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The map label for this gene is vapI [H]

Identifier: 94313686

GI number: 94313686

Start: 1408516

End: 1408863

Strand: Direct

Name: vapI [H]

Synonym: Rmet_4764

Alternate gene names: 94313686

Gene position: 1408516-1408863 (Clockwise)

Preceding gene: 94313685

Following gene: 94313687

Centisome position: 54.59

GC content: 63.79

Gene sequence:

>348_bases
ATGACAATCAACCGCGAAGACTTGCGGCGCGCGGATTTTCGCAGCATTGCCACCGGCCAGCCGATTCCGCCGGCACACCC
CGGCGATGTACTGGAAACCGAGTTCATGGCGCCAAACGAACTGTCGGCGGAAGCATTGGCGGTGGCCTTGCATATGGCGC
CGCAACGAATTCACGATATCGTGCGGCGTCAGCGAGCCATCTCGCCAGACACCGCCCTGCGGCTGGCCCGCTATTTCGGC
ACTAGTGCGGAATTCTGGGCGGGCCTCCAGACCGCATATGCACTGCGGCTGGCGCGCGCGACTGCAGGGGCGAACATCGA
GCGCGACATCCAGCCGCTGACAGCATGA

Upstream 100 bases:

>100_bases
GCGCGTCCGCATTAAAGGTCCGTGGCATCTCTGTTTTCGCTTCATCAACGGCGAAGCCTGGGACGTCGGCATCGCCGAAT
ACGAGGAATGAGGAGCGCGC

Downstream 100 bases:

>100_bases
CCTGCCCTTGCGCCACCACGACAGTGATGCTTACGCCACCAAGGCCGTACCCCGTCACATGCCGATGGTATGCTCTGACT
TTTCAATTCCCGCGCGCACA

Product: plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 115; Mature: 114

Protein sequence:

>115_residues
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQTAYALRLARATAGANIERDIQPLTA

Sequences:

>Translated_115_residues
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFG
TSAEFWAGLQTAYALRLARATAGANIERDIQPLTA
>Mature_114_residues
TINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDIVRRQRAISPDTALRLARYFGT
SAEFWAGLQTAYALRLARATAGANIERDIQPLTA

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 12692; Mature: 12560

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDI
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCHHHHHHH
VRRQRAISPDTALRLARYFGTSAEFWAGLQTAYALRLARATAGANIERDIQPLTA
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TINREDLRRADFRSIATGQPIPPAHPGDVLETEFMAPNELSAEALAVALHMAPQRIHDI
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHCHHHHHHH
VRRQRAISPDTALRLARYFGTSAEFWAGLQTAYALRLARATAGANIERDIQPLTA
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]