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The map label for this gene is speE [H]

Identifier: 94313585

GI number: 94313585

Start: 1296546

End: 1297361

Strand: Reverse

Name: speE [H]

Synonym: Rmet_4663

Alternate gene names: 94313585

Gene position: 1297361-1296546 (Counterclockwise)

Preceding gene: 94313586

Following gene: 94313584

Centisome position: 50.28

GC content: 57.48

Gene sequence:

>816_bases
ATGAATTCCCAACGCGCCTCTTTGAGAGAGTACCTACGGTTTCTAGCTACAGTCACCAACGATGGGAAGCCTGTAGTACT
GGAGACGGAGCAGGCACTTTCGCTCCATTTCGACATACACGCGACGCAGAGCCTCATGTCTGTGCGCGACCCCAACAAGC
TCGTGCTCGGCTATACACGCACGATGATGAGTTTTCTGCTGCTGCAGCCGACGCCGTCACGTATCTCCATGATCGGCCTA
GGTGGGGGATCCCTTGCCAAATACTGCTACCACCATCTTCCCAGTGCGAAGGTGGTGGCAATTGAGATTGACCCGGCAGT
GATTGCCTTGCGCGACAAGTTCCAGATTCCGCAGGATGACGACCGGTTCGAAGTCATCTGCGCGGATGGCGCGGAGTATG
TCAAGGGACAGGATGCTCGACCCGATGTCATTCTGGTGGACGGGTTTCTGGCGCACGGCATGCCTGCACAACTCGGAACA
ATCGCCTTCTACTCGGCCTGCCATGCGCGGCTTGCCGACGATGGCGTCCTCGTCGCCAATTTCTTGGAGAACGACAGGGA
CATCCCCTTCTATCTCGAAGAGATTCGCTCCGTATTCGGTGATTCGTTTTCCGTGTCGCTGACCGAAGATAGCTGTAATT
ACACGTTGATCGCGTGGAAGGGAACATGGGAGCTCCCTTCGCTGGATACGCTGGTCACGCGTGCGCGCCAGCTCGAGACC
TCGCACCCCTTGAACCTGCGCTCGGCCGCCAGCCGTCTGAAGCGTGGCAGACACCTCGCGTCTGACCATACGTACTGGCA
GAGGCTGACAAGATAG

Upstream 100 bases:

>100_bases
TACGGGTTCAGGCGAGTGGCTCGGATGAGGCCGCCGCCGTGAAGCGGTTGGTGGCGTTACTGACAGCCTGAACCACCCAC
AAGTACTGACCGAGTATCAG

Downstream 100 bases:

>100_bases
GCGGCGCCAGCCCACGATGCCCCCACATTTGCCCAAGCGCCTTTCCAAGTAAGGGGAGCATGCTGATCCCATACCTGCGC
CGACTGACGGGGAAGGACCG

Product: putative spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNSQRASLREYLRFLATVTNDGKPVVLETEQALSLHFDIHATQSLMSVRDPNKLVLGYTRTMMSFLLLQPTPSRISMIGL
GGGSLAKYCYHHLPSAKVVAIEIDPAVIALRDKFQIPQDDDRFEVICADGAEYVKGQDARPDVILVDGFLAHGMPAQLGT
IAFYSACHARLADDGVLVANFLENDRDIPFYLEEIRSVFGDSFSVSLTEDSCNYTLIAWKGTWELPSLDTLVTRARQLET
SHPLNLRSAASRLKRGRHLASDHTYWQRLTR

Sequences:

>Translated_271_residues
MNSQRASLREYLRFLATVTNDGKPVVLETEQALSLHFDIHATQSLMSVRDPNKLVLGYTRTMMSFLLLQPTPSRISMIGL
GGGSLAKYCYHHLPSAKVVAIEIDPAVIALRDKFQIPQDDDRFEVICADGAEYVKGQDARPDVILVDGFLAHGMPAQLGT
IAFYSACHARLADDGVLVANFLENDRDIPFYLEEIRSVFGDSFSVSLTEDSCNYTLIAWKGTWELPSLDTLVTRARQLET
SHPLNLRSAASRLKRGRHLASDHTYWQRLTR
>Mature_271_residues
MNSQRASLREYLRFLATVTNDGKPVVLETEQALSLHFDIHATQSLMSVRDPNKLVLGYTRTMMSFLLLQPTPSRISMIGL
GGGSLAKYCYHHLPSAKVVAIEIDPAVIALRDKFQIPQDDDRFEVICADGAEYVKGQDARPDVILVDGFLAHGMPAQLGT
IAFYSACHARLADDGVLVANFLENDRDIPFYLEEIRSVFGDSFSVSLTEDSCNYTLIAWKGTWELPSLDTLVTRARQLET
SHPLNLRSAASRLKRGRHLASDHTYWQRLTR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 30356; Mature: 30356

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQRASLREYLRFLATVTNDGKPVVLETEQALSLHFDIHATQSLMSVRDPNKLVLGYTR
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEHHHHHHHHHHCCCCEEEEHHHH
TMMSFLLLQPTPSRISMIGLGGGSLAKYCYHHLPSAKVVAIEIDPAVIALRDKFQIPQDD
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCC
DRFEVICADGAEYVKGQDARPDVILVDGFLAHGMPAQLGTIAFYSACHARLADDGVLVAN
CCEEEEECCCHHHHCCCCCCCCEEEEECHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
FLENDRDIPFYLEEIRSVFGDSFSVSLTEDSCNYTLIAWKGTWELPSLDTLVTRARQLET
ECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCC
SHPLNLRSAASRLKRGRHLASDHTYWQRLTR
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MNSQRASLREYLRFLATVTNDGKPVVLETEQALSLHFDIHATQSLMSVRDPNKLVLGYTR
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEHHHHHHHHHHCCCCEEEEHHHH
TMMSFLLLQPTPSRISMIGLGGGSLAKYCYHHLPSAKVVAIEIDPAVIALRDKFQIPQDD
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCC
DRFEVICADGAEYVKGQDARPDVILVDGFLAHGMPAQLGTIAFYSACHARLADDGVLVAN
CCEEEEECCCHHHHCCCCCCCCEEEEECHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
FLENDRDIPFYLEEIRSVFGDSFSVSLTEDSCNYTLIAWKGTWELPSLDTLVTRARQLET
ECCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCC
SHPLNLRSAASRLKRGRHLASDHTYWQRLTR
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]