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The map label for this gene is bcr [H]

Identifier: 94313674

GI number: 94313674

Start: 1396308

End: 1397588

Strand: Reverse

Name: bcr [H]

Synonym: Rmet_4752

Alternate gene names: 94313674

Gene position: 1397588-1396308 (Counterclockwise)

Preceding gene: 94313676

Following gene: 291481541

Centisome position: 54.17

GC content: 64.87

Gene sequence:

>1281_bases
GTGCGCATTTGCCGGCTGTGCAACAATTGCGCCTTGCGTTTCCGGAACCCTTTCATGACGTCGACCAAGGGCGCGCCATC
GTCCTCCCGTTTTCCCCTCTGGCTTCTGATTTGCGCATCGCTGACCGCCATCGCGCCGCTGTCCATCGATATGTACCTGC
CGAGCTTCCCGGCGTTGGCAGCAGATCTGCATACCGACATCGGACAGGTGCAGCTGACGCTTGGCACGTTTCTGGTTGGG
CTGGCGATTGGACAGGCGTTTTACGGCCCGATCAGCGATCGCTTCGGCCGCAAGCCGCCACTGTACGTCGGACTCACGAT
CTACGGCGCGGCGGCTGCAGGCTGTGCGATGGCGTCGACCGTCGATTCGTTGATGCTCTGGCGGTTCCTGCAGGCGCTTG
GCGGCTGTACCGGCCTGGTAGTCTCGCGCGCGGTGATTCGTGACCGGATGGAAGCCCATGAGTCGGCGCGGGCGTACTCC
TCGCTGATGCTCGTGATGGGCCTGGCGCCGATCCTGGCGCCGCTGATTGGTGGCGCGGTGCTGGCGGCTTCCGGCTGGCG
TGCGATCTTCTGGATTCACTGTGGCGCAGCGGTGCTGCTGCTGTGGTTCGTCCACGTACGCATGGAGGAGTCGCTCGACG
TGCAGCGCCGCCGGACACTGCATCCGGTTGCAGTGCTGCGCAGCTACTGGGAGTTGCTGCGCGATCGCGAATTTCTCGGC
TATTCGCTGGCGGCGGGATGCGTGCAGGGCGGCATGTTCGCCTATATTGCGGGGTCGCCGTTTGTGCTGATCGAACTGCA
TGGCATCGATCCCTCCCACTACGGCTTTGTTTTCGGGGCGAATGCGTTTGGCCTGATCGGCATGTCGCAGGTCAATACCC
GCCTGGTGCGGGCGCGCGCGCTTGACGATGTGTTGCGCGTGGCGCTGTTCGCGCCTTGCGTGGCAGGTCTCGTCCTGGCC
GGCACAGCCCTGATCGGCTGGGCAACGCTTCCGGTACTGCTGGTCTGCTTCTTCGCATTCCTGTCGGGCCTTGGCTGCAT
CACGCCCAACGCCTCGGCTTTGGCGCTATCCCATCAGAGTCATCGCGCCGGCACGGCTTCGGCGTTGATGGGCACGTTGC
AGTTCGGCTTCGGCACGCTGGCAGGCGCGGCGGTCAGCCTCTGGCACGATGGCACCGCGTTGCCGCTGGCCGTTGTCATG
GCTGTCTGCGGCAGTGGCGCGGTCGTGCTGCGTCTCTACGGGCGGGCAGGTATGCGGCAGCGCTCCCAATCCGCAACCTG
A

Upstream 100 bases:

>100_bases
ACCCCGCCGCGCGTGAAAGCCAGTCCAACAATGTTCCGCACCGGTGGCGGGTTGCCTGGCGATCGATGCCAACATCCTTG
CTTCCCCGACGGGTGGGCCG

Downstream 100 bases:

>100_bases
TCCCGCCGAACTTGCCTGGCCGGATCAAGCTCAGGCCGCCTGGTTTGCCGGCGTGCCCGATGCTACCGATACGGCCGGCG
CGTTCTGCATCACCACGACC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRICRLCNNCALRFRNPFMTSTKGAPSSSRFPLWLLICASLTAIAPLSIDMYLPSFPALAADLHTDIGQVQLTLGTFLVG
LAIGQAFYGPISDRFGRKPPLYVGLTIYGAAAAGCAMASTVDSLMLWRFLQALGGCTGLVVSRAVIRDRMEAHESARAYS
SLMLVMGLAPILAPLIGGAVLAASGWRAIFWIHCGAAVLLLWFVHVRMEESLDVQRRRTLHPVAVLRSYWELLRDREFLG
YSLAAGCVQGGMFAYIAGSPFVLIELHGIDPSHYGFVFGANAFGLIGMSQVNTRLVRARALDDVLRVALFAPCVAGLVLA
GTALIGWATLPVLLVCFFAFLSGLGCITPNASALALSHQSHRAGTASALMGTLQFGFGTLAGAAVSLWHDGTALPLAVVM
AVCGSGAVVLRLYGRAGMRQRSQSAT

Sequences:

>Translated_426_residues
MRICRLCNNCALRFRNPFMTSTKGAPSSSRFPLWLLICASLTAIAPLSIDMYLPSFPALAADLHTDIGQVQLTLGTFLVG
LAIGQAFYGPISDRFGRKPPLYVGLTIYGAAAAGCAMASTVDSLMLWRFLQALGGCTGLVVSRAVIRDRMEAHESARAYS
SLMLVMGLAPILAPLIGGAVLAASGWRAIFWIHCGAAVLLLWFVHVRMEESLDVQRRRTLHPVAVLRSYWELLRDREFLG
YSLAAGCVQGGMFAYIAGSPFVLIELHGIDPSHYGFVFGANAFGLIGMSQVNTRLVRARALDDVLRVALFAPCVAGLVLA
GTALIGWATLPVLLVCFFAFLSGLGCITPNASALALSHQSHRAGTASALMGTLQFGFGTLAGAAVSLWHDGTALPLAVVM
AVCGSGAVVLRLYGRAGMRQRSQSAT
>Mature_426_residues
MRICRLCNNCALRFRNPFMTSTKGAPSSSRFPLWLLICASLTAIAPLSIDMYLPSFPALAADLHTDIGQVQLTLGTFLVG
LAIGQAFYGPISDRFGRKPPLYVGLTIYGAAAAGCAMASTVDSLMLWRFLQALGGCTGLVVSRAVIRDRMEAHESARAYS
SLMLVMGLAPILAPLIGGAVLAASGWRAIFWIHCGAAVLLLWFVHVRMEESLDVQRRRTLHPVAVLRSYWELLRDREFLG
YSLAAGCVQGGMFAYIAGSPFVLIELHGIDPSHYGFVFGANAFGLIGMSQVNTRLVRARALDDVLRVALFAPCVAGLVLA
GTALIGWATLPVLLVCFFAFLSGLGCITPNASALALSHQSHRAGTASALMGTLQFGFGTLAGAAVSLWHDGTALPLAVVM
AVCGSGAVVLRLYGRAGMRQRSQSAT

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=382, Percent_Identity=35.8638743455497, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI48994889, Length=399, Percent_Identity=32.5814536340852, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1790146, Length=351, Percent_Identity=28.2051282051282, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87082312, Length=349, Percent_Identity=30.3724928366762, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787065, Length=371, Percent_Identity=25.3369272237197, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790794, Length=394, Percent_Identity=23.6040609137056, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324264, Length=157, Percent_Identity=28.0254777070064, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319480, Length=385, Percent_Identity=22.3376623376623, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45326; Mature: 45326

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRICRLCNNCALRFRNPFMTSTKGAPSSSRFPLWLLICASLTAIAPLSIDMYLPSFPALA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHH
ADLHTDIGQVQLTLGTFLVGLAIGQAFYGPISDRFGRKPPLYVGLTIYGAAAAGCAMAST
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEHHHHHHHHHHHHH
VDSLMLWRFLQALGGCTGLVVSRAVIRDRMEAHESARAYSSLMLVMGLAPILAPLIGGAV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LAASGWRAIFWIHCGAAVLLLWFVHVRMEESLDVQRRRTLHPVAVLRSYWELLRDREFLG
HHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCHHHH
YSLAAGCVQGGMFAYIAGSPFVLIELHGIDPSHYGFVFGANAFGLIGMSQVNTRLVRARA
HHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCEEEECCCHHHCCHHHHHHHHHHHHH
LDDVLRVALFAPCVAGLVLAGTALIGWATLPVLLVCFFAFLSGLGCITPNASALALSHQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
HRAGTASALMGTLQFGFGTLAGAAVSLWHDGTALPLAVVMAVCGSGAVVLRLYGRAGMRQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHCHHH
RSQSAT
HCCCCC
>Mature Secondary Structure
MRICRLCNNCALRFRNPFMTSTKGAPSSSRFPLWLLICASLTAIAPLSIDMYLPSFPALA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHH
ADLHTDIGQVQLTLGTFLVGLAIGQAFYGPISDRFGRKPPLYVGLTIYGAAAAGCAMAST
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEHHHHHHHHHHHHH
VDSLMLWRFLQALGGCTGLVVSRAVIRDRMEAHESARAYSSLMLVMGLAPILAPLIGGAV
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LAASGWRAIFWIHCGAAVLLLWFVHVRMEESLDVQRRRTLHPVAVLRSYWELLRDREFLG
HHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHCCHHHH
YSLAAGCVQGGMFAYIAGSPFVLIELHGIDPSHYGFVFGANAFGLIGMSQVNTRLVRARA
HHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCEEEECCCHHHCCHHHHHHHHHHHHH
LDDVLRVALFAPCVAGLVLAGTALIGWATLPVLLVCFFAFLSGLGCITPNASALALSHQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
HRAGTASALMGTLQFGFGTLAGAAVSLWHDGTALPLAVVMAVCGSGAVVLRLYGRAGMRQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEHHHHCHHH
RSQSAT
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]