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The map label for this gene is nodT [H]

Identifier: 94313787

GI number: 94313787

Start: 1523381

End: 1524862

Strand: Reverse

Name: nodT [H]

Synonym: Rmet_4865

Alternate gene names: 94313787

Gene position: 1524862-1523381 (Counterclockwise)

Preceding gene: 94313788

Following gene: 94313786

Centisome position: 59.1

GC content: 68.15

Gene sequence:

>1482_bases
ATGAATAACGCAATGAAGCTGGTCCTGCCGAAGCTGGGTATGCTGGCCGCCGCGCTGGTGCTGGCGGGCTGCTCGCTGGC
GCCGACTTACAAGGTGCCCGAGACGCCCACCGCGTCGACGTTCAAGGAAGCCGAGGCCGCTGCTGCGGAAGGCGCGCAAT
GGAAGACCGCGACGCCGGCCGAAGGCCAGCATCGCGGTGAATGGTGGAAGGTCTTCGGCGACGCCGATCTCGATCGTCTG
ATCGACGCGGCCAACGCCTACAACCAGGACCTCGCTGCCGCCGCGGCGCGTCTGAAGCAGGCACGTGCCTTCACGGGCGC
GACCGAGGCGGACCTGTATCCGCAATTGAGCGCGGGGTTCAGCCCGACGCGCGCGCAGCCCTCGGCAGCCTCGCAAGGCT
TGCCCGATGGCACGCGGGTGTCGCCGCAGACCGTGTACCAGGCGCGTCTGATCGCCAACTACGAGCTTGATCTCTTCGGC
CGCGTGGCCAATAGCGTCAATGCAGCACGCGCCGACGAGCAGGGCGCCGAAGACCTGTATCGTTCGGTGCAGCTCGCGCT
GCAGGCCGATACGGCGCAGGCGTACTTCGCGCTGCGCACGCTTGATAGCGACCGCGATCTGCTCAACGCGACGATCAAGC
TGCGCGAGGACGCGCTGACGCTGCTGCGCAAGCGCTACGAGGCTGGCGAAACCACCGATCTCGATCCGGCCCGCGCCGAG
GCCGAGCTTGGCACCGCCCGCGCCGATCTGGCTGGCATCGAGCGGCGCCGTGCCAACCAGGAGCACGCGCTGGCCGTACT
GACCGGGGTGTCGCCGTCGCAGTTCGCGCTGGCGGCGCGACCGTTTGACACGCCGCCGGTTGCGGTTCCCGCCGGCTTGC
CGTCGGAACTGCTCGAACGTCGCCCCGATATCGCTGCGGCGGAACGCCAGATGGCGGCCGCCAACGCACGGATCGGTGTG
GCAAAGGCGGCGTTCTTCCCACGCATCTCGCTGACAGCGCTGTTCGGCTTCGAGTCGTCGGATATCTCGAACCTGTTCAA
GTGGTCGTCGCGCGCATGGTTGCTTGGGCCGCTGGTGGGTTCCACTGTTGCCCAGACCGTGTTTGACGGTGGCCGCAACA
GTTCGAACCTGGCCGGCGCGCGCGCGGCGCACGAGGAAAGCGTGGCGCGATATCGCCAGACCGTGCTGGTGGCGTTCCGC
GAGGTCGAGGACAGTCTGGCCGACGTACGCTGGTTTAGCCAGCAGGGCGCGGCGCTCGACAGTGCATTGGCCGGCGCGAA
GCGCGCGCAGCGCACTTCGCGCAGCCGCTATGACGCGGGCGCGGTGGACTACCTCACCGTGATCGATGCGGACCGTACGG
TGCTGCAATCGCAACGCGATGCCAATGCGGTGGCCGGGCTGCGGGCGGCGGCAACGGTGTCGCTCGTGCGTAGCCTTGGT
GGCGGTTGGGGCCCTCTGCCGGAGAATGTGGCGAAGAACTGA

Upstream 100 bases:

>100_bases
CGCTGCGTTTGCTGGCCACGCGAGGCCAGCGCCAGAAGGTGGCTGAGCAGCCTGCGTCGGCGTCGCAGGCGGTGGCATCG
GCAGAATAAGGGTGAACAAC

Downstream 100 bases:

>100_bases
GAGCCGTACGCCGCAGGTAACATTTGGCGGCAATTTCACGCTGGCGTGGCGTTTCCGGAGCACTACACTGGAAACGTCGC
GCCAGCTTTCGTTTGGAGGG

Product: RND cation/multidrug efflux system outer membrane protein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNNAMKLVLPKLGMLAAALVLAGCSLAPTYKVPETPTASTFKEAEAAAAEGAQWKTATPAEGQHRGEWWKVFGDADLDRL
IDAANAYNQDLAAAAARLKQARAFTGATEADLYPQLSAGFSPTRAQPSAASQGLPDGTRVSPQTVYQARLIANYELDLFG
RVANSVNAARADEQGAEDLYRSVQLALQADTAQAYFALRTLDSDRDLLNATIKLREDALTLLRKRYEAGETTDLDPARAE
AELGTARADLAGIERRRANQEHALAVLTGVSPSQFALAARPFDTPPVAVPAGLPSELLERRPDIAAAERQMAAANARIGV
AKAAFFPRISLTALFGFESSDISNLFKWSSRAWLLGPLVGSTVAQTVFDGGRNSSNLAGARAAHEESVARYRQTVLVAFR
EVEDSLADVRWFSQQGAALDSALAGAKRAQRTSRSRYDAGAVDYLTVIDADRTVLQSQRDANAVAGLRAAATVSLVRSLG
GGWGPLPENVAKN

Sequences:

>Translated_493_residues
MNNAMKLVLPKLGMLAAALVLAGCSLAPTYKVPETPTASTFKEAEAAAAEGAQWKTATPAEGQHRGEWWKVFGDADLDRL
IDAANAYNQDLAAAAARLKQARAFTGATEADLYPQLSAGFSPTRAQPSAASQGLPDGTRVSPQTVYQARLIANYELDLFG
RVANSVNAARADEQGAEDLYRSVQLALQADTAQAYFALRTLDSDRDLLNATIKLREDALTLLRKRYEAGETTDLDPARAE
AELGTARADLAGIERRRANQEHALAVLTGVSPSQFALAARPFDTPPVAVPAGLPSELLERRPDIAAAERQMAAANARIGV
AKAAFFPRISLTALFGFESSDISNLFKWSSRAWLLGPLVGSTVAQTVFDGGRNSSNLAGARAAHEESVARYRQTVLVAFR
EVEDSLADVRWFSQQGAALDSALAGAKRAQRTSRSRYDAGAVDYLTVIDADRTVLQSQRDANAVAGLRAAATVSLVRSLG
GGWGPLPENVAKN
>Mature_493_residues
MNNAMKLVLPKLGMLAAALVLAGCSLAPTYKVPETPTASTFKEAEAAAAEGAQWKTATPAEGQHRGEWWKVFGDADLDRL
IDAANAYNQDLAAAAARLKQARAFTGATEADLYPQLSAGFSPTRAQPSAASQGLPDGTRVSPQTVYQARLIANYELDLFG
RVANSVNAARADEQGAEDLYRSVQLALQADTAQAYFALRTLDSDRDLLNATIKLREDALTLLRKRYEAGETTDLDPARAE
AELGTARADLAGIERRRANQEHALAVLTGVSPSQFALAARPFDTPPVAVPAGLPSELLERRPDIAAAERQMAAANARIGV
AKAAFFPRISLTALFGFESSDISNLFKWSSRAWLLGPLVGSTVAQTVFDGGRNSSNLAGARAAHEESVARYRQTVLVAFR
EVEDSLADVRWFSQQGAALDSALAGAKRAQRTSRSRYDAGAVDYLTVIDADRTVLQSQRDANAVAGLRAAATVSLVRSLG
GGWGPLPENVAKN

Specific function: The outer membrane component of an organic solvent efflux pump. Involved in export of a number of low log POW compounds including hexane (log POW 3.5), toluene (log POW 2.5) and dimethylphthalate (log POW 2.3). The solvent resistance phenotype has been po

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=31.7894736842105, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI2367348, Length=490, Percent_Identity=25.9183673469388, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52554; Mature: 52554

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAMKLVLPKLGMLAAALVLAGCSLAPTYKVPETPTASTFKEAEAAAAEGAQWKTATPA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EGQHRGEWWKVFGDADLDRLIDAANAYNQDLAAAAARLKQARAFTGATEADLYPQLSAGF
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHCCC
SPTRAQPSAASQGLPDGTRVSPQTVYQARLIANYELDLFGRVANSVNAARADEQGAEDLY
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RSVQLALQADTAQAYFALRTLDSDRDLLNATIKLREDALTLLRKRYEAGETTDLDPARAE
HHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
AELGTARADLAGIERRRANQEHALAVLTGVSPSQFALAARPFDTPPVAVPAGLPSELLER
HHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCHHHEEEECCCCCCCCCCCCCCCHHHHHC
RPDIAAAERQMAAANARIGVAKAAFFPRISLTALFGFESSDISNLFKWSSRAWLLGPLVG
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEHHHHH
STVAQTVFDGGRNSSNLAGARAAHEESVARYRQTVLVAFREVEDSLADVRWFSQQGAALD
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SALAGAKRAQRTSRSRYDAGAVDYLTVIDADRTVLQSQRDANAVAGLRAAATVSLVRSLG
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GGWGPLPENVAKN
CCCCCCCHHHCCC
>Mature Secondary Structure
MNNAMKLVLPKLGMLAAALVLAGCSLAPTYKVPETPTASTFKEAEAAAAEGAQWKTATPA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EGQHRGEWWKVFGDADLDRLIDAANAYNQDLAAAAARLKQARAFTGATEADLYPQLSAGF
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHCCC
SPTRAQPSAASQGLPDGTRVSPQTVYQARLIANYELDLFGRVANSVNAARADEQGAEDLY
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
RSVQLALQADTAQAYFALRTLDSDRDLLNATIKLREDALTLLRKRYEAGETTDLDPARAE
HHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
AELGTARADLAGIERRRANQEHALAVLTGVSPSQFALAARPFDTPPVAVPAGLPSELLER
HHHHHHHHHHHHHHHHHCCCHHEEEEEECCCCHHHEEEECCCCCCCCCCCCCCCHHHHHC
RPDIAAAERQMAAANARIGVAKAAFFPRISLTALFGFESSDISNLFKWSSRAWLLGPLVG
CCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEHHHHH
STVAQTVFDGGRNSSNLAGARAAHEESVARYRQTVLVAFREVEDSLADVRWFSQQGAALD
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
SALAGAKRAQRTSRSRYDAGAVDYLTVIDADRTVLQSQRDANAVAGLRAAATVSLVRSLG
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GGWGPLPENVAKN
CCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9417051; 9852029 [H]