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The map label for this gene is cls [H]

Identifier: 94313646

GI number: 94313646

Start: 1366191

End: 1367708

Strand: Reverse

Name: cls [H]

Synonym: Rmet_4724

Alternate gene names: 94313646

Gene position: 1367708-1366191 (Counterclockwise)

Preceding gene: 94313648

Following gene: 94313645

Centisome position: 53.01

GC content: 63.7

Gene sequence:

>1518_bases
ATGGATTCGGGTGCGAGGCTTGGGGATGTTGGAAGTGATGTGCTGTGCCAGAATAGCGTCCATCCCGTCCGGCCCCGGCT
GCCTATGTTGACCCCTAGTGTGATTGCAGCGATCGTCGTGTCTCTTCACGTGCTGGGCGTGATTGCGGCGCTGCACGCCG
TCATGACCGTACGCACTGCGCCCGGGGCCATAGCCTGGGCTGGATCGCTGGTCATGATGCCGTACTTCTCGCTGATCCCC
TATCTGATCTTTGGACGAAGCCGCTTCGCGGGATACGTCAATGCGCGGCGCTTCAACAATCACCGTCTGCGCGAGATCCG
CCAGAACATGAGTACCCAGGAGCTGGATTCGCTCAGCGCCTGCCTGGTGATGAACCCCGACCAGGAATGCATGCGCGCAT
TGCCGCGTCTGACTGGCATGCCATGCCTGGGTAATAACGACGTGCGGCTGCTGGTCAACGGCGAGGCGACCTTCCAGGCG
ATCTTCGCCGCGATCGATGCCGCCACGCAGGTCGTGGTGGTCCAGTTCTTCATCGTTCATGATGATGAGCTTGGACTCGA
ACTGCAACGCCGCCTGTGCGCGCGTGCCAAGGCGGGGGTCAGGGTCTATTTCCTATACGACAGCATCGGCTGTCACGCGC
TAGGCCGGGCCTACCTGCGTGCGCTGGAGGAAGCCGGCGTCCACGTGCGCTCATTCGCCACGCATCCCGGCTTTGTCAAT
CGCTTCCAGCTCAACTTCCGCAATCACCGCAAGCTGGTCATCGTCGATGGCGAGCGCGCCTTTGTCGGGGGTCATAACGT
CGGCAACGAGTACCTCGGTCGCCGGCCACCACTCGCGCCCTGGCGGGATACCCATATCGAGATTCGCGGCGCGGTGGTGC
TCGAGCTGCAGATGACGTTCGCCGAGGACTGGTACTGGGCCGCGCACGAGGTGCCCTACCTGTTGCTGCCACCGCCGAGG
CGTTTCGGCGACATGGTGTGCCAGATCGTGCCATCCGGGCCGGCGGACGCGCAGGAGACATGCTCGCTGTTCTTCGTCGA
GGCGATCCAGGCCGCGCGCAAGCGGATCTGGATTACGTCGCCATACTTCGTGCCCGACGAAGCCGTTTCCGCGATGCTAC
GGCTGGCAGTGCTGCGTGGTGTGGACGTGCGCATCCTGATACCGGCCCGGCCGGACCATTACGTGGTCTATGGGGCGTCG
ACGATCTATGCCCACCAGGCCGTCTGCGCCGGCGTGAAGGTGTTCCGCTACCAGCCGGGCTTCCTGCACCAGAAGGTCAT
CCTGGTCGACGACGACGCGGCGGCTGTCGGCACCGCGAATCTGGACAATCGCTCGTTCCGGCTCAACTTCGAGATGATGG
TCATGACGGCTCACCCGGCATTCGCGGCCAGCGTGGCCGAGATGCTGGAAGCCGATTTCGCGCAGGCCCGTCAGATCGAC
CGCGAGGACTTCCTTGGCGCGCCCGCGATCCTGCGCGTGGCGATGCACGTGGCCAAGCTCTTCGCTCCCATCCTTTAG

Upstream 100 bases:

>100_bases
GGTGTCGCTTATCCACAGATTGTGTGGATAACTGCGTCGGCGCCTCCGCCAGTCGCGCCGGGTCATGGGAGCGCATCGGA
CTGCCTAAAAATTAAGCAGC

Downstream 100 bases:

>100_bases
TGACGCGGGAAATACGCGAACCGCGCGGACGAACGGCGCTGGAAGTGTTTGTGTACACTGTCTACTATTGTTATTCGTTG
CCCTGGACGTTGTCCGCATG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MDSGARLGDVGSDVLCQNSVHPVRPRLPMLTPSVIAAIVVSLHVLGVIAALHAVMTVRTAPGAIAWAGSLVMMPYFSLIP
YLIFGRSRFAGYVNARRFNNHRLREIRQNMSTQELDSLSACLVMNPDQECMRALPRLTGMPCLGNNDVRLLVNGEATFQA
IFAAIDAATQVVVVQFFIVHDDELGLELQRRLCARAKAGVRVYFLYDSIGCHALGRAYLRALEEAGVHVRSFATHPGFVN
RFQLNFRNHRKLVIVDGERAFVGGHNVGNEYLGRRPPLAPWRDTHIEIRGAVVLELQMTFAEDWYWAAHEVPYLLLPPPR
RFGDMVCQIVPSGPADAQETCSLFFVEAIQAARKRIWITSPYFVPDEAVSAMLRLAVLRGVDVRILIPARPDHYVVYGAS
TIYAHQAVCAGVKVFRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFEMMVMTAHPAFAASVAEMLEADFAQARQID
REDFLGAPAILRVAMHVAKLFAPIL

Sequences:

>Translated_505_residues
MDSGARLGDVGSDVLCQNSVHPVRPRLPMLTPSVIAAIVVSLHVLGVIAALHAVMTVRTAPGAIAWAGSLVMMPYFSLIP
YLIFGRSRFAGYVNARRFNNHRLREIRQNMSTQELDSLSACLVMNPDQECMRALPRLTGMPCLGNNDVRLLVNGEATFQA
IFAAIDAATQVVVVQFFIVHDDELGLELQRRLCARAKAGVRVYFLYDSIGCHALGRAYLRALEEAGVHVRSFATHPGFVN
RFQLNFRNHRKLVIVDGERAFVGGHNVGNEYLGRRPPLAPWRDTHIEIRGAVVLELQMTFAEDWYWAAHEVPYLLLPPPR
RFGDMVCQIVPSGPADAQETCSLFFVEAIQAARKRIWITSPYFVPDEAVSAMLRLAVLRGVDVRILIPARPDHYVVYGAS
TIYAHQAVCAGVKVFRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFEMMVMTAHPAFAASVAEMLEADFAQARQID
REDFLGAPAILRVAMHVAKLFAPIL
>Mature_505_residues
MDSGARLGDVGSDVLCQNSVHPVRPRLPMLTPSVIAAIVVSLHVLGVIAALHAVMTVRTAPGAIAWAGSLVMMPYFSLIP
YLIFGRSRFAGYVNARRFNNHRLREIRQNMSTQELDSLSACLVMNPDQECMRALPRLTGMPCLGNNDVRLLVNGEATFQA
IFAAIDAATQVVVVQFFIVHDDELGLELQRRLCARAKAGVRVYFLYDSIGCHALGRAYLRALEEAGVHVRSFATHPGFVN
RFQLNFRNHRKLVIVDGERAFVGGHNVGNEYLGRRPPLAPWRDTHIEIRGAVVLELQMTFAEDWYWAAHEVPYLLLPPPR
RFGDMVCQIVPSGPADAQETCSLFFVEAIQAARKRIWITSPYFVPDEAVSAMLRLAVLRGVDVRILIPARPDHYVVYGAS
TIYAHQAVCAGVKVFRYQPGFLHQKVILVDDDAAAVGTANLDNRSFRLNFEMMVMTAHPAFAASVAEMLEADFAQARQID
REDFLGAPAILRVAMHVAKLFAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=495, Percent_Identity=26.6666666666667, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1787007, Length=346, Percent_Identity=29.7687861271676, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 56054; Mature: 56054

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGARLGDVGSDVLCQNSVHPVRPRLPMLTPSVIAAIVVSLHVLGVIAALHAVMTVRTA
CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGAIAWAGSLVMMPYFSLIPYLIFGRSRFAGYVNARRFNNHRLREIRQNMSTQELDSLSA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCCE
CLVMNPDQECMRALPRLTGMPCLGNNDVRLLVNGEATFQAIFAAIDAATQVVVVQFFIVH
EEEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEE
DDELGLELQRRLCARAKAGVRVYFLYDSIGCHALGRAYLRALEEAGVHVRSFATHPGFVN
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
RFQLNFRNHRKLVIVDGERAFVGGHNVGNEYLGRRPPLAPWRDTHIEIRGAVVLELQMTF
EEEEEECCCCEEEEECCCEEEECCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEEH
AEDWYWAAHEVPYLLLPPPRRFGDMVCQIVPSGPADAQETCSLFFVEAIQAARKRIWITS
HCCCEEHHHCCCEEEECCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEEC
PYFVPDEAVSAMLRLAVLRGVDVRILIPARPDHYVVYGASTIYAHQAVCAGVKVFRYQPG
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHCCEEEEECCC
FLHQKVILVDDDAAAVGTANLDNRSFRLNFEMMVMTAHPAFAASVAEMLEADFAQARQID
CCEEEEEEEECCCCEEECCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCC
REDFLGAPAILRVAMHVAKLFAPIL
HHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSGARLGDVGSDVLCQNSVHPVRPRLPMLTPSVIAAIVVSLHVLGVIAALHAVMTVRTA
CCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGAIAWAGSLVMMPYFSLIPYLIFGRSRFAGYVNARRFNNHRLREIRQNMSTQELDSLSA
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCCCE
CLVMNPDQECMRALPRLTGMPCLGNNDVRLLVNGEATFQAIFAAIDAATQVVVVQFFIVH
EEEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEE
DDELGLELQRRLCARAKAGVRVYFLYDSIGCHALGRAYLRALEEAGVHVRSFATHPGFVN
CCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCE
RFQLNFRNHRKLVIVDGERAFVGGHNVGNEYLGRRPPLAPWRDTHIEIRGAVVLELQMTF
EEEEEECCCCEEEEECCCEEEECCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEEH
AEDWYWAAHEVPYLLLPPPRRFGDMVCQIVPSGPADAQETCSLFFVEAIQAARKRIWITS
HCCCEEHHHCCCEEEECCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEEC
PYFVPDEAVSAMLRLAVLRGVDVRILIPARPDHYVVYGASTIYAHQAVCAGVKVFRYQPG
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHCCEEEEECCC
FLHQKVILVDDDAAAVGTANLDNRSFRLNFEMMVMTAHPAFAASVAEMLEADFAQARQID
CCEEEEEEEECCCCEEECCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCC
REDFLGAPAILRVAMHVAKLFAPIL
HHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA