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The map label for this gene is metE [H]

Identifier: 94313486

GI number: 94313486

Start: 1172799

End: 1175210

Strand: Direct

Name: metE [H]

Synonym: Rmet_4564

Alternate gene names: 94313486

Gene position: 1172799-1175210 (Clockwise)

Preceding gene: 94313484

Following gene: 94313487

Centisome position: 45.46

GC content: 65.71

Gene sequence:

>2412_bases
ATGGCACGCACACATATCCTCGGATTTCCGCGCATTGGCGCGCGTCGTGAGCTGAAATTCGCGCAGGAAGCGTTCTGGCG
CGGCGAAACGCCCGAAGCCGCCTTGCGCGAAACCGGCACCACGCTGCGACGTCGTCACTGGCAGCTGCAGGCCGATGCCG
GCCTGGCGACCGTGGCTGCCGGCGACTTCGCCTGGTATGACCAGATGCTCGGACTGACCGCACTGCTGGGCGCGCTGCCG
AAGCGTTTCGGCTTTGACGCCGCATCGCTGACGCTGGCCCAGTATTACGAACTGGCACGCGGCAATGCCGCCCAGCCGGC
CATGGAAATGACCAAGTGGTTCGATACGAACTACCACTACCTCGTCCCCGAACTCGATGCGGACAGCACGTTCGATGGCG
GCCCCACATGGTTCCTCGATGAAGTCGATGAAGCCCTGGCACTGGGCCTGGCTACGCGCCCCGTCCTGATCGGCCCGGTC
ACTTACCTGTACCTGTCGAAGTCGCACGTCCCCGGGTTCGATCGCCTCACCTTGCTGCCCAAGGTCGTGCAGGCCTACCG
CCGCATCCTGGCGCAACTGCGCGAGCGCGGTATCGAATGGGTGCAGATCGACGAGCCGGTACTGTGCCTCGATATCGACG
CCGAGTGGCTCGACGCCATTGATCGCGCTTATGTCGATCTGGTCGATCTGGTCGATCTGGCCGACGCGGGTCCGAAGGTC
CTGCTGGCTACCTACTTCGACACCGCCGCTCCCCATGCGGCACGGGCCGCCGCGCTGCCGGTGCATGGCTTCCATGTCGA
TCTGGTGCGCGCGCCCGAGCAACTGGAAACCTGGCTGCAGGTGCTGCCCGCCAACGCGGTGCTGTCGGCAGGTGTGATCG
ACGGCCGCAACATCTGGCGCGCGGACCTGCGTGCCGTGCTGGCGACGCTCGCCCCGCTTCACGCACAACTCGGCGACCGT
CTCTGGATCGCCCCGTCCTGCTCGCTGCTGCACGTGCCCGTATCGCTCGATGCCGAACAACGCCTCGATCCCGAACTGAA
ATCGTGGCTGGCCTTCGCTACAGAAAAGCTCGAGGAACTGCGCACGCTGGCCGTGGCGCTGAATCAGGGCAAAGCCGCAG
CGGCGACGCAGCTCGCGGCATCAGACGCAGCACGCGAATCGCGCCGCACGTCGCGCCGCGTGGTCAACCAGCGGGTACAA
GGTCGCCTGGCCGCTGTCACCGCCGACATGGCCGATCGCGCCAGCGCCTTCGATATCCGCATCGAACGCCAGCGCGAGGC
GCTGCAACTGCCCGCGCTGCCCACCACCACGATCGGTTCGTTCCCGCAGACCACGGCGATCCGGCAAACCCGCGCGGCAT
ACAAGCGTGGCGAGATCGGCGCGCTGGACTACCTGCAGCGCATCCGTGGCGAGATCGAACTGGCGGTGCGCAAGCAGGAA
GCACTTGGCATCGACGTGCTGGTGCATGGCGAGGCCGAGCGTAACGACATGGTCGAGTACTTCGGTGAACAACTCTGCGG
CTACGCATTCACCGAGAACGGCTGGGTGCAAAGCTATGGCTCGCGTTGCGTCAAGCCGCCGGTGATCTATGGCGATGTGT
ATCGCCCCGAGCCGATGACCGTGGACACGGCACGCTACGCACAGTCGCTGACCGACAAGCCGATGAAGGGCATGTTGACC
GGCCCGGTGACAATGCTGCAATGGTCATTCGTGCGCGACGACCAGCCGCGTGCCACCACGGCGCGTCAACTGGCCCTGGC
GATCCGCGACGAGGTACGCGATCTGGAAGCGGCCGGCATCCGCATCATCCAGATCGACGAACCGGCACTGCGCGAAGGCC
TGCCCCTGCGCCGCGACGACTGGACCGCGTACCTCGAGTGGGCCGTCAATGCCTTCCGGCTGTCGGCCGCCGGCGTATCG
GACCAGACCCAGATCCACACCCATATGTGCTACGCGGAGTTCAACGACATCCTGCCGGCCATCGCAGCCATGGATGCCGA
CGTGATCACGATCGAGACCTCGCGCTCGGCGATGGAATTGCTGGAAGGCTTCGGGGAGTTCGACTACCCGAATGAAATCG
GACCGGGCGTCTACGACATCCACTCGCCGCGCGTGCCGACGGTGGAAGCCATCACCCGGCTGCTCGAGCGGGCGTGCGAA
GTGATTCCGCCAGAGCGGCTGTGGGTGAACCCGGACTGCGGTCTCAAGACGCGCGGCTGGCCGGAAACGGAAGCGGCGCT
GGCGAACATGATCACGGCAACGCGCCAGTTGCGCGGCAAGCTGGCAAGCCAGCCACCCGTGACTTGGAAACGTGTGACCC
AGCCTGCCCCCGGAAGTGCGCGCAGCGCGGCAGCGGCGCACGCTCACGGTACTGCCTGCGACCCTAACTGCGCGGGACAA
CTTTCGATATAA

Upstream 100 bases:

>100_bases
TGAATTTCATTCACTCTTTGTTTAGAACTTATCGCTTGATTCATTTTACCCGCAATCCGAAACTGTGCGCCATATCAGAT
ACCGGACTTGGGAGTAACGG

Downstream 100 bases:

>100_bases
GGCAAGAAGGTCCGTTGCGTCGCTGTGTCGCATGGCGCACGGATTCTCCCCTCCGTATCCTTGCGGTCGAGCCAAAATAA
GAGCATATCTGGCCCACGTT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MARTHILGFPRIGARRELKFAQEAFWRGETPEAALRETGTTLRRRHWQLQADAGLATVAAGDFAWYDQMLGLTALLGALP
KRFGFDAASLTLAQYYELARGNAAQPAMEMTKWFDTNYHYLVPELDADSTFDGGPTWFLDEVDEALALGLATRPVLIGPV
TYLYLSKSHVPGFDRLTLLPKVVQAYRRILAQLRERGIEWVQIDEPVLCLDIDAEWLDAIDRAYVDLVDLVDLADAGPKV
LLATYFDTAAPHAARAAALPVHGFHVDLVRAPEQLETWLQVLPANAVLSAGVIDGRNIWRADLRAVLATLAPLHAQLGDR
LWIAPSCSLLHVPVSLDAEQRLDPELKSWLAFATEKLEELRTLAVALNQGKAAAATQLAASDAARESRRTSRRVVNQRVQ
GRLAAVTADMADRASAFDIRIERQREALQLPALPTTTIGSFPQTTAIRQTRAAYKRGEIGALDYLQRIRGEIELAVRKQE
ALGIDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTDKPMKGMLT
GPVTMLQWSFVRDDQPRATTARQLALAIRDEVRDLEAAGIRIIQIDEPALREGLPLRRDDWTAYLEWAVNAFRLSAAGVS
DQTQIHTHMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGEFDYPNEIGPGVYDIHSPRVPTVEAITRLLERACE
VIPPERLWVNPDCGLKTRGWPETEAALANMITATRQLRGKLASQPPVTWKRVTQPAPGSARSAAAAHAHGTACDPNCAGQ
LSI

Sequences:

>Translated_803_residues
MARTHILGFPRIGARRELKFAQEAFWRGETPEAALRETGTTLRRRHWQLQADAGLATVAAGDFAWYDQMLGLTALLGALP
KRFGFDAASLTLAQYYELARGNAAQPAMEMTKWFDTNYHYLVPELDADSTFDGGPTWFLDEVDEALALGLATRPVLIGPV
TYLYLSKSHVPGFDRLTLLPKVVQAYRRILAQLRERGIEWVQIDEPVLCLDIDAEWLDAIDRAYVDLVDLVDLADAGPKV
LLATYFDTAAPHAARAAALPVHGFHVDLVRAPEQLETWLQVLPANAVLSAGVIDGRNIWRADLRAVLATLAPLHAQLGDR
LWIAPSCSLLHVPVSLDAEQRLDPELKSWLAFATEKLEELRTLAVALNQGKAAAATQLAASDAARESRRTSRRVVNQRVQ
GRLAAVTADMADRASAFDIRIERQREALQLPALPTTTIGSFPQTTAIRQTRAAYKRGEIGALDYLQRIRGEIELAVRKQE
ALGIDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTDKPMKGMLT
GPVTMLQWSFVRDDQPRATTARQLALAIRDEVRDLEAAGIRIIQIDEPALREGLPLRRDDWTAYLEWAVNAFRLSAAGVS
DQTQIHTHMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGEFDYPNEIGPGVYDIHSPRVPTVEAITRLLERACE
VIPPERLWVNPDCGLKTRGWPETEAALANMITATRQLRGKLASQPPVTWKRVTQPAPGSARSAAAAHAHGTACDPNCAGQ
LSI
>Mature_802_residues
ARTHILGFPRIGARRELKFAQEAFWRGETPEAALRETGTTLRRRHWQLQADAGLATVAAGDFAWYDQMLGLTALLGALPK
RFGFDAASLTLAQYYELARGNAAQPAMEMTKWFDTNYHYLVPELDADSTFDGGPTWFLDEVDEALALGLATRPVLIGPVT
YLYLSKSHVPGFDRLTLLPKVVQAYRRILAQLRERGIEWVQIDEPVLCLDIDAEWLDAIDRAYVDLVDLVDLADAGPKVL
LATYFDTAAPHAARAAALPVHGFHVDLVRAPEQLETWLQVLPANAVLSAGVIDGRNIWRADLRAVLATLAPLHAQLGDRL
WIAPSCSLLHVPVSLDAEQRLDPELKSWLAFATEKLEELRTLAVALNQGKAAAATQLAASDAARESRRTSRRVVNQRVQG
RLAAVTADMADRASAFDIRIERQREALQLPALPTTTIGSFPQTTAIRQTRAAYKRGEIGALDYLQRIRGEIELAVRKQEA
LGIDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTDKPMKGMLTG
PVTMLQWSFVRDDQPRATTARQLALAIRDEVRDLEAAGIRIIQIDEPALREGLPLRRDDWTAYLEWAVNAFRLSAAGVSD
QTQIHTHMCYAEFNDILPAIAAMDADVITIETSRSAMELLEGFGEFDYPNEIGPGVYDIHSPRVPTVEAITRLLERACEV
IPPERLWVNPDCGLKTRGWPETEAALANMITATRQLRGKLASQPPVTWKRVTQPAPGSARSAAAAHAHGTACDPNCAGQL
SI

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=760, Percent_Identity=57.8947368421053, Blast_Score=858, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=45.6774193548387, Blast_Score=680, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 88817; Mature: 88686

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTHILGFPRIGARRELKFAQEAFWRGETPEAALRETGTTLRRRHWQLQADAGLATVAA
CCCCEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEC
GDFAWYDQMLGLTALLGALPKRFGFDAASLTLAQYYELARGNAAQPAMEMTKWFDTNYHY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEE
LVPELDADSTFDGGPTWFLDEVDEALALGLATRPVLIGPVTYLYLSKSHVPGFDRLTLLP
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCHHHHHHH
KVVQAYRRILAQLRERGIEWVQIDEPVLCLDIDAEWLDAIDRAYVDLVDLVDLADAGPKV
HHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCE
LLATYFDTAAPHAARAAALPVHGFHVDLVRAPEQLETWLQVLPANAVLSAGVIDGRNIWR
EEEEHHCCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHH
ADLRAVLATLAPLHAQLGDRLWIAPSCSLLHVPVSLDAEQRLDPELKSWLAFATEKLEEL
HHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHH
RTLAVALNQGKAAAATQLAASDAARESRRTSRRVVNQRVQGRLAAVTADMADRASAFDIR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEE
IERQREALQLPALPTTTIGSFPQTTAIRQTRAAYKRGEIGALDYLQRIRGEIELAVRKQE
EECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHEEEEEHHH
ALGIDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMT
HCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCHHHHHCCCCCCCCEEECCCCCCCCCE
VDTARYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQPRATTARQLALAIRDEVRDLEAAGI
ECHHHHHHHHCCCCCCCCCCCCHHHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
RIIQIDEPALREGLPLRRDDWTAYLEWAVNAFRLSAAGVSDQTQIHTHMCYAEFNDILPA
EEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHH
IAAMDADVITIETSRSAMELLEGFGEFDYPNEIGPGVYDIHSPRVPTVEAITRLLERACE
HHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHH
VIPPERLWVNPDCGLKTRGWPETEAALANMITATRQLRGKLASQPPVTWKRVTQPAPGSA
HCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCC
RSAAAAHAHGTACDPNCAGQLSI
HHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARTHILGFPRIGARRELKFAQEAFWRGETPEAALRETGTTLRRRHWQLQADAGLATVAA
CCCEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCEEEEEC
GDFAWYDQMLGLTALLGALPKRFGFDAASLTLAQYYELARGNAAQPAMEMTKWFDTNYHY
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEE
LVPELDADSTFDGGPTWFLDEVDEALALGLATRPVLIGPVTYLYLSKSHVPGFDRLTLLP
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCCHHHHHHH
KVVQAYRRILAQLRERGIEWVQIDEPVLCLDIDAEWLDAIDRAYVDLVDLVDLADAGPKV
HHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCE
LLATYFDTAAPHAARAAALPVHGFHVDLVRAPEQLETWLQVLPANAVLSAGVIDGRNIWR
EEEEHHCCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHCCCCCCCHHHH
ADLRAVLATLAPLHAQLGDRLWIAPSCSLLHVPVSLDAEQRLDPELKSWLAFATEKLEEL
HHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHH
RTLAVALNQGKAAAATQLAASDAARESRRTSRRVVNQRVQGRLAAVTADMADRASAFDIR
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEE
IERQREALQLPALPTTTIGSFPQTTAIRQTRAAYKRGEIGALDYLQRIRGEIELAVRKQE
EECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHEEEEEHHH
ALGIDVLVHGEAERNDMVEYFGEQLCGYAFTENGWVQSYGSRCVKPPVIYGDVYRPEPMT
HCCEEEEEECCCCHHHHHHHHHHHHCCCEECCCCHHHHHCCCCCCCCEEECCCCCCCCCE
VDTARYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQPRATTARQLALAIRDEVRDLEAAGI
ECHHHHHHHHCCCCCCCCCCCCHHHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCE
RIIQIDEPALREGLPLRRDDWTAYLEWAVNAFRLSAAGVSDQTQIHTHMCYAEFNDILPA
EEEEECCHHHHCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHH
IAAMDADVITIETSRSAMELLEGFGEFDYPNEIGPGVYDIHSPRVPTVEAITRLLERACE
HHHCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHH
VIPPERLWVNPDCGLKTRGWPETEAALANMITATRQLRGKLASQPPVTWKRVTQPAPGSA
HCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCC
RSAAAAHAHGTACDPNCAGQLSI
HHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA