATCGCTACTGGCTGGAGACGA
TGAAGCGGGCCGTGGCACAGGGAGATGCAGCATGA

Upstream 100 bases:

>100_bases
CGTGAGATCGCCTGAGACCGCAGAAAGCGATCTGATTCGCCGGGACTCGCCGCCACCGCGCGTCCCGGCCCCTTCCCCGC
CGCTCTGGCAGGAGACCACC

Downstream 100 bases:

>100_bases
GCGCCGTCATCCCCATGAGCGATGTCTCGGCCTTTCTCTACCGCGAGTGCCGCCTGCTCGACGACGAGCAATGGGATGAA
TGGCTGACCTGCTATCACCC

Product: benzoate 1,2-dioxygenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIPIHLDTGAGPARPQHQPNRLENLDQFLVEDKTTGDYRLHRSAFTDEALFELEMQHIFEGNWIYLAHESQIPNNNDYYT
THIGRQPIVIARNRQGELNAFINACSHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEQAGYPECFNKEGSHD
LKKLARFSNYRGFLFGSLNADVPPIEQFLGDAARIIDMIVDQSPDGLEVLRGSSTYTFAGNWKLQAENGADGYHVSAVHW
NYAATTSHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWSRWANPEDRPNFARRDEFAQRCGEERADWMIQNSR
NLCLYPNVYLMDQFGSQIRLLRPLSVDRTEVTIYCIAPKGESDEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAG
QALAWNDMCRGATHWIDGPDDAAKHIGLNPVMSGLRTEDEGLYTVQHRYWLETMKRAVAQGDAA

Sequences:

>Translated_464_residues
MIPIHLDTGAGPARPQHQPNRLENLDQFLVEDKTTGDYRLHRSAFTDEALFELEMQHIFEGNWIYLAHESQIPNNNDYYT
THIGRQPIVIARNRQGELNAFINACSHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEQAGYPECFNKEGSHD
LKKLARFSNYRGFLFGSLNADVPPIEQFLGDAARIIDMIVDQSPDGLEVLRGSSTYTFAGNWKLQAENGADGYHVSAVHW
NYAATTSHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWSRWANPEDRPNFARRDEFAQRCGEERADWMIQNSR
NLCLYPNVYLMDQFGSQIRLLRPLSVDRTEVTIYCIAPKGESDEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAG
QALAWNDMCRGATHWIDGPDDAAKHIGLNPVMSGLRTEDEGLYTVQHRYWLETMKRAVAQGDAA
>Mature_464_residues
MIPIHLDTGAGPARPQHQPNRLENLDQFLVEDKTTGDYRLHRSAFTDEALFELEMQHIFEGNWIYLAHESQIPNNNDYYT
THIGRQPIVIARNRQGELNAFINACSHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEQAGYPECFNKEGSHD
LKKLARFSNYRGFLFGSLNADVPPIEQFLGDAARIIDMIVDQSPDGLEVLRGSSTYTFAGNWKLQAENGADGYHVSAVHW
NYAATTSHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWSRWANPEDRPNFARRDEFAQRCGEERADWMIQNSR
NLCLYPNVYLMDQFGSQIRLLRPLSVDRTEVTIYCIAPKGESDEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAG
QALAWNDMCRGATHWIDGPDDAAKHIGLNPVMSGLRTEDEGLYTVQHRYWLETMKRAVAQGDAA

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=400, Percent_Identity=29.5, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788103, Length=197, Percent_Identity=30.4568527918782, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 53005; Mature: 53005

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIHLDTGAGPARPQHQPNRLENLDQFLVEDKTTGDYRLHRSAFTDEALFELEMQHIFE
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHHHHHCC
GNWIYLAHESQIPNNNDYYTTHIGRQPIVIARNRQGELNAFINACSHRGAMLCRHKRGNK
CCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCC
ATYTCPFHGWTFNNSGKLLKVKDPEQAGYPECFNKEGSHDLKKLARFSNYRGFLFGSLNA
EEEEECCCCEEECCCCCEEEEECCCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEECCCC
DVPPIEQFLGDAARIIDMIVDQSPDGLEVLRGSSTYTFAGNWKLQAENGADGYHVSAVHW
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCEEEEECCEEEEECCCCCCEEEEEEEE
NYAATTSHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWSRWANPEDRPNFARR
CEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHH
DEFAQRCGEERADWMIQNSRNLCLYPNVYLMDQFGSQIRLLRPLSVDRTEVTIYCIAPKG
HHHHHHHCHHHHCEEEECCCCEEECCCEEEECCCCCCEEEEECCCCCCEEEEEEEECCCC
ESDEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAGQALAWNDMCRGATHWIDGPD
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCC
DAAKHIGLNPVMSGLRTEDEGLYTVQHRYWLETMKRAVAQGDAA
HHHHHCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIPIHLDTGAGPARPQHQPNRLENLDQFLVEDKTTGDYRLHRSAFTDEALFELEMQHIFE
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHHHHHHHHCC
GNWIYLAHESQIPNNNDYYTTHIGRQPIVIARNRQGELNAFINACSHRGAMLCRHKRGNK
CCEEEEEECCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCC
ATYTCPFHGWTFNNSGKLLKVKDPEQAGYPECFNKEGSHDLKKLARFSNYRGFLFGSLNA
EEEEECCCCEEECCCCCEEEEECCCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEECCCC
DVPPIEQFLGDAARIIDMIVDQSPDGLEVLRGSSTYTFAGNWKLQAENGADGYHVSAVHW
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHEECCCEEEEECCEEEEECCCCCCEEEEEEEE
NYAATTSHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWSRWANPEDRPNFARR
CEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHH
DEFAQRCGEERADWMIQNSRNLCLYPNVYLMDQFGSQIRLLRPLSVDRTEVTIYCIAPKG
HHHHHHHCHHHHCEEEECCCCEEECCCEEEECCCCCCEEEEECCCCCCEEEEEEEECCCC
ESDEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAGQALAWNDMCRGATHWIDGPD
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCC
DAAKHIGLNPVMSGLRTEDEGLYTVQHRYWLETMKRAVAQGDAA
HHHHHCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]