| Definition | Cupriavidus metallidurans CH34 megaplasmid, complete sequence. |
|---|---|
| Accession | NC_007974 |
| Length | 2,580,084 |
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The map label for this gene is rpoD2 [H]
Identifier: 94313583
GI number: 94313583
Start: 1293476
End: 1295329
Strand: Reverse
Name: rpoD2 [H]
Synonym: Rmet_4661
Alternate gene names: 94313583
Gene position: 1295329-1293476 (Counterclockwise)
Preceding gene: 94313584
Following gene: 94313576
Centisome position: 50.2
GC content: 58.41
Gene sequence:
>1854_bases ATGAATAACACCCACCCCGAGTCCTTGGTCGCGCTGGTTGCTTTAGGCAGCGAGCGGGGATTCCTGACGCACGGCGAGAT CAGCGACGCCCTTCCTGACAGTGACTTTGGGCAGATCCAACGCGTGTTGGAGGAGCTGGGCGTCCAGATCGTTGAACGGA CGTTGGCGAACGAACCCATCAGACTGGAGGAGCACGCGGACGCTCTGGTCGTGGATGAGGCGGTCATCGAGGAAGCGGCA GCGGCAGTCATGCAGGCCGATGGGCGTGAGGCACGAACTACCGACCCTGTGCAACTGTATCTGCGTGAAATTGGGACGAT TCCTTTGTTGACGCGAGAGGACGAGGTCGCGATTGCCAGGAGACATGAAATCGCGGTGACCGAAATGGTTGCCGAAATGG TCTCGTGTCCGCTCGTGGTTTATGAACTGCTCACGATGGCAGACGAAATCGAGCGAGGCGAGATGGCTGTCGACGACCTT ATCGACCATTGGGATGAGGTGCCTGCCGCCGCAGTCAATGTACCAGCTATCGAGATGAGTGAGGCGGTCCAGGATGATGA TCCAGCGCCTGGCGCTGCGGGTGTGGACCTCGAAGAGACGGATCCGGTCTCCACCGGCGCCTCGACGGCCCGATCATCGG CCGCATTGGAGAGTGAGGTGATAGCGCGACTTGCGATCGTTCGAGCCCGGTTCCTTGACTGGTCACGCCAATCCAGGCAG CAGGCAGCCACATCGGACGCCTTCGTTGCATTGAATGCGATTCGGGCCGAGTTGGCGGTCTATCACTTCAGTGCCGCTGC AGTCGCCCGTCTCGGCCAGGTGCTGTCCACTCACATCGCTGCAATTCAACAGGCCGAACGCCGTATCGCGGGGATCGTCA TTGATAGATGCGGTATCGCAAGAGAGCAATTCCTTCAGACGTTTCCGGGGCATGAAATCGATCTTGGCTGGTCGGAGGCG CTGGCGGCAGAGCGCATCAGTGGTGCTGCGGACGCGCTTGTGCGAGCCATCCCGGAAATTCAGGCAATTCAGCGGGAAAT GAGGGTGCTTGAGAACCGCGCGGCCATGTCACTTGGCGATCTGAAAAACCTCGGCCAGCGCGTTGCCAAAGCACGCGCGA GGGCTCAGGCCGCCAGGGACGAAATGATCAGGTCCAATCTCCGTCTCGTCGTTTCAAATGCCAAGAAGTATCTTCACCGC GGGATGCCTCTGCTGGATCTTATTCAGGAGGGCAATCTCGGGCTGATGCGAGCCGTAGACCGATTCGATTACCGCCGGGG CTACAAGTTCTCGACCTATGCCACGTGGTGGATTCGTCAGGGCATTACCCGCGCTATCGCGGACAAGGGGCCGACTATCC GGATTCCGATTCATCTGGTGGAGGTTGTGGGAAAGGTCAATCGTGCGTCACGGGAACTCTTTCATGAAACGGGAGAGGCC CCTGCACCGGAAGCTTTGGCCGCGCGTCTTTCCCTTTCCGTAAAAAAGGTCAAGGCGATTCTTGATCTGGTCAAAGAACC ACTGTCGCTGGAAATGTCGGTTGGCGAAAGCGGAGATGCCTTGCTATGGGATACGCTCACCGACGAATGTTCAGCTTCAC CGGATGAATCCGCAATGCGCGTGTCGCTCAGAAAGGATCTGGATGAGGCGTTGGCAACCCTGAAACCAAGGGAAGCGAAG ATTGTAAAGATGCGGTACGGCGTTGATGCCGGCACAGACTACACACTCGAAGAAATAGGCCAGCAGTATGGTCTTTCGCG TGAGCGAGTCCGCCAGCTCGAACGTCAGGCCCTGCGCAAGCTACGCAATCCCGATCGCGCACCTCGCTTGCAGGCGCATC GAGATATCCATTAG
Upstream 100 bases:
>100_bases CAAGCAAGATGGCGGCGGCAGCAATTATCGTGATCTGGACACTTTCCGCTTTCGCAATCGCGCATGCGCTCCCGCGCTTG CTGGCAAGCTTTTTTCATCC
Downstream 100 bases:
>100_bases GGTGCACGTTTACCTCTCGCCGGCAAAAATGGGGCCATGGAAAATGCTCGCCCAAGCTTGCAATAGGTGGTCCAAGCTCT TTCGGTTCCACGTGGGCACC
Product: RNA polymerase sigma 70 (sigma D) factor
Products: NA
Alternate protein names: Sigma-70 [H]
Number of amino acids: Translated: 617; Mature: 617
Protein sequence:
>617_residues MNNTHPESLVALVALGSERGFLTHGEISDALPDSDFGQIQRVLEELGVQIVERTLANEPIRLEEHADALVVDEAVIEEAA AAVMQADGREARTTDPVQLYLREIGTIPLLTREDEVAIARRHEIAVTEMVAEMVSCPLVVYELLTMADEIERGEMAVDDL IDHWDEVPAAAVNVPAIEMSEAVQDDDPAPGAAGVDLEETDPVSTGASTARSSAALESEVIARLAIVRARFLDWSRQSRQ QAATSDAFVALNAIRAELAVYHFSAAAVARLGQVLSTHIAAIQQAERRIAGIVIDRCGIAREQFLQTFPGHEIDLGWSEA LAAERISGAADALVRAIPEIQAIQREMRVLENRAAMSLGDLKNLGQRVAKARARAQAARDEMIRSNLRLVVSNAKKYLHR GMPLLDLIQEGNLGLMRAVDRFDYRRGYKFSTYATWWIRQGITRAIADKGPTIRIPIHLVEVVGKVNRASRELFHETGEA PAPEALAARLSLSVKKVKAILDLVKEPLSLEMSVGESGDALLWDTLTDECSASPDESAMRVSLRKDLDEALATLKPREAK IVKMRYGVDAGTDYTLEEIGQQYGLSRERVRQLERQALRKLRNPDRAPRLQAHRDIH
Sequences:
>Translated_617_residues MNNTHPESLVALVALGSERGFLTHGEISDALPDSDFGQIQRVLEELGVQIVERTLANEPIRLEEHADALVVDEAVIEEAA AAVMQADGREARTTDPVQLYLREIGTIPLLTREDEVAIARRHEIAVTEMVAEMVSCPLVVYELLTMADEIERGEMAVDDL IDHWDEVPAAAVNVPAIEMSEAVQDDDPAPGAAGVDLEETDPVSTGASTARSSAALESEVIARLAIVRARFLDWSRQSRQ QAATSDAFVALNAIRAELAVYHFSAAAVARLGQVLSTHIAAIQQAERRIAGIVIDRCGIAREQFLQTFPGHEIDLGWSEA LAAERISGAADALVRAIPEIQAIQREMRVLENRAAMSLGDLKNLGQRVAKARARAQAARDEMIRSNLRLVVSNAKKYLHR GMPLLDLIQEGNLGLMRAVDRFDYRRGYKFSTYATWWIRQGITRAIADKGPTIRIPIHLVEVVGKVNRASRELFHETGEA PAPEALAARLSLSVKKVKAILDLVKEPLSLEMSVGESGDALLWDTLTDECSASPDESAMRVSLRKDLDEALATLKPREAK IVKMRYGVDAGTDYTLEEIGQQYGLSRERVRQLERQALRKLRNPDRAPRLQAHRDIH >Mature_617_residues MNNTHPESLVALVALGSERGFLTHGEISDALPDSDFGQIQRVLEELGVQIVERTLANEPIRLEEHADALVVDEAVIEEAA AAVMQADGREARTTDPVQLYLREIGTIPLLTREDEVAIARRHEIAVTEMVAEMVSCPLVVYELLTMADEIERGEMAVDDL IDHWDEVPAAAVNVPAIEMSEAVQDDDPAPGAAGVDLEETDPVSTGASTARSSAALESEVIARLAIVRARFLDWSRQSRQ QAATSDAFVALNAIRAELAVYHFSAAAVARLGQVLSTHIAAIQQAERRIAGIVIDRCGIAREQFLQTFPGHEIDLGWSEA LAAERISGAADALVRAIPEIQAIQREMRVLENRAAMSLGDLKNLGQRVAKARARAQAARDEMIRSNLRLVVSNAKKYLHR GMPLLDLIQEGNLGLMRAVDRFDYRRGYKFSTYATWWIRQGITRAIADKGPTIRIPIHLVEVVGKVNRASRELFHETGEA PAPEALAARLSLSVKKVKAILDLVKEPLSLEMSVGESGDALLWDTLTDECSASPDESAMRVSLRKDLDEALATLKPREAK IVKMRYGVDAGTDYTLEEIGQQYGLSRERVRQLERQALRKLRNPDRAPRLQAHRDIH
Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]
COG id: COG0568
COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the sigma-70 factor family [H]
Homologues:
Organism=Escherichia coli, GI1789448, Length=638, Percent_Identity=34.1692789968652, Blast_Score=370, Evalue=1e-103, Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=42.4778761061947, Blast_Score=186, Evalue=4e-48, Organism=Escherichia coli, GI1789871, Length=239, Percent_Identity=29.7071129707113, Blast_Score=84, Evalue=2e-17,
Paralogues:
None
Copy number: 700 (log & stationary phase) [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014284 - InterPro: IPR000943 - InterPro: IPR009042 - InterPro: IPR007627 - InterPro: IPR007624 - InterPro: IPR007630 - InterPro: IPR007631 - InterPro: IPR007127 - InterPro: IPR013325 - InterPro: IPR013324 - InterPro: IPR012760 - InterPro: IPR011991 [H]
Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]
EC number: NA
Molecular weight: Translated: 68018; Mature: 68018
Theoretical pI: Translated: 4.95; Mature: 4.95
Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.9 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNNTHPESLVALVALGSERGFLTHGEISDALPDSDFGQIQRVLEELGVQIVERTLANEPI CCCCCHHHHHHHHHCCCCCCCEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE RLEEHADALVVDEAVIEEAAAAVMQADGREARTTDPVQLYLREIGTIPLLTREDEVAIAR EEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHH RHEIAVTEMVAEMVSCPLVVYELLTMADEIERGEMAVDDLIDHWDEVPAAAVNVPAIEMS HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCHHHH EAVQDDDPAPGAAGVDLEETDPVSTGASTARSSAALESEVIARLAIVRARFLDWSRQSRQ HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QAATSDAFVALNAIRAELAVYHFSAAAVARLGQVLSTHIAAIQQAERRIAGIVIDRCGIA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH REQFLQTFPGHEIDLGWSEALAAERISGAADALVRAIPEIQAIQREMRVLENRAAMSLGD HHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LKNLGQRVAKARARAQAARDEMIRSNLRLVVSNAKKYLHRGMPLLDLIQEGNLGLMRAVD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHH RFDYRRGYKFSTYATWWIRQGITRAIADKGPTIRIPIHLVEVVGKVNRASRELFHETGEA HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCC PAPEALAARLSLSVKKVKAILDLVKEPLSLEMSVGESGDALLWDTLTDECSASPDESAMR CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCHHHHCCCCCCHHHHH VSLRKDLDEALATLKPREAKIVKMRYGVDAGTDYTLEEIGQQYGLSRERVRQLERQALRK HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH LRNPDRAPRLQAHRDIH HCCCCCCCCCCCCCCCC >Mature Secondary Structure MNNTHPESLVALVALGSERGFLTHGEISDALPDSDFGQIQRVLEELGVQIVERTLANEPI CCCCCHHHHHHHHHCCCCCCCEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE RLEEHADALVVDEAVIEEAAAAVMQADGREARTTDPVQLYLREIGTIPLLTREDEVAIAR EEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHH RHEIAVTEMVAEMVSCPLVVYELLTMADEIERGEMAVDDLIDHWDEVPAAAVNVPAIEMS HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHCCCCCHHHH EAVQDDDPAPGAAGVDLEETDPVSTGASTARSSAALESEVIARLAIVRARFLDWSRQSRQ HHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QAATSDAFVALNAIRAELAVYHFSAAAVARLGQVLSTHIAAIQQAERRIAGIVIDRCGIA HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH REQFLQTFPGHEIDLGWSEALAAERISGAADALVRAIPEIQAIQREMRVLENRAAMSLGD HHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LKNLGQRVAKARARAQAARDEMIRSNLRLVVSNAKKYLHRGMPLLDLIQEGNLGLMRAVD HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHH RFDYRRGYKFSTYATWWIRQGITRAIADKGPTIRIPIHLVEVVGKVNRASRELFHETGEA HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCC PAPEALAARLSLSVKKVKAILDLVKEPLSLEMSVGESGDALLWDTLTDECSASPDESAMR CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCHHHHCCCCCCHHHHH VSLRKDLDEALATLKPREAKIVKMRYGVDAGTDYTLEEIGQQYGLSRERVRQLERQALRK HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH LRNPDRAPRLQAHRDIH HCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: DNA [C]
Specific reaction: Protein + DNA = Protein-DNA [C]
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA