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The map label for this gene is metY [H]

Identifier: 94313528

GI number: 94313528

Start: 1230923

End: 1232218

Strand: Direct

Name: metY [H]

Synonym: Rmet_4606

Alternate gene names: 94313528

Gene position: 1230923-1232218 (Clockwise)

Preceding gene: 94313526

Following gene: 94313531

Centisome position: 47.71

GC content: 62.58

Gene sequence:

>1296_bases
ATGTCTGAAAACTGGAAATTCGAAACGCGTTCGGTCCACGCGGGATATAGCCCTGACCCTACGACTAAGGCAGTGGCAGT
TCCGATCTACCAGACGGCGGCCTACGCCTTCGACAATGCGCAGCATGGCGCGGACCTGTTCGATCTGAAGGTGCCGGGGA
ATATCTACACCCGGATCATGAACCCGACGACCGACGTGCTCGAGAAGCGGATCGCGGCGCTGGAAGGCGGTGTCGGGGCG
CTGGCGGTCGCATCCGGCCAGGCGGCGGTCACCGCGGCGATCCAGACGATCACCGAGGCCGGCGACAATATCGTCGCTTC
CTCGGCGCTCTACGGCGGCACCTACAATCTGCTGGCGCATACCATGCCGCAGTTTGGCGTTACGACACGGTTTGCCGATC
ACCGGTGCCCGGAAAGCTTCGAGGCACTAATCGACGATCGGACCAAGGCTGTGTTTGTGGAGTCGATCGGTAATCCGGCG
GGTAACGTGACGGATATCGCGAAGATTGCGGCGATCGCAAAGCGGCATGGTGTGCCAGTCATCGTCGACAATACCGTCGC
CACGCCCTATCTCCTTCGGCCGTTCGAGCATGGCGCCGATATCGTCGTTCACTCGCTGACGAAGTACCTCGGCGGTCATG
GCACGAGCATTGGCGGAGCCATCGTCGATTCGGGCAAGTTCCCGTGGGCGAAGTACCCCTCTCGCTTTCCGAAGCTCAAT
ACCCCGGATGCCAGCTATCACGGCGTGACGTATACGGAAGCCTTCGGCCCCGCGGCATACATCGGCCGGGCTCGCGTGGT
CCCGCTGCGGAACATGGGCGCGGCGCTATCGCCATTCAATGCCTTCCTGATCCTGCAAGGCATCGAAACGCTTGGCCTGC
GCATGGATCGGATCAACGACAACACGCTCGCGGTCGCCCGGCATCTTAGCCAGCACGCCAAGGTGGGCTGGGTCAACTAC
GCGGGGCTCGAGGACAACCAGGACTACGCGCTCGCCAAGAAGTACATGAATGGCAAGGCTTCGGGCCTGCTCACGTTCGG
TGTCAAGGCCGCCGATGGCGATGGGCGGGCGGCTGGTGCCCGGTTCCTGGATGCGCTGCAGCTGTTCACGCGCCTGGTGA
ACATCGGCGATGTGCGGTCGCTGGCCACGCACCCTGCTTCCACGACGCATCGCCAGCTTTCCCCGGAAGAGTTGGCACAG
ACGGGCGTGGGACTGGATACGGTTCGCCTCTGCGTTGGCATCGAGCACATCGACGACCTGCTGGCTGATATCGACCAGGC
GCTGCAGGCGGTATAA

Upstream 100 bases:

>100_bases
CCTTGGTACCACCTCTCCGGGTAACATGCCCCTGATCTACGGAGGTGCTAAGCCACCGCGAGAAGGCTGTCTTTTGGTGA
AACCTGGGAGTGCTTGATCA

Downstream 100 bases:

>100_bases
CCGTCGGCGCTGTCGCGCGGCATTGGTGGGCGGGAAGCCACGCTTCTCCGCCCACTTTCTACTCTTGCCGCCCTGCCCTC
AGATCTTGATGCGCAACCGC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSENWKFETRSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAALEGGVGA
LAVASGQAAVTAAIQTITEAGDNIVASSALYGGTYNLLAHTMPQFGVTTRFADHRCPESFEALIDDRTKAVFVESIGNPA
GNVTDIAKIAAIAKRHGVPVIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIGGAIVDSGKFPWAKYPSRFPKLN
TPDASYHGVTYTEAFGPAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLGLRMDRINDNTLAVARHLSQHAKVGWVNY
AGLEDNQDYALAKKYMNGKASGLLTFGVKAADGDGRAAGARFLDALQLFTRLVNIGDVRSLATHPASTTHRQLSPEELAQ
TGVGLDTVRLCVGIEHIDDLLADIDQALQAV

Sequences:

>Translated_431_residues
MSENWKFETRSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAALEGGVGA
LAVASGQAAVTAAIQTITEAGDNIVASSALYGGTYNLLAHTMPQFGVTTRFADHRCPESFEALIDDRTKAVFVESIGNPA
GNVTDIAKIAAIAKRHGVPVIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIGGAIVDSGKFPWAKYPSRFPKLN
TPDASYHGVTYTEAFGPAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLGLRMDRINDNTLAVARHLSQHAKVGWVNY
AGLEDNQDYALAKKYMNGKASGLLTFGVKAADGDGRAAGARFLDALQLFTRLVNIGDVRSLATHPASTTHRQLSPEELAQ
TGVGLDTVRLCVGIEHIDDLLADIDQALQAV
>Mature_430_residues
SENWKFETRSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAALEGGVGAL
AVASGQAAVTAAIQTITEAGDNIVASSALYGGTYNLLAHTMPQFGVTTRFADHRCPESFEALIDDRTKAVFVESIGNPAG
NVTDIAKIAAIAKRHGVPVIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIGGAIVDSGKFPWAKYPSRFPKLNT
PDASYHGVTYTEAFGPAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLGLRMDRINDNTLAVARHLSQHAKVGWVNYA
GLEDNQDYALAKKYMNGKASGLLTFGVKAADGDGRAAGARFLDALQLFTRLVNIGDVRSLATHPASTTHRQLSPEELAQT
GVGLDTVRLCVGIEHIDDLLADIDQALQAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=33.2552693208431, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=30.2107728337237, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=29.3427230046948, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790375, Length=427, Percent_Identity=30.9133489461358, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=27.0588235294118, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=439, Percent_Identity=29.8405466970387, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=427, Percent_Identity=33.4894613583138, Blast_Score=184, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=431, Percent_Identity=34.1067285382831, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=49.7695852534562, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6319307, Length=436, Percent_Identity=31.1926605504587, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=28.0898876404494, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=355, Percent_Identity=27.3239436619718, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922554, Length=429, Percent_Identity=32.4009324009324, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45997; Mature: 45865

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENWKFETRSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIM
CCCCCCEECEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEECCCHHEEEEC
NPTTDVLEKRIAALEGGVGALAVASGQAAVTAAIQTITEAGDNIVASSALYGGTYNLLAH
CCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHH
TMPQFGVTTRFADHRCPESFEALIDDRTKAVFVESIGNPAGNVTDIAKIAAIAKRHGVPV
HCCCCCCEECCHHCCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCE
IVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIGGAIVDSGKFPWAKYPSRFPKLN
EECCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
TPDASYHGVTYTEAFGPAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLGLRMDRIND
CCCCCCCCCEEHHHCCCHHHHCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
NTLAVARHLSQHAKVGWVNYAGLEDNQDYALAKKYMNGKASGLLTFGVKAADGDGRAAGA
CHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHH
RFLDALQLFTRLVNIGDVRSLATHPASTTHRQLSPEELAQTGVGLDTVRLCVGIEHIDDL
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
LADIDQALQAV
HHHHHHHHHCC
>Mature Secondary Structure 
SENWKFETRSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIM
CCCCCEECEEEECCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEECCCHHEEEEC
NPTTDVLEKRIAALEGGVGALAVASGQAAVTAAIQTITEAGDNIVASSALYGGTYNLLAH
CCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHH
TMPQFGVTTRFADHRCPESFEALIDDRTKAVFVESIGNPAGNVTDIAKIAAIAKRHGVPV
HCCCCCCEECCHHCCCCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCE
IVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIGGAIVDSGKFPWAKYPSRFPKLN
EECCCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
TPDASYHGVTYTEAFGPAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLGLRMDRIND
CCCCCCCCCEEHHHCCCHHHHCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
NTLAVARHLSQHAKVGWVNYAGLEDNQDYALAKKYMNGKASGLLTFGVKAADGDGRAAGA
CHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHH
RFLDALQLFTRLVNIGDVRSLATHPASTTHRQLSPEELAQTGVGLDTVRLCVGIEHIDDL
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHH
LADIDQALQAV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]