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The map label for this gene is purR [H]

Identifier: 94313569

GI number: 94313569

Start: 1277165

End: 1278184

Strand: Direct

Name: purR [H]

Synonym: Rmet_4647

Alternate gene names: 94313569

Gene position: 1277165-1278184 (Clockwise)

Preceding gene: 291481533

Following gene: 94313572

Centisome position: 49.5

GC content: 64.9

Gene sequence:

>1020_bases
ATGACGGGAAAGGCTTCTGTGCGCGATGTGGCGCAGGCGGCGGGGGTATCGGTCGGTAGTGTGTCGCGCGTCCTGAACGG
CAACGGCTATGCGAGCCAGGAACTGCGCGGCCGCGTGCTGGCCGCGGTGAAAGCACTCGACTATGAGCCGAGCTTCGCCG
CGCGCCATCTGCGTACCGGGCGCAGTCGAACGGTCGGCTATCTGACAACCGATATCAGCAATCCGCTGCTGGCGGCCCAT
TTCAGCGAAGTCGAGCAGCACATGCACGCCGCGGGCTATTCGATGCTGATCGGCACCACGCGGAACCAGCACCACCGCGA
CCGCCTGCTGGTCGAACTGTTCGAGACGCGCCGGCTTGAGGGCATCATCGCGTCACTGAGCATGGAGAGTTCGGACCCGG
CCGAAGACCTGTTCGCCAAGGCGGACCTGCCAATCGTCATCATGGATCGCGAAACGGCATCGCCCAGGGATTGCGTGCTG
ATCGACCATCGAACCGGACTGAGCCTGGCGGTGCGCTACCTGAAGCAACTCGGCCATCGGCGCATTGCGTTCTTCGGGCC
AAAGGAGAGCATCCGTCCGGGCCGTGAGAAGCTGCTCGGCTATCAGGAAGGACTGGCCGAGGTCGGGCTTTTATTCGATC
CCGCGCTGGCGTGCACCCTGCCCTCGATGGACTCCTCGCGCGCCGCGATGAAGCAGATGCTGGCGCTGAAGCAACCGCCG
ACGGCGGTGATCGCGCTGGGCACGCGCCTGCTTTCAGGGGCGCTCTATGAGGCGCGCAAGGCCGGGCTGGAAGTGCCGCA
CGATCTGTCCGTCATCGCGCTTGGCACGCCGGAAACGCTTGAACTGATGTATCCGCCCCCGACGATGCTCCGCTTCAACA
CAGCGCTTTCGGCAAAGACCGCGGCGAACCTGATGCTCGACCGGCTCGAAGGCCACTACGCTGGCGAGGCGCGGCGCGTG
GTGATACCGATGGATCTTATGTTGGGAGAATCGTGCGCACCGGTGCGTCCGGGGCGGTAG

Upstream 100 bases:

>100_bases
CCGTTGTAGTTCCAGATGTGGAATCGTTTTCCGTGTGGTATTAATTTACCCAGCAAGGAAATCGGCGTCCAACTGTGGAA
CGTTTTCATTAGGGAAGACC

Downstream 100 bases:

>100_bases
CGTGCCCAGTACGCCCGGAATGTCAGTCCGCGAATTCAGCGATTGCCGTCGCCGCGCGTGGCAGGAGCGCCTGGCACATC
GTTCAACTCGTAGCTGGTGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTGKASVRDVAQAAGVSVGSVSRVLNGNGYASQELRGRVLAAVKALDYEPSFAARHLRTGRSRTVGYLTTDISNPLLAAH
FSEVEQHMHAAGYSMLIGTTRNQHHRDRLLVELFETRRLEGIIASLSMESSDPAEDLFAKADLPIVIMDRETASPRDCVL
IDHRTGLSLAVRYLKQLGHRRIAFFGPKESIRPGREKLLGYQEGLAEVGLLFDPALACTLPSMDSSRAAMKQMLALKQPP
TAVIALGTRLLSGALYEARKAGLEVPHDLSVIALGTPETLELMYPPPTMLRFNTALSAKTAANLMLDRLEGHYAGEARRV
VIPMDLMLGESCAPVRPGR

Sequences:

>Translated_339_residues
MTGKASVRDVAQAAGVSVGSVSRVLNGNGYASQELRGRVLAAVKALDYEPSFAARHLRTGRSRTVGYLTTDISNPLLAAH
FSEVEQHMHAAGYSMLIGTTRNQHHRDRLLVELFETRRLEGIIASLSMESSDPAEDLFAKADLPIVIMDRETASPRDCVL
IDHRTGLSLAVRYLKQLGHRRIAFFGPKESIRPGREKLLGYQEGLAEVGLLFDPALACTLPSMDSSRAAMKQMLALKQPP
TAVIALGTRLLSGALYEARKAGLEVPHDLSVIALGTPETLELMYPPPTMLRFNTALSAKTAANLMLDRLEGHYAGEARRV
VIPMDLMLGESCAPVRPGR
>Mature_338_residues
TGKASVRDVAQAAGVSVGSVSRVLNGNGYASQELRGRVLAAVKALDYEPSFAARHLRTGRSRTVGYLTTDISNPLLAAHF
SEVEQHMHAAGYSMLIGTTRNQHHRDRLLVELFETRRLEGIIASLSMESSDPAEDLFAKADLPIVIMDRETASPRDCVLI
DHRTGLSLAVRYLKQLGHRRIAFFGPKESIRPGREKLLGYQEGLAEVGLLFDPALACTLPSMDSSRAAMKQMLALKQPPT
AVIALGTRLLSGALYEARKAGLEVPHDLSVIALGTPETLELMYPPPTMLRFNTALSAKTAANLMLDRLEGHYAGEARRVV
IPMDLMLGESCAPVRPGR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=323, Percent_Identity=30.030959752322, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=29.4670846394984, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789202, Length=292, Percent_Identity=30.4794520547945, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790194, Length=325, Percent_Identity=28.9230769230769, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788474, Length=321, Percent_Identity=28.3489096573209, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789068, Length=273, Percent_Identity=29.3040293040293, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786540, Length=309, Percent_Identity=26.537216828479, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787906, Length=343, Percent_Identity=27.6967930029155, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787580, Length=292, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786268, Length=290, Percent_Identity=24.8275862068966, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI48994940, Length=333, Percent_Identity=24.9249249249249, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36892; Mature: 36761

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKASVRDVAQAAGVSVGSVSRVLNGNGYASQELRGRVLAAVKALDYEPSFAARHLRTG
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCC
RSRTVGYLTTDISNPLLAAHFSEVEQHMHAAGYSMLIGTTRNQHHRDRLLVELFETRRLE
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
GIIASLSMESSDPAEDLFAKADLPIVIMDRETASPRDCVLIDHRTGLSLAVRYLKQLGHR
HHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
RIAFFGPKESIRPGREKLLGYQEGLAEVGLLFDPALACTLPSMDSSRAAMKQMLALKQPP
EEEEECCHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCHHHHHHHHHHHCCCC
TAVIALGTRLLSGALYEARKAGLEVPHDLSVIALGTPETLELMYPPPTMLRFNTALSAKT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCEEECHHHHHHH
AANLMLDRLEGHYAGEARRVVIPMDLMLGESCAPVRPGR
HHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TGKASVRDVAQAAGVSVGSVSRVLNGNGYASQELRGRVLAAVKALDYEPSFAARHLRTG
CCCHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCC
RSRTVGYLTTDISNPLLAAHFSEVEQHMHAAGYSMLIGTTRNQHHRDRLLVELFETRRLE
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
GIIASLSMESSDPAEDLFAKADLPIVIMDRETASPRDCVLIDHRTGLSLAVRYLKQLGHR
HHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCC
RIAFFGPKESIRPGREKLLGYQEGLAEVGLLFDPALACTLPSMDSSRAAMKQMLALKQPP
EEEEECCHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCHHHHHHHHHHHCCCC
TAVIALGTRLLSGALYEARKAGLEVPHDLSVIALGTPETLELMYPPPTMLRFNTALSAKT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEECCCCCEEECHHHHHHH
AANLMLDRLEGHYAGEARRVVIPMDLMLGESCAPVRPGR
HHHHHHHHHCCCCCCCCEEEEEEHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA