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The map label for this gene is kbl [C]

Identifier: 94313727

GI number: 94313727

Start: 1452342

End: 1453544

Strand: Reverse

Name: kbl [C]

Synonym: Rmet_4805

Alternate gene names: 94313727

Gene position: 1453544-1452342 (Counterclockwise)

Preceding gene: 94313730

Following gene: 94313726

Centisome position: 56.34

GC content: 63.84

Gene sequence:

>1203_bases
ATGTCGAGTGCCGAGGCCTTTTATGCATCCATCCGCGCGGAGCTGGATTCGATCCGCGCCGCGGGCCTGTACAAGCGCGA
GCGCGTCATCGCCACGCCGCAGGGCGCCAAGATCCGCACGGCGGACGGTCGCGAGGTGATCAACCTGTGCGCCAACAACT
ACCTTGGGCTGTCGTCGCACCCCAAGGTCATCGAAGCCGCGCACGAGGCGCTGCGCACGCATGGCTATGGGCTGTCGTCG
GTACGCTTTATCTGCGGCACGCAGGATCTGCACAAGGAACTGGAGGGGCGTCTGGCCCGGTTCCTGGGCACCGAGGACAC
CATCCTCTATGGCTCGGCCTTCGATGCGAACGGTGGGCTGTTCGAGACCATCCTCGGCCCCGAAGACGCCGTGATCAGTG
ATGAACTGAACCACGCGTCGATCATCGACGGCATCCGCCTGTCCAAGGCGCGACGCCTTCGCTACCGGCACAACGATCTG
GACGACCTGCGCGCCCAATTGCGGCAGGCCGATGCGGATGGCGCACGCTTCAAGCTCGTCTTTAGCGATGGCGTGTTCTC
GATGGACGGCACCGTTGCGCGGCTGGACGAGATCCGGAAGATCTGCGACGAGCATGGCGCGCTGCTCGGCATTGACGAAT
GCCACGCCACGGGCTTCATGGGCGCGCGTGGGCGAGGCACGCACGAGGCGCGCGGGGTGTTCGGCAAGATCGACATCATC
ACGGGCACGCTGGGCAAGGCGCTCGGTGGCGCATCGGGCGGCTTTACCTCCGGGCGCAAGGAAGTCGTCGAGCTCTTGCG
CCAGCGTTCCCGACCCTATCTTTTTTCGAATACCGTGGCGCCGGCCATCGTTGGCGGCAGCATTGCCGTGCTCGATATCC
TGGAAGGCAGTACCGAGCTGCGGGACCGGCTGGAGCAGAACACCCAGTTCTTCCGCGAAGGCATCGCGAAGCTTGGCTTC
GACATCAAGCCCGGCGACCATCCGATCGTGCCGATTATGGTCTATGACGCCGAAAAGGCGCAGCAGCTGGCTGACAGACT
GCTGGAACTCGGTGTGTACGTAGTCGGTTTCTTCTATCCCGTGGTCCCGAAGGGACAGGCACGTATCCGCGTGCAGATGA
GCGCTGTGCACGATGCGGTGACGCTGCAGTCCGCTCTTGATGCCTTCGGCCAGGCAGGCCGTGAATTGGGGCTGATTCGA
TGA

Upstream 100 bases:

>100_bases
TCGTCATCTGTTCGTTATAGTGCACGACATGTTCGTTATATAGAACACGTTCGATTTATCGAATTGAGCGTTCCGAGACA
CTACCACCGGGAGTCTGGCC

Downstream 100 bases:

>100_bases
CAGCCGCCACAACAGCCGTGCCGAAAATCCTGATCATTGGCGCAAACGGGCAACTTGGATCGGAGCTGGCTCTGGCATTG
GCGGAACGTCATGGGCGTGA

Product: 2-amino-3-ketobutyrate coenzyme A ligase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSAEAFYASIRAELDSIRAAGLYKRERVIATPQGAKIRTADGREVINLCANNYLGLSSHPKVIEAAHEALRTHGYGLSS
VRFICGTQDLHKELEGRLARFLGTEDTILYGSAFDANGGLFETILGPEDAVISDELNHASIIDGIRLSKARRLRYRHNDL
DDLRAQLRQADADGARFKLVFSDGVFSMDGTVARLDEIRKICDEHGALLGIDECHATGFMGARGRGTHEARGVFGKIDII
TGTLGKALGGASGGFTSGRKEVVELLRQRSRPYLFSNTVAPAIVGGSIAVLDILEGSTELRDRLEQNTQFFREGIAKLGF
DIKPGDHPIVPIMVYDAEKAQQLADRLLELGVYVVGFFYPVVPKGQARIRVQMSAVHDAVTLQSALDAFGQAGRELGLIR

Sequences:

>Translated_400_residues
MSSAEAFYASIRAELDSIRAAGLYKRERVIATPQGAKIRTADGREVINLCANNYLGLSSHPKVIEAAHEALRTHGYGLSS
VRFICGTQDLHKELEGRLARFLGTEDTILYGSAFDANGGLFETILGPEDAVISDELNHASIIDGIRLSKARRLRYRHNDL
DDLRAQLRQADADGARFKLVFSDGVFSMDGTVARLDEIRKICDEHGALLGIDECHATGFMGARGRGTHEARGVFGKIDII
TGTLGKALGGASGGFTSGRKEVVELLRQRSRPYLFSNTVAPAIVGGSIAVLDILEGSTELRDRLEQNTQFFREGIAKLGF
DIKPGDHPIVPIMVYDAEKAQQLADRLLELGVYVVGFFYPVVPKGQARIRVQMSAVHDAVTLQSALDAFGQAGRELGLIR
>Mature_399_residues
SSAEAFYASIRAELDSIRAAGLYKRERVIATPQGAKIRTADGREVINLCANNYLGLSSHPKVIEAAHEALRTHGYGLSSV
RFICGTQDLHKELEGRLARFLGTEDTILYGSAFDANGGLFETILGPEDAVISDELNHASIIDGIRLSKARRLRYRHNDLD
DLRAQLRQADADGARFKLVFSDGVFSMDGTVARLDEIRKICDEHGALLGIDECHATGFMGARGRGTHEARGVFGKIDIIT
GTLGKALGGASGGFTSGRKEVVELLRQRSRPYLFSNTVAPAIVGGSIAVLDILEGSTELRDRLEQNTQFFREGIAKLGFD
IKPGDHPIVPIMVYDAEKAQQLADRLLELGVYVVGFFYPVVPKGQARIRVQMSAVHDAVTLQSALDAFGQAGRELGLIR

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=386, Percent_Identity=55.699481865285, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI284448556, Length=412, Percent_Identity=52.1844660194175, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI83977440, Length=360, Percent_Identity=34.7222222222222, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI83977444, Length=360, Percent_Identity=34.7222222222222, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI83977442, Length=360, Percent_Identity=34.7222222222222, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI4502025, Length=360, Percent_Identity=33.3333333333333, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI40316939, Length=360, Percent_Identity=33.3333333333333, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI4758668, Length=371, Percent_Identity=33.9622641509434, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI119220554, Length=373, Percent_Identity=34.0482573726542, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI5454084, Length=276, Percent_Identity=30.0724637681159, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1790046, Length=393, Percent_Identity=58.7786259541985, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1786993, Length=349, Percent_Identity=33.810888252149, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71994529, Length=401, Percent_Identity=55.860349127182, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI32566772, Length=374, Percent_Identity=31.5508021390374, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17560914, Length=367, Percent_Identity=30.2452316076294, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17560912, Length=367, Percent_Identity=30.2452316076294, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71982617, Length=288, Percent_Identity=26.0416666666667, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71982625, Length=288, Percent_Identity=26.0416666666667, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320438, Length=394, Percent_Identity=30.7106598984772, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320267, Length=369, Percent_Identity=30.6233062330623, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323954, Length=282, Percent_Identity=28.3687943262411, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24662918, Length=389, Percent_Identity=55.5269922879177, Blast_Score=449, Evalue=1e-126,
Organism=Drosophila melanogaster, GI17137420, Length=339, Percent_Identity=35.3982300884956, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17136286, Length=371, Percent_Identity=34.5013477088949, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24653280, Length=387, Percent_Identity=25.3229974160207, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653276, Length=387, Percent_Identity=25.3229974160207, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24653278, Length=387, Percent_Identity=25.3229974160207, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011282
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 43524; Mature: 43393

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSAEAFYASIRAELDSIRAAGLYKRERVIATPQGAKIRTADGREVINLCANNYLGLSSH
CCCHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCC
PKVIEAAHEALRTHGYGLSSVRFICGTQDLHKELEGRLARFLGTEDTILYGSAFDANGGL
CHHHHHHHHHHHHCCCCHHHHHEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCE
FETILGPEDAVISDELNHASIIDGIRLSKARRLRYRHNDLDDLRAQLRQADADGARFKLV
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEE
FSDGVFSMDGTVARLDEIRKICDEHGALLGIDECHATGFMGARGRGTHEARGVFGKIDII
EECCEEECCCHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCCCHHHCCCEEEEEEE
TGTLGKALGGASGGFTSGRKEVVELLRQRSRPYLFSNTVAPAIVGGSIAVLDILEGSTEL
ECHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEEEEECCCCHHH
RDRLEQNTQFFREGIAKLGFDIKPGDHPIVPIMVYDAEKAQQLADRLLELGVYVVGFFYP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VVPKGQARIRVQMSAVHDAVTLQSALDAFGQAGRELGLIR
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SSAEAFYASIRAELDSIRAAGLYKRERVIATPQGAKIRTADGREVINLCANNYLGLSSH
CCHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCCEEEECCCHHHHHHHHCCCCCCCCC
PKVIEAAHEALRTHGYGLSSVRFICGTQDLHKELEGRLARFLGTEDTILYGSAFDANGGL
CHHHHHHHHHHHHCCCCHHHHHEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCE
FETILGPEDAVISDELNHASIIDGIRLSKARRLRYRHNDLDDLRAQLRQADADGARFKLV
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEE
FSDGVFSMDGTVARLDEIRKICDEHGALLGIDECHATGFMGARGRGTHEARGVFGKIDII
EECCEEECCCHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCCCHHHCCCEEEEEEE
TGTLGKALGGASGGFTSGRKEVVELLRQRSRPYLFSNTVAPAIVGGSIAVLDILEGSTEL
ECHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHCCCCEEEEEECCCCHHH
RDRLEQNTQFFREGIAKLGFDIKPGDHPIVPIMVYDAEKAQQLADRLLELGVYVVGFFYP
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VVPKGQARIRVQMSAVHDAVTLQSALDAFGQAGRELGLIR
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]